Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?

Autores
Montaner, Alejandro Daniel; Gonzalez, Olga Mabel; Paz, Dante Agustin; Affanni, Jorge Mario; Somoza, Gustavo Manuel
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In reptiles as in other vertebrates, multiple forms of gonadotropin-releasing hormone (GnRH) within a single brain have been identified. In this group the following GnRH molecular variants have been characterized either by direct or indirect methods: chicken GnRH I (cGnRH-I), chicken GnRH II (cGnRH-II), salmon GnRH (sGnRH) and several unidentified GnRH-like forms. In the present study GnRH variants were investigated in brain extracts of the lizard Tupinambis teguixin (= T. merinae) by combining high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassays (RIA). Two peaks showing GnRH immunoreactivity with the elution position of synthetic mammalian GnRH (mGnRH) and cGnRH-II were detected. Both peaks were further analyzed with different radioimmunoassay systems specific for mGnRH, cGnRH-I, and cGnRH-II. Pooled fractions corresponding to the first eluting peak showed no cross-reactivity when analyzed with a cGnRH-I specific assay and logit-log displacement curves were not significantly different from those of synthetic mGnRH with homologous RIA systems. The second peak showed immunological characteristics of cGnRH-II when analyzed with a specific antiserum. The first ir-GnRH peak was selected for further RP-HPLC purification showing similar chromatographic behavior as mGnRH synthetic standard. We demonstrated the absence of cGnRH-I in this lizard using well-characterized antisera. (C) 2000 Academic Press.
Fil: Montaner, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gonzalez, Olga Mabel. Universidad de Buenos Aires. Facultad de Agronomia. Pabellon de Zootécnica; Argentina
Fil: Paz, Dante Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Embriología Animal; Argentina
Fil: Affanni, Jorge Mario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina
Materia
Brain
Gonadotropin-Releasing Hormone
Reptiles
Ria
Rp-Hplc
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53396
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53396
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?Montaner, Alejandro DanielGonzalez, Olga MabelPaz, Dante AgustinAffanni, Jorge MarioSomoza, Gustavo ManuelBrainGonadotropin-Releasing HormoneReptilesRiaRp-Hplchttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In reptiles as in other vertebrates, multiple forms of gonadotropin-releasing hormone (GnRH) within a single brain have been identified. In this group the following GnRH molecular variants have been characterized either by direct or indirect methods: chicken GnRH I (cGnRH-I), chicken GnRH II (cGnRH-II), salmon GnRH (sGnRH) and several unidentified GnRH-like forms. In the present study GnRH variants were investigated in brain extracts of the lizard Tupinambis teguixin (= T. merinae) by combining high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassays (RIA). Two peaks showing GnRH immunoreactivity with the elution position of synthetic mammalian GnRH (mGnRH) and cGnRH-II were detected. Both peaks were further analyzed with different radioimmunoassay systems specific for mGnRH, cGnRH-I, and cGnRH-II. Pooled fractions corresponding to the first eluting peak showed no cross-reactivity when analyzed with a cGnRH-I specific assay and logit-log displacement curves were not significantly different from those of synthetic mGnRH with homologous RIA systems. The second peak showed immunological characteristics of cGnRH-II when analyzed with a specific antiserum. The first ir-GnRH peak was selected for further RP-HPLC purification showing similar chromatographic behavior as mGnRH synthetic standard. We demonstrated the absence of cGnRH-I in this lizard using well-characterized antisera. (C) 2000 Academic Press.Fil: Montaner, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Gonzalez, Olga Mabel. Universidad de Buenos Aires. Facultad de Agronomia. Pabellon de Zootécnica; ArgentinaFil: Paz, Dante Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Embriología Animal; ArgentinaFil: Affanni, Jorge Mario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; ArgentinaAcademic Press Inc Elsevier Science2000-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/53396Montaner, Alejandro Daniel; Gonzalez, Olga Mabel; Paz, Dante Agustin; Affanni, Jorge Mario; Somoza, Gustavo Manuel; Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 119; 2; 3-2000; 121-1310016-6480CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1006/gcen.2000.7488info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0016648000974884info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:38Zoai:ri.conicet.gov.ar:11336/53396instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:38.878CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
title Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
spellingShingle Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
Montaner, Alejandro Daniel
Brain
