α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study

Autores
Casciato, Paola; Ambrosi, Nella Gabriela; Caro, Fiorella Yanina; Vazquez, Mónica; Müllen, Eduardo; Gadano, Adrián Carlos; de Santibanes, Eduardo; de Santibañes, Martin; Zandomeni, Marcos; Chahdi, Magali; Lazarte, Julio C.; Biagiola, David A.; Iaquinandi, Juan Cruz; Santofimia-Castaño, Patricia; Iovanna, Juan Lucio; Incardona, Claudio; Chuluyan, Hector Eduardo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A double-blind randomized controlled trial was performed to compare the safety and efficacy of α-lipoic acid (ALA) in liver transplantation (LT). The grafts were randomized to receive ALA or placebo before the cold ischemia time. Furthermore, patients transplanted with the ALA-perfused graft received 600 mg of intravenous ALA, while patients with the nonperfused graft received the placebo just before graft reperfusion. Hepatic biopsy was performed 2 h postreperfusion. Blood samples were collected before, during and 1 and 2 days after reperfusion. Quantitative polymerase chain reaction (qPCR) analysis was performed on biopsies to assess genes involved in the response to hypoxia, apoptosis, cell growth, survival and proliferation, cytokine production and tissue damage protection. Nine of 40 patients developed postreperfusion syndrome (PRS), but seven of them belonged to the control group. There was a decrease in PHD2 and an increase in alpha subunit of hypoxia-inducible factor-1 (HIF-1α) and baculoviral IAP repeat containing 2 (Birc2) transcript levels in the biopsies from the ALA-treated versus the control group of patients. Additionally, plasma levels of alarmins were lower in ALA-treated patients than control patients, which suggests that ALA-treated grafts are less inflammatory than untreated grafts. These results showed that ALA is safe for use in LT, induces gene changes that protect against hypoxia and oxidative stress and reduces the appearance of PRS.
Fil: Casciato, Paola. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Caro, Fiorella Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Vazquez, Mónica. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Müllen, Eduardo. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Gadano, Adrián Carlos. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: de Santibanes, Eduardo. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: de Santibañes, Martin. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Zandomeni, Marcos. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Chahdi, Magali. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Lazarte, Julio C.. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Biagiola, David A.. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Iaquinandi, Juan Cruz. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Santofimia-Castaño, Patricia. Centre de Recherche En Cancerologie de Marseille; Francia
Fil: Iovanna, Juan Lucio. Centre de Recherche En Cancerologie de Marseille; Francia
Fil: Incardona, Claudio. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina. Fundación Gador; Argentina
Fil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Materia
ALPHA LIPOIC ACID
ISCHEMIA REPERFUSION INJURY
LIVER TRANSPLANTATION
POSTREPERFUSION SYNDROME
SLPI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/89751
Acceder
id CONICETDig_85b9c2bad2bd83892c5590aac7f11119
oai_identifier_str oai:ri.conicet.gov.ar:11336/89751
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot studyCasciato, PaolaAmbrosi, Nella GabrielaCaro, Fiorella YaninaVazquez, MónicaMüllen, EduardoGadano, Adrián Carlosde Santibanes, Eduardode Santibañes, MartinZandomeni, MarcosChahdi, MagaliLazarte, Julio C.Biagiola, David A.Iaquinandi, Juan CruzSantofimia-Castaño, PatriciaIovanna, Juan LucioIncardona, ClaudioChuluyan, Hector EduardoALPHA LIPOIC ACIDISCHEMIA REPERFUSION INJURYLIVER TRANSPLANTATIONPOSTREPERFUSION SYNDROMESLPIhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3A double-blind randomized controlled trial was performed to compare the safety and efficacy of α-lipoic acid (ALA) in liver transplantation (LT). The grafts were randomized to receive ALA or placebo before the cold ischemia time. Furthermore, patients transplanted with the ALA-perfused graft received 600 mg of intravenous ALA, while patients with the nonperfused graft received the placebo just before graft reperfusion. Hepatic biopsy was performed 2 h postreperfusion. Blood samples were collected before, during and 1 and 2 days after reperfusion. Quantitative polymerase chain reaction (qPCR) analysis was performed on biopsies