Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents

Autores
Montaner, Alejandro Daniel; Mongiat, Lucas Alberto; Lux, Victoria Adela R.; Warby, Carol; Chewpoy, Brad; Bianchi, Maria Silvia; Libertun, Carlos; Rivier, Jean E.; Sherwood, Nancy M.; Somoza, Gustavo Manuel
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gonadotropin-releasing hormone (GnRH) is a decapeptide widely known for its role in regulating vertebrate reproduction by serving as a signal from the hypothalamus to pituitary gonadotropes. The first form of GnRH to be identified was isolated from mammals (mGnRH) and the same form has been reported for all mammals studied, which includes marsupials and placental mammals. Later, another variant, chicken GnRH-II (cGnRH-II) was shown to be expressed together with mGnRH in the brains of all jawed vertebrates, including mammals such as rats, monkeys and humans. Our objective was to characterize a third form of GnRH that was isolated previously as mRNA from guinea pigs (gpGnRH), but has not been reported for any other mammal to date. Furthermore, the gonadotropic activity of gpGnRH has not been fully characterized. Our results, using chromatographical and immunological methods, show for the first time that gpGnRH is expressed together with mGnRH in some rodents (wild guinea pig and capybara), but not in others (mouse and hamster). Also, the gonadotropic activity of gpGnRH and mGnRH was tested in two different rat cell culture systems. Although there have been reports that the salmon(s) form of GnRH is present in mammals, we did not detect sGnRH in capybara, wild guinea pigs, hamsters, rats or mice. Taken together with previous reports, the present results support the idea that the expression of multiple GnRH variants in a single species is a common pattern in most vertebrate groups
Fil: Montaner, Alejandro Daniel. Fundación Pablo Cassara; Argentina
Fil: Mongiat, Lucas Alberto. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lux, Victoria Adela R.. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Warby, Carol. University Of Victoria; Canadá
Fil: Chewpoy, Brad. University Of Victoria; Canadá
Fil: Bianchi, Maria Silvia. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Libertun, Carlos. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Rivier, Jean E.. Salk Institute for Biological Studies; Estados Unidos
Fil: Sherwood, Nancy M.. University Of Victoria; Canadá
Fil: Somoza, Gustavo Manuel. Fundación Pablo Cassara; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Materia
Gonadotropin-Releasing Hormone
Gonadotropin-Releasing Hormone Analogues
Gonadotropins
Reproduction
Comparative Neuroendocrinology
Guinea Pig
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29584
Acceder
id CONICETDig_4141d0b0b7fce8174aef65641f56eddf
oai_identifier_str oai:ri.conicet.gov.ar:11336/29584
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in RodentsMontaner, Alejandro DanielMongiat, Lucas AlbertoLux, Victoria Adela R.Warby, CarolChewpoy, BradBianchi, Maria SilviaLibertun, CarlosRivier, Jean E.Sherwood, Nancy M.Somoza, Gustavo ManuelGonadotropin-Releasing HormoneGonadotropin-Releasing Hormone AnaloguesGonadotropinsReproductionComparative NeuroendocrinologyGuinea PigGonadotropin-releasing hormone (GnRH) is a decapeptide widely known for its role in regulating vertebrate reproduction by serving as a signal from the hypothalamus to pituitary gonadotropes. The first form of GnRH to be identified was isolated from mammals (mGnRH) and the same form has been reported for all mammals studied, which includes marsupials and placental mammals. Later, another variant, chicken GnRH-II (cGnRH-II) was shown to be expressed together with mGnRH in the brains of all jawed vertebrates, including mammals such as rats, monkeys and humans. Our objective was to characterize a third form of GnRH that was isolated previously as mRNA from guinea pigs (gpGnRH), but has not been reported for any other mammal to date. Furthermore, the gonadotropic activity of gpGnRH has not been fully characterized. Our results, using chromatographical and immunological methods, show for the first time that gpGnRH is expressed together with mGnRH in some rodents (wild guinea pig and capybara), but not in others (mouse and hamster). Also, the gonadotropic activity of gpGnRH and mGnRH was tested in two different rat cell culture systems. Although there have been reports that the salmon(s) form of GnRH is present in mammals, we did not detect sGnRH in capybara, wild guinea pigs, hamsters, rats or mice. Taken together with previous reports, the present results support the idea that the expression of multiple GnRH variants in a single species is a common pattern in most vertebrate groupsFil: Montaner, Alejandro Daniel. Fundación Pablo Cassara; ArgentinaFil: Mongiat, Lucas Alberto. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lux, Victoria Adela R.. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Warby, Carol. University Of Victoria; CanadáFil: Chewpoy, Brad. University Of Victoria; CanadáFil: Bianchi, Maria Silvia. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Libertun, Carlos. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Rivier, Jean E.. Salk Institute for Biological Studies; Estados UnidosFil: Sherwood, Nancy M.. University Of Victoria; CanadáFil: Somoza, Gustavo Manuel. Fundación Pablo Cassara; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaKarger2002-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29584Montaner, Alejandro Daniel; Mongiat, Lucas Alberto; Lux, Victoria Adela R.; Warby, Carol; Chewpoy, Brad; et al.; Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents; Karger; Neuroendocrinology; 75; 5; 12-2002; 326-3380028-3835CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/57342info:eu-repo/semantics/altIdentifier/doi/10.1159/000057342info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:15Zoai:ri.conicet.gov.ar:11336/29584instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:15.867CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents
title Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents
spellingShingle Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents
Montaner, Alejandro Daniel
Gonadotropin-Releasing Hormone
Gonadotropin-Releasing Hormone Analogues
Gonadotropins
Reproduction
Comparative Neuroendocrinology
Guinea Pig
title_short Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents
title_full Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents
title_fullStr Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents
title_full_unstemmed Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents
title_sort Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents
dc.creator.none.fl_str_mv Montaner, Alejandro Daniel
Mongiat, Lucas Alberto
Lux, Victoria Adela R.
