DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration

Autores
Martínez Vivot, Rocío; Copello, Guillermo Javier; Leal, Maria Celeste; Piñero, Gonzalo Miguel; Usach, Vanina; Rozenszajn, Mijael; Morelli, Laura; Setton, Clara Patricia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous work by our group demonstrated the key role of iron in Schwann cell (SC)maturation through an increase in cAMP, PKA activation and CREB phosphorylation. These studies opened the door to further research on a non-transferrin-bound iron uptake, which revealed the presence of DMT1 mRNA all along SC progeny, hinting at a constitutive role of DMT1 in ensuring the provision of iron in the PNS. In light of these previous results,the present work evaluates the participation of DMT1 in the remyelination process following a demyelinating lesion promoted by sciatic nerve crush ?a reversible model of Wallerian degeneration. DMT1 was observed to colocalize with SC marker S100β at all survival times analyzed. In turn, the assessment of DMT1 mRNA expression exhibited an increase 7 days post-injury, while DMT1 protein levels showed an increase 14 days after crush at the lesion site and distal stump; finally, an increase in iron levels became evidentas from 14 days post-injury, in parallel with DMT1 values. To sum up, the present work unveils the role of DMT1 in mediating the neuroregenerative action of iron.
Fil: Martínez Vivot, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Copello, Guillermo Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina
Fil: Leal, Maria Celeste. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Piñero, Gonzalo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Usach, Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Rozenszajn, Mijael. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Morelli, Laura. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Setton, Clara Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Materia
Schwann Cell
Non-Transferrin-Bound Iron Uptake
Dmt1
Remyelination Process
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11628
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11628
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling DMT1 iron uptake in the PNS: bridging the gap between injury and regenerationMartínez Vivot, RocíoCopello, Guillermo JavierLeal, Maria CelestePiñero, Gonzalo MiguelUsach, VaninaRozenszajn, MijaelMorelli, LauraSetton, Clara PatriciaSchwann CellNon-Transferrin-Bound Iron UptakeDmt1Remyelination Processhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Previous work by our group demonstrated the key role of iron in Schwann cell (SC)maturation through an increase in cAMP, PKA activation and CREB phosphorylation. These studies opened the door to further research on a non-transferrin-bound iron uptake, which revealed the presence of DMT1 mRNA all along SC progeny, hinting at a constitutive role of DMT1 in ensuring the provision of iron in the PNS. In light of these previous results,the present work evaluates the participation of DMT1 in the remyelination process following a demyelinating lesion promoted by sciatic nerve crush ?a reversible model of Wallerian degeneration. DMT1 was observed to colocalize with SC marker S100β at all survival times analyzed. In turn, the assessment of DMT1 mRNA expression exhibited an increase 7 days post-injury, while DMT1 protein levels showed an increase 14 days after crush at the lesion site and distal stump; finally, an increase in iron levels became evidentas from 14 days post-injury, in parallel with DMT1 values. To sum up, the present work unveils the role of DMT1 in mediating the neuroregenerative action of iron.Fil: Martínez Vivot, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; ArgentinaFil: Copello, Guillermo Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; ArgentinaFil: Leal, Maria Celeste. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Piñero, Gonzalo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; ArgentinaFil: Usach, Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; ArgentinaFil: Rozenszajn, Mijael. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; ArgentinaFil: Morelli, Laura. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Setton, Clara Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; ArgentinaRoyal Society Of Chemistry2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11628Martínez Vivot, Rocío; Copello, Guillermo Javier; Leal, Maria Celeste; Piñero, Gonzalo Miguel; Usach, Vanina; et al.; DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration; Royal Society Of Chemistry; Metallomics; 7; 10; 9-2015; 1381-13891756-5901enginfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2015/MT/C5MT00156K#!divAbstractinfo:eu-repo/semantics/altIdentifier/doi/10.1039/C5MT00156Kinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:59Zoai:ri.conicet.gov.ar:11336/11628instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:59.802CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration
title DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration
