Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding

Autores
Iturri, Jagoba; García Fernández, Luis; Reuning, Ute; García, Andrés J.; del Campo, Aránzazu; Salierno, Marcelo Javier
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Quartz Crystal Microbalance with dissipation (QCM-D) technique was applied to monitor and quantify integrin-RGD recognition during the early stages of cell adhesion. Using QCM-D crystals modified with a photo-activatable RGD peptide, the time point of presentation of adhesive ligand at the surface of the QCM-D crystal could be accurately controlled. This allowed temporal resolution of early integrin-RGD binding and the subsequent cell spreading process, and their separate detection by QCM-D. The specificity of the integrin-RGD binding event was corroborated by performing the experiments in the presence of soluble cyclicRGD as a competitor, and cytochalasin D as inhibitor of cell spreading. Larger frequency change in the QCM-D signal was observed for cells with larger spread area, and for cells overexpressing integrin avb3 upon stable transfection. This strategy enables quantification of integrin activity which, in turn, may allow discrimination among different cell types displaying distinct integrin subtypes and expression levels thereof. On the basis of these findings, we believe the strategy can be extended to other photoactivatable ligands to characterize cell membrane receptors activity, a relevant issue for cancer diagnosis (and prognosis) as other several pathologies.
Fil: Iturri, Jagoba. Max Planck Institute for Polymer Research; Alemania
Fil: García Fernández, Luis. Max Planck Institute for Polymer Research; Alemania
Fil: Reuning, Ute. Technische Universitat Munchen; Alemania
Fil: García, Andrés J.. Georgia Institute Of Techology; Estados Unidos
Fil: del Campo, Aránzazu. Max Planck Institute for Polymer Research; Alemania
Fil: Salierno, Marcelo Javier. Max Planck Institute for Polymer Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Integrins
Cancer screening
Lab-on-a-chip
Cell adhesion
QCM-D
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16694
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16694
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin bindingIturri, JagobaGarcía Fernández, LuisReuning, UteGarcía, Andrés J.del Campo, AránzazuSalierno, Marcelo JavierIntegrinsCancer screeningLab-on-a-chipCell adhesionQCM-Dhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Quartz Crystal Microbalance with dissipation (QCM-D) technique was applied to monitor and quantify integrin-RGD recognition during the early stages of cell adhesion. Using QCM-D crystals modified with a photo-activatable RGD peptide, the time point of presentation of adhesive ligand at the surface of the QCM-D crystal could be accurately controlled. This allowed temporal resolution of early integrin-RGD binding and the subsequent cell spreading process, and their separate detection by QCM-D. The specificity of the integrin-RGD binding event was corroborated by performing the experiments in the presence of soluble cyclicRGD as a competitor, and cytochalasin D as inhibitor of cell spreading. Larger frequency change in the QCM-D signal was observed for cells with larger spread area, and for cells overexpressing integrin avb3 upon stable transfection. This strategy enables quantification of integrin activity which, in turn, may allow discrimination among different cell types displaying distinct integrin subtypes and expression levels thereof. On the basis of these findings, we believe the strategy can be extended to other photoactivatable ligands to characterize cell membrane receptors activity, a relevant issue for cancer diagnosis (and prognosis) as other several pathologies.Fil: Iturri, Jagoba. Max Planck Institute for Polymer Research; AlemaniaFil: García Fernández, Luis. Max Planck Institute for Polymer Research; AlemaniaFil: Reuning, Ute. Technische Universitat Munchen; AlemaniaFil: García, Andrés J.. Georgia Institute Of Techology; Estados UnidosFil: del Campo, Aránzazu. Max Planck Institute for Polymer Research; AlemaniaFil: Salierno, Marcelo Javier. Max Planck Institute for Polymer Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaNature Publishing Group2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16694Iturri, Jagoba; García Fernández, Luis; Reuning, Ute; García, Andrés J.; del Campo, Aránzazu; et al.; Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding; Nature Publishing Group; Scientific Reports; 5; 3-2015; 1-8; 95332045-2322enginfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep09533info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep09533info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379501/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:37:30Zoai:ri.conicet.gov.ar:11336/16694instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:37:31.104CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding
title Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding
spellingShingle Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding
Iturri, Jagoba
Integrins
Cancer screening
Lab-on-a-chip
Cell adhesion
QCM-D
title_short Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding
title_full Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding
title_fullStr Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding
title_full_unstemmed Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding
title_sort Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding
dc.creator.none.fl_str_mv Iturri, Jagoba
García Fernández, Luis
Reuning, Ute
García, Andrés J.
