Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells

Autores
Pérez, G.; Pregi, N.; Vittori, D.; Di Risio, C.; Garbossa, G.; Nesse, A.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aluminum (Al) and iron (Fe) share several physicochemical characteristics and they both bind to transferrin (Tf), entering the cell via Tf receptors (TfR). Previously, we found similar values of affinity constant for the binding of TfR to Tf carrying either Al or Fe. The competitive interaction between both metals prevented normal Fe incorporation into K562 cells and triggered the upregulation of Fe transport. In the present work we demonstrated that Al modified Fe uptake without affecting the expression of Tf receptors. Both TfR and TfR2 mRNA levels, evaluated by RT-PCR, and TfR antigenic sites, analyzed by flow cytometry, were found unchanged after Al exposure. In turn, Al did induce upregulation of non-Tf bound Fe (NTBI) uptake. This modulation was not due to intracellular Fe decrease since NTBI transport proved not to be regulated by Fe depletion. Unlike its behavior in the presence of Tf, Al was unable to compete with NTBI uptake, suggesting that both metals do not share the same alternative transport pathway. We propose that Al interference with TfR-mediated Fe incorporation might trigger the upregulation of NTBI uptake, an adaptation aimed at incorporating the essential metal required for cellular metabolism without allowing the simultaneous access of a potentially toxic metal. © 2004 Elsevier B.V. All rights reserved.
Fil:Pérez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Vittori, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Di Risio, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Garbossa, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Biochim. Biophys. Acta Mol. Cell Res. 2005;1745(1):124-130
Materia
Aluminum
Iron metabolism
K562 cell line
Non-transferrin bound iron transport
Transferrin receptor
Transferrin-mediated iron uptake
aluminum
antigen
iron
messenger RNA
receptor subtype
transferrin
transferrin receptor
article
chronic myeloid leukemia
controlled study
flow cytometry
human
human cell
iron depletion
iron transport
leukemia cell
priority journal
protein expression
receptor binding
regulatory mechanism
reverse transcription polymerase chain reaction
upregulation
Aluminum
Biological Transport
Butyrates
Cell Differentiation
Gene Expression Regulation, Neoplastic
Humans
Iron
K562 Cells
Receptors, Transferrin
RNA, Messenger
Transferrin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_01674889_v1745_n1_p124_Perez
Acceder
id BDUBAFCEN_ab38c42fc1176f67f98cac99c211036f
oai_identifier_str paperaa:paper_01674889_v1745_n1_p124_Perez
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cellsPérez, G.Pregi, N.Vittori, D.Di Risio, C.Garbossa, G.Nesse, A.AluminumIron metabolismK562 cell lineNon-transferrin bound iron transportTransferrin receptorTransferrin-mediated iron uptakealuminumantigenironmessenger RNAreceptor subtypetransferrintransferrin receptorarticlechronic myeloid leukemiacontrolled studyflow cytometryhumanhuman celliron depletioniron transportleukemia cellpriority journalprotein expressionreceptor bindingregulatory mechanismreverse transcription polymerase chain reactionupregulationAluminumBiological TransportButyratesCell DifferentiationGene Expression Regulation, NeoplasticHumansIronK562 CellsReceptors, TransferrinRNA, MessengerTransferrinAluminum (Al) and iron (Fe) share several physicochemical characteristics and they both bind to transferrin (Tf), entering the cell via Tf receptors (TfR). Previously, we found similar values of affinity constant for the binding of TfR to Tf carrying either Al or Fe. The competitive interaction between both metals prevented normal Fe incorporation into K562 cells and triggered the upregulation of Fe transport. In the present work we demonstrated that Al modified Fe uptake without affecting the expression of Tf receptors. Both TfR and TfR2 mRNA levels, evaluated by RT-PCR, and TfR antigenic sites, analyzed by flow cytometry, were found unchanged after Al exposure. In turn, Al did induce upregulation of non-Tf bound Fe (NTBI) uptake. This modulation was not due to intracellular Fe decrease since NTBI transport proved not to be regulated by Fe depletion. Unlike its behavior in the presence of Tf, Al was unable to compete with NTBI uptake, suggesting that both metals do not share the same alternative transport pathway. We propose that Al interference with TfR-mediated Fe incorporation might trigger the upregulation of NTBI uptake, an adaptation aimed at incorporating the essential metal required for cellular metabolism without allowing the simultaneous access of a potentially toxic metal. © 2004 Elsevier B.V. All rights reserved.Fil:Pérez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Vittori, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Di Risio, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Garbossa, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_01674889_v1745_n1_p124_PerezBiochim. Biophys. Acta Mol. Cell Res. 2005;1745(1):124-130reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:05Zpaperaa:paper_01674889_v1745_n1_p124_PerezInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:06.953Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
title Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
spellingShingle Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
Pérez, G.
