Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers

Autores
Salierno, Marcelo Javier; García Fernández, Lui; Carabelos, Noelia; Kiefer, Karin; García, Andrés J.; Campo, Aránzazu del
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cell detachment and migration from the endothelium occurs during vasculogenesis and also in pathological states. Here, we use a novel approach to trigger single cell release from an endothelial monolayer by in-situ opening of adhesive, fibril-like environment using light-responsive ligands and scanning lasers. Cell escapes from the monolayer were observed on the fibril-like adhesive tracks with 3-15 μm width. The frequency of endothelial cell escapes increased monotonically with the fibril width and with the density of the light-activated adhesive ligand. Interestingly, treatment with VEGF induced cohesiveness within the cell layer, preventing cell leaks. When migrating through the tracks, cells presented body lateral reduction and nuclear deformation imposed by the line width and dependent on myosin contractility. Cell migration mode changed from mesenchymal to amoeboid-like when the adhesive tracks narrowed (≤5 μm). Moreover, cell nucleus was shrunk showing packed DNA on lines narrower than the nuclear dimensions in a mechanisms intimately associated with the stress fibers. This platform allows the detailed study of escapes and migratory transitions of cohesive cells, which are relevant processes in development and during diseases such as organ fibrosis and carcinomas.
Fil: Salierno, Marcelo Javier. Institut Max Planck For Polymerforschung,; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: García Fernández, Lui. Institut Max Planck For Polymerforschung,; Alemania
Fil: Carabelos, Noelia. Institut Max Planck For Polymerforschung,; Alemania. Universidad de Buenos Aires; Argentina
Fil: Kiefer, Karin. Leibniz-institut Fur Neue Materialien GMBH; Alemania
Fil: García, Andrés J.. Georgia Institute Of Techology; Estados Unidos. Instituto Tecnológico de Georgia; Estados Unidos
Fil: Campo, Aránzazu del. Institut Max Planck For Polymerforschung,; Alemania. Leibniz-institut Fur Neue Materialien GMBH; Alemania. Universitat Saarland; Alemania
Materia
Cell Migration
Cyclic Rgd
Endothelial Transition
Photoactive Materials
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17533
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/17533
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayersSalierno, Marcelo JavierGarcía Fernández, LuiCarabelos, NoeliaKiefer, KarinGarcía, Andrés J.Campo, Aránzazu delCell MigrationCyclic RgdEndothelial TransitionPhotoactive Materialshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Cell detachment and migration from the endothelium occurs during vasculogenesis and also in pathological states. Here, we use a novel approach to trigger single cell release from an endothelial monolayer by in-situ opening of adhesive, fibril-like environment using light-responsive ligands and scanning lasers. Cell escapes from the monolayer were observed on the fibril-like adhesive tracks with 3-15 μm width. The frequency of endothelial cell escapes increased monotonically with the fibril width and with the density of the light-activated adhesive ligand. Interestingly, treatment with VEGF induced cohesiveness within the cell layer, preventing cell leaks. When migrating through the tracks, cells presented body lateral reduction and nuclear deformation imposed by the line width and dependent on myosin contractility. Cell migration mode changed from mesenchymal to amoeboid-like when the adhesive tracks narrowed (≤5 μm). Moreover, cell nucleus was shrunk showing packed DNA on lines narrower than the nuclear dimensions in a mechanisms intimately associated with the stress fibers. This platform allows the detailed study of escapes and migratory transitions of cohesive cells, which are relevant processes in development and during diseases such as organ fibrosis and carcinomas.Fil: Salierno, Marcelo Javier. Institut Max Planck For Polymerforschung,; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: García Fernández, Lui. Institut Max Planck For Polymerforschung,; AlemaniaFil: Carabelos, Noelia. Institut Max Planck For Polymerforschung,; Alemania. Universidad de Buenos Aires; ArgentinaFil: Kiefer, Karin. Leibniz-institut Fur Neue Materialien GMBH; AlemaniaFil: García, Andrés J.. Georgia Institute Of Techology; Estados Unidos. Instituto Tecnológico de Georgia; Estados UnidosFil: Campo, Aránzazu del. Institut Max Planck For Polymerforschung,; Alemania. Leibniz-institut Fur Neue Materialien GMBH; Alemania. Universitat Saarland; AlemaniaElsevier2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17533Salierno, Marcelo Javier; García Fernández, Lui; Carabelos, Noelia; Kiefer, Karin; García, Andrés J.; et al.; Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers; Elsevier; Biomaterials; 82; 12-2015; 113-1230142-9612enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biomaterials.2015.12.001info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/26757258info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:19Zoai:ri.conicet.gov.ar:11336/17533instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:20.052CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers
title Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers
spellingShingle Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers
Salierno, Marcelo Javier
Cell Migration
Cyclic Rgd
Endothelial Transition
Photoactive Materials
title_short Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers
title_full Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers
title_fullStr Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers
title_full_unstemmed Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers
title_sort Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers
dc.creator.none.fl_str_mv Salierno, Marcelo Javier
García Fernández, Lui
Carabelos, Noelia
Kiefer, Karin
García, Andrés J.
