Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma

Autores
Gandini, Norberto Ariel; Fermento, María Eugenia; Salomón, Débora Gisele; Obiol, Diego Javier; Andrés, Nancy Carolina; Zenklusen, Jean C.; Arevalo, Julian; Blasco, Jorge; Lopez, Alejandro; Facchinetti, Maria Marta; Curino, Alejandro Carlos
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In human glioma tumors, heme oxygenase-1 (HO-1) has been shown to be upregulated both when compared with normal brain tissues and also during oligodendroglioma progression. The cell types that express HO-1 have been shown to be mainly macrophages/microglia and T cells. However, many other reports also demonstrated that cell lines derived from glioma tumors and astrocytes express HO-1 after the occurrence of a wide variety of cell injuries and stressors. In addition, the significance of HO-1 upregulation in glioma had not, so far, been addressed. We therefore aimed at investigating the expression and significance of HO-1 in human glial tumors. For this purpose, we performed a wide screening of HO-1 expression in gliomas by using tissue microarrays containing astrocytomas, oligodendrogliomas, mixed tumors, and normal brain tissues. We subsequently correlated protein expression with patient clinicopathological data. We found differences in HO-1 positivity rates between non-malignant brain (22 %) and gliomas (54 %, p = 0.01). HO-1 was expressed by tumor cells and showed cytoplasmic localization, although 19 % of tumor samples also depicted nuclear staining. Importantly, a significant decrease in the overall survival time of grade II and III astrocytoma patients with HO-1 expression was observed. This result was validated at the mRNA level in a cohort of 105 samples. However, no association of HO-1 nuclear localization with patient survival was detected. In vitro experiments aimed at investigating the role of HO-1 in glioma progression showed that HO-1 modulates glioma cell proliferation, but has no effects on cellular migration. In conclusion, our results corroborate the higher frequency of HO-1 protein expression in gliomas than in normal brain, demonstrate that HO-1 is expressed by glial malignant cells, and show an association of HO-1 expression with patients’ shorter survival time.
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Salomón, Débora Gisele. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Obiol, Diego Javier. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Andrés, Nancy Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Zenklusen, Jean C.. National Institute of Health.The Cancer Genome Atlas Program Office; Estados Unidos
Fil: Arevalo, Julian. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Blasco, Jorge. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Lopez, Alejandro. Laboratorios IACA, Bahia Blanca; Argentina
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Materia
Heme Oxygenase-1
Glioma
Astrocytoma
Survival
Tissue Microarray
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6617

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network_name_str CONICET Digital (CONICET)
spelling Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytomaGandini, Norberto ArielFermento, María EugeniaSalomón, Débora GiseleObiol, Diego JavierAndrés, Nancy CarolinaZenklusen, Jean C.Arevalo, JulianBlasco, JorgeLopez, AlejandroFacchinetti, Maria MartaCurino, Alejandro CarlosHeme Oxygenase-1GliomaAstrocytomaSurvivalTissue Microarrayhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In human glioma tumors, heme oxygenase-1 (HO-1) has been shown to be upregulated both when compared with normal brain tissues and also during oligodendroglioma progression. The cell types that express HO-1 have been shown to be mainly macrophages/microglia and T cells. However, many other reports also demonstrated that cell lines derived from glioma tumors and astrocytes express HO-1 after the occurrence of a wide variety of cell injuries and stressors. In addition, the significance of HO-1 