Warby, Carol
Chewpoy, Brad
Bianchi, Maria Silvia
Libertun, Carlos
Rivier, Jean E.
Sherwood, Nancy M.
Somoza, Gustavo Manuel
author Montaner, Alejandro Daniel
author_facet Montaner, Alejandro Daniel
Mongiat, Lucas Alberto
Lux, Victoria Adela R.
Warby, Carol
Chewpoy, Brad
Bianchi, Maria Silvia
Libertun, Carlos
Rivier, Jean E.
Sherwood, Nancy M.
Somoza, Gustavo Manuel
author_role author
author2 Mongiat, Lucas Alberto
Lux, Victoria Adela R.
Warby, Carol
Chewpoy, Brad
Bianchi, Maria Silvia
Libertun, Carlos
Rivier, Jean E.
Sherwood, Nancy M.
Somoza, Gustavo Manuel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gonadotropin-Releasing Hormone
Gonadotropin-Releasing Hormone Analogues
Gonadotropins
Reproduction
Comparative Neuroendocrinology
Guinea Pig
topic Gonadotropin-Releasing Hormone
Gonadotropin-Releasing Hormone Analogues
Gonadotropins
Reproduction
Comparative Neuroendocrinology
Guinea Pig
dc.description.none.fl_txt_mv Gonadotropin-releasing hormone (GnRH) is a decapeptide widely known for its role in regulating vertebrate reproduction by serving as a signal from the hypothalamus to pituitary gonadotropes. The first form of GnRH to be identified was isolated from mammals (mGnRH) and the same form has been reported for all mammals studied, which includes marsupials and placental mammals. Later, another variant, chicken GnRH-II (cGnRH-II) was shown to be expressed together with mGnRH in the brains of all jawed vertebrates, including mammals such as rats, monkeys and humans. Our objective was to characterize a third form of GnRH that was isolated previously as mRNA from guinea pigs (gpGnRH), but has not been reported for any other mammal to date. Furthermore, the gonadotropic activity of gpGnRH has not been fully characterized. Our results, using chromatographical and immunological methods, show for the first time that gpGnRH is expressed together with mGnRH in some rodents (wild guinea pig and capybara), but not in others (mouse and hamster). Also, the gonadotropic activity of gpGnRH and mGnRH was tested in two different rat cell culture systems. Although there have been reports that the salmon(s) form of GnRH is present in mammals, we did not detect sGnRH in capybara, wild guinea pigs, hamsters, rats or mice. Taken together with previous reports, the present results support the idea that the expression of multiple GnRH variants in a single species is a common pattern in most vertebrate groups
Fil: Montaner, Alejandro Daniel. Fundación Pablo Cassara; Argentina
Fil: Mongiat, Lucas Alberto. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lux, Victoria Adela R.. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Warby, Carol. University Of Victoria; Canadá
Fil: Chewpoy, Brad. University Of Victoria; Canadá
Fil: Bianchi, Maria Silvia. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Libertun, Carlos. Fundación de Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Rivier, Jean E.. Salk Institute for Biological Studies; Estados Unidos
Fil: Sherwood, Nancy M.. University Of Victoria; Canadá
Fil: Somoza, Gustavo Manuel. Fundación Pablo Cassara; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
description Gonadotropin-releasing hormone (GnRH) is a decapeptide widely known for its role in regulating vertebrate reproduction by serving as a signal from the hypothalamus to pituitary gonadotropes. The first form of GnRH to be identified was isolated from mammals (mGnRH) and the same form has been reported for all mammals studied, which includes marsupials and placental mammals. Later, another variant, chicken GnRH-II (cGnRH-II) was shown to be expressed together with mGnRH in the brains of all jawed vertebrates, including mammals such as rats, monkeys and humans. Our objective was to characterize a third form of GnRH that was isolated previously as mRNA from guinea pigs (gpGnRH), but has not been reported for any other mammal to date. Furthermore, the gonadotropic activity of gpGnRH has not been fully characterized. Our results, using chromatographical and immunological methods, show for the first time that gpGnRH is expressed together with mGnRH in some rodents (wild guinea pig and capybara), but not in others (mouse and hamster). Also, the gonadotropic activity of gpGnRH and mGnRH was tested in two different rat cell culture systems. Although there have been reports that the salmon(s) form of GnRH is present in mammals, we did not detect sGnRH in capybara, wild guinea pigs, hamsters, rats or mice. Taken together with previous reports, the present results support the idea that the expression of multiple GnRH variants in a single species is a common pattern in most vertebrate groups
publishDate 2002
dc.date.none.fl_str_mv 2002-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29584
Montaner, Alejandro Daniel; Mongiat, Lucas Alberto; Lux, Victoria Adela R.; Warby, Carol; Chewpoy, Brad; et al.; Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents; Karger; Neuroendocrinology; 75; 5; 12-2002; 326-338
0028-3835
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29584
identifier_str_mv Montaner, Alejandro Daniel; Mongiat, Lucas Alberto; Lux, Victoria Adela R.; Warby, Carol; Chewpoy, Brad; et al.; Guinea Pig Gonadotropin-Releasing Hormone: Expression Pattern, Characterization and Biological Activity in Rodents; Karger; Neuroendocrinology; 75; 5; 12-2002; 326-338
0028-3835
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/57342
info:eu-repo/semantics/altIdentifier/doi/10.1159/000057342
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269950147821568
score 13.13397

Publicaciones similares