Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium

Autores
Almeyda, D.; Scodelaro Bilbao, Paola Gabriela; Constenla, Diana Teresita; Popovich, Cecilia Angelines; Leonardi, Patricia Ines
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fish oil is widely used as a source of essential long chain poly-unsaturated omega-3 fatty acids, such eicosapentaenoic (EPA) and docosahexaenoic (DHA), for aquaculture. However, there is increasing interest in reducing the aquaculture industry?s dependence on this resource due to its unsustainability and variable cost and supply. Marine microalgae naturally produce EPA and DHA fatty acids and they content can be modified by manipulation of the growth conditions. The aim of this study was to assess the effect of temperature on fatty acid composition of the marine diatom Cylindrotheca closterium. To that end, C. closterium was grown in a photobioreactor: a) at 20 °C (control) and b) lowering the temperature from 20°C to 11°C in the stationary growth phase. Total lipid content and lipid fractions were determined spectrophotometrically and gravimetrically. Gas chromatography was performed to analyze fatty acid composition. C. closterium growth was not affected by temperature variation, as showed by cell density and dry weight determinations. When the temperature was lowered, triacylglyceride content significantly increased compared to the control condition. In addition, among omega-3 fatty acids, DHA and EPA showed a marked increase. Thus, these results evidence the potential of this strain as an alternative and sustainable source for aquaculture purposes.
Fil: Almeyda, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Paraná
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Materia
FATTYACIDS
CYLINDROTHECA
TEMPERATURE
DIATOM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/129546
Acceder
id CONICETDig_a7052f79a3f6938204d68892b2ef9eef
oai_identifier_str oai:ri.conicet.gov.ar:11336/129546
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closteriumAlmeyda, D.Scodelaro Bilbao, Paola GabrielaConstenla, Diana TeresitaPopovich, Cecilia AngelinesLeonardi, Patricia InesFATTYACIDSCYLINDROTHECATEMPERATUREDIATOMhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Fish oil is widely used as a source of essential long chain poly-unsaturated omega-3 fatty acids, such eicosapentaenoic (EPA) and docosahexaenoic (DHA), for aquaculture. However, there is increasing interest in reducing the aquaculture industry?s dependence on this resource due to its unsustainability and variable cost and supply. Marine microalgae naturally produce EPA and DHA fatty acids and they content can be modified by manipulation of the growth conditions. The aim of this study was to assess the effect of temperature on fatty acid composition of the marine diatom Cylindrotheca closterium. To that end, C. closterium was grown in a photobioreactor: a) at 20 °C (control) and b) lowering the temperature from 20°C to 11°C in the stationary growth phase. Total lipid content and lipid fractions were determined spectrophotometrically and gravimetrically. Gas chromatography was performed to analyze fatty acid composition. C. closterium growth was not affected by temperature variation, as showed by cell density and dry weight determinations. When the temperature was lowered, triacylglyceride content significantly increased compared to the control condition. In addition, among omega-3 fatty acids, DHA and EPA showed a marked increase. Thus, these results evidence the potential of this strain as an alternative and sustainable source for aquaculture purposes.Fil: Almeyda, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularParanáArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularSociedad Argentina de Investigación Bioquímica y Biología Molecular2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/129546Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 1-11667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2018.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:17Zoai:ri.conicet.gov.ar:11336/129546instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:18.238CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium
title Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium
spellingShingle Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium
Almeyda, D.
FATTYACIDS
CYLINDROTHECA
TEMPERATURE
DIATOM
title_short Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium
title_full Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium
title_fullStr Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium
title_full_unstemmed Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium
title_sort Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium
dc.creator.none.fl_str_mv Almeyda, D.
Scodelaro Bilbao, Paola Gabriela
Constenla, Diana Teresita
Popovich, Cecilia Angelines
Leonardi, Patricia Ines
author Almeyda, D.
author_facet Almeyda, D.
Scodelaro Bilbao, Paola Gabriela
Constenla, Diana Teresita
Popovich, Cecilia Angelines
Leonardi, Patricia Ines
author_role author
author2 Scodelaro Bilbao, Paola Gabriela
Constenla, Diana Teresita
Popovich, Cecilia Angelines
Leonardi, Patricia Ines
author2_role author
author
author
author
dc.subject.none.fl_str_mv FATTYACIDS
CYLINDROTHECA
TEMPERATURE
DIATOM
topic FATTYACIDS
CYLINDROTHECA
TEMPERATURE
DIATOM
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Fish oil is widely used as a source of essential long chain poly-unsaturated omega-3 fatty acids, such eicosapentaenoic (EPA) and docosahexaenoic (DHA), for aquaculture. However, there is increasing interest in reducing the aquaculture industry?s dependence on this resource due to its unsustainability and variable cost and supply. Marine microalgae naturally produce EPA and DHA fatty acids and they content can be modified by manipulation of the growth conditions. The aim of this study was to assess the effect of temperature on fatty acid composition of the marine diatom Cylindrotheca closterium. To that end, C. closterium was grown in a photobioreactor: a) at 20 °C (control) and b) lowering the temperature from 20°C to 11°C in the stationary growth phase. Total lipid content and lipid fractions were determined spectrophotometrically and gravimetrically. Gas chromatography was performed to analyze fatty acid composition. C. closterium growth was not affected by temperature variation, as showed by cell density and dry weight determinations. When the temperature was lowered, triacylglyceride content significantly increased compared to the control condition. In addition, among omega-3 fatty acids, DHA and EPA showed a marked increase. Thus, these results evidence the potential of this strain as an alternative and sustainable source for aquaculture purposes.
Fil: Almeyda, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Scodelaro Bilbao, Paola Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Paraná
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
description Fish oil is widely used as a source of essential long chain poly-unsaturated omega-3 fatty acids, such eicosapentaenoic (EPA) and docosahexaenoic (DHA), for aquaculture. However, there is increasing interest in reducing the aquaculture industry?s dependence on this resource due to its unsustainability and variable cost and supply. Marine microalgae naturally produce EPA and DHA fatty acids and they content can be modified by manipulation of the growth conditions. The aim of this study was to assess the effect of temperature on fatty acid composition of the marine diatom Cylindrotheca closterium. To that end, C. closterium was grown in a photobioreactor: a) at 20 °C (control) and b) lowering the temperature from 20°C to 11°C in the stationary growth phase. Total lipid content and lipid fractions were determined spectrophotometrically and gravimetrically. Gas chromatography was performed to analyze fatty acid composition. C. closterium growth was not affected by temperature variation, as showed by cell density and dry weight determinations. When the temperature was lowered, triacylglyceride content significantly increased compared to the control condition. In addition, among omega-3 fatty acids, DHA and EPA showed a marked increase. Thus, these results evidence the potential of this strain as an alternative and sustainable source for aquaculture purposes.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/129546
Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 1-1
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/129546
identifier_str_mv Effect of culture temperature on fatty acid composition of diatom Cylindrotheca closterium; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 1-1
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2018.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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