Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives

Autores
Gaillard, Anne Laure; Tay, Boon Hui; Pérez Sirkin, Daniela Irina; Lafont, Anne Gaëlle; De Flori, Céline; Vissio, Paula Gabriela; Mazan, Sylvie; Dufour, Sylvie; Venkatesh, Byrappa; Tostivint, Hervé
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The neuropeptide gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Vertebrates possess multiple GnRH forms that are classified into three main groups, namely GnRH1, GnRH2, and GnRH3. In order to gain more insights into the GnRH gene family in vertebrates, we sought to identify which paralogs of this family are present in cartilaginous fish. For this purpose, we searched the genomes and/or transcriptomes of three representative species of this group, the small-spotted catshark, Scyliorhinus canicula, the whale shark, Rhincodon typus and the elephant shark Callorhinchus milii. In each species, we report the identification of three GnRH genes. In catshark and whale shark, phylogenetic and synteny analysis showed that these three genes correspond to GnRH1, GnRH2, and GnRH3. In both species, GnRH1 was found to encode a novel form of GnRH whose primary structure was determined as follows: QHWSFDLRPG. In elephant shark, the three genes correspond to GnRH1a and GnRH1b, two copies of the GnRH1 gene, plus GnRH2. 3D structure prediction of the chondrichthyan GnRH-associated peptides (GAPs) revealed that catshark GAP1, GAP2, and elephant shark GAP2 peptides exhibit a helix-loop-helix (HLH) structure. This structure observed for many osteichthyan GAP1 and GAP2, may convey GAP biological activity. This HLH structure could not be observed for elephant shark GAP1a and GAP1b. As for all other GAP3 described so far, no typical 3D HLH structure was observed for catshark nor whale shark GAP3. RT-PCR analysis revealed that GnRH1, GnRH2, and GnRH3 genes are differentially expressed in the catshark brain. GnRH1 mRNA appeared predominant in the diencephalon while GnRH2 and GnRH3 mRNAs seemed to be most abundant in the mesencephalon and telencephalon, respectively. Taken together, our results show that the GnRH gene repertoire of the vertebrate ancestor was entirely conserved in the chondrichthyan lineage but that the GnRH3 gene was probably lost in holocephali. They also suggest that the three GnRH neuronal systems previously described in the brain of bony vertebrates are also present in cartilaginous fish.
Fil: Gaillard, Anne Laure. Centre National de la Recherche Scientifique; Francia. Muséum National d'Histoire Naturelle; Francia
Fil: Tay, Boon Hui. A∗STAR. Institute of Molecular and Cell Biology; Singapur
Fil: Pérez Sirkin, Daniela Irina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Lafont, Anne Gaëlle. Muséum National d'Histoire Naturelle; Francia. Centre National de la Recherche Scientifique; Francia
Fil: De Flori, Céline. Centre National de la Recherche Scientifique; Francia. Muséum National d'Histoire Naturelle; Francia
Fil: Vissio, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Mazan, Sylvie. Observatoire Océanlogique de Banyuls, Sorbonne Universi; Francia
Fil: Dufour, Sylvie. Muséum National d'Histoire Naturelle; Francia. Centre National de la Recherche Scientifique; Francia. Universite de la Sorbona Nouvelle; Francia
Fil: Venkatesh, Byrappa. A∗STAR. Institute of Molecular and Cell Biology; Singapur
Fil: Tostivint, Hervé. Muséum National d'Histoire Naturelle; Francia. Centre National de la Recherche Scientifique; Francia
Materia
CARTILAGINOUS FISH
ELASMOBRANCHII
EVOLUTION
GONADOTROPIN-RELEASING HORMONE
HOLOCEPHALI
MULTIGENIC FAMILY
NEUROPEPTIDES
VERTEBRATES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96226
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96226