upregulation in glioma had not, so far, been addressed. We therefore aimed at investigating the expression and significance of HO-1 in human glial tumors. For this purpose, we performed a wide screening of HO-1 expression in gliomas by using tissue microarrays containing astrocytomas, oligodendrogliomas, mixed tumors, and normal brain tissues. We subsequently correlated protein expression with patient clinicopathological data. We found differences in HO-1 positivity rates between non-malignant brain (22 %) and gliomas (54 %, p = 0.01). HO-1 was expressed by tumor cells and showed cytoplasmic localization, although 19 % of tumor samples also depicted nuclear staining. Importantly, a significant decrease in the overall survival time of grade II and III astrocytoma patients with HO-1 expression was observed. This result was validated at the mRNA level in a cohort of 105 samples. However, no association of HO-1 nuclear localization with patient survival was detected. In vitro experiments aimed at investigating the role of HO-1 in glioma progression showed that HO-1 modulates glioma cell proliferation, but has no effects on cellular migration. In conclusion, our results corroborate the higher frequency of HO-1 protein expression in gliomas than in normal brain, demonstrate that HO-1 is expressed by glial malignant cells, and show an association of HO-1 expression with patients’ shorter survival time.Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Salomón, Débora Gisele. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Obiol, Diego Javier. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Andrés, Nancy Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Zenklusen, Jean C.. National Institute of Health.The Cancer Genome Atlas Program Office; Estados UnidosFil: Arevalo, Julian. Hospital Municipal General de Agudos Doctor José Penna; ArgentinaFil: Blasco, Jorge. Hospital Municipal General de Agudos Doctor José Penna; ArgentinaFil: Lopez, Alejandro. Laboratorios IACA, Bahia Blanca; ArgentinaFil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaSpringer2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6617Gandini, Norberto Ariel; Fermento, María Eugenia; Salomón, Débora Gisele; Obiol, Diego Javier; Andrés, Nancy Carolina; et al.; Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma; Springer; Tumor Biology; 35; 3; 11-2013; 2803-28151010-4283enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1007/s13277-013-1373-zinfo:eu-repo/semantics/altIdentifier/pmid/24234335info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs13277-013-1373-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:39Zoai:ri.conicet.gov.ar:11336/6617instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:40.242CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma
title Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma
spellingShingle Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma
Gandini, Norberto Ariel
Heme Oxygenase-1
Glioma
Astrocytoma
Survival
Tissue Microarray
title_short Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma
title_full Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma
title_fullStr Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma
title_full_unstemmed Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma
title_sort Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma
dc.creator.none.fl_str_mv Gandini, Norberto Ariel
Fermento, María Eugenia
Salomón, Débora Gisele
Obiol, Diego Javier
Andrés, Nancy Carolina
Zenklusen, Jean C.
Arevalo, Julian
Blasco, Jorge
Lopez, Alejandro
Facchinetti, Maria Marta
Curino, Alejandro Carlos
author Gandini, Norberto Ariel
author_facet Gandini, Norberto Ariel
Fermento, María Eugenia
Salomón, Débora Gisele
Obiol, Diego Javier
Andrés, Nancy Carolina
Zenklusen, Jean C.
Arevalo, Julian
Blasco, Jorge
Lopez, Alejandro
Facchinetti, Maria Marta
Curino, Alejandro Carlos
author_role author
author2 Fermento, María Eugenia
Salomón, Débora Gisele
Obiol, Diego Javier
Andrés, Nancy Carolina
Zenklusen, Jean C.
