Proteome variation of the rat liver after static cold storage assayed in an ex vivo model

Autores
Knecht, Camila Ayelén; Balaban, Cecilia Lucía; Rodríguez, Joaquín V.; Ceccarelli, Eduardo Augusto; Guibert, Edgardo Elvio; Rosano, German Leandro
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then, the livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Protein identification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry (MALDI‐TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion.
Fil: Knecht, Camila Ayelén. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Rodríguez, Joaquín V.. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina
Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina
Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
COLD ISCHEMIA
LIVER
PROTEOMICS
REPERFUSION INJURY
TWO-DIMENSIONAL GEL ELECTROPHORESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100789

id CONICETDig_a6351f5a1758aeca7dac26804a9bb450
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proteome variation of the rat liver after static cold storage assayed in an ex vivo modelKnecht, Camila AyelénBalaban, Cecilia LucíaRodríguez, Joaquín V.Ceccarelli, Eduardo AugustoGuibert, Edgardo ElvioRosano, German LeandroCOLD ISCHEMIALIVERPROTEOMICSREPERFUSION INJURYTWO-DIMENSIONAL GEL ELECTROPHORESIShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then, the livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Protein identification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry (MALDI‐TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion.Fil: Knecht, Camila Ayelén. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Rodríguez, Joaquín V.. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; ArgentinaFil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; ArgentinaFil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaAcademic Press Inc Elsevier Science2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100789Knecht, Camila Ayelén; Balaban, Cecilia Lucía; Rodríguez, Joaquín V.; Ceccarelli, Eduardo Augusto; Guibert, Edgardo Elvio; et al.; Proteome variation of the rat liver after static cold storage assayed in an ex vivo model; Academic Press Inc Elsevier Science; Cryobiology; 85; 12-2018; 47-550011-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0011224018301731info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cryobiol.2018.10.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:08Zoai:ri.conicet.gov.ar:11336/100789instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:08.557CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteome variation of the rat liver after static cold storage assayed in an ex vivo model
title Proteome variation of the rat liver after static cold storage assayed in an ex vivo model
spellingShingle Proteome variation of the rat liver after static cold storage assayed in an ex vivo model
Knecht, Camila Ayelén
COLD ISCHEMIA
LIVER
PROTEOMICS
REPERFUSION INJURY
TWO-DIMENSIONAL GEL ELECTROPHORESIS
title_short Proteome variation of the rat liver after static cold storage assayed in an ex vivo model
title_full Proteome variation of the rat liver after static cold storage assayed in an ex vivo model
title_fullStr Proteome variation of the rat liver after static cold storage assayed in an ex vivo model
title_full_unstemmed Proteome variation of the rat liver after static cold storage assayed in an ex vivo model
title_sort Proteome variation of the rat liver after static cold storage assayed in an ex vivo model
dc.creator.none.fl_str_mv Knecht, Camila Ayelén
Balaban, Cecilia Lucía
Rodríguez, Joaquín V.
Ceccarelli, Eduardo Augusto
Guibert, Edgardo Elvio
Rosano, German Leandro
author Knecht, Camila Ayelén
author_facet Knecht, Camila Ayelén
Balaban, Cecilia Lucía
Rodríguez, Joaquín V.
Ceccarelli, Eduardo Augusto
Guibert, Edgardo Elvio
Rosano, German Leandro
author_role author
author2 Balaban, Cecilia Lucía
Rodríguez, Joaquín V.
Ceccarelli, Eduardo Augusto
Guibert, Edgardo Elvio
Rosano, German Leandro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv COLD ISCHEMIA
LIVER
PROTEOMICS
REPERFUSION INJURY
TWO-DIMENSIONAL GEL ELECTROPHORESIS
topic COLD ISCHEMIA
LIVER
PROTEOMICS
REPERFUSION INJURY
TWO-DIMENSIONAL GEL ELECTROPHORESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then, the livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Protein identification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry (MALDI‐TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion.
Fil: Knecht, Camila Ayelén. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Rodríguez, Joaquín V.. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina
Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina
Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then, the livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Protein identification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry (MALDI‐TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100789
Knecht, Camila Ayelén; Balaban, Cecilia Lucía; Rodríguez, Joaquín V.; Ceccarelli, Eduardo Augusto; Guibert, Edgardo Elvio; et al.; Proteome variation of the rat liver after static cold storage assayed in an ex vivo model; Academic Press Inc Elsevier Science; Cryobiology; 85; 12-2018; 47-55
0011-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100789
identifier_str_mv Knecht, Camila Ayelén; Balaban, Cecilia Lucía; Rodríguez, Joaquín V.; Ceccarelli, Eduardo Augusto; Guibert, Edgardo Elvio; et al.; Proteome variation of the rat liver after static cold storage assayed in an ex vivo model; Academic Press Inc Elsevier Science; Cryobiology; 85; 12-2018; 47-55
0011-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0011224018301731
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cryobiol.2018.10.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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