Photo-activatable surfaces for cell migration assays

Autores
Salierno, Marcelo Javier; Garcia, Andres J.; del Campo, Aránzazu
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A simple material platform for advanced cell migration studies is presented. It relies on surfaces modified with photo-activatable adhesion peptides and two light exposure steps to independently trigger attachment and migration. The light-controlled exposure of adhesive ligands allows precise and selective control over integrin-mediated adhesion and migration events, including their dynamic variation. By using a microscope-coupled scanning laser, user-defined geometry, size and composition of the adhesion and migration spaces are possible and simply defined by the laser scanning parameters. Time lapse and high resolution microscopy are possible during the complete process. The miniaturization capabilities of this assay also allow (i) parallel or sequential migration experiments and controls on the same view field of the microscope, (ii) migration studies in confined and unconventional geometries or (iii) activation of migration spaces at subcellular resolution, all of these with minimum material and time consumption. The photo-activatable surfaces will allow studies of the dynamics of integrin-dependent migration processes with unprecedented flexibility, multiplexing, reproducibility, and spatiotemporal resolution.
Fil: Salierno, Marcelo Javier. Max Planck Institut für Polymerforschung Ackermannweg; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia, Andres J.. Georgia Institute Of Techology; Estados Unidos
Fil: del Campo, Aránzazu. Max Planck Institut für Polymerforschung Ackermannweg; Alemania
Materia
Cell Migration
Cyclcrgd
Photoactivatable Molecules
Integrins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15662
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/15662
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Photo-activatable surfaces for cell migration assaysSalierno, Marcelo JavierGarcia, Andres J.del Campo, AránzazuCell MigrationCyclcrgdPhotoactivatable MoleculesIntegrinshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A simple material platform for advanced cell migration studies is presented. It relies on surfaces modified with photo-activatable adhesion peptides and two light exposure steps to independently trigger attachment and migration. The light-controlled exposure of adhesive ligands allows precise and selective control over integrin-mediated adhesion and migration events, including their dynamic variation. By using a microscope-coupled scanning laser, user-defined geometry, size and composition of the adhesion and migration spaces are possible and simply defined by the laser scanning parameters. Time lapse and high resolution microscopy are possible during the complete process. The miniaturization capabilities of this assay also allow (i) parallel or sequential migration experiments and controls on the same view field of the microscope, (ii) migration studies in confined and unconventional geometries or (iii) activation of migration spaces at subcellular resolution, all of these with minimum material and time consumption. The photo-activatable surfaces will allow studies of the dynamics of integrin-dependent migration processes with unprecedented flexibility, multiplexing, reproducibility, and spatiotemporal resolution.Fil: Salierno, Marcelo Javier. Max Planck Institut für Polymerforschung Ackermannweg; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia, Andres J.. Georgia Institute Of Techology; Estados UnidosFil: del Campo, Aránzazu. Max Planck Institut für Polymerforschung Ackermannweg; AlemaniaWiley Vch Verlag2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15662Salierno, Marcelo Javier; Garcia, Andres J.; del Campo, Aránzazu; Photo-activatable surfaces for cell migration assays; Wiley Vch Verlag; Advanced Functional Materials; 23; 48; 12-2013; 5974-59801616-301Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/adfm.201300902info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/adfm.201300902/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:17:42Zoai:ri.conicet.gov.ar:11336/15662instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:17:42.939CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Photo-activatable surfaces for cell migration assays
title Photo-activatable surfaces for cell migration assays
spellingShingle Photo-activatable surfaces for cell migration assays
Salierno, Marcelo Javier
Cell Migration
Cyclcrgd
Photoactivatable Molecules
Integrins
title_short Photo-activatable surfaces for cell migration assays
title_full Photo-activatable surfaces for cell migration assays
title_fullStr Photo-activatable surfaces for cell migration assays
title_full_unstemmed Photo-activatable surfaces for cell migration assays
title_sort Photo-activatable surfaces for cell migration assays
dc.creator.none.fl_str_mv Salierno, Marcelo Javier
Garcia, Andres J.
del Campo, Aránzazu
author Salierno, Marcelo Javier
author_facet Salierno, Marcelo Javier
Garcia, Andres J.
del Campo, Aránzazu
author_role author
author2 Garcia, Andres J.
del Campo, Aránzazu
author2_role author
author
dc.subject.none.fl_str_mv Cell Migration
Cyclcrgd
Photoactivatable Molecules
Integrins
topic Cell Migration
Cyclcrgd
Photoactivatable Molecules
Integrins
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A simple material platform for advanced cell migration studies is presented. It relies on surfaces modified with photo-activatable adhesion peptides and two light exposure steps to independently trigger attachment and migration. The light-controlled exposure of adhesive ligands allows precise and selective control over integrin-mediated adhesion and migration events, including their dynamic variation. By using a microscope-coupled scanning laser, user-defined geometry, size and composition of the adhesion and migration spaces are possible and simply defined by the laser scanning parameters. Time lapse and high resolution microscopy are possible during the complete process. The miniaturization capabilities of this assay also allow (i) parallel or sequential migration experiments and controls on the same view field of the microscope, (ii) migration studies in confined and unconventional geometries or (iii) activation of migration spaces at subcellular resolution, all of these with minimum material and time consumption. The photo-activatable surfaces will allow studies of the dynamics of integrin-dependent migration processes with unprecedented flexibility, multiplexing, reproducibility, and spatiotemporal resolution.
Fil: Salierno, Marcelo Javier. Max Planck Institut für Polymerforschung Ackermannweg; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia, Andres J.. Georgia Institute Of Techology; Estados Unidos
Fil: del Campo, Aránzazu. Max Planck Institut für Polymerforschung Ackermannweg; Alemania
description A simple material platform for advanced cell migration studies is presented. It relies on surfaces modified with photo-activatable adhesion peptides and two light exposure steps to independently trigger attachment and migration. The light-controlled exposure of adhesive ligands allows precise and selective control over integrin-mediated adhesion and migration events, including their dynamic variation. By using a microscope-coupled scanning laser, user-defined geometry, size and composition of the adhesion and migration spaces are possible and simply defined by the laser scanning parameters. Time lapse and high resolution microscopy are possible during the complete process. The miniaturization capabilities of this assay also allow (i) parallel or sequential migration experiments and controls on the same view field of the microscope, (ii) migration studies in confined and unconventional geometries or (iii) activation of migration spaces at subcellular resolution, all of these with minimum material and time consumption. The photo-activatable surfaces will allow studies of the dynamics of integrin-dependent migration processes with unprecedented flexibility, multiplexing, reproducibility, and spatiotemporal resolution.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15662
Salierno, Marcelo Javier; Garcia, Andres J.; del Campo, Aránzazu; Photo-activatable surfaces for cell migration assays; Wiley Vch Verlag; Advanced Functional Materials; 23; 48; 12-2013; 5974-5980
1616-301X
url http://hdl.handle.net/11336/15662
identifier_str_mv Salierno, Marcelo Javier; Garcia, Andres J.; del Campo, Aránzazu; Photo-activatable surfaces for cell migration assays; Wiley Vch Verlag; Advanced Functional Materials; 23; 48; 12-2013; 5974-5980
1616-301X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/adfm.201300902
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/adfm.201300902/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Vch Verlag
publisher.none.fl_str_mv Wiley Vch Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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