Aluminum
Iron metabolism
K562 cell line
Non-transferrin bound iron transport
Transferrin receptor
Transferrin-mediated iron uptake
aluminum
antigen
iron
messenger RNA
receptor subtype
transferrin
transferrin receptor
article
chronic myeloid leukemia
controlled study
flow cytometry
human
human cell
iron depletion
iron transport
leukemia cell
priority journal
protein expression
receptor binding
regulatory mechanism
reverse transcription polymerase chain reaction
upregulation
Aluminum
Biological Transport
Butyrates
Cell Differentiation
Gene Expression Regulation, Neoplastic
Humans
Iron
K562 Cells
Receptors, Transferrin
RNA, Messenger
Transferrin
title_short Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
title_full Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
title_fullStr Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
title_full_unstemmed Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
title_sort Aluminum exposure affects transferrin-dependent and -independent iron uptake by K562 cells
dc.creator.none.fl_str_mv Pérez, G.
Pregi, N.
Vittori, D.
Di Risio, C.
Garbossa, G.
Nesse, A.
author Pérez, G.
author_facet Pérez, G.
Pregi, N.
Vittori, D.
Di Risio, C.
Garbossa, G.
Nesse, A.
author_role author
author2 Pregi, N.
Vittori, D.
Di Risio, C.
Garbossa, G.
Nesse, A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Aluminum
Iron metabolism
K562 cell line
Non-transferrin bound iron transport
Transferrin receptor
Transferrin-mediated iron uptake
aluminum
antigen
iron
messenger RNA
receptor subtype
transferrin
transferrin receptor
article
chronic myeloid leukemia
controlled study
flow cytometry
human
human cell
iron depletion
iron transport
leukemia cell
priority journal
protein expression
receptor binding
regulatory mechanism
reverse transcription polymerase chain reaction
upregulation
Aluminum
Biological Transport
Butyrates
Cell Differentiation
Gene Expression Regulation, Neoplastic
Humans
Iron
K562 Cells
Receptors, Transferrin
RNA, Messenger
Transferrin
topic Aluminum
Iron metabolism
K562 cell line
Non-transferrin bound iron transport
Transferrin receptor
Transferrin-mediated iron uptake
aluminum
antigen
iron
messenger RNA
receptor subtype
transferrin
transferrin receptor
article
chronic myeloid leukemia
controlled study
flow cytometry
human
human cell
iron depletion
iron transport
leukemia cell
priority journal
protein expression
receptor binding
regulatory mechanism
reverse transcription polymerase chain reaction
upregulation
Aluminum
Biological Transport
Butyrates
Cell Differentiation
Gene Expression Regulation, Neoplastic
Humans
Iron
K562 Cells
Receptors, Transferrin
RNA, Messenger
Transferrin
dc.description.none.fl_txt_mv Aluminum (Al) and iron (Fe) share several physicochemical characteristics and they both bind to transferrin (Tf), entering the cell via Tf receptors (TfR). Previously, we found similar values of affinity constant for the binding of TfR to Tf carrying either Al or Fe. The competitive interaction between both metals prevented normal Fe incorporation into K562 cells and triggered the upregulation of Fe transport. In the present work we demonstrated that Al modified Fe uptake without affecting the expression of Tf receptors. Both TfR and TfR2 mRNA levels, evaluated by RT-PCR, and TfR antigenic sites, analyzed by flow cytometry, were found unchanged after Al exposure. In turn, Al did induce upregulation of non-Tf bound Fe (NTBI) uptake. This modulation was not due to intracellular Fe decrease since NTBI transport proved not to be regulated by Fe depletion. Unlike its behavior in the presence of Tf, Al was unable to compete with NTBI uptake, suggesting that both metals do not share the same alternative transport pathway. We propose that Al interference with TfR-mediated Fe incorporation might trigger the upregulation of NTBI uptake, an adaptation aimed at incorporating the essential metal required for cellular metabolism without allowing the simultaneous access of a potentially toxic metal. © 2004 Elsevier B.V. All rights reserved.
Fil:Pérez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Vittori, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Di Risio, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Garbossa, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Aluminum (Al) and iron (Fe) share several physicochemical characteristics and they both bind to transferrin (Tf), entering the cell via Tf receptors (TfR). Previously, we found similar values of affinity constant for the binding of TfR to Tf carrying either Al or Fe. The competitive interaction between both metals prevented normal Fe incorporation into K562 cells and triggered the upregulation of Fe transport. In the present work we demonstrated that Al modified Fe uptake without affecting the expression of Tf receptors. Both TfR and TfR2 mRNA levels, evaluated by RT-PCR, and TfR antigenic sites, analyzed by flow cytometry, were found unchanged after Al exposure. In turn, Al did induce upregulation of non-Tf bound Fe (NTBI) uptake. This modulation was not due to intracellular Fe decrease since NTBI transport proved not to be regulated by Fe depletion. Unlike its behavior in the presence of Tf, Al was unable to compete with NTBI uptake, suggesting that both metals do not share the same alternative transport pathway. We propose that Al interference with TfR-mediated Fe incorporation might trigger the upregulation of NTBI uptake, an adaptation aimed at incorporating the essential metal required for cellular metabolism without allowing the simultaneous access of a potentially toxic metal. © 2004 Elsevier B.V. All rights reserved.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_01674889_v1745_n1_p124_Perez
url http://hdl.handle.net/20.500.12110/paper_01674889_v1745_n1_p124_Perez
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Biochim. Biophys. Acta Mol. Cell Res. 2005;1745(1):124-130
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 13.070432

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