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary PerspectivesGaillard, Anne LaureTay, Boon HuiPérez Sirkin, Daniela IrinaLafont, Anne GaëlleDe Flori, CélineVissio, Paula GabrielaMazan, SylvieDufour, SylvieVenkatesh, ByrappaTostivint, HervéCARTILAGINOUS FISHELASMOBRANCHIIEVOLUTIONGONADOTROPIN-RELEASING HORMONEHOLOCEPHALIMULTIGENIC FAMILYNEUROPEPTIDESVERTEBRATEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The neuropeptide gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Vertebrates possess multiple GnRH forms that are classified into three main groups, namely GnRH1, GnRH2, and GnRH3. In order to gain more insights into the GnRH gene family in vertebrates, we sought to identify which paralogs of this family are present in cartilaginous fish. For this purpose, we searched the genomes and/or transcriptomes of three representative species of this group, the small-spotted catshark, Scyliorhinus canicula, the whale shark, Rhincodon typus and the elephant shark Callorhinchus milii. In each species, we report the identification of three GnRH genes. In catshark and whale shark, phylogenetic and synteny analysis showed that these three genes correspond to GnRH1, GnRH2, and GnRH3. In both species, GnRH1 was found to encode a novel form of GnRH whose primary structure was determined as follows: QHWSFDLRPG. In elephant shark, the three genes correspond to GnRH1a and GnRH1b, two copies of the GnRH1 gene, plus GnRH2. 3D structure prediction of the chondrichthyan GnRH-associated peptides (GAPs) revealed that catshark GAP1, GAP2, and elephant shark GAP2 peptides exhibit a helix-loop-helix (HLH) structure. This structure observed for many osteichthyan GAP1 and GAP2, may convey GAP biological activity. This HLH structure could not be observed for elephant shark GAP1a and GAP1b. As for all other GAP3 described so far, no typical 3D HLH structure was observed for catshark nor whale shark GAP3. RT-PCR analysis revealed that GnRH1, GnRH2, and GnRH3 genes are differentially expressed in the catshark brain. GnRH1 mRNA appeared predominant in the diencephalon while GnRH2 and GnRH3 mRNAs seemed to be most abundant in the mesencephalon and telencephalon, respectively. Taken together, our results show that the GnRH gene repertoire of the vertebrate ancestor was entirely conserved in the chondrichthyan lineage but that the GnRH3 gene was probably lost in holocephali. They also suggest that the three GnRH neuronal systems previously described in the brain of bony vertebrates are also present in cartilaginous fish.Fil: Gaillard, Anne Laure. Centre National de la Recherche Scientifique; Francia. Muséum National d'Histoire Naturelle; FranciaFil: Tay, Boon Hui. A∗STAR. Institute of Molecular and Cell Biology; SingapurFil: Pérez Sirkin, Daniela Irina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Lafont, Anne Gaëlle. Muséum National d'Histoire Naturelle; Francia. Centre National de la Recherche Scientifique; FranciaFil: De Flori, Céline. Centre National de la Recherche Scientifique; Francia. Muséum National d'Histoire Naturelle; FranciaFil: Vissio, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Mazan, Sylvie. Observatoire Océanlogique de Banyuls, Sorbonne Universi; FranciaFil: Dufour, Sylvie. Muséum National d'Histoire Naturelle; Francia. Centre National de la Recherche Scientifique; Francia. Universite de la Sorbona Nouvelle; FranciaFil: Venkatesh, Byrappa. A∗STAR. Institute of Molecular and Cell Biology; SingapurFil: Tostivint, Hervé. Muséum National d'Histoire Naturelle; Francia. Centre National de la Recherche Scientifique; FranciaFrontiers Media S.A.2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96226Gaillard, Anne Laure; Tay, Boon Hui; Pérez Sirkin, Daniela Irina; Lafont, Anne Gaëlle; De Flori, Céline; et al.; Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives; Frontiers Media S.A.; Frontiers in Neuroscience; 12; 9-2018; 1-121662-453XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fnins.2018.00607info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fnins.2018.00607/fullinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135963/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:31Zoai:ri.conicet.gov.ar:11336/96226instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:31.857CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives
title Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives
spellingShingle Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives
Gaillard, Anne Laure
CARTILAGINOUS FISH
ELASMOBRANCHII
EVOLUTION
GONADOTROPIN-RELEASING HORMONE
HOLOCEPHALI
MULTIGENIC FAMILY
NEUROPEPTIDES
VERTEBRATES
title_short Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives
title_full Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives
title_fullStr Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives
title_full_unstemmed Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives
title_sort Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives
dc.creator.none.fl_str_mv Gaillard, Anne Laure
Tay, Boon Hui
Pérez Sirkin, Daniela Irina
Lafont, Anne Gaëlle
De Flori, Céline
Vissio, Paula Gabriela
Mazan, Sylvie
Dufour, Sylvie
Venkatesh, Byrappa
Tostivint, Hervé
author Gaillard, Anne Laure
author_facet Gaillard, Anne Laure
Tay, Boon Hui
Pérez Sirkin, Daniela Irina
Lafont, Anne Gaëlle
De Flori, Céline
Vissio, Paula Gabriela
Mazan, Sylvie
Dufour, Sylvie
Venkatesh, Byrappa
Tostivint, Hervé
author_role author
author2 Tay, Boon Hui
Pérez Sirkin, Daniela Irina
Lafont, Anne Gaëlle
De Flori, Céline
Vissio, Paula Gabriela
Mazan, Sylvie
Dufour, Sylvie
Venkatesh, Byrappa
Tostivint, Hervé
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CARTILAGINOUS FISH
ELASMOBRANCHII
EVOLUTION
GONADOTROPIN-RELEASING HORMONE
HOLOCEPHALI
MULTIGENIC FAMILY
NEUROPEPTIDES
VERTEBRATES
topic CARTILAGINOUS FISH
ELASMOBRANCHII
EVOLUTION
GONADOTROPIN-RELEASING HORMONE
HOLOCEPHALI
MULTIGENIC FAMILY
NEUROPEPTIDES
VERTEBRATES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The neuropeptide gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Vertebrates possess multiple GnRH forms that are classified into three main groups, namely GnRH1, GnRH2, and GnRH3. In order to gain more insights into the GnRH gene family in vertebrates, we sought to identify which paralogs of this family are present in cartilaginous fish. For this purpose, we searched the genomes and/or transcriptomes of three representative species of this group, the small-spotted catshark, Scyliorhinus canicula, the whale shark, Rhincodon typus and the elephant shark Callorhinchus milii. In each species, we report the identification of three GnRH genes. In catshark and whale shark, phylogenetic and synteny analysis showed that these three genes correspond to GnRH1, GnRH2, and GnRH3. In both species, GnRH1 was found to encode a novel form of GnRH whose primary structure was determined as follows: QHWSFDLRPG. In elephant shark, the three genes correspond to GnRH1a and GnRH1b, two copies of the GnRH1 gene, plus GnRH2. 3D structure prediction of the chondrichthyan GnRH-associated peptides (GAPs) revealed that catshark GAP1, GAP2, and elephant shark GAP2 peptides exhibit a helix-loop-helix (HLH) structure. This structure observed for many osteichthyan GAP1 and GAP2, may convey GAP biological activity. This HLH structure could not be observed for elephant shark GAP1a and GAP1b. As for all other GAP3 described so far, no typical 3D HLH structure was observed for catshark nor whale shark GAP3. RT-PCR analysis revealed that GnRH1, GnRH2, and GnRH3 genes are differentially expressed in the catshark brain. GnRH1 mRNA appeared predominant in the diencephalon while GnRH2 and GnRH3 mRNAs seemed to be most abundant in the mesencephalon and telencephalon, respectively. Taken together, our results show that the GnRH gene repertoire of the vertebrate ancestor was entirely conserved in the chondrichthyan lineage but that the GnRH3 gene was probably lost in holocephali. They also suggest that the three GnRH neuronal systems previously described in the brain of bony vertebrates are also present in cartilaginous fish.
