CFRT is involved in colorectal cancer stem cell phenotype
- Autores
- Palma, Alejandra Graciela; Soares Machado, Mileni; Marino, Gabriela Inés; Lira, María Cecilia; Rosa, Francisco Damián; Rubio, Maria Fernanda; Costas, Monica Alejandra
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- CFTR is a chloride channel expressed in many epithelial cells and there is a relationship between this channel and cancer. The cancer stem cells (CSC) are responsible of tumorigenesis, secondary focus formation in metastasis and chemoresistance. We previously found that CFTR is expressed in colorectal cancer cells HCT116 and is associated with cancer stem cells (CSC). To further investigate this, we inhibited CFTR activity with CFTR inhibitor-172 or downregulated CFTR expression with two different shRNA (shCFTR1 or shCFTR2) in HCT116 cells. When these cells were transfected with the shCFTRs, we observed a downregulation of both CFTR protein and mRNA by Western blot, immunofluorescence and RT-PCR. Then we determined the clonogenic growth, a property of CSC, of control HCT116 cells and when CFTR is inhibited after seven days of platting at low density. The number of colonies was diminished in cells where CFTR was inhibited (p<0.05). Next we analyzed the expression of CSC markers in these cells. A lower amount of the colon cancer stem cell marker CD133, Nanog, cMyc and Oct-4 were detected by immunofluorescence staining of HCT116 cells when CFTR is inhibited. From these and our previous results we confirmed that the channel CFTR expression and activity are associated with CSC, indicating that CFTR could have an influence on the CSC properties at least in human colorectal cancer.
Fil: Palma, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Soares Machado, Mileni. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Marino, Gabriela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Lira, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Rosa, Francisco Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Rubio, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Costas, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Reunión Anual de las Sociedades de Biociencia
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Sociedad Argentina de Protozoología - Materia
-
CFTR
RAC3
COLORECTAL CANCER
CANCER STEM CELLS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/163923
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CFRT is involved in colorectal cancer stem cell phenotypePalma, Alejandra GracielaSoares Machado, MileniMarino, Gabriela InésLira, María CeciliaRosa, Francisco DamiánRubio, Maria FernandaCostas, Monica AlejandraCFTRRAC3COLORECTAL CANCERCANCER STEM CELLShttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3CFTR is a chloride channel expressed in many epithelial cells and there is a relationship between this channel and cancer. The cancer stem cells (CSC) are responsible of tumorigenesis, secondary focus formation in metastasis and chemoresistance. We previously found that CFTR is expressed in colorectal cancer cells HCT116 and is associated with cancer stem cells (CSC). To further investigate this, we inhibited CFTR activity with CFTR inhibitor-172 or downregulated CFTR expression with two different shRNA (shCFTR1 or shCFTR2) in HCT116 cells. When these cells were transfected with the shCFTRs, we observed a downregulation of both CFTR protein and mRNA by Western blot, immunofluorescence and RT-PCR. Then we determined the clonogenic growth, a property of CSC, of control HCT116 cells and when CFTR is inhibited after seven days of platting at low density. The number of colonies was diminished in cells where CFTR was inhibited (p<0.05). Next we analyzed the expression of CSC markers in these cells. A lower amount of the colon cancer stem cell marker CD133, Nanog, cMyc and Oct-4 were detected by immunofluorescence staining of HCT116 cells when CFTR is inhibited. From these and our previous results we confirmed that the channel CFTR expression and activity are associated with CSC, indicating that CFTR could have an influence on the CSC properties at least in human colorectal cancer.Fil: Palma, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Soares Machado, Mileni. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Marino, Gabriela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Lira, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Rosa, Francisco Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Rubio, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Costas, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaReunión Anual de las Sociedades de BiocienciaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioSociedad Argentina de ProtozoologíaFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163923CFRT is involved in colorectal cancer stem cell phenotype; Reunión Anual de las Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 246-2460025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:32Zoai:ri.conicet.gov.ar:11336/163923instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:32.316CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
CFRT is involved in colorectal cancer stem cell phenotype |
title |
CFRT is involved in colorectal cancer stem cell phenotype |
spellingShingle |
CFRT is involved in colorectal cancer stem cell phenotype Palma, Alejandra Graciela CFTR RAC3 COLORECTAL CANCER CANCER STEM CELLS |
title_short |
CFRT is involved in colorectal cancer stem cell phenotype |
