Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics

Autores
Yousef Gholipour; Erra Balsells, Rosa; Nonami, Hiroshi
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Water status and metabolite content are considered as two key features in plant cell physiological phenotype. In order to profiling "in situ" living plant cell status, turgor pressure of cells located at different locations of tissues was probed with a cell pressure probe and then cell sap was sampled and its metabolite profile was generated with using nanoESI and MALDI mass spectrometry. No purification or separation was included in workflow and picoliter cell sap samples were injected directly into a nanoESI-Orbitrap mass spectrometer and/or deposited on selected matrices from organic compounds and nanoparticles for MALDI-TOF mass spectrometry analysis. Both shotgun mass spectrometry techniques could be used for detecting and quantifying metabolites in single-cell samples. Different metabolites from neutral carbohydrates to amino acids and secondary metabolites could be detected. Quantity of two major metabolites, sucrose and kestose, was also measured in several cells and sucrose concentration was co-plotted with turgor data.
Fil: Yousef Gholipour. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Nonami, Hiroshi. Ehime University; Japón
Materia
Ms
Uv-Maldi-Ms
Esi-Ms
Ppesi-Ms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/68280
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and MetabolomicsYousef GholipourErra Balsells, RosaNonami, HiroshiMsUv-Maldi-MsEsi-MsPpesi-Mshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Water status and metabolite content are considered as two key features in plant cell physiological phenotype. In order to profiling "in situ" living plant cell status, turgor pressure of cells located at different locations of tissues was probed with a cell pressure probe and then cell sap was sampled and its metabolite profile was generated with using nanoESI and MALDI mass spectrometry. No purification or separation was included in workflow and picoliter cell sap samples were injected directly into a nanoESI-Orbitrap mass spectrometer and/or deposited on selected matrices from organic compounds and nanoparticles for MALDI-TOF mass spectrometry analysis. Both shotgun mass spectrometry techniques could be used for detecting and quantifying metabolites in single-cell samples. Different metabolites from neutral carbohydrates to amino acids and secondary metabolites could be detected. Quantity of two major metabolites, sucrose and kestose, was also measured in several cells and sucrose concentration was co-plotted with turgor data.Fil: Yousef Gholipour. Ehime University; JapónFil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Nonami, Hiroshi. Ehime University; JapónInternational Association of Engineers2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68280Yousef Gholipour; Erra Balsells, Rosa; Nonami, Hiroshi; Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics; International Association of Engineers; Engineering Letters; 20; 12-2012; 294-3001816-093X1816-0948CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.engineeringletters.com/issues_v20/issue_4/index.htmlinfo:eu-repo/semantics/altIdentifier/url/http://www.engineeringletters.com/issues_v20/issue_4/EL_20_4_01.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:39Zoai:ri.conicet.gov.ar:11336/68280instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:39.311CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics
title Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics
spellingShingle Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics
Yousef Gholipour
Ms
Uv-Maldi-Ms
Esi-Ms
Ppesi-Ms
title_short Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics
title_full Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics
title_fullStr Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics
title_full_unstemmed Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics
title_sort Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics
dc.creator.none.fl_str_mv Yousef Gholipour
Erra Balsells, Rosa
Nonami, Hiroshi
author Yousef Gholipour
author_facet Yousef Gholipour
Erra Balsells, Rosa
Nonami, Hiroshi
author_role author
author2 Erra Balsells, Rosa
Nonami, Hiroshi
author2_role author
author
dc.subject.none.fl_str_mv Ms
Uv-Maldi-Ms
Esi-Ms
Ppesi-Ms
topic Ms
Uv-Maldi-Ms
Esi-Ms
Ppesi-Ms
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Water status and metabolite content are considered as two key features in plant cell physiological phenotype. In order to profiling "in situ" living plant cell status, turgor pressure of cells located at different locations of tissues was probed with a cell pressure probe and then cell sap was sampled and its metabolite profile was generated with using nanoESI and MALDI mass spectrometry. No purification or separation was included in workflow and picoliter cell sap samples were injected directly into a nanoESI-Orbitrap mass spectrometer and/or deposited on selected matrices from organic compounds and nanoparticles for MALDI-TOF mass spectrometry analysis. Both shotgun mass spectrometry techniques could be used for detecting and quantifying metabolites in single-cell samples. Different metabolites from neutral carbohydrates to amino acids and secondary metabolites could be detected. Quantity of two major metabolites, sucrose and kestose, was also measured in several cells and sucrose concentration was co-plotted with turgor data.
Fil: Yousef Gholipour. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Nonami, Hiroshi. Ehime University; Japón
description Water status and metabolite content are considered as two key features in plant cell physiological phenotype. In order to profiling "in situ" living plant cell status, turgor pressure of cells located at different locations of tissues was probed with a cell pressure probe and then cell sap was sampled and its metabolite profile was generated with using nanoESI and MALDI mass spectrometry. No purification or separation was included in workflow and picoliter cell sap samples were injected directly into a nanoESI-Orbitrap mass spectrometer and/or deposited on selected matrices from organic compounds and nanoparticles for MALDI-TOF mass spectrometry analysis. Both shotgun mass spectrometry techniques could be used for detecting and quantifying metabolites in single-cell samples. Different metabolites from neutral carbohydrates to amino acids and secondary metabolites could be detected. Quantity of two major metabolites, sucrose and kestose, was also measured in several cells and sucrose concentration was co-plotted with turgor data.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68280
Yousef Gholipour; Erra Balsells, Rosa; Nonami, Hiroshi; Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics; International Association of Engineers; Engineering Letters; 20; 12-2012; 294-300
1816-093X
1816-0948
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68280
identifier_str_mv Yousef Gholipour; Erra Balsells, Rosa; Nonami, Hiroshi; Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics; International Association of Engineers; Engineering Letters; 20; 12-2012; 294-300
1816-093X
1816-0948
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.engineeringletters.com/issues_v20/issue_4/index.html
info:eu-repo/semantics/altIdentifier/url/http://www.engineeringletters.com/issues_v20/issue_4/EL_20_4_01.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Association of Engineers
publisher.none.fl_str_mv International Association of Engineers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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