Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers

Autores
Coria, Maria Sumampa; Reineri, Pablo Sebastian; Pighín, Darío Gabriel; Barrionuevo, María Guadalupe del Valle; Carranza, Pedro Gabriel; Grigioni, Gabriela Maria; Palma, Gustavo Adolfo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.
Fil: Coria, Maria Sumampa. Universidad Nacional de Santiago del Estero; Argentina. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Reineri, Pablo Sebastian. Universidad Nacional de Santiago del Estero; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Pighín, Darío Gabriel. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad de Morón; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrionuevo, María Guadalupe del Valle. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Carranza, Pedro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Grigioni, Gabriela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad de Morón; Argentina
Fil: Palma, Gustavo Adolfo. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Materia
BEEF CATTLE
CALPAIN SYSTEM
CATTLE FEEDING
MEAT QUALITY
MEAT TENDERNESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/148550
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/148550
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steersCoria, Maria SumampaReineri, Pablo SebastianPighín, Darío GabrielBarrionuevo, María Guadalupe del ValleCarranza, Pedro GabrielGrigioni, Gabriela MariaPalma, Gustavo AdolfoBEEF CATTLECALPAIN SYSTEMCATTLE FEEDINGMEAT QUALITYMEAT TENDERNESShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.Fil: Coria, Maria Sumampa. Universidad Nacional de Santiago del Estero; Argentina. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Reineri, Pablo Sebastian. Universidad Nacional de Santiago del Estero; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Pighín, Darío Gabriel. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad de Morón; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barrionuevo, María Guadalupe del Valle. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Carranza, Pedro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Grigioni, Gabriela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad de Morón; ArgentinaFil: Palma, Gustavo Adolfo. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaAsian-australasian Assoc Animal Production Soc2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/148550Coria, Maria Sumampa; Reineri, Pablo Sebastian; Pighín, Darío Gabriel; Barrionuevo, María Guadalupe del Valle; Carranza, Pedro Gabriel; et al.; Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers; Asian-australasian Assoc Animal Production Soc; Asian-australasian Journal Of Animal Sciences; 33; 5; 8-2019; 753-7621011-2367CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ajas.info/journal/view.php?doi=10.5713/ajas.19.0163info:eu-repo/semantics/altIdentifier/doi/10.5713/ajas.19.0163info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:09Zoai:ri.conicet.gov.ar:11336/148550instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:09.425CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
title Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
spellingShingle Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
Coria, Maria Sumampa
BEEF CATTLE
CALPAIN SYSTEM
CATTLE FEEDING
MEAT QUALITY
MEAT TENDERNESS
title_short Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
title_full Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
title_fullStr Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
title_full_unstemmed Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
title_sort Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
dc.creator.none.fl_str_mv Coria, Maria Sumampa
Reineri, Pablo Sebastian
Pighín, Darío Gabriel
Barrionuevo, María Guadalupe del Valle
Carranza, Pedro Gabriel
Grigioni, Gabriela Maria
Palma, Gustavo Adolfo
author Coria, Maria Sumampa
author_facet Coria, Maria Sumampa
Reineri, Pablo Sebastian
Pighín, Darío Gabriel
Barrionuevo, María Guadalupe del Valle
Carranza, Pedro Gabriel
Grigioni, Gabriela Maria
Palma, Gustavo Adolfo
author_role author
author2 Reineri, Pablo Sebastian
Pighín, Darío Gabriel
Barrionuevo, María Guadalupe del Valle
Carranza, Pedro Gabriel
Grigioni, Gabriela Maria
Palma, Gustavo Adolfo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BEEF CATTLE
CALPAIN SYSTEM
CATTLE FEEDING
MEAT QUALITY
MEAT TENDERNESS
topic BEEF CATTLE
CALPAIN SYSTEM
CATTLE FEEDING
MEAT QUALITY
MEAT TENDERNESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.
Fil: Coria, Maria Sumampa. Universidad Nacional de Santiago del Estero; Argentina. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Reineri, Pablo Sebastian. Universidad Nacional de Santiago del Estero; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Pighín, Darío Gabriel. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad de Morón; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrionuevo, María Guadalupe del Valle. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Carranza, Pedro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Grigioni, Gabriela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad de Morón; Argentina
Fil: Palma, Gustavo Adolfo. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
description Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/148550
Coria, Maria Sumampa; Reineri, Pablo Sebastian; Pighín, Darío Gabriel; Barrionuevo, María Guadalupe del Valle; Carranza, Pedro Gabriel; et al.; Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers; Asian-australasian Assoc Animal Production Soc; Asian-australasian Journal Of Animal Sciences; 33; 5; 8-2019; 753-762
1011-2367
CONICET Digital
CONICET
url http://hdl.handle.net/11336/148550
identifier_str_mv Coria, Maria Sumampa; Reineri, Pablo Sebastian; Pighín, Darío Gabriel; Barrionuevo, María Guadalupe del Valle; Carranza, Pedro Gabriel; et al.; Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers; Asian-australasian Assoc Animal Production Soc; Asian-australasian Journal Of Animal Sciences; 33; 5; 8-2019; 753-762
1011-2367
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ajas.info/journal/view.php?doi=10.5713/ajas.19.0163
info:eu-repo/semantics/altIdentifier/doi/10.5713/ajas.19.0163
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asian-australasian Assoc Animal Production Soc
publisher.none.fl_str_mv Asian-australasian Assoc Animal Production Soc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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