Gonadotropin-Releasing Hormone
Reptiles
Ria
Rp-Hplc
title_short Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
title_full Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
title_fullStr Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
title_full_unstemmed Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
title_sort Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
dc.creator.none.fl_str_mv Montaner, Alejandro Daniel
Gonzalez, Olga Mabel
Paz, Dante Agustin
Affanni, Jorge Mario
Somoza, Gustavo Manuel
author Montaner, Alejandro Daniel
author_facet Montaner, Alejandro Daniel
Gonzalez, Olga Mabel
Paz, Dante Agustin
Affanni, Jorge Mario
Somoza, Gustavo Manuel
author_role author
author2 Gonzalez, Olga Mabel
Paz, Dante Agustin
Affanni, Jorge Mario
Somoza, Gustavo Manuel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Brain
Gonadotropin-Releasing Hormone
Reptiles
Ria
Rp-Hplc
topic Brain
Gonadotropin-Releasing Hormone
Reptiles
Ria
Rp-Hplc
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In reptiles as in other vertebrates, multiple forms of gonadotropin-releasing hormone (GnRH) within a single brain have been identified. In this group the following GnRH molecular variants have been characterized either by direct or indirect methods: chicken GnRH I (cGnRH-I), chicken GnRH II (cGnRH-II), salmon GnRH (sGnRH) and several unidentified GnRH-like forms. In the present study GnRH variants were investigated in brain extracts of the lizard Tupinambis teguixin (= T. merinae) by combining high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassays (RIA). Two peaks showing GnRH immunoreactivity with the elution position of synthetic mammalian GnRH (mGnRH) and cGnRH-II were detected. Both peaks were further analyzed with different radioimmunoassay systems specific for mGnRH, cGnRH-I, and cGnRH-II. Pooled fractions corresponding to the first eluting peak showed no cross-reactivity when analyzed with a cGnRH-I specific assay and logit-log displacement curves were not significantly different from those of synthetic mGnRH with homologous RIA systems. The second peak showed immunological characteristics of cGnRH-II when analyzed with a specific antiserum. The first ir-GnRH peak was selected for further RP-HPLC purification showing similar chromatographic behavior as mGnRH synthetic standard. We demonstrated the absence of cGnRH-I in this lizard using well-characterized antisera. (C) 2000 Academic Press.
Fil: Montaner, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gonzalez, Olga Mabel. Universidad de Buenos Aires. Facultad de Agronomia. Pabellon de Zootécnica; Argentina
Fil: Paz, Dante Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Embriología Animal; Argentina
Fil: Affanni, Jorge Mario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina
description In reptiles as in other vertebrates, multiple forms of gonadotropin-releasing hormone (GnRH) within a single brain have been identified. In this group the following GnRH molecular variants have been characterized either by direct or indirect methods: chicken GnRH I (cGnRH-I), chicken GnRH II (cGnRH-II), salmon GnRH (sGnRH) and several unidentified GnRH-like forms. In the present study GnRH variants were investigated in brain extracts of the lizard Tupinambis teguixin (= T. merinae) by combining high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassays (RIA). Two peaks showing GnRH immunoreactivity with the elution position of synthetic mammalian GnRH (mGnRH) and cGnRH-II were detected. Both peaks were further analyzed with different radioimmunoassay systems specific for mGnRH, cGnRH-I, and cGnRH-II. Pooled fractions corresponding to the first eluting peak showed no cross-reactivity when analyzed with a cGnRH-I specific assay and logit-log displacement curves were not significantly different from those of synthetic mGnRH with homologous RIA systems. The second peak showed immunological characteristics of cGnRH-II when analyzed with a specific antiserum. The first ir-GnRH peak was selected for further RP-HPLC purification showing similar chromatographic behavior as mGnRH synthetic standard. We demonstrated the absence of cGnRH-I in this lizard using well-characterized antisera. (C) 2000 Academic Press.
publishDate 2000
dc.date.none.fl_str_mv 2000-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53396
Montaner, Alejandro Daniel; Gonzalez, Olga Mabel; Paz, Dante Agustin; Affanni, Jorge Mario; Somoza, Gustavo Manuel; Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 119; 2; 3-2000; 121-131
0016-6480
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53396
identifier_str_mv Montaner, Alejandro Daniel; Gonzalez, Olga Mabel; Paz, Dante Agustin; Affanni, Jorge Mario; Somoza, Gustavo Manuel; Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 119; 2; 3-2000; 121-131
0016-6480
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1006/gcen.2000.7488
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0016648000974884
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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