Proteome variation of the rat liver after static cold storage assayed in an ex vivo model
- Autores
- Knecht, Camila Ayelén; Balaban, Cecilia Lucía; Rodríguez, Joaquín V.; Ceccarelli, Eduardo Augusto; Guibert, Edgardo Elvio; Rosano, German Leandro
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then, the livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Protein identification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry (MALDI‐TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion.
Fil: Knecht, Camila Ayelén. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Rodríguez, Joaquín V.. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; Argentina
Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; Argentina
Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina - Materia
-
COLD ISCHEMIA
LIVER
PROTEOMICS
REPERFUSION INJURY
TWO-DIMENSIONAL GEL ELECTROPHORESIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/100789
Ver los metadatos del registro completo
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Proteome variation of the rat liver after static cold storage assayed in an ex vivo modelKnecht, Camila AyelénBalaban, Cecilia LucíaRodríguez, Joaquín V.Ceccarelli, Eduardo AugustoGuibert, Edgardo ElvioRosano, German LeandroCOLD ISCHEMIALIVERPROTEOMICSREPERFUSION INJURYTWO-DIMENSIONAL GEL ELECTROPHORESIShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then, the livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Protein identification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry (MALDI‐TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion.Fil: Knecht, Camila Ayelén. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Rodríguez, Joaquín V.. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; ArgentinaFil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; ArgentinaFil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaAcademic Press Inc Elsevier Science2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100789Knecht, Camila Ayelén; Balaban, Cecilia Lucía; Rodríguez, Joaquín V.; Ceccarelli, Eduardo Augusto; Guibert, Edgardo Elvio; et al.; Proteome variation of the rat liver after static cold storage assayed in an ex vivo model; Academic Press Inc Elsevier Science; Cryobiology; 85; 12-2018; 47-550011-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0011224018301731info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cryobiol.2018.10.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:08Zoai:ri.conicet.gov.ar:11336/100789instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:08.557CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Proteome variation of the rat liver after static cold storage assayed in an ex vivo model |
title |
Proteome variation of the rat liver after static cold storage assayed in an ex vivo model |
spellingShingle |
Proteome variation of the rat liver after static cold storage assayed in an ex vivo model Knecht, Camila Ayelén COLD ISCHEMIA LIVER PROTEOMICS REPERFUSION INJURY TWO-DIMENSIONAL GEL ELECTROPHORESIS |
title_short |
Proteome variation of the rat liver after static cold storage assayed in an ex vivo model |
title_full |
Proteome variation of the rat liver after static cold storage assayed in an ex vivo model |
title_fullStr |
Proteome variation of the rat liver after static cold storage assayed in an ex vivo model |
title_full_unstemmed |
Proteome variation of the rat liver after static cold storage assayed in an ex vivo model |
title_sort |
Proteome variation of the rat liver after static cold storage assayed in an ex vivo model |
dc.creator.none.fl_str_mv |
Knecht, Camila Ayelén Balaban, Cecilia Lucía Rodríguez, Joaquín V. Ceccarelli, Eduardo Augusto Guibert, Edgardo Elvio Rosano, German Leandro |
author |
Knecht, Camila Ayelén |
author_facet |
Knecht, Camila Ayelén Balaban, Cecilia Lucía Rodríguez, Joaquín V. Ceccarelli, Eduardo Augusto Guibert, Edgardo Elvio Rosano, German Leandro |
author_role |
author |
author2 |
Balaban, Cecilia Lucía Rodríguez, Joaquín V. Ceccarelli, Eduardo Augusto Guibert, Edgardo Elvio Rosano, German Leandro |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
COLD ISCHEMIA LIVER PROTEOMICS REPERFUSION INJURY TWO-DIMENSIONAL GEL ELECTROPHORESIS |
topic |
COLD ISCHEMIA LIVER PROTEOMICS REPERFUSION INJURY TWO-DIMENSIONAL GEL ELECTROPHORESIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then, the livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Protein identification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry (MALDI‐TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion. Fil: Knecht, Camila Ayelén. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Rodríguez, Joaquín V.. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; Argentina Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; Argentina Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina |
description |
Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then, the livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Protein identification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry (MALDI‐TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/100789 Knecht, Camila Ayelén; Balaban, Cecilia Lucía; Rodríguez, Joaquín V.; Ceccarelli, Eduardo Augusto; Guibert, Edgardo Elvio; et al.; Proteome variation of the rat liver after static cold storage assayed in an ex vivo model; Academic Press Inc Elsevier Science; Cryobiology; 85; 12-2018; 47-55 0011-2240 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/100789 |
identifier_str_mv |
Knecht, Camila Ayelén; Balaban, Cecilia Lucía; Rodríguez, Joaquín V.; Ceccarelli, Eduardo Augusto; Guibert, Edgardo Elvio; et al.; Proteome variation of the rat liver after static cold storage assayed in an ex vivo model; Academic Press Inc Elsevier Science; Cryobiology; 85; 12-2018; 47-55 0011-2240 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0011224018301731 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cryobiol.2018.10.003 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613469231382528 |
score |
13.070432 |