Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery

Autores
Savastano, Luis E.; Laurito, Sergio Roberto; Fitt, Marcos R.; Rasmussen, Jorge A.; Gonzalez Polo, Virginia; Patterson, Sean Ingram
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sciatic nerve injury has been used for over a century to investigate the process of nerve damage, to assess the absolute and relative capacity of the central and peripheral nervous systems to recover after axotomy, and to understand the development of chronic pain in many pathologies. Here we provide a historical review of the contributions of this experimental model to our current understanding of fundamental questions in the neurosciences, and an assessment of its continuing capacity to address these and future problems. We describe the different degrees of nerve injury - neurapraxia, axonotmesis, neurotmesis - together with the consequences of selective damage to the different functional and anatomic components of this nerve. The varied techniques used to model different degrees of nerve injury and their relationship to the development of neuropathic pain states are considered. We also provide a detailed anatomical description of the sciatic nerve from the spinal cord to the peripheral branches in the leg. A standardized protocol for carrying out sciatic nerve axotomy is proposed, with guides to assist in the accurate and reliable dissection of the peripheral and central branches of the nerve. Functional, histological, and biochemical criteria for the validation of the injury are described. Thus, this paper provides a review of the principal features of sciatic nerve injury, presents detailed neuroanatomical descriptions of the rat´s inferior limb and spine, compares different modes of injury, offers material for training purposes, and summarizes the immediate and longterm consequences of damage to the sciatic nerve.
Fil: Savastano, Luis E.. Universidad Nacional de Cuyo; Argentina
Fil: Laurito, Sergio Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Fitt, Marcos R.. Universidad Nacional de Cuyo; Argentina
Fil: Rasmussen, Jorge A.. Universidad Nacional de Cuyo; Argentina
Fil: Gonzalez Polo, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Patterson, Sean Ingram. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
Axotomy
Conditioning Lesion
Dorsal Root Ganglion
Sciatic Nerve Injury
Neuropathic Pain
Sensory Neuron
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32044
Acceder
id CONICETDig_0f9e7ba0b13225e46f3913040b906135
oai_identifier_str oai:ri.conicet.gov.ar:11336/32044
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recoverySavastano, Luis E.Laurito, Sergio RobertoFitt, Marcos R.Rasmussen, Jorge A.Gonzalez Polo, VirginiaPatterson, Sean IngramAxotomyConditioning LesionDorsal Root GanglionSciatic Nerve InjuryNeuropathic PainSensory Neuronhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sciatic nerve injury has been used for over a century to investigate the process of nerve damage, to assess the absolute and relative capacity of the central and peripheral nervous systems to recover after axotomy, and to understand the development of chronic pain in many pathologies. Here we provide a historical review of the contributions of this experimental model to our current understanding of fundamental questions in the neurosciences, and an assessment of its continuing capacity to address these and future problems. We describe the different degrees of nerve injury - neurapraxia, axonotmesis, neurotmesis - together with the consequences of selective damage to the different functional and anatomic components of this nerve. The varied techniques used to model different degrees of nerve injury and their relationship to the development of neuropathic pain states are considered. We also provide a detailed anatomical description of the sciatic nerve from the spinal cord to the peripheral branches in the leg. A standardized protocol for carrying out sciatic nerve axotomy is proposed, with guides to assist in the accurate and reliable dissection of the peripheral and central branches of the nerve. Functional, histological, and biochemical criteria for the validation of the injury are described. Thus, this paper provides a review of the principal features of sciatic nerve injury, presents detailed neuroanatomical descriptions of the rat´s inferior limb and spine, compares different modes of injury, offers material for training purposes, and summarizes the immediate and longterm consequences of damage to the sciatic nerve.Fil: Savastano, Luis E.. Universidad Nacional de Cuyo; ArgentinaFil: Laurito, Sergio Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Fitt, Marcos R.. Universidad Nacional de Cuyo; ArgentinaFil: Rasmussen, Jorge A.. Universidad Nacional de Cuyo; ArgentinaFil: Gonzalez Polo, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Patterson, Sean Ingram. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaElsevier2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32044Patterson, Sean Ingram; Gonzalez Polo, Virginia; Rasmussen, Jorge A.; Fitt, Marcos R.; Laurito, Sergio Roberto; Savastano, Luis E.; et al.; Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery; Elsevier; Journal of Neuroscience Methods; 227; 1-2014; 166-1800165-0270CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0165027014000314info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jneumeth.2014.01.020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:36Zoai:ri.conicet.gov.ar:11336/32044instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:36.556CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery
title Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery
spellingShingle Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery
Savastano, Luis E.
Axotomy
Conditioning Lesion
Dorsal Root Ganglion
Sciatic Nerve Injury
Neuropathic Pain
Sensory Neuron
title_short Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery
title_full Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery
title_fullStr Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery
title_full_unstemmed Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery
title_sort Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery
dc.creator.none.fl_str_mv Savastano, Luis E.
