Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma
- Autores
- Gandini, Norberto Ariel; Fermento, María Eugenia; Salomón, Débora Gisele; Obiol, Diego Javier; Andrés, Nancy Carolina; Zenklusen, Jean C.; Arevalo, Julian; Blasco, Jorge; Lopez, Alejandro; Facchinetti, Maria Marta; Curino, Alejandro Carlos
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In human glioma tumors, heme oxygenase-1 (HO-1) has been shown to be upregulated both when compared with normal brain tissues and also during oligodendroglioma progression. The cell types that express HO-1 have been shown to be mainly macrophages/microglia and T cells. However, many other reports also demonstrated that cell lines derived from glioma tumors and astrocytes express HO-1 after the occurrence of a wide variety of cell injuries and stressors. In addition, the significance of HO-1 upregulation in glioma had not, so far, been addressed. We therefore aimed at investigating the expression and significance of HO-1 in human glial tumors. For this purpose, we performed a wide screening of HO-1 expression in gliomas by using tissue microarrays containing astrocytomas, oligodendrogliomas, mixed tumors, and normal brain tissues. We subsequently correlated protein expression with patient clinicopathological data. We found differences in HO-1 positivity rates between non-malignant brain (22 %) and gliomas (54 %, p = 0.01). HO-1 was expressed by tumor cells and showed cytoplasmic localization, although 19 % of tumor samples also depicted nuclear staining. Importantly, a significant decrease in the overall survival time of grade II and III astrocytoma patients with HO-1 expression was observed. This result was validated at the mRNA level in a cohort of 105 samples. However, no association of HO-1 nuclear localization with patient survival was detected. In vitro experiments aimed at investigating the role of HO-1 in glioma progression showed that HO-1 modulates glioma cell proliferation, but has no effects on cellular migration. In conclusion, our results corroborate the higher frequency of HO-1 protein expression in gliomas than in normal brain, demonstrate that HO-1 is expressed by glial malignant cells, and show an association of HO-1 expression with patients’ shorter survival time.
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Salomón, Débora Gisele. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Obiol, Diego Javier. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Andrés, Nancy Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Zenklusen, Jean C.. National Institute of Health.The Cancer Genome Atlas Program Office; Estados Unidos
Fil: Arevalo, Julian. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Blasco, Jorge. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Lopez, Alejandro. Laboratorios IACA, Bahia Blanca; Argentina
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina - Materia
-
Heme Oxygenase-1
Glioma
Astrocytoma
Survival
Tissue Microarray - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/6617
Ver los metadatos del registro completo
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Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytomaGandini, Norberto ArielFermento, María EugeniaSalomón, Débora GiseleObiol, Diego JavierAndrés, Nancy CarolinaZenklusen, Jean C.Arevalo, JulianBlasco, JorgeLopez, AlejandroFacchinetti, Maria MartaCurino, Alejandro CarlosHeme Oxygenase-1GliomaAstrocytomaSurvivalTissue Microarrayhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In human glioma tumors, heme oxygenase-1 (HO-1) has been shown to be upregulated both when compared with normal brain tissues and also during oligodendroglioma progression. The cell types that express HO-1 have been shown to be mainly macrophages/microglia and T cells. However, many other reports also demonstrated that cell lines derived from glioma tumors and astrocytes express HO-1 after the occurrence of a wide variety of cell injuries and stressors. In addition, the significance of HO-1 upregulation in glioma had not, so far, been addressed. We therefore aimed at investigating the expression and significance of HO-1 in human glial tumors. For this purpose, we performed a wide screening of HO-1 expression in gliomas by using tissue microarrays containing astrocytomas, oligodendrogliomas, mixed tumors, and normal brain tissues. We subsequently correlated protein expression with patient clinicopathological data. We found differences in HO-1 positivity rates between non-malignant brain (22 %) and gliomas (54 %, p = 0.01). HO-1 was expressed by tumor cells and showed cytoplasmic localization, although 19 % of tumor samples