Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers

Autores
Coria, María Sumampa; Reineri, Pablo Sebastian; Pighin, Darío Gabriel; Barrionuevo, María Guadalupe; Carranza, Pedro Gabriel; Grigioni, Gabriela Maria; Palma, Gustavo Adolfo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.
Fil: Coria, María Sumampa. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.
Fil: Reineri, Pablo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero. Campo Experimental Francisco Cantos; Argentina.
Fil: Pighin, Darío Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Universidad de Morón; Argentina.
Fil: Barrionuevo, María Guadalupe. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Carranza, Pedro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Transferencia Santiago del Estero; Argentina.
Fil: Grigioni, Gabriela Maria. Consejo Nacional de Investigaciones Cientiíficas y Ténicas; Argentina.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Universidad de Morón; Argentina.
Fil: Palma, Gustavo Adolfo. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.
Fuente
Asian-Australasian journal animal sciences 12 (8) :1171-1176. (2019)
Materia
Beef Cattle
Cattle Feeding
Calpain
Meat Quality
Tenderness
Ganado de Carne
Alimentación de Ganado Vacuno
Calpaina
Calidad de la Carne
Ternura
Calpain System
Meat Tenderness
Sistema de Calpaína
Terneza de la Carne
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6913
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oai_identifier_str oai:localhost:20.500.12123/6913
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steersCoria, María SumampaReineri, Pablo SebastianPighin, Darío GabrielBarrionuevo, María GuadalupeCarranza, Pedro GabrielGrigioni, Gabriela MariaPalma, Gustavo AdolfoBeef CattleCattle FeedingCalpainMeat QualityTendernessGanado de CarneAlimentación de Ganado VacunoCalpainaCalidad de la CarneTernuraCalpain SystemMeat TendernessSistema de CalpaínaTerneza de la CarneObjective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.Fil: Coria, María Sumampa. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.Fil: Reineri, Pablo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero. Campo Experimental Francisco Cantos; Argentina.Fil: Pighin, Darío Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Universidad de Morón; Argentina.Fil: Barrionuevo, María Guadalupe. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Carranza, Pedro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Transferencia Santiago del Estero; Argentina.Fil: Grigioni, Gabriela Maria. Consejo Nacional de Investigaciones Cientiíficas y Ténicas; Argentina.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Universidad de Morón; Argentina.Fil: Palma, Gustavo Adolfo. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.Asian-Australasian Association of Animal Production Societies (AAAP)2020-03-10T14:33:10Z2020-03-10T14:33:10Z2019-08-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6913https://www.ajas.info/current/index.php?vol=12&no=8&special=1011-23671976-5517 (Online)https://doi.org/10.5713/ajas.19.0163Asian-Australasian journal animal sciences 12 (8) :1171-1176. (2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:45Zoai:localhost:20.500.12123/6913instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:46.182INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
title Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
spellingShingle Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
Coria, María Sumampa
Beef Cattle
Cattle Feeding
Calpain
Meat Quality
Tenderness
Ganado de Carne
Alimentación de Ganado Vacuno
Calpaina
Calidad de la Carne
Ternura
Calpain System
Meat Tenderness
Sistema de Calpaína
Terneza de la Carne
title_short Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
title_full Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
title_fullStr Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
title_full_unstemmed Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
title_sort Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers
dc.creator.none.fl_str_mv Coria, María Sumampa
Reineri, Pablo Sebastian
Pighin, Darío Gabriel
Barrionuevo, María Guadalupe
Carranza, Pedro Gabriel
Grigioni, Gabriela Maria
Palma, Gustavo Adolfo
author Coria, María Sumampa
author_facet Coria, María Sumampa
Reineri, Pablo Sebastian
Pighin, Darío Gabriel
Barrionuevo, María Guadalupe
Carranza, Pedro Gabriel
Grigioni, Gabriela Maria
Palma, Gustavo Adolfo
author_role author
author2 Reineri, Pablo Sebastian
Pighin, Darío Gabriel
Barrionuevo, María Guadalupe
Carranza, Pedro Gabriel
Grigioni, Gabriela Maria
Palma, Gustavo Adolfo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Beef Cattle
Cattle Feeding
Calpain
Meat Quality
Tenderness
Ganado de Carne
Alimentación de Ganado Vacuno
Calpaina
Calidad de la Carne
Ternura
Calpain System
Meat Tenderness
Sistema de Calpaína
Terneza de la Carne
topic Beef Cattle
Cattle Feeding
Calpain
Meat Quality
Tenderness
Ganado de Carne
Alimentación de Ganado Vacuno
Calpaina
Calidad de la Carne
Ternura
Calpain System
Meat Tenderness
Sistema de Calpaína
Terneza de la Carne
dc.description.none.fl_txt_mv Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.
Fil: Coria, María Sumampa. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.
Fil: Reineri, Pablo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero. Campo Experimental Francisco Cantos; Argentina.
Fil: Pighin, Darío Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Universidad de Morón; Argentina.
Fil: Barrionuevo, María Guadalupe. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Carranza, Pedro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Transferencia Santiago del Estero; Argentina.
Fil: Grigioni, Gabriela Maria. Consejo Nacional de Investigaciones Cientiíficas y Ténicas; Argentina.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Universidad de Morón; Argentina.
Fil: Palma, Gustavo Adolfo. Instituto de Bionanotecnología del NOA. Laboratorio de Producción y Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.
description Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-03
2020-03-10T14:33:10Z
2020-03-10T14:33:10Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6913
https://www.ajas.info/current/index.php?vol=12&no=8&special=
1011-2367
1976-5517 (Online)
https://doi.org/10.5713/ajas.19.0163
url http://hdl.handle.net/20.500.12123/6913
https://www.ajas.info/current/index.php?vol=12&no=8&special=
https://doi.org/10.5713/ajas.19.0163
identifier_str_mv 1011-2367
1976-5517 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Asian-Australasian Association of Animal Production Societies (AAAP)
publisher.none.fl_str_mv Asian-Australasian Association of Animal Production Societies (AAAP)
dc.source.none.fl_str_mv Asian-Australasian journal animal sciences 12 (8) :1171-1176. (2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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