del Campo, Aránzazu
Salierno, Marcelo Javier
author Iturri, Jagoba
author_facet Iturri, Jagoba
García Fernández, Luis
Reuning, Ute
García, Andrés J.
del Campo, Aránzazu
Salierno, Marcelo Javier
author_role author
author2 García Fernández, Luis
Reuning, Ute
García, Andrés J.
del Campo, Aránzazu
Salierno, Marcelo Javier
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Integrins
Cancer screening
Lab-on-a-chip
Cell adhesion
QCM-D
topic Integrins
Cancer screening
Lab-on-a-chip
Cell adhesion
QCM-D
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Quartz Crystal Microbalance with dissipation (QCM-D) technique was applied to monitor and quantify integrin-RGD recognition during the early stages of cell adhesion. Using QCM-D crystals modified with a photo-activatable RGD peptide, the time point of presentation of adhesive ligand at the surface of the QCM-D crystal could be accurately controlled. This allowed temporal resolution of early integrin-RGD binding and the subsequent cell spreading process, and their separate detection by QCM-D. The specificity of the integrin-RGD binding event was corroborated by performing the experiments in the presence of soluble cyclicRGD as a competitor, and cytochalasin D as inhibitor of cell spreading. Larger frequency change in the QCM-D signal was observed for cells with larger spread area, and for cells overexpressing integrin avb3 upon stable transfection. This strategy enables quantification of integrin activity which, in turn, may allow discrimination among different cell types displaying distinct integrin subtypes and expression levels thereof. On the basis of these findings, we believe the strategy can be extended to other photoactivatable ligands to characterize cell membrane receptors activity, a relevant issue for cancer diagnosis (and prognosis) as other several pathologies.
Fil: Iturri, Jagoba. Max Planck Institute for Polymer Research; Alemania
Fil: García Fernández, Luis. Max Planck Institute for Polymer Research; Alemania
Fil: Reuning, Ute. Technische Universitat Munchen; Alemania
Fil: García, Andrés J.. Georgia Institute Of Techology; Estados Unidos
Fil: del Campo, Aránzazu. Max Planck Institute for Polymer Research; Alemania
Fil: Salierno, Marcelo Javier. Max Planck Institute for Polymer Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The Quartz Crystal Microbalance with dissipation (QCM-D) technique was applied to monitor and quantify integrin-RGD recognition during the early stages of cell adhesion. Using QCM-D crystals modified with a photo-activatable RGD peptide, the time point of presentation of adhesive ligand at the surface of the QCM-D crystal could be accurately controlled. This allowed temporal resolution of early integrin-RGD binding and the subsequent cell spreading process, and their separate detection by QCM-D. The specificity of the integrin-RGD binding event was corroborated by performing the experiments in the presence of soluble cyclicRGD as a competitor, and cytochalasin D as inhibitor of cell spreading. Larger frequency change in the QCM-D signal was observed for cells with larger spread area, and for cells overexpressing integrin avb3 upon stable transfection. This strategy enables quantification of integrin activity which, in turn, may allow discrimination among different cell types displaying distinct integrin subtypes and expression levels thereof. On the basis of these findings, we believe the strategy can be extended to other photoactivatable ligands to characterize cell membrane receptors activity, a relevant issue for cancer diagnosis (and prognosis) as other several pathologies.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16694
Iturri, Jagoba; García Fernández, Luis; Reuning, Ute; García, Andrés J.; del Campo, Aránzazu; et al.; Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding; Nature Publishing Group; Scientific Reports; 5; 3-2015; 1-8; 9533
2045-2322
url http://hdl.handle.net/11336/16694
identifier_str_mv Iturri, Jagoba; García Fernández, Luis; Reuning, Ute; García, Andrés J.; del Campo, Aránzazu; et al.; Synchronized cell attachment triggered by photo-activatable adhesive ligands allows QCM-based detection of early integrin binding; Nature Publishing Group; Scientific Reports; 5; 3-2015; 1-8; 9533
2045-2322
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/srep09533
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep09533
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379501/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083495938490368
score 12.891075

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