Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods

Autores
de Matos, Fernando Eustáquio; Santos, Tizá Teles; Burns, Patricia Graciela; Reinheimer, Jorge Alberto; Vinderola, Celso Gabriel; Trindade, Carmen Sílvia Fávaro
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Desirable characteristics for probiotic microorganisms were evaluated in Lactobacillus rhamnosus 64 and Lactobacillus paracasei LP11. Both strains were tested for resistance to lysozyme and simulated gastrointestinal fluids, determination of cell wall hydrophobicity, evaluation of antibiotic susceptibility, antagonist activity against pathogens and the ability to use prebiotics. The safety determination was performed by an in vivo test, where the occurrence of bacterial translocation to liver was evaluated after daily oral administration of the strains for 10 days to mice. All results were promising, with the exception of resistance to simulated gastrointestinal fluids, that was unsatisfactory, with a decrease in viable cell concentrations of up to 5.08 log CFU/mL. The hydrophobicity profile of the strains ranged between 8.47 and 19.19%. Both showed satisfactiry resistance to lysozyme, with survival rates above 80%, strains were able to antagonize Escherichia coli V517, Salmonella enteritidis OMS-Ca, Staphylococcus aureus 76 and Listeria monocytogenes ATCC 15313, and displayed satisfactory use of lactulose, inulin and P95 raftilose as prebiotics. No bacterial translocation was observed after the administration of the strains to mice. The results obtained herein justify the use of L. rhamnosus 64 and L. paracasei LP11 in new investigations for the future application of these microorganisms as probiotics.
Fil: de Matos, Fernando Eustáquio. Universidade de Sao Paulo; Brasil. Universidade Federal de Juiz de Fora; Brasil
Fil: Santos, Tizá Teles. Universidade Estadual de Santa Cruz; Brasil
Fil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Trindade, Carmen Sílvia Fávaro. Universidade de Sao Paulo; Brasil
Materia
HYDROPHOBICITY
LACTOBACILLI
PREBIOTICS
RESISTANCE TO GASTROINTESTINAL FLUIDS
SAFETY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/117088
Acceder
id CONICETDig_a9224175d12efcae91d2061cedc2d587
oai_identifier_str oai:ri.conicet.gov.ar:11336/117088
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foodsde Matos, Fernando EustáquioSantos, Tizá TelesBurns, Patricia GracielaReinheimer, Jorge AlbertoVinderola, Celso GabrielTrindade, Carmen Sílvia FávaroHYDROPHOBICITYLACTOBACILLIPREBIOTICSRESISTANCE TO GASTROINTESTINAL FLUIDSSAFETYhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Desirable characteristics for probiotic microorganisms were evaluated in Lactobacillus rhamnosus 64 and Lactobacillus paracasei LP11. Both strains were tested for resistance to lysozyme and simulated gastrointestinal fluids, determination of cell wall hydrophobicity, evaluation of antibiotic susceptibility, antagonist activity against pathogens and the ability to use prebiotics. The safety determination was performed by an in vivo test, where the occurrence of bacterial translocation to liver was evaluated after daily oral administration of the strains for 10 days to mice. All results were promising, with the exception of resistance to simulated gastrointestinal fluids, that was unsatisfactory, with a decrease in viable cell concentrations of up to 5.08 log CFU/mL. The hydrophobicity profile of the strains ranged between 8.47 and 19.19%. Both showed satisfactiry resistance to lysozyme, with survival rates above 80%, strains were able to antagonize Escherichia coli V517, Salmonella enteritidis OMS-Ca, Staphylococcus aureus 76 and Listeria monocytogenes ATCC 15313, and displayed satisfactory use of lactulose, inulin and P95 raftilose as prebiotics. No bacterial translocation was observed after the administration of the strains to mice. The results obtained herein justify the use of L. rhamnosus 64 and L. paracasei LP11 in new investigations for the future application of these microorganisms as probiotics.Fil: de Matos, Fernando Eustáquio. Universidade de Sao Paulo; Brasil. Universidade Federal de Juiz de Fora; BrasilFil: Santos, Tizá Teles. Universidade Estadual de Santa Cruz; BrasilFil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Trindade, Carmen Sílvia Fávaro. Universidade de Sao Paulo; BrasilSlovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/117088de Matos, Fernando Eustáquio; Santos, Tizá Teles; Burns, Patricia Graciela; Reinheimer, Jorge Alberto; Vinderola, Celso Gabriel; et al.; Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods; Slovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences; Journal of Microbiology, Biotechnology and Food Sciences; 9; 6; 6-2020; 1126-11331338-5178CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jmbfs.org/wp-content/uploads/2020/05/jmbfs_1097_Eust%C3%A1quio-de-Matos.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.15414/jmbfs.2020.9.6.1126-1133info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:53:24Zoai:ri.conicet.gov.ar:11336/117088instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:53:24.353CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods
title Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods
spellingShingle Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods
de Matos, Fernando Eustáquio
HYDROPHOBICITY
LACTOBACILLI
PREBIOTICS
RESISTANCE TO GASTROINTESTINAL FLUIDS
SAFETY
title_short Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods
title_full Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods
