Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats

Autores
González, Germán Esteban; Rabald, Steffen; Briest, Wilfried; Gelpi, Ricardo Jorge; Seropian, Ignacio Miguel; Zimmer, Heinz Gerd; Deten, Alexander
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: In this study the effect of ribose on heart function and infarct-size was analyzed 6 h after myocardial infarction (MI) in rats. Methods: Continuous i.v.-infusion of NaCl or ribose (200 mg/ kg/h) was started one day prior to induction of MI in female Sprague-Dawley rats which was done by ligation of the left coronary artery. Six hours after MI heart function was measured with 3F tip catheter, cardiac output by thermodilution method. Thereafter the ischemic area was delineated by Evans Blue infusion, and the infarct area was visualized by triphenyltetrazolium chloride staining. The mRNA expression of interleukin (IL)-1β, IL-6, matrixmetalloproteinase (MMP)-8, and -9 was measured by ribonuclease protection assay. Results: Heart function was severely depressed 6 hours after coronary artery occlusion, but recovered significantly under the influence of ribose. Left ventricular (LV) systolic pressure (LVSP) and contractility (LVdP/dtmax) were restored to the normal levels of sham-operated animals, while parameters of LV relaxation (LVdP/dtmin and time constant of relaxation τ) were impaired compared to sham-operated animals, but significantly improved by ribose treatment compared to shamtreated MI-rats. Moreover, the infarct size was significantly smaller in the ribose treated animals despite a comparable ischemic area at risk in all MI-rats. The cytokine mRNA expression after MI was significantly reduced after ribose treatment, while there were no differences regarding MMP expression. Conclusion: MI size was significantly reduced and LV function significantly improved by ribose treatment at 6 h after MI. This seemed to be based on slowing the velocity of the necrotic wave front across the LV wall after MI resulting in smaller infarcts.
Fil: González, Germán Esteban. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rabald, Steffen. University of Leipzig; Alemania
Fil: Briest, Wilfried. University of Leipzig; Alemania
Fil: Gelpi, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; Argentina
Fil: Seropian, Ignacio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; Argentina
Fil: Zimmer, Heinz Gerd. University of Leipzig; Alemania
Fil: Deten, Alexander. University of Leipzig; Alemania
Materia
myocardial infarction
ribose
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102484
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102484
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in RatsGonzález, Germán EstebanRabald, SteffenBriest, WilfriedGelpi, Ricardo JorgeSeropian, Ignacio MiguelZimmer, Heinz GerdDeten, Alexandermyocardial infarctionribosehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Objective: In this study the effect of ribose on heart function and infarct-size was analyzed 6 h after myocardial infarction (MI) in rats. Methods: Continuous i.v.-infusion of NaCl or ribose (200 mg/ kg/h) was started one day prior to induction of MI in female Sprague-Dawley rats which was done by ligation of the left coronary artery. Six hours after MI heart function was measured with 3F tip catheter, cardiac output by thermodilution method. Thereafter the ischemic area was delineated by Evans Blue infusion, and the infarct area was visualized by triphenyltetrazolium chloride staining. The mRNA expression of interleukin (IL)-1β, IL-6, matrixmetalloproteinase (MMP)-8, and -9 was measured by ribonuclease protection assay. Results: Heart function was severely depressed 6 hours after coronary artery occlusion, but recovered significantly under the influence of ribose. Left ventricular (LV) systolic pressure (LVSP) and contractility (LVdP/dtmax) were restored to the normal levels of sham-operated animals, while parameters of LV relaxation (LVdP/dtmin and time constant of relaxation τ) were impaired compared to sham-operated animals, but significantly improved by ribose treatment compared to shamtreated MI-rats. Moreover, the infarct size was significantly smaller in the ribose treated animals despite a comparable ischemic area at risk in all MI-rats. The cytokine mRNA expression after MI was significantly reduced after ribose treatment, while there were no differences regarding MMP expression. Conclusion: MI size was significantly reduced and LV function significantly improved by ribose treatment at 6 h after MI. This seemed to be based on slowing the velocity of the necrotic wave front across the LV wall after MI resulting in smaller infarcts.Fil: González, Germán Esteban. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rabald, Steffen. University of Leipzig; AlemaniaFil: Briest, Wilfried. University of Leipzig; AlemaniaFil: Gelpi, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; ArgentinaFil: Seropian, Ignacio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; ArgentinaFil: Zimmer, Heinz Gerd. University of Leipzig; AlemaniaFil: Deten, Alexander. University of Leipzig; AlemaniaKarger2009-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102484González, Germán Esteban; Rabald, Steffen; Briest, Wilfried; Gelpi, Ricardo Jorge; Seropian, Ignacio Miguel; et al.; Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats; Karger; Cellular Physiology and Biochemistry; 24; 3-4; 8-2009; 211-2181015-8987CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000233247info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/233247info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:08Zoai:ri.conicet.gov.ar:11336/102484instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:08.83CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats
title Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats
spellingShingle Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats
González, Germán Esteban
myocardial infarction
ribose
