Expression of HO-1 in human lung cancer and its correlation with clinical data
- Autores
- Mendizabal, Javier E.; Degese, Sol; Gandini, Norberto Ariel; Coso, Omar Adrian; Curino, Alejandro Carlos; Facchinetti, Maria Marta
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- An increasing amount of evidence indicates that HO-1 activation may play a role in carcinogenesis, potently influencing the growth and metastasis of tumors. Although several reports show a role for HO-1 in lung cancer, little is known about enzyme expression in human lung cancer specimens. Therefore, the aim of this work was to sudy HO-1 expression in human lung carcinoma samples and its correlation with clinicopathological data. As a first approach we tested HO-1 expression in 16 paraffin embedded tumor specimens. Positive protein immunostaining was observed in 75% (12) of the samples analyzed and HO-1 was mainly expressed by carcinoma cells. In order to perform a wide screening of HO-1 expression in lung cancer we used a tissue microarray (TMA) consisting of 330 cores including non-malignant lung tissue, adenocarcinoma, squamous cell carcinoma, carcinoid tumors, small cell carcinoma and large cell carcinoma.92% of tumor samples were positive for HO-1 and protein expression correlated with advanced satges (p=0.021, X2), lymph node involvement (p=0.025), and gender (p=0.036). Additionally we performed RT-PCR in 19 samples of human lung carcinomas, detecting HO-1 mRNA in 84% of them. A significant correlation was observed between HO-1 mRNA levels and smoking habit (p=0.004) and T staging (TNM, p=0.001). In summary, these results present additional evidence of an important role for HO-1 in lung cancer.
Fil: Mendizabal, Javier E.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina
Fil: Degese, Sol. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Coso, Omar Adrian. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Heme Oxygenase in Biology & Medicine: 6Th International Congress on Heme oxygenases
Miami Beach
Estados Unidos
Comité internacional del 6Th International Congress on Heme oxygenases - Materia
-
LUNG
CANCER
HEMEOXYGENASE-1
TISSUE MICROARRAY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/242794
Ver los metadatos del registro completo
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Expression of HO-1 in human lung cancer and its correlation with clinical dataMendizabal, Javier E.Degese, SolGandini, Norberto ArielCoso, Omar AdrianCurino, Alejandro CarlosFacchinetti, Maria MartaLUNGCANCERHEMEOXYGENASE-1TISSUE MICROARRAYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3An increasing amount of evidence indicates that HO-1 activation may play a role in carcinogenesis, potently influencing the growth and metastasis of tumors. Although several reports show a role for HO-1 in lung cancer, little is known about enzyme expression in human lung cancer specimens. Therefore, the aim of this work was to sudy HO-1 expression in human lung carcinoma samples and its correlation with clinicopathological data. As a first approach we tested HO-1 expression in 16 paraffin embedded tumor specimens. Positive protein immunostaining was observed in 75% (12) of the samples analyzed and HO-1 was mainly expressed by carcinoma cells. In order to perform a wide screening of HO-1 expression in lung cancer we used a tissue microarray (TMA) consisting of 330 cores including non-malignant lung tissue, adenocarcinoma, squamous cell carcinoma, carcinoid tumors, small cell carcinoma and large cell carcinoma.92% of tumor samples were positive for HO-1 and protein expression correlated with advanced satges (p=0.021, X2), lymph node involvement (p=0.025), and gender (p=0.036). Additionally we performed RT-PCR in 19 samples of human lung carcinomas, detecting HO-1 mRNA in 84% of them. A significant correlation was observed between HO-1 mRNA levels and smoking habit (p=0.004) and T staging (TNM, p=0.001). In summary, these results present additional evidence of an important role for HO-1 in lung cancer.Fil: Mendizabal, Javier E.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; ArgentinaFil: Degese, Sol. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; ArgentinaFil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Coso, Omar Adrian. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; ArgentinaFil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaHeme Oxygenase in Biology & Medicine: 6Th International Congress on Heme oxygenasesMiami BeachEstados UnidosComité internacional del 6Th International Congress on Heme oxygenasesComité internacional del 6Th International Congress on Heme oxygenases2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242794Expression of HO-1 in human lung cancer and its correlation with clinical data; Heme Oxygenase in Biology & Medicine: 6Th International Congress on Heme oxygenases; Miami Beach; Estados Unidos; 2009; 42-42CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:11Zoai:ri.conicet.gov.ar:11336/242794instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:11.802CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Expression of HO-1 in human lung cancer and its correlation with clinical data |