Arevalo, Julian
Blasco, Jorge
Lopez, Alejandro
Facchinetti, Maria Marta
Curino, Alejandro Carlos
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Heme Oxygenase-1
Glioma
Astrocytoma
Survival
Tissue Microarray
topic Heme Oxygenase-1
Glioma
Astrocytoma
Survival
Tissue Microarray
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In human glioma tumors, heme oxygenase-1 (HO-1) has been shown to be upregulated both when compared with normal brain tissues and also during oligodendroglioma progression. The cell types that express HO-1 have been shown to be mainly macrophages/microglia and T cells. However, many other reports also demonstrated that cell lines derived from glioma tumors and astrocytes express HO-1 after the occurrence of a wide variety of cell injuries and stressors. In addition, the significance of HO-1 upregulation in glioma had not, so far, been addressed. We therefore aimed at investigating the expression and significance of HO-1 in human glial tumors. For this purpose, we performed a wide screening of HO-1 expression in gliomas by using tissue microarrays containing astrocytomas, oligodendrogliomas, mixed tumors, and normal brain tissues. We subsequently correlated protein expression with patient clinicopathological data. We found differences in HO-1 positivity rates between non-malignant brain (22 %) and gliomas (54 %, p = 0.01). HO-1 was expressed by tumor cells and showed cytoplasmic localization, although 19 % of tumor samples also depicted nuclear staining. Importantly, a significant decrease in the overall survival time of grade II and III astrocytoma patients with HO-1 expression was observed. This result was validated at the mRNA level in a cohort of 105 samples. However, no association of HO-1 nuclear localization with patient survival was detected. In vitro experiments aimed at investigating the role of HO-1 in glioma progression showed that HO-1 modulates glioma cell proliferation, but has no effects on cellular migration. In conclusion, our results corroborate the higher frequency of HO-1 protein expression in gliomas than in normal brain, demonstrate that HO-1 is expressed by glial malignant cells, and show an association of HO-1 expression with patients’ shorter survival time.
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Salomón, Débora Gisele. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Obiol, Diego Javier. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Andrés, Nancy Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Zenklusen, Jean C.. National Institute of Health.The Cancer Genome Atlas Program Office; Estados Unidos
Fil: Arevalo, Julian. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Blasco, Jorge. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Lopez, Alejandro. Laboratorios IACA, Bahia Blanca; Argentina
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
description In human glioma tumors, heme oxygenase-1 (HO-1) has been shown to be upregulated both when compared with normal brain tissues and also during oligodendroglioma progression. The cell types that express HO-1 have been shown to be mainly macrophages/microglia and T cells. However, many other reports also demonstrated that cell lines derived from glioma tumors and astrocytes express HO-1 after the occurrence of a wide variety of cell injuries and stressors. In addition, the significance of HO-1 upregulation in glioma had not, so far, been addressed. We therefore aimed at investigating the expression and significance of HO-1 in human glial tumors. For this purpose, we performed a wide screening of HO-1 expression in gliomas by using tissue microarrays containing astrocytomas, oligodendrogliomas, mixed tumors, and normal brain tissues. We subsequently correlated protein expression with patient clinicopathological data. We found differences in HO-1 positivity rates between non-malignant brain (22 %) and gliomas (54 %, p = 0.01). HO-1 was expressed by tumor cells and showed cytoplasmic localization, although 19 % of tumor samples also depicted nuclear staining. Importantly, a significant decrease in the overall survival time of grade II and III astrocytoma patients with HO-1 expression was observed. This result was validated at the mRNA level in a cohort of 105 samples. However, no association of HO-1 nuclear localization with patient survival was detected. In vitro experiments aimed at investigating the role of HO-1 in glioma progression showed that HO-1 modulates glioma cell proliferation, but has no effects on cellular migration. In conclusion, our results corroborate the higher frequency of HO-1 protein expression in gliomas than in normal brain, demonstrate that HO-1 is expressed by glial malignant cells, and show an association of HO-1 expression with patients’ shorter survival time.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6617
Gandini, Norberto Ariel; Fermento, María Eugenia; Salomón, Débora Gisele; Obiol, Diego Javier; Andrés, Nancy Carolina; et al.; Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma; Springer; Tumor Biology; 35; 3; 11-2013; 2803-2815
1010-4283
url http://hdl.handle.net/11336/6617
identifier_str_mv Gandini, Norberto Ariel; Fermento, María Eugenia; Salomón, Débora Gisele; Obiol, Diego Javier; Andrés, Nancy Carolina; et al.; Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma; Springer; Tumor Biology; 35; 3; 11-2013; 2803-2815
1010-4283
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1007/s13277-013-1373-z
info:eu-repo/semantics/altIdentifier/pmid/24234335
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs13277-013-1373-z
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