title_short Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats
title_full Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats
title_fullStr Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats
title_full_unstemmed Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats
title_sort Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats
dc.creator.none.fl_str_mv González, Germán Esteban
Rabald, Steffen
Briest, Wilfried
Gelpi, Ricardo Jorge
Seropian, Ignacio Miguel
Zimmer, Heinz Gerd
Deten, Alexander
author González, Germán Esteban
author_facet González, Germán Esteban
Rabald, Steffen
Briest, Wilfried
Gelpi, Ricardo Jorge
Seropian, Ignacio Miguel
Zimmer, Heinz Gerd
Deten, Alexander
author_role author
author2 Rabald, Steffen
Briest, Wilfried
Gelpi, Ricardo Jorge
Seropian, Ignacio Miguel
Zimmer, Heinz Gerd
Deten, Alexander
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv myocardial infarction
ribose
topic myocardial infarction
ribose
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: In this study the effect of ribose on heart function and infarct-size was analyzed 6 h after myocardial infarction (MI) in rats. Methods: Continuous i.v.-infusion of NaCl or ribose (200 mg/ kg/h) was started one day prior to induction of MI in female Sprague-Dawley rats which was done by ligation of the left coronary artery. Six hours after MI heart function was measured with 3F tip catheter, cardiac output by thermodilution method. Thereafter the ischemic area was delineated by Evans Blue infusion, and the infarct area was visualized by triphenyltetrazolium chloride staining. The mRNA expression of interleukin (IL)-1β, IL-6, matrixmetalloproteinase (MMP)-8, and -9 was measured by ribonuclease protection assay. Results: Heart function was severely depressed 6 hours after coronary artery occlusion, but recovered significantly under the influence of ribose. Left ventricular (LV) systolic pressure (LVSP) and contractility (LVdP/dtmax) were restored to the normal levels of sham-operated animals, while parameters of LV relaxation (LVdP/dtmin and time constant of relaxation τ) were impaired compared to sham-operated animals, but significantly improved by ribose treatment compared to shamtreated MI-rats. Moreover, the infarct size was significantly smaller in the ribose treated animals despite a comparable ischemic area at risk in all MI-rats. The cytokine mRNA expression after MI was significantly reduced after ribose treatment, while there were no differences regarding MMP expression. Conclusion: MI size was significantly reduced and LV function significantly improved by ribose treatment at 6 h after MI. This seemed to be based on slowing the velocity of the necrotic wave front across the LV wall after MI resulting in smaller infarcts.
Fil: González, Germán Esteban. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rabald, Steffen. University of Leipzig; Alemania
Fil: Briest, Wilfried. University of Leipzig; Alemania
Fil: Gelpi, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; Argentina
Fil: Seropian, Ignacio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiopatología Cardiovascular; Argentina
Fil: Zimmer, Heinz Gerd. University of Leipzig; Alemania
Fil: Deten, Alexander. University of Leipzig; Alemania
description Objective: In this study the effect of ribose on heart function and infarct-size was analyzed 6 h after myocardial infarction (MI) in rats. Methods: Continuous i.v.-infusion of NaCl or ribose (200 mg/ kg/h) was started one day prior to induction of MI in female Sprague-Dawley rats which was done by ligation of the left coronary artery. Six hours after MI heart function was measured with 3F tip catheter, cardiac output by thermodilution method. Thereafter the ischemic area was delineated by Evans Blue infusion, and the infarct area was visualized by triphenyltetrazolium chloride staining. The mRNA expression of interleukin (IL)-1β, IL-6, matrixmetalloproteinase (MMP)-8, and -9 was measured by ribonuclease protection assay. Results: Heart function was severely depressed 6 hours after coronary artery occlusion, but recovered significantly under the influence of ribose. Left ventricular (LV) systolic pressure (LVSP) and contractility (LVdP/dtmax) were restored to the normal levels of sham-operated animals, while parameters of LV relaxation (LVdP/dtmin and time constant of relaxation τ) were impaired compared to sham-operated animals, but significantly improved by ribose treatment compared to shamtreated MI-rats. Moreover, the infarct size was significantly smaller in the ribose treated animals despite a comparable ischemic area at risk in all MI-rats. The cytokine mRNA expression after MI was significantly reduced after ribose treatment, while there were no differences regarding MMP expression. Conclusion: MI size was significantly reduced and LV function significantly improved by ribose treatment at 6 h after MI. This seemed to be based on slowing the velocity of the necrotic wave front across the LV wall after MI resulting in smaller infarcts.
publishDate 2009
dc.date.none.fl_str_mv 2009-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102484
González, Germán Esteban; Rabald, Steffen; Briest, Wilfried; Gelpi, Ricardo Jorge; Seropian, Ignacio Miguel; et al.; Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats; Karger; Cellular Physiology and Biochemistry; 24; 3-4; 8-2009; 211-218
1015-8987
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102484
identifier_str_mv González, Germán Esteban; Rabald, Steffen; Briest, Wilfried; Gelpi, Ricardo Jorge; Seropian, Ignacio Miguel; et al.; Ribose Treatment Reduced the Infarct Size and Improved Heart Function after Myocardial Infarction in Rats; Karger; Cellular Physiology and Biochemistry; 24; 3-4; 8-2009; 211-218
1015-8987
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000233247
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/233247
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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