Expression of heme oxygenase-1 in human gliomas

Autores
Gandini, Norberto Ariel; Salomón, Débora; Fermento, María Eugenia; Zenklusen, Jean C.; Robles, Ana; Curino, Alejandro Carlos; Facchinetti, María Marta
Año de publicación
2009
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The vast majority of experiments performed in different tumor cells indicate that HO-1 is a potent cytoprotective and antiapoptotic enzyme wich improves survival of cancer cells subjected to different kinds of therapy. In glioma tumours HO-1 has been shown to be upregulated as compared with normal brain, although the significance of this upregulation is not clear. Therefore, the aim of our study was to perform a wide screening of heme oxygenase-1 (HO1) expression in glioma by using tissue microarrays (TMA) containing  astrocytomas (18), oligodendrogliomas (29), mixed tumors (12), glioblastoma multiforme (GBM. 57) and normal brain (18) and to correlate protein expression with patient clinic pathological data. HO-1 showed cytoplasmic localization, although a few cells also presented nuclear staining. Nuclear HO-1 was confirmed in T98 and U87 cells. HO-1 was positive in 51% of GBM, 62% astrocytomas, 61%oligodendrogliomas, 42% of mixed and 22% of normal tissue. We found differences in HO-1 positivity rates between normal brain and oligodendrogliomas (p=0.012) and astrocytomas (p=0.027). Comparison by the Kaplan Meier method revealed a sgnificant decreasein overall survival of astrocytoma patients with HO-1 positivity (p=0.03, log rank test). We also performed RT-PCR for mRNA quantification, although no significant differences were observed between normal (n=5) and malignant (n=5). In conclusion, our results corroborate higher frecuency of HO-1 protein expression in gliomas than in normal brain and show a correlation of HO-1 expression with patient survival.
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salomón, Débora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Zenklusen, Jean C.. Public Health Service. National Institute Of Health; Estados Unidos
Fil: Robles, Ana. Public Health Service. National Institute Of Health; Estados Unidos
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Facchinetti, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Heme Oxygenase in Biology & Medicine: 6Th International Congress on Heme oxygenases
Miami Beach
Estados Unidos
Comité internacional del 6Th International Congress on Heme oxygenases
Materia
HEMEOXYGENASE-1
GLIOMAS
CANCER
EXPRESSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242796
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/242796
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of heme oxygenase-1 in human gliomasGandini, Norberto ArielSalomón, DéboraFermento, María EugeniaZenklusen, Jean C.Robles, AnaCurino, Alejandro CarlosFacchinetti, María MartaHEMEOXYGENASE-1GLIOMASCANCEREXPRESSIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The vast majority of experiments performed in different tumor cells indicate that HO-1 is a potent cytoprotective and antiapoptotic enzyme wich improves survival of cancer cells subjected to different kinds of therapy. In glioma tumours HO-1 has been shown to be upregulated as compared with normal brain, although the significance of this upregulation is not clear. Therefore, the aim of our study was to perform a wide screening of heme oxygenase-1 (HO1) expression in glioma by using tissue microarrays (TMA) containing  astrocytomas (18), oligodendrogliomas (29), mixed tumors (12), glioblastoma multiforme (GBM. 57) and normal brain (18) and to correlate protein expression with patient clinic pathological data. HO-1 showed cytoplasmic localization, although a few cells also presented nuclear staining. Nuclear HO-1 was confirmed in T98 and U87 cells. HO-1 was positive in 51% of GBM, 62% astrocytomas, 61%oligodendrogliomas, 42% of mixed and 22% of normal tissue. We found differences in HO-1 positivity rates between normal brain and oligodendrogliomas (p=0.012) and astrocytomas (p=0.027). Comparison by the Kaplan Meier method revealed a sgnificant decreasein overall survival of astrocytoma patients with HO-1 positivity (p=0.03, log rank test). We also performed RT-PCR for mRNA quantification, although no significant differences were observed between normal (n=5) and malignant (n=5). In conclusion, our results corroborate higher frecuency of HO-1 protein expression in gliomas than in normal brain and show a correlation of HO-1 expression with patient survival.Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Salomón, Débora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Zenklusen, Jean C.. Public Health Service. National Institute Of Health; Estados UnidosFil: Robles, Ana. Public Health Service. National Institute Of Health; Estados UnidosFil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Facchinetti, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaHeme Oxygenase in Biology & Medicine: 6Th International Congress on Heme oxygenasesMiami BeachEstados UnidosComité internacional del 6Th International Congress on Heme oxygenasesComité internacional del 6Th International Congress on Heme oxygenases2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242796Expression of heme oxygenase-1 in human gliomas; Heme Oxygenase in Biology & Medicine: 6Th International Congress on Heme oxygenases; Miami Beach; Estados Unidos; 2009; 33-33CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:18Zoai:ri.conicet.gov.ar:11336/242796instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:19.221CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of heme oxygenase-1 in human gliomas
title Expression of heme oxygenase-1 in human gliomas
spellingShingle Expression of heme oxygenase-1 in human gliomas
Gandini, Norberto Ariel
HEMEOXYGENASE-1
GLIOMAS
CANCER
EXPRESSION
title_short Expression of heme oxygenase-1 in human gliomas
title_full Expression of heme oxygenase-1 in human gliomas
title_fullStr Expression of heme oxygenase-1 in human gliomas
title_full_unstemmed Expression of heme oxygenase-1 in human gliomas
title_sort Expression of heme oxygenase-1 in human gliomas
dc.creator.none.fl_str_mv Gandini, Norberto Ariel
Salomón, Débora
Fermento, María Eugenia
Zenklusen, Jean C.