Laurito, Sergio Roberto
Fitt, Marcos R.
Rasmussen, Jorge A.
Gonzalez Polo, Virginia
Patterson, Sean Ingram
author Savastano, Luis E.
author_facet Savastano, Luis E.
Laurito, Sergio Roberto
Fitt, Marcos R.
Rasmussen, Jorge A.
Gonzalez Polo, Virginia
Patterson, Sean Ingram
author_role author
author2 Laurito, Sergio Roberto
Fitt, Marcos R.
Rasmussen, Jorge A.
Gonzalez Polo, Virginia
Patterson, Sean Ingram
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Axotomy
Conditioning Lesion
Dorsal Root Ganglion
Sciatic Nerve Injury
Neuropathic Pain
Sensory Neuron
topic Axotomy
Conditioning Lesion
Dorsal Root Ganglion
Sciatic Nerve Injury
Neuropathic Pain
Sensory Neuron
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sciatic nerve injury has been used for over a century to investigate the process of nerve damage, to assess the absolute and relative capacity of the central and peripheral nervous systems to recover after axotomy, and to understand the development of chronic pain in many pathologies. Here we provide a historical review of the contributions of this experimental model to our current understanding of fundamental questions in the neurosciences, and an assessment of its continuing capacity to address these and future problems. We describe the different degrees of nerve injury - neurapraxia, axonotmesis, neurotmesis - together with the consequences of selective damage to the different functional and anatomic components of this nerve. The varied techniques used to model different degrees of nerve injury and their relationship to the development of neuropathic pain states are considered. We also provide a detailed anatomical description of the sciatic nerve from the spinal cord to the peripheral branches in the leg. A standardized protocol for carrying out sciatic nerve axotomy is proposed, with guides to assist in the accurate and reliable dissection of the peripheral and central branches of the nerve. Functional, histological, and biochemical criteria for the validation of the injury are described. Thus, this paper provides a review of the principal features of sciatic nerve injury, presents detailed neuroanatomical descriptions of the rat´s inferior limb and spine, compares different modes of injury, offers material for training purposes, and summarizes the immediate and longterm consequences of damage to the sciatic nerve.
Fil: Savastano, Luis E.. Universidad Nacional de Cuyo; Argentina
Fil: Laurito, Sergio Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Fitt, Marcos R.. Universidad Nacional de Cuyo; Argentina
Fil: Rasmussen, Jorge A.. Universidad Nacional de Cuyo; Argentina
Fil: Gonzalez Polo, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Patterson, Sean Ingram. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description Sciatic nerve injury has been used for over a century to investigate the process of nerve damage, to assess the absolute and relative capacity of the central and peripheral nervous systems to recover after axotomy, and to understand the development of chronic pain in many pathologies. Here we provide a historical review of the contributions of this experimental model to our current understanding of fundamental questions in the neurosciences, and an assessment of its continuing capacity to address these and future problems. We describe the different degrees of nerve injury - neurapraxia, axonotmesis, neurotmesis - together with the consequences of selective damage to the different functional and anatomic components of this nerve. The varied techniques used to model different degrees of nerve injury and their relationship to the development of neuropathic pain states are considered. We also provide a detailed anatomical description of the sciatic nerve from the spinal cord to the peripheral branches in the leg. A standardized protocol for carrying out sciatic nerve axotomy is proposed, with guides to assist in the accurate and reliable dissection of the peripheral and central branches of the nerve. Functional, histological, and biochemical criteria for the validation of the injury are described. Thus, this paper provides a review of the principal features of sciatic nerve injury, presents detailed neuroanatomical descriptions of the rat´s inferior limb and spine, compares different modes of injury, offers material for training purposes, and summarizes the immediate and longterm consequences of damage to the sciatic nerve.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32044
Patterson, Sean Ingram; Gonzalez Polo, Virginia; Rasmussen, Jorge A.; Fitt, Marcos R.; Laurito, Sergio Roberto; Savastano, Luis E.; et al.; Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery; Elsevier; Journal of Neuroscience Methods; 227; 1-2014; 166-180
0165-0270
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32044
identifier_str_mv Patterson, Sean Ingram; Gonzalez Polo, Virginia; Rasmussen, Jorge A.; Fitt, Marcos R.; Laurito, Sergio Roberto; Savastano, Luis E.; et al.; Sciatic nerve injury: A simple and subtle model for investigating many aspects of nervous system damage and recovery; Elsevier; Journal of Neuroscience Methods; 227; 1-2014; 166-180
0165-0270
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0165027014000314
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jneumeth.2014.01.020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613284291936256
score 13.070432

Publicaciones similares