One step histological detection and staining of the pten tumor suppressor protein by a single strand dna

Autores
Longinotti, María Gloria; Ybarra, Gabriel Omar; Vighi, Susana; Perandones, Claudia; Montserrat, Javier Marcelo; Yakisich, Juan Sebastian; Grasselli, Mariano; Radrizzani Helguera, Martin
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antibodies are the most used technological tool in histochemistry. However, even with monoclonal antibodies, their standardization is difficult due to variation of biological systems as well as to variability due to the affinity and amplification of the signal arising from secondary peroxidase detection systems. In this article we combined two synthetic molecules to facilitate the standardization of a detection protocol of protein markers in histological sections. The first molecule was an aptamer, a 50-base single-stranded DNA fragment, which recognizes a PTEN tumor suppressor. The second molecule used was also another single stranded 18-base aptamer DNA fragment, which forms a quadruplex structure guanine box. This G-quadruplex recognizes and attaches a molecule of hemin, increasing the catalytic capacity for the hydrogen peroxide. Our results show how the correct structural design of DNA combining an aptamer together with the peroxidase-like DNAzyme allows to detect proteins in histological sections. This tool offers the standardization of the detection of prognostic markers in cancer, in quality and quantity, due to its synthetic nature and its 1:1 antigen:enzyme ratio. This is the first time that reproducible results have been presented in histological sections staining a cancer marker using a single-stranded DNA molecule with dual function.
Fil: Longinotti, María Gloria. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Ybarra, Gabriel Omar. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Vighi, Susana. Universidad de Buenos Aires; Argentina
Fil: Perandones, Claudia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Montserrat, Javier Marcelo. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yakisich, Juan Sebastian. Hampton University; Estados Unidos
Fil: Grasselli, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Radrizzani Helguera, Martin. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
APTAMER
DNAZYME-PEROXIDASE
ENDOMETRIUM
HISTOCHEMISTRY
PTEN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163848
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/163848
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling One step histological detection and staining of the pten tumor suppressor protein by a single strand dnaLonginotti, María GloriaYbarra, Gabriel OmarVighi, SusanaPerandones, ClaudiaMontserrat, Javier MarceloYakisich, Juan SebastianGrasselli, MarianoRadrizzani Helguera, MartinAPTAMERDNAZYME-PEROXIDASEENDOMETRIUMHISTOCHEMISTRYPTENhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Antibodies are the most used technological tool in histochemistry. However, even with monoclonal antibodies, their standardization is difficult due to variation of biological systems as well as to variability due to the affinity and amplification of the signal arising from secondary peroxidase detection systems. In this article we combined two synthetic molecules to facilitate the standardization of a detection protocol of protein markers in histological sections. The first molecule was an aptamer, a 50-base single-stranded DNA fragment, which recognizes a PTEN tumor suppressor. The second molecule used was also another single stranded 18-base aptamer DNA fragment, which forms a quadruplex structure guanine box. This G-quadruplex recognizes and attaches a molecule of hemin, increasing the catalytic capacity for the hydrogen peroxide. Our results show how the correct structural design of DNA combining an aptamer together with the peroxidase-like DNAzyme allows to detect proteins in histological sections. This tool offers the standardization of the detection of prognostic markers in cancer, in quality and quantity, due to its synthetic nature and its 1:1 antigen:enzyme ratio. This is the first time that reproducible results have been presented in histological sections staining a cancer marker using a single-stranded DNA molecule with dual function.Fil: Longinotti, María Gloria. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Ybarra, Gabriel Omar. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Vighi, Susana. Universidad de Buenos Aires; ArgentinaFil: Perandones, Claudia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; ArgentinaFil: Montserrat, Javier Marcelo. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Yakisich, Juan Sebastian. Hampton University; Estados UnidosFil: Grasselli, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Radrizzani Helguera, Martin. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI AG2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163848Longinotti, María Gloria; Ybarra, Gabriel Omar; Vighi, Susana; Perandones, Claudia; Montserrat, Javier Marcelo; et al.; One step histological detection and staining of the pten tumor suppressor protein by a single strand dna; MDPI AG; Diagnostics; 11; 2; 1-2021; 1-132075-4418CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-4418/11/2/171info:eu-repo/semantics/altIdentifier/doi/10.3390/diagnostics11020171info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:25Zoai:ri.conicet.gov.ar:11336/163848instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:26.596CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv One step histological detection and staining of the pten tumor suppressor protein by a single strand dna
title One step histological detection and staining of the pten tumor suppressor protein by a single strand dna