Campo, Aránzazu del
author Salierno, Marcelo Javier
author_facet Salierno, Marcelo Javier
García Fernández, Lui
Carabelos, Noelia
Kiefer, Karin
García, Andrés J.
Campo, Aránzazu del
author_role author
author2 García Fernández, Lui
Carabelos, Noelia
Kiefer, Karin
García, Andrés J.
Campo, Aránzazu del
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cell Migration
Cyclic Rgd
Endothelial Transition
Photoactive Materials
topic Cell Migration
Cyclic Rgd
Endothelial Transition
Photoactive Materials
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cell detachment and migration from the endothelium occurs during vasculogenesis and also in pathological states. Here, we use a novel approach to trigger single cell release from an endothelial monolayer by in-situ opening of adhesive, fibril-like environment using light-responsive ligands and scanning lasers. Cell escapes from the monolayer were observed on the fibril-like adhesive tracks with 3-15 μm width. The frequency of endothelial cell escapes increased monotonically with the fibril width and with the density of the light-activated adhesive ligand. Interestingly, treatment with VEGF induced cohesiveness within the cell layer, preventing cell leaks. When migrating through the tracks, cells presented body lateral reduction and nuclear deformation imposed by the line width and dependent on myosin contractility. Cell migration mode changed from mesenchymal to amoeboid-like when the adhesive tracks narrowed (≤5 μm). Moreover, cell nucleus was shrunk showing packed DNA on lines narrower than the nuclear dimensions in a mechanisms intimately associated with the stress fibers. This platform allows the detailed study of escapes and migratory transitions of cohesive cells, which are relevant processes in development and during diseases such as organ fibrosis and carcinomas.
Fil: Salierno, Marcelo Javier. Institut Max Planck For Polymerforschung,; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: García Fernández, Lui. Institut Max Planck For Polymerforschung,; Alemania
Fil: Carabelos, Noelia. Institut Max Planck For Polymerforschung,; Alemania. Universidad de Buenos Aires; Argentina
Fil: Kiefer, Karin. Leibniz-institut Fur Neue Materialien GMBH; Alemania
Fil: García, Andrés J.. Georgia Institute Of Techology; Estados Unidos. Instituto Tecnológico de Georgia; Estados Unidos
Fil: Campo, Aránzazu del. Institut Max Planck For Polymerforschung,; Alemania. Leibniz-institut Fur Neue Materialien GMBH; Alemania. Universitat Saarland; Alemania
description Cell detachment and migration from the endothelium occurs during vasculogenesis and also in pathological states. Here, we use a novel approach to trigger single cell release from an endothelial monolayer by in-situ opening of adhesive, fibril-like environment using light-responsive ligands and scanning lasers. Cell escapes from the monolayer were observed on the fibril-like adhesive tracks with 3-15 μm width. The frequency of endothelial cell escapes increased monotonically with the fibril width and with the density of the light-activated adhesive ligand. Interestingly, treatment with VEGF induced cohesiveness within the cell layer, preventing cell leaks. When migrating through the tracks, cells presented body lateral reduction and nuclear deformation imposed by the line width and dependent on myosin contractility. Cell migration mode changed from mesenchymal to amoeboid-like when the adhesive tracks narrowed (≤5 μm). Moreover, cell nucleus was shrunk showing packed DNA on lines narrower than the nuclear dimensions in a mechanisms intimately associated with the stress fibers. This platform allows the detailed study of escapes and migratory transitions of cohesive cells, which are relevant processes in development and during diseases such as organ fibrosis and carcinomas.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17533
Salierno, Marcelo Javier; García Fernández, Lui; Carabelos, Noelia; Kiefer, Karin; García, Andrés J.; et al.; Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers; Elsevier; Biomaterials; 82; 12-2015; 113-123
0142-9612
url http://hdl.handle.net/11336/17533
identifier_str_mv Salierno, Marcelo Javier; García Fernández, Lui; Carabelos, Noelia; Kiefer, Karin; García, Andrés J.; et al.; Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers; Elsevier; Biomaterials; 82; 12-2015; 113-123
0142-9612
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biomaterials.2015.12.001
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/26757258
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980391855587328
score 12.993085

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