spellingShingle DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration
Martínez Vivot, Rocío
Schwann Cell
Non-Transferrin-Bound Iron Uptake
Dmt1
Remyelination Process
title_short DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration
title_full DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration
title_fullStr DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration
title_full_unstemmed DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration
title_sort DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration
dc.creator.none.fl_str_mv Martínez Vivot, Rocío
Copello, Guillermo Javier
Leal, Maria Celeste
Piñero, Gonzalo Miguel
Usach, Vanina
Rozenszajn, Mijael
Morelli, Laura
Setton, Clara Patricia
author Martínez Vivot, Rocío
author_facet Martínez Vivot, Rocío
Copello, Guillermo Javier
Leal, Maria Celeste
Piñero, Gonzalo Miguel
Usach, Vanina
Rozenszajn, Mijael
Morelli, Laura
Setton, Clara Patricia
author_role author
author2 Copello, Guillermo Javier
Leal, Maria Celeste
Piñero, Gonzalo Miguel
Usach, Vanina
Rozenszajn, Mijael
Morelli, Laura
Setton, Clara Patricia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Schwann Cell
Non-Transferrin-Bound Iron Uptake
Dmt1
Remyelination Process
topic Schwann Cell
Non-Transferrin-Bound Iron Uptake
Dmt1
Remyelination Process
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Previous work by our group demonstrated the key role of iron in Schwann cell (SC)maturation through an increase in cAMP, PKA activation and CREB phosphorylation. These studies opened the door to further research on a non-transferrin-bound iron uptake, which revealed the presence of DMT1 mRNA all along SC progeny, hinting at a constitutive role of DMT1 in ensuring the provision of iron in the PNS. In light of these previous results,the present work evaluates the participation of DMT1 in the remyelination process following a demyelinating lesion promoted by sciatic nerve crush ?a reversible model of Wallerian degeneration. DMT1 was observed to colocalize with SC marker S100β at all survival times analyzed. In turn, the assessment of DMT1 mRNA expression exhibited an increase 7 days post-injury, while DMT1 protein levels showed an increase 14 days after crush at the lesion site and distal stump; finally, an increase in iron levels became evidentas from 14 days post-injury, in parallel with DMT1 values. To sum up, the present work unveils the role of DMT1 in mediating the neuroregenerative action of iron.
Fil: Martínez Vivot, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Copello, Guillermo Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina
Fil: Leal, Maria Celeste. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Piñero, Gonzalo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Usach, Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Rozenszajn, Mijael. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Morelli, Laura. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Setton, Clara Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
description Previous work by our group demonstrated the key role of iron in Schwann cell (SC)maturation through an increase in cAMP, PKA activation and CREB phosphorylation. These studies opened the door to further research on a non-transferrin-bound iron uptake, which revealed the presence of DMT1 mRNA all along SC progeny, hinting at a constitutive role of DMT1 in ensuring the provision of iron in the PNS. In light of these previous results,the present work evaluates the participation of DMT1 in the remyelination process following a demyelinating lesion promoted by sciatic nerve crush ?a reversible model of Wallerian degeneration. DMT1 was observed to colocalize with SC marker S100β at all survival times analyzed. In turn, the assessment of DMT1 mRNA expression exhibited an increase 7 days post-injury, while DMT1 protein levels showed an increase 14 days after crush at the lesion site and distal stump; finally, an increase in iron levels became evidentas from 14 days post-injury, in parallel with DMT1 values. To sum up, the present work unveils the role of DMT1 in mediating the neuroregenerative action of iron.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11628
Martínez Vivot, Rocío; Copello, Guillermo Javier; Leal, Maria Celeste; Piñero, Gonzalo Miguel; Usach, Vanina; et al.; DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration; Royal Society Of Chemistry; Metallomics; 7; 10; 9-2015; 1381-1389
1756-5901
url http://hdl.handle.net/11336/11628
identifier_str_mv Martínez Vivot, Rocío; Copello, Guillermo Javier; Leal, Maria Celeste; Piñero, Gonzalo Miguel; Usach, Vanina; et al.; DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration; Royal Society Of Chemistry; Metallomics; 7; 10; 9-2015; 1381-1389
1756-5901
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2015/MT/C5MT00156K#!divAbstract
info:eu-repo/semantics/altIdentifier/doi/10.1039/C5MT00156K
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society Of Chemistry
publisher.none.fl_str_mv Royal Society Of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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