spellingShingle One step histological detection and staining of the pten tumor suppressor protein by a single strand dna
Longinotti, María Gloria
APTAMER
DNAZYME-PEROXIDASE
ENDOMETRIUM
HISTOCHEMISTRY
PTEN
title_short One step histological detection and staining of the pten tumor suppressor protein by a single strand dna
title_full One step histological detection and staining of the pten tumor suppressor protein by a single strand dna
title_fullStr One step histological detection and staining of the pten tumor suppressor protein by a single strand dna
title_full_unstemmed One step histological detection and staining of the pten tumor suppressor protein by a single strand dna
title_sort One step histological detection and staining of the pten tumor suppressor protein by a single strand dna
dc.creator.none.fl_str_mv Longinotti, María Gloria
Ybarra, Gabriel Omar
Vighi, Susana
Perandones, Claudia
Montserrat, Javier Marcelo
Yakisich, Juan Sebastian
Grasselli, Mariano
Radrizzani Helguera, Martin
author Longinotti, María Gloria
author_facet Longinotti, María Gloria
Ybarra, Gabriel Omar
Vighi, Susana
Perandones, Claudia
Montserrat, Javier Marcelo
Yakisich, Juan Sebastian
Grasselli, Mariano
Radrizzani Helguera, Martin
author_role author
author2 Ybarra, Gabriel Omar
Vighi, Susana
Perandones, Claudia
Montserrat, Javier Marcelo
Yakisich, Juan Sebastian
Grasselli, Mariano
Radrizzani Helguera, Martin
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APTAMER
DNAZYME-PEROXIDASE
ENDOMETRIUM
HISTOCHEMISTRY
PTEN
topic APTAMER
DNAZYME-PEROXIDASE
ENDOMETRIUM
HISTOCHEMISTRY
PTEN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Antibodies are the most used technological tool in histochemistry. However, even with monoclonal antibodies, their standardization is difficult due to variation of biological systems as well as to variability due to the affinity and amplification of the signal arising from secondary peroxidase detection systems. In this article we combined two synthetic molecules to facilitate the standardization of a detection protocol of protein markers in histological sections. The first molecule was an aptamer, a 50-base single-stranded DNA fragment, which recognizes a PTEN tumor suppressor. The second molecule used was also another single stranded 18-base aptamer DNA fragment, which forms a quadruplex structure guanine box. This G-quadruplex recognizes and attaches a molecule of hemin, increasing the catalytic capacity for the hydrogen peroxide. Our results show how the correct structural design of DNA combining an aptamer together with the peroxidase-like DNAzyme allows to detect proteins in histological sections. This tool offers the standardization of the detection of prognostic markers in cancer, in quality and quantity, due to its synthetic nature and its 1:1 antigen:enzyme ratio. This is the first time that reproducible results have been presented in histological sections staining a cancer marker using a single-stranded DNA molecule with dual function.
Fil: Longinotti, María Gloria. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Ybarra, Gabriel Omar. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Vighi, Susana. Universidad de Buenos Aires; Argentina
Fil: Perandones, Claudia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Montserrat, Javier Marcelo. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yakisich, Juan Sebastian. Hampton University; Estados Unidos
Fil: Grasselli, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Radrizzani Helguera, Martin. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Antibodies are the most used technological tool in histochemistry. However, even with monoclonal antibodies, their standardization is difficult due to variation of biological systems as well as to variability due to the affinity and amplification of the signal arising from secondary peroxidase detection systems. In this article we combined two synthetic molecules to facilitate the standardization of a detection protocol of protein markers in histological sections. The first molecule was an aptamer, a 50-base single-stranded DNA fragment, which recognizes a PTEN tumor suppressor. The second molecule used was also another single stranded 18-base aptamer DNA fragment, which forms a quadruplex structure guanine box. This G-quadruplex recognizes and attaches a molecule of hemin, increasing the catalytic capacity for the hydrogen peroxide. Our results show how the correct structural design of DNA combining an aptamer together with the peroxidase-like DNAzyme allows to detect proteins in histological sections. This tool offers the standardization of the detection of prognostic markers in cancer, in quality and quantity, due to its synthetic nature and its 1:1 antigen:enzyme ratio. This is the first time that reproducible results have been presented in histological sections staining a cancer marker using a single-stranded DNA molecule with dual function.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163848
Longinotti, María Gloria; Ybarra, Gabriel Omar; Vighi, Susana; Perandones, Claudia; Montserrat, Javier Marcelo; et al.; One step histological detection and staining of the pten tumor suppressor protein by a single strand dna; MDPI AG; Diagnostics; 11; 2; 1-2021; 1-13
2075-4418
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163848
identifier_str_mv Longinotti, María Gloria; Ybarra, Gabriel Omar; Vighi, Susana; Perandones, Claudia; Montserrat, Javier Marcelo; et al.; One step histological detection and staining of the pten tumor suppressor protein by a single strand dna; MDPI AG; Diagnostics; 11; 2; 1-2021; 1-13
2075-4418
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-4418/11/2/171
info:eu-repo/semantics/altIdentifier/doi/10.3390/diagnostics11020171
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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