Fil: Gaillard, Anne Laure. Centre National de la Recherche Scientifique; Francia. Muséum National d'Histoire Naturelle; Francia
Fil: Tay, Boon Hui. A∗STAR. Institute of Molecular and Cell Biology; Singapur
Fil: Pérez Sirkin, Daniela Irina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Lafont, Anne Gaëlle. Muséum National d'Histoire Naturelle; Francia. Centre National de la Recherche Scientifique; Francia
Fil: De Flori, Céline. Centre National de la Recherche Scientifique; Francia. Muséum National d'Histoire Naturelle; Francia
Fil: Vissio, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Mazan, Sylvie. Observatoire Océanlogique de Banyuls, Sorbonne Universi; Francia
Fil: Dufour, Sylvie. Muséum National d'Histoire Naturelle; Francia. Centre National de la Recherche Scientifique; Francia. Universite de la Sorbona Nouvelle; Francia
Fil: Venkatesh, Byrappa. A∗STAR. Institute of Molecular and Cell Biology; Singapur
Fil: Tostivint, Hervé. Muséum National d'Histoire Naturelle; Francia. Centre National de la Recherche Scientifique; Francia
description The neuropeptide gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Vertebrates possess multiple GnRH forms that are classified into three main groups, namely GnRH1, GnRH2, and GnRH3. In order to gain more insights into the GnRH gene family in vertebrates, we sought to identify which paralogs of this family are present in cartilaginous fish. For this purpose, we searched the genomes and/or transcriptomes of three representative species of this group, the small-spotted catshark, Scyliorhinus canicula, the whale shark, Rhincodon typus and the elephant shark Callorhinchus milii. In each species, we report the identification of three GnRH genes. In catshark and whale shark, phylogenetic and synteny analysis showed that these three genes correspond to GnRH1, GnRH2, and GnRH3. In both species, GnRH1 was found to encode a novel form of GnRH whose primary structure was determined as follows: QHWSFDLRPG. In elephant shark, the three genes correspond to GnRH1a and GnRH1b, two copies of the GnRH1 gene, plus GnRH2. 3D structure prediction of the chondrichthyan GnRH-associated peptides (GAPs) revealed that catshark GAP1, GAP2, and elephant shark GAP2 peptides exhibit a helix-loop-helix (HLH) structure. This structure observed for many osteichthyan GAP1 and GAP2, may convey GAP biological activity. This HLH structure could not be observed for elephant shark GAP1a and GAP1b. As for all other GAP3 described so far, no typical 3D HLH structure was observed for catshark nor whale shark GAP3. RT-PCR analysis revealed that GnRH1, GnRH2, and GnRH3 genes are differentially expressed in the catshark brain. GnRH1 mRNA appeared predominant in the diencephalon while GnRH2 and GnRH3 mRNAs seemed to be most abundant in the mesencephalon and telencephalon, respectively. Taken together, our results show that the GnRH gene repertoire of the vertebrate ancestor was entirely conserved in the chondrichthyan lineage but that the GnRH3 gene was probably lost in holocephali. They also suggest that the three GnRH neuronal systems previously described in the brain of bony vertebrates are also present in cartilaginous fish.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96226
Gaillard, Anne Laure; Tay, Boon Hui; Pérez Sirkin, Daniela Irina; Lafont, Anne Gaëlle; De Flori, Céline; et al.; Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives; Frontiers Media S.A.; Frontiers in Neuroscience; 12; 9-2018; 1-12
1662-453X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96226
identifier_str_mv Gaillard, Anne Laure; Tay, Boon Hui; Pérez Sirkin, Daniela Irina; Lafont, Anne Gaëlle; De Flori, Céline; et al.; Characterization of Gonadotropin-Releasing Hormone (GnRH) Genes From Cartilaginous Fish: Evolutionary Perspectives; Frontiers Media S.A.; Frontiers in Neuroscience; 12; 9-2018; 1-12
1662-453X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fnins.2018.00607
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fnins.2018.00607/full
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135963/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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