title_full |
CFRT is involved in colorectal cancer stem cell phenotype |
title_fullStr |
CFRT is involved in colorectal cancer stem cell phenotype |
title_full_unstemmed |
CFRT is involved in colorectal cancer stem cell phenotype |
title_sort |
CFRT is involved in colorectal cancer stem cell phenotype |
dc.creator.none.fl_str_mv |
Palma, Alejandra Graciela Soares Machado, Mileni Marino, Gabriela Inés Lira, María Cecilia Rosa, Francisco Damián Rubio, Maria Fernanda Costas, Monica Alejandra |
author |
Palma, Alejandra Graciela |
author_facet |
Palma, Alejandra Graciela Soares Machado, Mileni Marino, Gabriela Inés Lira, María Cecilia Rosa, Francisco Damián Rubio, Maria Fernanda Costas, Monica Alejandra |
author_role |
author |
author2 |
Soares Machado, Mileni Marino, Gabriela Inés Lira, María Cecilia Rosa, Francisco Damián Rubio, Maria Fernanda Costas, Monica Alejandra |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
CFTR RAC3 COLORECTAL CANCER CANCER STEM CELLS |
topic |
CFTR RAC3 COLORECTAL CANCER CANCER STEM CELLS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.5 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
CFTR is a chloride channel expressed in many epithelial cells and there is a relationship between this channel and cancer. The cancer stem cells (CSC) are responsible of tumorigenesis, secondary focus formation in metastasis and chemoresistance. We previously found that CFTR is expressed in colorectal cancer cells HCT116 and is associated with cancer stem cells (CSC). To further investigate this, we inhibited CFTR activity with CFTR inhibitor-172 or downregulated CFTR expression with two different shRNA (shCFTR1 or shCFTR2) in HCT116 cells. When these cells were transfected with the shCFTRs, we observed a downregulation of both CFTR protein and mRNA by Western blot, immunofluorescence and RT-PCR. Then we determined the clonogenic growth, a property of CSC, of control HCT116 cells and when CFTR is inhibited after seven days of platting at low density. The number of colonies was diminished in cells where CFTR was inhibited (p<0.05). Next we analyzed the expression of CSC markers in these cells. A lower amount of the colon cancer stem cell marker CD133, Nanog, cMyc and Oct-4 were detected by immunofluorescence staining of HCT116 cells when CFTR is inhibited. From these and our previous results we confirmed that the channel CFTR expression and activity are associated with CSC, indicating that CFTR could have an influence on the CSC properties at least in human colorectal cancer. Fil: Palma, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Soares Machado, Mileni. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Marino, Gabriela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Lira, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Rosa, Francisco Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Rubio, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Costas, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Reunión Anual de las Sociedades de Biociencia Mar del Plata Argentina Sociedad Argentina de Investigación Clínica Asociación Argentina de Farmacología Experimental Sociedad Argentina de Biología Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio Sociedad Argentina de Protozoología |
description |
CFTR is a chloride channel expressed in many epithelial cells and there is a relationship between this channel and cancer. The cancer stem cells (CSC) are responsible of tumorigenesis, secondary focus formation in metastasis and chemoresistance. We previously found that CFTR is expressed in colorectal cancer cells HCT116 and is associated with cancer stem cells (CSC). To further investigate this, we inhibited CFTR activity with CFTR inhibitor-172 or downregulated CFTR expression with two different shRNA (shCFTR1 or shCFTR2) in HCT116 cells. When these cells were transfected with the shCFTRs, we observed a downregulation of both CFTR protein and mRNA by Western blot, immunofluorescence and RT-PCR. Then we determined the clonogenic growth, a property of CSC, of control HCT116 cells and when CFTR is inhibited after seven days of platting at low density. The number of colonies was diminished in cells where CFTR was inhibited (p<0.05). Next we analyzed the expression of CSC markers in these cells. A lower amount of the colon cancer stem cell marker CD133, Nanog, cMyc and Oct-4 were detected by immunofluorescence staining of HCT116 cells when CFTR is inhibited. From these and our previous results we confirmed that the channel CFTR expression and activity are associated with CSC, indicating that CFTR could have an influence on the CSC properties at least in human colorectal cancer. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/163923 CFRT is involved in colorectal cancer stem cell phenotype; Reunión Anual de las Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 246-246 0025-7680 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/163923 |
identifier_str_mv |
CFRT is involved in colorectal cancer stem cell phenotype; Reunión Anual de las Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 246-246 0025-7680 1669-9106 CONICET Digital CONICET |
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eng |
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Fundación Revista Medicina |
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Fundación Revista Medicina |
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