Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model

Autores
Hagen, Martine Kienzle; Ludke, Ana; Araujo, Alex Sander; Mendes, Roberta Hack; Fernandes, Tânia Gatelli; Mandarino, Jose Marcos Gontijo; Llesuy, Susana Francisca; Vogt de Jong, Erna; Belló Klein, Adriane
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study analyzed and compared the content of isoflavones in 2 soy products the effectiveness of isoflavones as antioxidants in vitro and demonstrated the antioxidant effect of a soy diet in rats with myocardial infarction (MI). Isoflavone content was analyzed in soybean hypocotyl (SH) and isolated soy protein (ISP). The quality (TAR) and quantity (TRAP) of antioxidants present in the samples was quantified. The amount of daidzin was higher in SH (9 times) and genistein in ISP (5 times). SH presented a 3-fold increase in TAR while both products exhibited same TRAP. The rats were fed an ISP diet for 9 weeks. Animals were distributed among 6 treatment groups: (i) Sham Casein; (ii) Infarct Casein < 25%; (iii) Infarct Casein > 25%; (iv) Sham Soy; (v) Infarct Soy < 25%; and (vi) Infarct Soy > 25%. MI was induced 5 weeks after the commencement of the diets. Lipid peroxidation (LPO) antioxidant enzyme activity and levels of nitrites/nitrates were determined in blood. Rats receiving the ISP diet demonstrated increased activity of antioxidant enzyme activity and nitrite/ nitrate content. In addition the increase in LPO seen in rats subjected to MI was significantly mitigated when the ISP diet was given. These findings suggest a nutritional approach of using a soy-based diet for the prevention of oxidative-stress-related diseases such as heart failure.
Fil: Hagen, Martine Kienzle. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Ludke, Ana. University of Manitoba; Canadá
Fil: Araujo, Alex Sander. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mendes, Roberta Hack. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fernandes, Tânia Gatelli. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mandarino, Jose Marcos Gontijo. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Llesuy, Susana Francisca. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vogt de Jong, Erna. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; Brasil
Materia
Antioxidant Enzymes
Antioxidant Potential
Isolated Soy Protein
Lipid Peroxidation
Myocardial Infarction
Nitric Oxide
Soybean Hypocotyl
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67382
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67382
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease modelHagen, Martine KienzleLudke, AnaAraujo, Alex SanderMendes, Roberta HackFernandes, Tânia GatelliMandarino, Jose Marcos GontijoLlesuy, Susana FranciscaVogt de Jong, ErnaBelló Klein, AdrianeAntioxidant EnzymesAntioxidant PotentialIsolated Soy ProteinLipid PeroxidationMyocardial InfarctionNitric OxideSoybean Hypocotylhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3This study analyzed and compared the content of isoflavones in 2 soy products the effectiveness of isoflavones as antioxidants in vitro and demonstrated the antioxidant effect of a soy diet in rats with myocardial infarction (MI). Isoflavone content was analyzed in soybean hypocotyl (SH) and isolated soy protein (ISP). The quality (TAR) and quantity (TRAP) of antioxidants present in the samples was quantified. The amount of daidzin was higher in SH (9 times) and genistein in ISP (5 times). SH presented a 3-fold increase in TAR while both products exhibited same TRAP. The rats were fed an ISP diet for 9 weeks. Animals were distributed among 6 treatment groups: (i) Sham Casein; (ii) Infarct Casein < 25%; (iii) Infarct Casein > 25%; (iv) Sham Soy; (v) Infarct Soy < 25%; and (vi) Infarct Soy > 25%. MI was induced 5 weeks after the commencement of the diets. Lipid peroxidation (LPO) antioxidant enzyme activity and levels of nitrites/nitrates were determined in blood. Rats receiving the ISP diet demonstrated increased activity of antioxidant enzyme activity and nitrite/ nitrate content. In addition the increase in LPO seen in rats subjected to MI was significantly mitigated when the ISP diet was given. These findings suggest a nutritional approach of using a soy-based diet for the prevention of oxidative-stress-related diseases such as heart failure.Fil: Hagen, Martine Kienzle. Universidade Federal do Rio Grande do Sul; BrasilFil: Ludke, Ana. University of Manitoba; CanadáFil: Araujo, Alex Sander. Universidade Federal do Rio Grande do Sul; BrasilFil: Mendes, Roberta Hack. Universidade Federal do Rio Grande do Sul; BrasilFil: Fernandes, Tânia Gatelli. Universidade Federal do Rio Grande do Sul; BrasilFil: Mandarino, Jose Marcos Gontijo. Universidade Federal do Rio Grande do Sul; BrasilFil: Llesuy, Susana Francisca. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vogt de Jong, Erna. Universidade Federal do Rio Grande do Sul; BrasilFil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; BrasilNational Research Council Canada-NRC Research Press2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67382Hagen, Martine Kienzle; Ludke, Ana; Araujo, Alex Sander; Mendes, Roberta Hack; Fernandes, Tânia Gatelli; et al.; Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model; National Research Council Canada-NRC Research Press; Canadian Journal Of Physiology And Pharmacology; 90; 8; 7-2012; 1095-11030008-4212CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1139/y2012-028info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/y2012-028info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:02Zoai:ri.conicet.gov.ar:11336/67382instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:02.919CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model
title Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model
spellingShingle Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model
Hagen, Martine Kienzle
Antioxidant Enzymes