Robles, Ana
Curino, Alejandro Carlos
Facchinetti, María Marta
author Gandini, Norberto Ariel
author_facet Gandini, Norberto Ariel
Salomón, Débora
Fermento, María Eugenia
Zenklusen, Jean C.
Robles, Ana
Curino, Alejandro Carlos
Facchinetti, María Marta
author_role author
author2 Salomón, Débora
Fermento, María Eugenia
Zenklusen, Jean C.
Robles, Ana
Curino, Alejandro Carlos
Facchinetti, María Marta
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv HEMEOXYGENASE-1
GLIOMAS
CANCER
EXPRESSION
topic HEMEOXYGENASE-1
GLIOMAS
CANCER
EXPRESSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The vast majority of experiments performed in different tumor cells indicate that HO-1 is a potent cytoprotective and antiapoptotic enzyme wich improves survival of cancer cells subjected to different kinds of therapy. In glioma tumours HO-1 has been shown to be upregulated as compared with normal brain, although the significance of this upregulation is not clear. Therefore, the aim of our study was to perform a wide screening of heme oxygenase-1 (HO1) expression in glioma by using tissue microarrays (TMA) containing  astrocytomas (18), oligodendrogliomas (29), mixed tumors (12), glioblastoma multiforme (GBM. 57) and normal brain (18) and to correlate protein expression with patient clinic pathological data. HO-1 showed cytoplasmic localization, although a few cells also presented nuclear staining. Nuclear HO-1 was confirmed in T98 and U87 cells. HO-1 was positive in 51% of GBM, 62% astrocytomas, 61%oligodendrogliomas, 42% of mixed and 22% of normal tissue. We found differences in HO-1 positivity rates between normal brain and oligodendrogliomas (p=0.012) and astrocytomas (p=0.027). Comparison by the Kaplan Meier method revealed a sgnificant decreasein overall survival of astrocytoma patients with HO-1 positivity (p=0.03, log rank test). We also performed RT-PCR for mRNA quantification, although no significant differences were observed between normal (n=5) and malignant (n=5). In conclusion, our results corroborate higher frecuency of HO-1 protein expression in gliomas than in normal brain and show a correlation of HO-1 expression with patient survival.
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salomón, Débora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Zenklusen, Jean C.. Public Health Service. National Institute Of Health; Estados Unidos
Fil: Robles, Ana. Public Health Service. National Institute Of Health; Estados Unidos
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Facchinetti, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Heme Oxygenase in Biology & Medicine: 6Th International Congress on Heme oxygenases
Miami Beach
Estados Unidos
Comité internacional del 6Th International Congress on Heme oxygenases
description The vast majority of experiments performed in different tumor cells indicate that HO-1 is a potent cytoprotective and antiapoptotic enzyme wich improves survival of cancer cells subjected to different kinds of therapy. In glioma tumours HO-1 has been shown to be upregulated as compared with normal brain, although the significance of this upregulation is not clear. Therefore, the aim of our study was to perform a wide screening of heme oxygenase-1 (HO1) expression in glioma by using tissue microarrays (TMA) containing  astrocytomas (18), oligodendrogliomas (29), mixed tumors (12), glioblastoma multiforme (GBM. 57) and normal brain (18) and to correlate protein expression with patient clinic pathological data. HO-1 showed cytoplasmic localization, although a few cells also presented nuclear staining. Nuclear HO-1 was confirmed in T98 and U87 cells. HO-1 was positive in 51% of GBM, 62% astrocytomas, 61%oligodendrogliomas, 42% of mixed and 22% of normal tissue. We found differences in HO-1 positivity rates between normal brain and oligodendrogliomas (p=0.012) and astrocytomas (p=0.027). Comparison by the Kaplan Meier method revealed a sgnificant decreasein overall survival of astrocytoma patients with HO-1 positivity (p=0.03, log rank test). We also performed RT-PCR for mRNA quantification, although no significant differences were observed between normal (n=5) and malignant (n=5). In conclusion, our results corroborate higher frecuency of HO-1 protein expression in gliomas than in normal brain and show a correlation of HO-1 expression with patient survival.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242796
Expression of heme oxygenase-1 in human gliomas; Heme Oxygenase in Biology & Medicine: 6Th International Congress on Heme oxygenases; Miami Beach; Estados Unidos; 2009; 33-33
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242796
identifier_str_mv Expression of heme oxygenase-1 in human gliomas; Heme Oxygenase in Biology & Medicine: 6Th International Congress on Heme oxygenases; Miami Beach; Estados Unidos; 2009; 33-33
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Comité internacional del 6Th International Congress on Heme oxygenases
publisher.none.fl_str_mv Comité internacional del 6Th International Congress on Heme oxygenases
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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