title_fullStr Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods
title_full_unstemmed Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods
title_sort Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods
dc.creator.none.fl_str_mv de Matos, Fernando Eustáquio
Santos, Tizá Teles
Burns, Patricia Graciela
Reinheimer, Jorge Alberto
Vinderola, Celso Gabriel
Trindade, Carmen Sílvia Fávaro
author de Matos, Fernando Eustáquio
author_facet de Matos, Fernando Eustáquio
Santos, Tizá Teles
Burns, Patricia Graciela
Reinheimer, Jorge Alberto
Vinderola, Celso Gabriel
Trindade, Carmen Sílvia Fávaro
author_role author
author2 Santos, Tizá Teles
Burns, Patricia Graciela
Reinheimer, Jorge Alberto
Vinderola, Celso Gabriel
Trindade, Carmen Sílvia Fávaro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv HYDROPHOBICITY
LACTOBACILLI
PREBIOTICS
RESISTANCE TO GASTROINTESTINAL FLUIDS
SAFETY
topic HYDROPHOBICITY
LACTOBACILLI
PREBIOTICS
RESISTANCE TO GASTROINTESTINAL FLUIDS
SAFETY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Desirable characteristics for probiotic microorganisms were evaluated in Lactobacillus rhamnosus 64 and Lactobacillus paracasei LP11. Both strains were tested for resistance to lysozyme and simulated gastrointestinal fluids, determination of cell wall hydrophobicity, evaluation of antibiotic susceptibility, antagonist activity against pathogens and the ability to use prebiotics. The safety determination was performed by an in vivo test, where the occurrence of bacterial translocation to liver was evaluated after daily oral administration of the strains for 10 days to mice. All results were promising, with the exception of resistance to simulated gastrointestinal fluids, that was unsatisfactory, with a decrease in viable cell concentrations of up to 5.08 log CFU/mL. The hydrophobicity profile of the strains ranged between 8.47 and 19.19%. Both showed satisfactiry resistance to lysozyme, with survival rates above 80%, strains were able to antagonize Escherichia coli V517, Salmonella enteritidis OMS-Ca, Staphylococcus aureus 76 and Listeria monocytogenes ATCC 15313, and displayed satisfactory use of lactulose, inulin and P95 raftilose as prebiotics. No bacterial translocation was observed after the administration of the strains to mice. The results obtained herein justify the use of L. rhamnosus 64 and L. paracasei LP11 in new investigations for the future application of these microorganisms as probiotics.
Fil: de Matos, Fernando Eustáquio. Universidade de Sao Paulo; Brasil. Universidade Federal de Juiz de Fora; Brasil
Fil: Santos, Tizá Teles. Universidade Estadual de Santa Cruz; Brasil
Fil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Trindade, Carmen Sílvia Fávaro. Universidade de Sao Paulo; Brasil
description Desirable characteristics for probiotic microorganisms were evaluated in Lactobacillus rhamnosus 64 and Lactobacillus paracasei LP11. Both strains were tested for resistance to lysozyme and simulated gastrointestinal fluids, determination of cell wall hydrophobicity, evaluation of antibiotic susceptibility, antagonist activity against pathogens and the ability to use prebiotics. The safety determination was performed by an in vivo test, where the occurrence of bacterial translocation to liver was evaluated after daily oral administration of the strains for 10 days to mice. All results were promising, with the exception of resistance to simulated gastrointestinal fluids, that was unsatisfactory, with a decrease in viable cell concentrations of up to 5.08 log CFU/mL. The hydrophobicity profile of the strains ranged between 8.47 and 19.19%. Both showed satisfactiry resistance to lysozyme, with survival rates above 80%, strains were able to antagonize Escherichia coli V517, Salmonella enteritidis OMS-Ca, Staphylococcus aureus 76 and Listeria monocytogenes ATCC 15313, and displayed satisfactory use of lactulose, inulin and P95 raftilose as prebiotics. No bacterial translocation was observed after the administration of the strains to mice. The results obtained herein justify the use of L. rhamnosus 64 and L. paracasei LP11 in new investigations for the future application of these microorganisms as probiotics.
publishDate 2020
dc.date.none.fl_str_mv 2020-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/117088
de Matos, Fernando Eustáquio; Santos, Tizá Teles; Burns, Patricia Graciela; Reinheimer, Jorge Alberto; Vinderola, Celso Gabriel; et al.; Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods; Slovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences; Journal of Microbiology, Biotechnology and Food Sciences; 9; 6; 6-2020; 1126-1133
1338-5178
CONICET Digital
CONICET
url http://hdl.handle.net/11336/117088
identifier_str_mv de Matos, Fernando Eustáquio; Santos, Tizá Teles; Burns, Patricia Graciela; Reinheimer, Jorge Alberto; Vinderola, Celso Gabriel; et al.; Evaluation of lactobacillus paracasei LP11 and lactobacillus rhamnosus 64 potential as candidates for use as probiotics in functional foods; Slovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences; Journal of Microbiology, Biotechnology and Food Sciences; 9; 6; 6-2020; 1126-1133
1338-5178
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jmbfs.org/wp-content/uploads/2020/05/jmbfs_1097_Eust%C3%A1quio-de-Matos.pdf
info:eu-repo/semantics/altIdentifier/doi/10.15414/jmbfs.2020.9.6.1126-1133
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Slovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences
publisher.none.fl_str_mv Slovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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