to assess genes involved in the response to hypoxia, apoptosis, cell growth, survival and proliferation, cytokine production and tissue damage protection. Nine of 40 patients developed postreperfusion syndrome (PRS), but seven of them belonged to the control group. There was a decrease in PHD2 and an increase in alpha subunit of hypoxia-inducible factor-1 (HIF-1α) and baculoviral IAP repeat containing 2 (Birc2) transcript levels in the biopsies from the ALA-treated versus the control group of patients. Additionally, plasma levels of alarmins were lower in ALA-treated patients than control patients, which suggests that ALA-treated grafts are less inflammatory than untreated grafts. These results showed that ALA is safe for use in LT, induces gene changes that protect against hypoxia and oxidative stress and reduces the appearance of PRS.Fil: Casciato, Paola. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Caro, Fiorella Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Vazquez, Mónica. Instituto Universitario del Hospital Italiano de Buenos Aires; ArgentinaFil: Müllen, Eduardo. Instituto Universitario del Hospital Italiano de Buenos Aires; ArgentinaFil: Gadano, Adrián Carlos. Instituto Universitario del Hospital Italiano de Buenos Aires; ArgentinaFil: de Santibanes, Eduardo. Instituto Universitario del Hospital Italiano de Buenos Aires; ArgentinaFil: de Santibañes, Martin. Instituto Universitario del Hospital Italiano de Buenos Aires; ArgentinaFil: Zandomeni, Marcos. Instituto Universitario del Hospital Italiano de Buenos Aires; ArgentinaFil: Chahdi, Magali. Instituto Universitario del Hospital Italiano de Buenos Aires; ArgentinaFil: Lazarte, Julio C.. Instituto Universitario del Hospital Italiano de Buenos Aires; ArgentinaFil: Biagiola, David A.. Instituto Universitario del Hospital Italiano de Buenos Aires; ArgentinaFil: Iaquinandi, Juan Cruz. Instituto Universitario del Hospital Italiano de Buenos Aires; ArgentinaFil: Santofimia-Castaño, Patricia. Centre de Recherche En Cancerologie de Marseille; FranciaFil: Iovanna, Juan Lucio. Centre de Recherche En Cancerologie de Marseille; FranciaFil: Incardona, Claudio. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina. Fundación Gador; ArgentinaFil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaWiley Blackwell Publishing, Inc2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89751Casciato, Paola; Ambrosi, Nella Gabriela; Caro, Fiorella Yanina; Vazquez, Mónica; Müllen, Eduardo; et al.; α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study; Wiley Blackwell Publishing, Inc; Transplant International; 31; 12; 12-2018; 1357-13680934-0874CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/tri.13314info:eu-repo/semantics/altIdentifier/doi/10.1111/tri.13314info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:18:19Zoai:ri.conicet.gov.ar:11336/89751instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:18:19.416CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study
title α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study
spellingShingle α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study
Casciato, Paola
ALPHA LIPOIC ACID
ISCHEMIA REPERFUSION INJURY
LIVER TRANSPLANTATION
POSTREPERFUSION SYNDROME
SLPI
title_short α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study
title_full α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study
title_fullStr α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study
title_full_unstemmed α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study
title_sort α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study
dc.creator.none.fl_str_mv Casciato, Paola
Ambrosi, Nella Gabriela
Caro, Fiorella Yanina
Vazquez, Mónica
Müllen, Eduardo
Gadano, Adrián Carlos
de Santibanes, Eduardo
de Santibañes, Martin
Zandomeni, Marcos
Chahdi, Magali
Lazarte, Julio C.
Biagiola, David A.
Iaquinandi, Juan Cruz
Santofimia-Castaño, Patricia
Iovanna, Juan Lucio
Incardona, Claudio
Chuluyan, Hector Eduardo
author Casciato, Paola
author_facet Casciato, Paola
Ambrosi, Nella Gabriela
Caro, Fiorella Yanina
Vazquez, Mónica
Müllen, Eduardo
Gadano, Adrián Carlos
de Santibanes, Eduardo
de Santibañes, Martin
Zandomeni, Marcos
Chahdi, Magali
Lazarte, Julio C.
Biagiola, David A.
Iaquinandi, Juan Cruz
Santofimia-Castaño, Patricia
Iovanna, Juan Lucio
Incardona, Claudio
Chuluyan, Hector Eduardo
author_role author
author2 Ambrosi, Nella Gabriela
Caro, Fiorella Yanina
Vazquez, Mónica
Müllen, Eduardo
Gadano, Adrián Carlos
de Santibanes, Eduardo
de Santibañes, Martin
Zandomeni, Marcos
Chahdi, Magali
Lazarte, Julio C.
Biagiola, David A.