| title |
Expression of HO-1 in human lung cancer and its correlation with clinical data |
| spellingShingle |
Expression of HO-1 in human lung cancer and its correlation with clinical data Mendizabal, Javier E. LUNG CANCER HEMEOXYGENASE-1 TISSUE MICROARRAY |
| title_short |
Expression of HO-1 in human lung cancer and its correlation with clinical data |
| title_full |
Expression of HO-1 in human lung cancer and its correlation with clinical data |
| title_fullStr |
Expression of HO-1 in human lung cancer and its correlation with clinical data |
| title_full_unstemmed |
Expression of HO-1 in human lung cancer and its correlation with clinical data |
| title_sort |
Expression of HO-1 in human lung cancer and its correlation with clinical data |
| dc.creator.none.fl_str_mv |
Mendizabal, Javier E. Degese, Sol Gandini, Norberto Ariel Coso, Omar Adrian Curino, Alejandro Carlos Facchinetti, Maria Marta |
| author |
Mendizabal, Javier E. |
| author_facet |
Mendizabal, Javier E. Degese, Sol Gandini, Norberto Ariel Coso, Omar Adrian Curino, Alejandro Carlos Facchinetti, Maria Marta |
| author_role |
author |
| author2 |
Degese, Sol Gandini, Norberto Ariel Coso, Omar Adrian Curino, Alejandro Carlos Facchinetti, Maria Marta |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
LUNG CANCER HEMEOXYGENASE-1 TISSUE MICROARRAY |
| topic |
LUNG CANCER HEMEOXYGENASE-1 TISSUE MICROARRAY |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
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An increasing amount of evidence indicates that HO-1 activation may play a role in carcinogenesis, potently influencing the growth and metastasis of tumors. Although several reports show a role for HO-1 in lung cancer, little is known about enzyme expression in human lung cancer specimens. Therefore, the aim of this work was to sudy HO-1 expression in human lung carcinoma samples and its correlation with clinicopathological data. As a first approach we tested HO-1 expression in 16 paraffin embedded tumor specimens. Positive protein immunostaining was observed in 75% (12) of the samples analyzed and HO-1 was mainly expressed by carcinoma cells. In order to perform a wide screening of HO-1 expression in lung cancer we used a tissue microarray (TMA) consisting of 330 cores including non-malignant lung tissue, adenocarcinoma, squamous cell carcinoma, carcinoid tumors, small cell carcinoma and large cell carcinoma.92% of tumor samples were positive for HO-1 and protein expression correlated with advanced satges (p=0.021, X2), lymph node involvement (p=0.025), and gender (p=0.036). Additionally we performed RT-PCR in 19 samples of human lung carcinomas, detecting HO-1 mRNA in 84% of them. A significant correlation was observed between HO-1 mRNA levels and smoking habit (p=0.004) and T staging (TNM, p=0.001). In summary, these results present additional evidence of an important role for HO-1 in lung cancer. Fil: Mendizabal, Javier E.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina Fil: Degese, Sol. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Coso, Omar Adrian. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Heme Oxygenase in Biology & Medicine: 6Th International Congress on Heme oxygenases Miami Beach Estados Unidos Comité internacional del 6Th International Congress on Heme oxygenases |
| description |
An increasing amount of evidence indicates that HO-1 activation may play a role in carcinogenesis, potently influencing the growth and metastasis of tumors. Although several reports show a role for HO-1 in lung cancer, little is known about enzyme expression in human lung cancer specimens. Therefore, the aim of this work was to sudy HO-1 expression in human lung carcinoma samples and its correlation with clinicopathological data. As a first approach we tested HO-1 expression in 16 paraffin embedded tumor specimens. Positive protein immunostaining was observed in 75% (12) of the samples analyzed and HO-1 was mainly expressed by carcinoma cells. In order to perform a wide screening of HO-1 expression in lung cancer we used a tissue microarray (TMA) consisting of 330 cores including non-malignant lung tissue, adenocarcinoma, squamous cell carcinoma, carcinoid tumors, small cell carcinoma and large cell carcinoma.92% of tumor samples were positive for HO-1 and protein expression correlated with advanced satges (p=0.021, X2), lymph node involvement (p=0.025), and gender (p=0.036). Additionally we performed RT-PCR in 19 samples of human lung carcinomas, detecting HO-1 mRNA in 84% of them. A significant correlation was observed between HO-1 mRNA levels and smoking habit (p=0.004) and T staging (TNM, p=0.001). In summary, these results present additional evidence of an important role for HO-1 in lung cancer. |
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2009 |
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2009 |
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