also depicted nuclear staining. Importantly, a significant decrease in the overall survival time of grade II and III astrocytoma patients with HO-1 expression was observed. This result was validated at the mRNA level in a cohort of 105 samples. However, no association of HO-1 nuclear localization with patient survival was detected. In vitro experiments aimed at investigating the role of HO-1 in glioma progression showed that HO-1 modulates glioma cell proliferation, but has no effects on cellular migration. In conclusion, our results corroborate the higher frequency of HO-1 protein expression in gliomas than in normal brain, demonstrate that HO-1 is expressed by glial malignant cells, and show an association of HO-1 expression with patients’ shorter survival time.Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Salomón, Débora Gisele. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Obiol, Diego Javier. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Andrés, Nancy Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Zenklusen, Jean C.. National Institute of Health.The Cancer Genome Atlas Program Office; Estados UnidosFil: Arevalo, Julian. Hospital Municipal General de Agudos Doctor José Penna; ArgentinaFil: Blasco, Jorge. Hospital Municipal General de Agudos Doctor José Penna; ArgentinaFil: Lopez, Alejandro. Laboratorios IACA, Bahia Blanca; ArgentinaFil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaSpringer2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6617Gandini, Norberto Ariel; Fermento, María Eugenia; Salomón, Débora Gisele; Obiol, Diego Javier; Andrés, Nancy Carolina; et al.; Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma; Springer; Tumor Biology; 35; 3; 11-2013; 2803-28151010-4283enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1007/s13277-013-1373-zinfo:eu-repo/semantics/altIdentifier/pmid/24234335info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs13277-013-1373-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:10Zoai:ri.conicet.gov.ar:11336/6617instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:10.557CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma |
| title |
Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma |
| spellingShingle |
Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma Gandini, Norberto Ariel Heme Oxygenase-1 Glioma Astrocytoma Survival Tissue Microarray |
| title_short |
Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma |
| title_full |
Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma |
| title_fullStr |
Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma |
| title_full_unstemmed |
Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma |
| title_sort |
Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma |
| dc.creator.none.fl_str_mv |
Gandini, Norberto Ariel Fermento, María Eugenia Salomón, Débora Gisele Obiol, Diego Javier Andrés, Nancy Carolina Zenklusen, Jean C. Arevalo, Julian Blasco, Jorge Lopez, Alejandro Facchinetti, Maria Marta Curino, Alejandro Carlos |
| author |
Gandini, Norberto Ariel |
| author_facet |
Gandini, Norberto Ariel Fermento, María Eugenia Salomón, Débora Gisele Obiol, Diego Javier Andrés, Nancy Carolina Zenklusen, Jean C. Arevalo, Julian Blasco, Jorge Lopez, Alejandro Facchinetti, Maria Marta Curino, Alejandro Carlos |
| author_role |
author |
| author2 |
Fermento, María Eugenia Salomón, Débora Gisele Obiol, Diego Javier Andrés, Nancy Carolina Zenklusen, Jean C. Arevalo, Julian Blasco, Jorge Lopez, Alejandro Facchinetti, Maria Marta Curino, Alejandro Carlos |
| author2_role |
author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Heme Oxygenase-1 Glioma Astrocytoma Survival Tissue Microarray |
| topic |
Heme Oxygenase-1 Glioma Astrocytoma Survival Tissue Microarray |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
In human glioma tumors, heme oxygenase-1 (HO-1) has been shown to be upregulated both when compared with normal brain tissues and also during oligodendroglioma progression. The cell types that express HO-1 have been shown to be mainly macrophages/microglia and T cells. However, many other reports also demonstrated that cell lines derived from glioma tumors and astrocytes express HO-1 after the occurrence of a wide variety of cell injuries and stressors. In addition, the significance of HO-1 upregulation in glioma had not, so far, been addressed. We therefore aimed at investigating the expression and significance of HO-1 in human glial tumors. For this purpose, we performed a wide screening of HO-1 expression in gliomas by using tissue microarrays containing astrocytomas, oligodendrogliomas, mixed tumors, and normal