Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina

Autores
Zanini, Andrea Alejandra; Cuellar, Wilmer J.; Celli, Marcos Giovani; Luque, Andres Vicente; Medina, Ricardo Daniel; Conci, Vilma Cecilia; Di Feo, Liliana Del Valle
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus (Alphaflexiviridae). In Argentina, cassava is grown mainly in the northeast (NEA) region that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated with severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT-PCR and sequencing. In this work, the virus has been successfully purified and a double-antibody sandwich (DAS-) ELISA test has been developed from an Argentinean isolate of CsCMV to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co-infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate phylogenetic cluster was formed by isolates from the NEA region that only shared 77.1% to 80.3% nucleotide identity with the other clusters. These results indicate the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America.
Instituto de Patología Vegetal
Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cuellar, Wilmer J. International Center for Tropical Agriculture. Agrobiodiversity Research Area. Virology Laboratory; Colombia. Consortium of International Agricultural Research Centers. Research Program on Roots Tubers and Bananas; Colombia
Fil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luque, Andres Vicente. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Feo, Liliana Del Valle. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Plant pathology 67 (8) : 1814-1820. (October 2018)
Materia
Mandioca
Cassava
Manihot Esculenta
Virus de las Plantas
Plant Viruses
African Cassava Mosaic Virus
Geminivirus Latente de la Yuca Africana
Vigilancia de Enfermedades
Disease Surveillance
Cassava Common Mosaic Disease
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3652
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3652
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network_name_str INTA Digital (INTA)
spelling Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in ArgentinaZanini, Andrea AlejandraCuellar, Wilmer J.Celli, Marcos GiovaniLuque, Andres VicenteMedina, Ricardo DanielConci, Vilma CeciliaDi Feo, Liliana Del ValleMandiocaCassavaManihot EsculentaVirus de las PlantasPlant VirusesAfrican Cassava Mosaic VirusGeminivirus Latente de la Yuca AfricanaVigilancia de EnfermedadesDisease SurveillanceCassava Common Mosaic DiseaseArgentinaCassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus (Alphaflexiviridae). In Argentina, cassava is grown mainly in the northeast (NEA) region that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated with severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT-PCR and sequencing. In this work, the virus has been successfully purified and a double-antibody sandwich (DAS-) ELISA test has been developed from an Argentinean isolate of CsCMV to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co-infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate phylogenetic cluster was formed by isolates from the NEA region that only shared 77.1% to 80.3% nucleotide identity with the other clusters. These results indicate the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America.Instituto de Patología VegetalFil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cuellar, Wilmer J. International Center for Tropical Agriculture. Agrobiodiversity Research Area. Virology Laboratory; Colombia. Consortium of International Agricultural Research Centers. Research Program on Roots Tubers and Bananas; ColombiaFil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Luque, Andres Vicente. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Di Feo, Liliana Del Valle. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2018-10-19T18:03:00Z2018-10-19T18:03:00Z2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3652https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.128691365-3059https://doi.org/10.1111/ppa.12869Plant pathology 67 (8) : 1814-1820. (October 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/restrictedAccess2025-09-29T13:44:28Zoai:localhost:20.500.12123/3652instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:28.482INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina
title Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina
spellingShingle Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina
Zanini, Andrea Alejandra
Mandioca
Cassava
Manihot Esculenta
Virus de las Plantas
Plant Viruses
African Cassava Mosaic Virus
Geminivirus Latente de la Yuca Africana
Vigilancia de Enfermedades
Disease Surveillance
Cassava Common Mosaic Disease
Argentina
title_short Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina
title_full Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina
title_fullStr Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina
title_full_unstemmed Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina
title_sort Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina
dc.creator.none.fl_str_mv Zanini, Andrea Alejandra
Cuellar, Wilmer J.
Celli, Marcos Giovani
Luque, Andres Vicente
Medina, Ricardo Daniel
Conci, Vilma Cecilia
Di Feo, Liliana Del Valle
author Zanini, Andrea Alejandra
author_facet Zanini, Andrea Alejandra
Cuellar, Wilmer J.
Celli, Marcos Giovani
Luque, Andres Vicente
Medina, Ricardo Daniel
Conci, Vilma Cecilia
Di Feo, Liliana Del Valle
author_role author
author2 Cuellar, Wilmer J.
Celli, Marcos Giovani
Luque, Andres Vicente
Medina, Ricardo Daniel
Conci, Vilma Cecilia
Di Feo, Liliana Del Valle
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Mandioca
Cassava
Manihot Esculenta
Virus de las Plantas
Plant Viruses
African Cassava Mosaic Virus
Geminivirus Latente de la Yuca Africana
Vigilancia de Enfermedades
Disease Surveillance
Cassava Common Mosaic Disease
Argentina
topic Mandioca
Cassava
Manihot Esculenta
Virus de las Plantas
Plant Viruses
African Cassava Mosaic Virus
Geminivirus Latente de la Yuca Africana
Vigilancia de Enfermedades
Disease Surveillance
Cassava Common Mosaic Disease
Argentina
dc.description.none.fl_txt_mv Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus (Alphaflexiviridae). In Argentina, cassava is grown mainly in the northeast (NEA) region that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated with severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT-PCR and sequencing. In this work, the virus has been successfully purified and a double-antibody sandwich (DAS-) ELISA test has been developed from an Argentinean isolate of CsCMV to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co-infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate phylogenetic cluster was formed by isolates from the NEA region that only shared 77.1% to 80.3% nucleotide identity with the other clusters. These results indicate the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America.
Instituto de Patología Vegetal
Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cuellar, Wilmer J. International Center for Tropical Agriculture. Agrobiodiversity Research Area. Virology Laboratory; Colombia. Consortium of International Agricultural Research Centers. Research Program on Roots Tubers and Bananas; Colombia
Fil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luque, Andres Vicente. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Feo, Liliana Del Valle. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus (Alphaflexiviridae). In Argentina, cassava is grown mainly in the northeast (NEA) region that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated with severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT-PCR and sequencing. In this work, the virus has been successfully purified and a double-antibody sandwich (DAS-) ELISA test has been developed from an Argentinean isolate of CsCMV to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co-infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate phylogenetic cluster was formed by isolates from the NEA region that only shared 77.1% to 80.3% nucleotide identity with the other clusters. These results indicate the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-19T18:03:00Z
2018-10-19T18:03:00Z
2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3652
https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12869
1365-3059
https://doi.org/10.1111/ppa.12869
url http://hdl.handle.net/20.500.12123/3652
https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12869
https://doi.org/10.1111/ppa.12869
identifier_str_mv 1365-3059
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina (nation)
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Plant pathology 67 (8) : 1814-1820. (October 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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