Iaquinandi, Juan Cruz
Santofimia-Castaño, Patricia
Iovanna, Juan Lucio
Incardona, Claudio
Chuluyan, Hector Eduardo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ALPHA LIPOIC ACID
ISCHEMIA REPERFUSION INJURY
LIVER TRANSPLANTATION
POSTREPERFUSION SYNDROME
SLPI
topic ALPHA LIPOIC ACID
ISCHEMIA REPERFUSION INJURY
LIVER TRANSPLANTATION
POSTREPERFUSION SYNDROME
SLPI
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A double-blind randomized controlled trial was performed to compare the safety and efficacy of α-lipoic acid (ALA) in liver transplantation (LT). The grafts were randomized to receive ALA or placebo before the cold ischemia time. Furthermore, patients transplanted with the ALA-perfused graft received 600 mg of intravenous ALA, while patients with the nonperfused graft received the placebo just before graft reperfusion. Hepatic biopsy was performed 2 h postreperfusion. Blood samples were collected before, during and 1 and 2 days after reperfusion. Quantitative polymerase chain reaction (qPCR) analysis was performed on biopsies to assess genes involved in the response to hypoxia, apoptosis, cell growth, survival and proliferation, cytokine production and tissue damage protection. Nine of 40 patients developed postreperfusion syndrome (PRS), but seven of them belonged to the control group. There was a decrease in PHD2 and an increase in alpha subunit of hypoxia-inducible factor-1 (HIF-1α) and baculoviral IAP repeat containing 2 (Birc2) transcript levels in the biopsies from the ALA-treated versus the control group of patients. Additionally, plasma levels of alarmins were lower in ALA-treated patients than control patients, which suggests that ALA-treated grafts are less inflammatory than untreated grafts. These results showed that ALA is safe for use in LT, induces gene changes that protect against hypoxia and oxidative stress and reduces the appearance of PRS.
Fil: Casciato, Paola. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Caro, Fiorella Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Vazquez, Mónica. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Müllen, Eduardo. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Gadano, Adrián Carlos. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: de Santibanes, Eduardo. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: de Santibañes, Martin. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Zandomeni, Marcos. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Chahdi, Magali. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Lazarte, Julio C.. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Biagiola, David A.. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Iaquinandi, Juan Cruz. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina
Fil: Santofimia-Castaño, Patricia. Centre de Recherche En Cancerologie de Marseille; Francia
Fil: Iovanna, Juan Lucio. Centre de Recherche En Cancerologie de Marseille; Francia
Fil: Incardona, Claudio. Instituto Universitario del Hospital Italiano de Buenos Aires; Argentina. Fundación Gador; Argentina
Fil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
description A double-blind randomized controlled trial was performed to compare the safety and efficacy of α-lipoic acid (ALA) in liver transplantation (LT). The grafts were randomized to receive ALA or placebo before the cold ischemia time. Furthermore, patients transplanted with the ALA-perfused graft received 600 mg of intravenous ALA, while patients with the nonperfused graft received the placebo just before graft reperfusion. Hepatic biopsy was performed 2 h postreperfusion. Blood samples were collected before, during and 1 and 2 days after reperfusion. Quantitative polymerase chain reaction (qPCR) analysis was performed on biopsies to assess genes involved in the response to hypoxia, apoptosis, cell growth, survival and proliferation, cytokine production and tissue damage protection. Nine of 40 patients developed postreperfusion syndrome (PRS), but seven of them belonged to the control group. There was a decrease in PHD2 and an increase in alpha subunit of hypoxia-inducible factor-1 (HIF-1α) and baculoviral IAP repeat containing 2 (Birc2) transcript levels in the biopsies from the ALA-treated versus the control group of patients. Additionally, plasma levels of alarmins were lower in ALA-treated patients than control patients, which suggests that ALA-treated grafts are less inflammatory than untreated grafts. These results showed that ALA is safe for use in LT, induces gene changes that protect against hypoxia and oxidative stress and reduces the appearance of PRS.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/89751
Casciato, Paola; Ambrosi, Nella Gabriela; Caro, Fiorella Yanina; Vazquez, Mónica; Müllen, Eduardo; et al.; α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study; Wiley Blackwell Publishing, Inc; Transplant International; 31; 12; 12-2018; 1357-1368
0934-0874
CONICET Digital
CONICET
url http://hdl.handle.net/11336/89751
identifier_str_mv Casciato, Paola; Ambrosi, Nella Gabriela; Caro, Fiorella Yanina; Vazquez, Mónica; Müllen, Eduardo; et al.; α-lipoic acid reduces postreperfusion syndrome in human liver transplantation - a pilot study; Wiley Blackwell Publishing, Inc; Transplant International; 31; 12; 12-2018; 1357-1368
0934-0874
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/tri.13314
info:eu-repo/semantics/altIdentifier/doi/10.1111/tri.13314
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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