Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites

Autores
Gholipour, Yousef; Erra Balsells, Rosa; Hiraoka, Kenzo; Nonami, Hiroshi
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A modified cell pressure probe and an online Orbitrap mass spectrometer were used to sample in situ plant single cells without any additional manipulation. The cell pressure probe, a quartz capillary tip filled with an oil mixture, was penetrated to various depths into parenchyma cells of tulip bulb scale, followed by a hydraulic continuity test to determine the exact location of the tip inside target cells. The operation was conducted under a digital microscope, and the capillary tip was photographed to calculate the volume of the cell sap sucked. The cell sap sample was then directly nebulized/ionized under high-voltage conditions at the entrance of the mass spectrometer. Several sugars, amino acids, organic acids, vitamins, fatty acids, and secondary metabolites were detected. Because picoliter solutions can be accurately handled and measured, known volumes of standard solutions can be added to cell sap samples inside the capillary tip to be used as references for metabolite characterization and relative quantitation. The high precision and sensitivity of the cell pressure probe and Orbitrap mass spectrometer allow for the manipulation and analysis of both femtoliter cell sap samples and standard solutions.
Fil: Gholipour, Yousef. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Hiraoka, Kenzo. University of Yamanash; Japón
Fil: Nonami, Hiroshi. Ehime University; Japón
Materia
Ms
Esi
Ppesi
Ppesi-Ms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8153
Acceder
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spelling Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling MetabolitesGholipour, YousefErra Balsells, RosaHiraoka, KenzoNonami, HiroshiMsEsiPpesiPpesi-Mshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A modified cell pressure probe and an online Orbitrap mass spectrometer were used to sample in situ plant single cells without any additional manipulation. The cell pressure probe, a quartz capillary tip filled with an oil mixture, was penetrated to various depths into parenchyma cells of tulip bulb scale, followed by a hydraulic continuity test to determine the exact location of the tip inside target cells. The operation was conducted under a digital microscope, and the capillary tip was photographed to calculate the volume of the cell sap sucked. The cell sap sample was then directly nebulized/ionized under high-voltage conditions at the entrance of the mass spectrometer. Several sugars, amino acids, organic acids, vitamins, fatty acids, and secondary metabolites were detected. Because picoliter solutions can be accurately handled and measured, known volumes of standard solutions can be added to cell sap samples inside the capillary tip to be used as references for metabolite characterization and relative quantitation. The high precision and sensitivity of the cell pressure probe and Orbitrap mass spectrometer allow for the manipulation and analysis of both femtoliter cell sap samples and standard solutions.Fil: Gholipour, Yousef. Ehime University; JapónFil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Hiraoka, Kenzo. University of Yamanash; JapónFil: Nonami, Hiroshi. Ehime University; JapónElsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8153Gholipour, Yousef; Erra Balsells, Rosa; Hiraoka, Kenzo; Nonami, Hiroshi; Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites; Elsevier; Analytical Biochemistry; 433; 1; 1-2013; 70-780003-2697enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003269712004952info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ab.2012.10.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:00Zoai:ri.conicet.gov.ar:11336/8153instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:00.263CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites
title Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites
spellingShingle Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites
Gholipour, Yousef
Ms
Esi
Ppesi
Ppesi-Ms
title_short Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites
title_full Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites
title_fullStr Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites
title_full_unstemmed Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites
title_sort Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites
dc.creator.none.fl_str_mv Gholipour, Yousef
Erra Balsells, Rosa
Hiraoka, Kenzo
Nonami, Hiroshi
author Gholipour, Yousef
author_facet Gholipour, Yousef
Erra Balsells, Rosa
Hiraoka, Kenzo
Nonami, Hiroshi
author_role author
author2 Erra Balsells, Rosa
Hiraoka, Kenzo
Nonami, Hiroshi
author2_role author
author
author
dc.subject.none.fl_str_mv Ms
Esi
Ppesi
Ppesi-Ms
topic Ms
Esi
Ppesi
Ppesi-Ms
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A modified cell pressure probe and an online Orbitrap mass spectrometer were used to sample in situ plant single cells without any additional manipulation. The cell pressure probe, a quartz capillary tip filled with an oil mixture, was penetrated to various depths into parenchyma cells of tulip bulb scale, followed by a hydraulic continuity test to determine the exact location of the tip inside target cells. The operation was conducted under a digital microscope, and the capillary tip was photographed to calculate the volume of the cell sap sucked. The cell sap sample was then directly nebulized/ionized under high-voltage conditions at the entrance of the mass spectrometer. Several sugars, amino acids, organic acids, vitamins, fatty acids, and secondary metabolites were detected. Because picoliter solutions can be accurately handled and measured, known volumes of standard solutions can be added to cell sap samples inside the capillary tip to be used as references for metabolite characterization and relative quantitation. The high precision and sensitivity of the cell pressure probe and Orbitrap mass spectrometer allow for the manipulation and analysis of both femtoliter cell sap samples and standard solutions.
Fil: Gholipour, Yousef. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Hiraoka, Kenzo. University of Yamanash; Japón
Fil: Nonami, Hiroshi. Ehime University; Japón
description A modified cell pressure probe and an online Orbitrap mass spectrometer were used to sample in situ plant single cells without any additional manipulation. The cell pressure probe, a quartz capillary tip filled with an oil mixture, was penetrated to various depths into parenchyma cells of tulip bulb scale, followed by a hydraulic continuity test to determine the exact location of the tip inside target cells. The operation was conducted under a digital microscope, and the capillary tip was photographed to calculate the volume of the cell sap sucked. The cell sap sample was then directly nebulized/ionized under high-voltage conditions at the entrance of the mass spectrometer. Several sugars, amino acids, organic acids, vitamins, fatty acids, and secondary metabolites were detected. Because picoliter solutions can be accurately handled and measured, known volumes of standard solutions can be added to cell sap samples inside the capillary tip to be used as references for metabolite characterization and relative quantitation. The high precision and sensitivity of the cell pressure probe and Orbitrap mass spectrometer allow for the manipulation and analysis of both femtoliter cell sap samples and standard solutions.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8153
Gholipour, Yousef; Erra Balsells, Rosa; Hiraoka, Kenzo; Nonami, Hiroshi; Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites; Elsevier; Analytical Biochemistry; 433; 1; 1-2013; 70-78
0003-2697
url http://hdl.handle.net/11336/8153
identifier_str_mv Gholipour, Yousef; Erra Balsells, Rosa; Hiraoka, Kenzo; Nonami, Hiroshi; Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites; Elsevier; Analytical Biochemistry; 433; 1; 1-2013; 70-78
0003-2697
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003269712004952
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ab.2012.10.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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