Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion

Autores
Coronel, Maria Florencia; Defagot, Maria C.; Musolino, Patricia L.; Villar, Marcelo Jose
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Recent studies have analyzed the role of nitric oxide (NO) in pain modulation in several models of sciatic nerve injury. In the present study we have investigated NO production in lumbar dorsal root ganglia (DRG) and spinal cord (SC) over time after sciatic nerve cut. Neuronal nitric oxide synthase (nNOS)-like immunoreactivity (LI) was also determined, since the expression and activity of the enzyme do not always correlate. Nerve section induced a progressive increase in NO production in the ipsilateral L4-5 DRGs and the corresponding levels in the SC in a pattern that correlated with nNOS-LI; this increase was gradual after 7 days of survival time, more pronounced after 14 days, with the highest values detected 28 days after axotomy. This peak was followed by a progressive decrease, reaching control values 42 days after the lesion. The present study shows that nNOS upregulation is related to an increased NO production and release. The temporal pattern of NO production parallels the one observed for the expression of the enzyme, suggesting that the induction of nNOS synthesis yields a protein that is functional and highly active.
Fil: Coronel, Maria Florencia. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Defagot, Maria C.. Universidad Austral; Argentina
Fil: Musolino, Patricia L.. Universidad Austral; Argentina
Fil: Villar, Marcelo Jose. Universidad Austral; Argentina
Materia
Primary Afferent Neurons
Neurophatic Pain
Neuronal Nitric Oxide Synthase
Sciatic Nerve Injury
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31470
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31470
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network_name_str CONICET Digital (CONICET)
spelling Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesionCoronel, Maria FlorenciaDefagot, Maria C.Musolino, Patricia L.Villar, Marcelo JosePrimary Afferent NeuronsNeurophatic PainNeuronal Nitric Oxide SynthaseSciatic Nerve Injuryhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Recent studies have analyzed the role of nitric oxide (NO) in pain modulation in several models of sciatic nerve injury. In the present study we have investigated NO production in lumbar dorsal root ganglia (DRG) and spinal cord (SC) over time after sciatic nerve cut. Neuronal nitric oxide synthase (nNOS)-like immunoreactivity (LI) was also determined, since the expression and activity of the enzyme do not always correlate. Nerve section induced a progressive increase in NO production in the ipsilateral L4-5 DRGs and the corresponding levels in the SC in a pattern that correlated with nNOS-LI; this increase was gradual after 7 days of survival time, more pronounced after 14 days, with the highest values detected 28 days after axotomy. This peak was followed by a progressive decrease, reaching control values 42 days after the lesion. The present study shows that nNOS upregulation is related to an increased NO production and release. The temporal pattern of NO production parallels the one observed for the expression of the enzyme, suggesting that the induction of nNOS synthesis yields a protein that is functional and highly active.Fil: Coronel, Maria Florencia. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Defagot, Maria C.. Universidad Austral; ArgentinaFil: Musolino, Patricia L.. Universidad Austral; ArgentinaFil: Villar, Marcelo Jose. Universidad Austral; ArgentinaThe Haworth Medical Press2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31470Villar, Marcelo Jose; Musolino, Patricia L.; Defagot, Maria C.; Coronel, Maria Florencia; Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion; The Haworth Medical Press; Journal of Neuropathic Pain and Symptom Palliation; 1; 2; 2005; 3-91543-7698CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/J426v01n02_02info:eu-repo/semantics/altIdentifier/doi/10.3109/J426v01n02_02info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:31Zoai:ri.conicet.gov.ar:11336/31470instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:31.489CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion
title Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion
spellingShingle Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion
Coronel, Maria Florencia
Primary Afferent Neurons
Neurophatic Pain
Neuronal Nitric Oxide Synthase
Sciatic Nerve Injury
title_short Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion
title_full Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion
title_fullStr Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion
title_full_unstemmed Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion
title_sort Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion
dc.creator.none.fl_str_mv Coronel, Maria Florencia
Defagot, Maria C.
Musolino, Patricia L.
Villar, Marcelo Jose
author Coronel, Maria Florencia
author_facet Coronel, Maria Florencia
Defagot, Maria C.
Musolino, Patricia L.
Villar, Marcelo Jose
author_role author
author2 Defagot, Maria C.
Musolino, Patricia L.
Villar, Marcelo Jose
author2_role author
author
author
dc.subject.none.fl_str_mv Primary Afferent Neurons
Neurophatic Pain
Neuronal Nitric Oxide Synthase
Sciatic Nerve Injury
topic Primary Afferent Neurons
Neurophatic Pain
Neuronal Nitric Oxide Synthase
Sciatic Nerve Injury
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Recent studies have analyzed the role of nitric oxide (NO) in pain modulation in several models of sciatic nerve injury. In the present study we have investigated NO production in lumbar dorsal root ganglia (DRG) and spinal cord (SC) over time after sciatic nerve cut. Neuronal nitric oxide synthase (nNOS)-like immunoreactivity (LI) was also determined, since the expression and activity of the enzyme do not always correlate. Nerve section induced a progressive increase in NO production in the ipsilateral L4-5 DRGs and the corresponding levels in the SC in a pattern that correlated with nNOS-LI; this increase was gradual after 7 days of survival time, more pronounced after 14 days, with the highest values detected 28 days after axotomy. This peak was followed by a progressive decrease, reaching control values 42 days after the lesion. The present study shows that nNOS upregulation is related to an increased NO production and release. The temporal pattern of NO production parallels the one observed for the expression of the enzyme, suggesting that the induction of nNOS synthesis yields a protein that is functional and highly active.
Fil: Coronel, Maria Florencia. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Defagot, Maria C.. Universidad Austral; Argentina
Fil: Musolino, Patricia L.. Universidad Austral; Argentina
Fil: Villar, Marcelo Jose. Universidad Austral; Argentina
description Recent studies have analyzed the role of nitric oxide (NO) in pain modulation in several models of sciatic nerve injury. In the present study we have investigated NO production in lumbar dorsal root ganglia (DRG) and spinal cord (SC) over time after sciatic nerve cut. Neuronal nitric oxide synthase (nNOS)-like immunoreactivity (LI) was also determined, since the expression and activity of the enzyme do not always correlate. Nerve section induced a progressive increase in NO production in the ipsilateral L4-5 DRGs and the corresponding levels in the SC in a pattern that correlated with nNOS-LI; this increase was gradual after 7 days of survival time, more pronounced after 14 days, with the highest values detected 28 days after axotomy. This peak was followed by a progressive decrease, reaching control values 42 days after the lesion. The present study shows that nNOS upregulation is related to an increased NO production and release. The temporal pattern of NO production parallels the one observed for the expression of the enzyme, suggesting that the induction of nNOS synthesis yields a protein that is functional and highly active.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31470
Villar, Marcelo Jose; Musolino, Patricia L.; Defagot, Maria C.; Coronel, Maria Florencia; Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion; The Haworth Medical Press; Journal of Neuropathic Pain and Symptom Palliation; 1; 2; 2005; 3-9
1543-7698
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31470
identifier_str_mv Villar, Marcelo Jose; Musolino, Patricia L.; Defagot, Maria C.; Coronel, Maria Florencia; Nitric oxide production in rat dorsal root ganglia and spinal cord after sciatic nerve lesion; The Haworth Medical Press; Journal of Neuropathic Pain and Symptom Palliation; 1; 2; 2005; 3-9
1543-7698
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/J426v01n02_02
info:eu-repo/semantics/altIdentifier/doi/10.3109/J426v01n02_02
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv The Haworth Medical Press
publisher.none.fl_str_mv The Haworth Medical Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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