Antioxidant Potential
Isolated Soy Protein
Lipid Peroxidation
Myocardial Infarction
Nitric Oxide
Soybean Hypocotyl
title_short Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model
title_full Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model
title_fullStr Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model
title_full_unstemmed Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model
title_sort Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model
dc.creator.none.fl_str_mv Hagen, Martine Kienzle
Ludke, Ana
Araujo, Alex Sander
Mendes, Roberta Hack
Fernandes, Tânia Gatelli
Mandarino, Jose Marcos Gontijo
Llesuy, Susana Francisca
Vogt de Jong, Erna
Belló Klein, Adriane
author Hagen, Martine Kienzle
author_facet Hagen, Martine Kienzle
Ludke, Ana
Araujo, Alex Sander
Mendes, Roberta Hack
Fernandes, Tânia Gatelli
Mandarino, Jose Marcos Gontijo
Llesuy, Susana Francisca
Vogt de Jong, Erna
Belló Klein, Adriane
author_role author
author2 Ludke, Ana
Araujo, Alex Sander
Mendes, Roberta Hack
Fernandes, Tânia Gatelli
Mandarino, Jose Marcos Gontijo
Llesuy, Susana Francisca
Vogt de Jong, Erna
Belló Klein, Adriane
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Antioxidant Enzymes
Antioxidant Potential
Isolated Soy Protein
Lipid Peroxidation
Myocardial Infarction
Nitric Oxide
Soybean Hypocotyl
topic Antioxidant Enzymes
Antioxidant Potential
Isolated Soy Protein
Lipid Peroxidation
Myocardial Infarction
Nitric Oxide
Soybean Hypocotyl
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv This study analyzed and compared the content of isoflavones in 2 soy products the effectiveness of isoflavones as antioxidants in vitro and demonstrated the antioxidant effect of a soy diet in rats with myocardial infarction (MI). Isoflavone content was analyzed in soybean hypocotyl (SH) and isolated soy protein (ISP). The quality (TAR) and quantity (TRAP) of antioxidants present in the samples was quantified. The amount of daidzin was higher in SH (9 times) and genistein in ISP (5 times). SH presented a 3-fold increase in TAR while both products exhibited same TRAP. The rats were fed an ISP diet for 9 weeks. Animals were distributed among 6 treatment groups: (i) Sham Casein; (ii) Infarct Casein < 25%; (iii) Infarct Casein > 25%; (iv) Sham Soy; (v) Infarct Soy < 25%; and (vi) Infarct Soy > 25%. MI was induced 5 weeks after the commencement of the diets. Lipid peroxidation (LPO) antioxidant enzyme activity and levels of nitrites/nitrates were determined in blood. Rats receiving the ISP diet demonstrated increased activity of antioxidant enzyme activity and nitrite/ nitrate content. In addition the increase in LPO seen in rats subjected to MI was significantly mitigated when the ISP diet was given. These findings suggest a nutritional approach of using a soy-based diet for the prevention of oxidative-stress-related diseases such as heart failure.
Fil: Hagen, Martine Kienzle. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Ludke, Ana. University of Manitoba; Canadá
Fil: Araujo, Alex Sander. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mendes, Roberta Hack. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fernandes, Tânia Gatelli. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mandarino, Jose Marcos Gontijo. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Llesuy, Susana Francisca. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vogt de Jong, Erna. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; Brasil
description This study analyzed and compared the content of isoflavones in 2 soy products the effectiveness of isoflavones as antioxidants in vitro and demonstrated the antioxidant effect of a soy diet in rats with myocardial infarction (MI). Isoflavone content was analyzed in soybean hypocotyl (SH) and isolated soy protein (ISP). The quality (TAR) and quantity (TRAP) of antioxidants present in the samples was quantified. The amount of daidzin was higher in SH (9 times) and genistein in ISP (5 times). SH presented a 3-fold increase in TAR while both products exhibited same TRAP. The rats were fed an ISP diet for 9 weeks. Animals were distributed among 6 treatment groups: (i) Sham Casein; (ii) Infarct Casein < 25%; (iii) Infarct Casein > 25%; (iv) Sham Soy; (v) Infarct Soy < 25%; and (vi) Infarct Soy > 25%. MI was induced 5 weeks after the commencement of the diets. Lipid peroxidation (LPO) antioxidant enzyme activity and levels of nitrites/nitrates were determined in blood. Rats receiving the ISP diet demonstrated increased activity of antioxidant enzyme activity and nitrite/ nitrate content. In addition the increase in LPO seen in rats subjected to MI was significantly mitigated when the ISP diet was given. These findings suggest a nutritional approach of using a soy-based diet for the prevention of oxidative-stress-related diseases such as heart failure.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67382
Hagen, Martine Kienzle; Ludke, Ana; Araujo, Alex Sander; Mendes, Roberta Hack; Fernandes, Tânia Gatelli; et al.; Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model; National Research Council Canada-NRC Research Press; Canadian Journal Of Physiology And Pharmacology; 90; 8; 7-2012; 1095-1103
0008-4212
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67382
identifier_str_mv Hagen, Martine Kienzle; Ludke, Ana; Araujo, Alex Sander; Mendes, Roberta Hack; Fernandes, Tânia Gatelli; et al.; Antioxidant characterization of soy derived products in vitro and the effect of a soy diet on peripheral markers of oxidative stress in a heart disease model; National Research Council Canada-NRC Research Press; Canadian Journal Of Physiology And Pharmacology; 90; 8; 7-2012; 1095-1103
0008-4212
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1139/y2012-028
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/y2012-028
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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