brain tissues. We subsequently correlated protein expression with patient clinicopathological data. We found differences in HO-1 positivity rates between non-malignant brain (22 %) and gliomas (54 %, p = 0.01). HO-1 was expressed by tumor cells and showed cytoplasmic localization, although 19 % of tumor samples also depicted nuclear staining. Importantly, a significant decrease in the overall survival time of grade II and III astrocytoma patients with HO-1 expression was observed. This result was validated at the mRNA level in a cohort of 105 samples. However, no association of HO-1 nuclear localization with patient survival was detected. In vitro experiments aimed at investigating the role of HO-1 in glioma progression showed that HO-1 modulates glioma cell proliferation, but has no effects on cellular migration. In conclusion, our results corroborate the higher frequency of HO-1 protein expression in gliomas than in normal brain, demonstrate that HO-1 is expressed by glial malignant cells, and show an association of HO-1 expression with patients’ shorter survival time. Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina Fil: Salomón, Débora Gisele. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina Fil: Obiol, Diego Javier. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina Fil: Andrés, Nancy Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina Fil: Zenklusen, Jean C.. National Institute of Health.The Cancer Genome Atlas Program Office; Estados Unidos Fil: Arevalo, Julian. Hospital Municipal General de Agudos Doctor José Penna; Argentina Fil: Blasco, Jorge. Hospital Municipal General de Agudos Doctor José Penna; Argentina Fil: Lopez, Alejandro. Laboratorios IACA, Bahia Blanca; Argentina Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Bahia Blanca. Instituto de Invest.bioquimicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina |
| description |
In human glioma tumors, heme oxygenase-1 (HO-1) has been shown to be upregulated both when compared with normal brain tissues and also during oligodendroglioma progression. The cell types that express HO-1 have been shown to be mainly macrophages/microglia and T cells. However, many other reports also demonstrated that cell lines derived from glioma tumors and astrocytes express HO-1 after the occurrence of a wide variety of cell injuries and stressors. In addition, the significance of HO-1 upregulation in glioma had not, so far, been addressed. We therefore aimed at investigating the expression and significance of HO-1 in human glial tumors. For this purpose, we performed a wide screening of HO-1 expression in gliomas by using tissue microarrays containing astrocytomas, oligodendrogliomas, mixed tumors, and normal brain tissues. We subsequently correlated protein expression with patient clinicopathological data. We found differences in HO-1 positivity rates between non-malignant brain (22 %) and gliomas (54 %, p = 0.01). HO-1 was expressed by tumor cells and showed cytoplasmic localization, although 19 % of tumor samples also depicted nuclear staining. Importantly, a significant decrease in the overall survival time of grade II and III astrocytoma patients with HO-1 expression was observed. This result was validated at the mRNA level in a cohort of 105 samples. However, no association of HO-1 nuclear localization with patient survival was detected. In vitro experiments aimed at investigating the role of HO-1 in glioma progression showed that HO-1 modulates glioma cell proliferation, but has no effects on cellular migration. In conclusion, our results corroborate the higher frequency of HO-1 protein expression in gliomas than in normal brain, demonstrate that HO-1 is expressed by glial malignant cells, and show an association of HO-1 expression with patients’ shorter survival time. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013-11 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/6617 Gandini, Norberto Ariel; Fermento, María Eugenia; Salomón, Débora Gisele; Obiol, Diego Javier; Andrés, Nancy Carolina; et al.; Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma; Springer; Tumor Biology; 35; 3; 11-2013; 2803-2815 1010-4283 |
| url |
http://hdl.handle.net/11336/6617 |
| identifier_str_mv |
Gandini, Norberto Ariel; Fermento, María Eugenia; Salomón, Débora Gisele; Obiol, Diego Javier; Andrés, Nancy Carolina; et al.; Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma; Springer; Tumor Biology; 35; 3; 11-2013; 2803-2815 1010-4283 |
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eng |
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eng |
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