Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease

Autores
Fernandez Delias, María Florencia; Roque, Marta Elena
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The prevalence of iron overload cardiomyopathy is increasing. There is growing evidence that high iron levels are a risk factor for cardiovascular disease because can cause constriction of blood vessels.The aim was to study the erythropoietin (EPO) role to prevent iron induced cardiovascular disease studying key importer proteins in the heart in an animal model of iron overload and EPO. CF1 mice (25±5g; 3 months) were divided into groups (n=4/group):1)Iron-adequate (IA); 2)Iron-overload (IO) (iron saccharate; days 0,4,8,12 ip;1800mg/kg); 3)EPO (days17,18,19) ip; 20000UI/kg); 4)Iron-overload+EPO (IO+EPO). Immunohistochemistry:anti-DMT1(divalent metal transporter1) and ZIP14(Zrt-Irt-like Protein14).Perl´s staining .Iron levels were measured by FeRcolor.The Protocol was approved by the CICUAE,UNS. Heart DMT1 expression was evident in IA and EPO groups and it was scarce in IO and IO+EPO conditions. However heart ZIP14 expression was evident in all conditions demonstrating that it´s expression not depends of the ?iron signal?. The decrease in the DMT1 expression in IO state would suggest a protective mechanism against iron excess in heart tissue, being the ?iron signal? the predominant signal to decrease the biometal uptake. Iron levels in heart shows significant increase in IO respect to IA condition. Interestingly, the iron levels in IO+EPO were significantly decreased respect to IO. Consequently, abundant hemosiderin was observed in IO condition and it was scarce in IO+EPO group. Hemosiderin was absent in IA and EPO conditions. Our data showed that Iron uptake in IO would not depend on the expression of DMT1 either ZIP14. Thus, we can conclude that erythropoietin the ?EPO signal? in high iron levels may have a direct positive effect on the heart. In conclusion, the interplay between EPO and key proteins of the iron cycle, such as DMT1 and ZIP14 may help to better understand the mechanisms involved in iron and erythropoiesis regulation in heart tissue.
Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
IRON
EPO
HEART
DMT1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/209044
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/209044
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Potencial benefit of erythropoietin to prevent iron induced cardiovascular diseaseFernandez Delias, María FlorenciaRoque, Marta ElenaIRONEPOHEARTDMT1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The prevalence of iron overload cardiomyopathy is increasing. There is growing evidence that high iron levels are a risk factor for cardiovascular disease because can cause constriction of blood vessels.The aim was to study the erythropoietin (EPO) role to prevent iron induced cardiovascular disease studying key importer proteins in the heart in an animal model of iron overload and EPO. CF1 mice (25±5g; 3 months) were divided into groups (n=4/group):1)Iron-adequate (IA); 2)Iron-overload (IO) (iron saccharate; days 0,4,8,12 ip;1800mg/kg); 3)EPO (days17,18,19) ip; 20000UI/kg); 4)Iron-overload+EPO (IO+EPO). Immunohistochemistry:anti-DMT1(divalent metal transporter1) and ZIP14(Zrt-Irt-like Protein14).Perl´s staining .Iron levels were measured by FeRcolor.The Protocol was approved by the CICUAE,UNS. Heart DMT1 expression was evident in IA and EPO groups and it was scarce in IO and IO+EPO conditions. However heart ZIP14 expression was evident in all conditions demonstrating that it´s expression not depends of the ?iron signal?. The decrease in the DMT1 expression in IO state would suggest a protective mechanism against iron excess in heart tissue, being the ?iron signal? the predominant signal to decrease the biometal uptake. Iron levels in heart shows significant increase in IO respect to IA condition. Interestingly, the iron levels in IO+EPO were significantly decreased respect to IO. Consequently, abundant hemosiderin was observed in IO condition and it was scarce in IO+EPO group. Hemosiderin was absent in IA and EPO conditions. Our data showed that Iron uptake in IO would not depend on the expression of DMT1 either ZIP14. Thus, we can conclude that erythropoietin the ?EPO signal? in high iron levels may have a direct positive effect on the heart. In conclusion, the interplay between EPO and key proteins of the iron cycle, such as DMT1 and ZIP14 may help to better understand the mechanisms involved in iron and erythropoiesis regulation in heart tissue.Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaLXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista MedicinaAzurmendi, Pablo Javier2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/209044Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 191-1920025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:42Zoai:ri.conicet.gov.ar:11336/209044instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:43.231CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease
title Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease
spellingShingle Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease
Fernandez Delias, María Florencia
IRON
EPO
HEART
DMT1
title_short Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease
title_full Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease
title_fullStr Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease
title_full_unstemmed Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease
title_sort Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease
dc.creator.none.fl_str_mv Fernandez Delias, María Florencia
Roque, Marta Elena
author Fernandez Delias, María Florencia
author_facet Fernandez Delias, María Florencia
Roque, Marta Elena
author_role author
author2 Roque, Marta Elena
author2_role author
dc.contributor.none.fl_str_mv Azurmendi, Pablo Javier
dc.subject.none.fl_str_mv IRON
EPO
HEART
DMT1
topic IRON
EPO
HEART
DMT1
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The prevalence of iron overload cardiomyopathy is increasing. There is growing evidence that high iron levels are a risk factor for cardiovascular disease because can cause constriction of blood vessels.The aim was to study the erythropoietin (EPO) role to prevent iron induced cardiovascular disease studying key importer proteins in the heart in an animal model of iron overload and EPO. CF1 mice (25±5g; 3 months) were divided into groups (n=4/group):1)Iron-adequate (IA); 2)Iron-overload (IO) (iron saccharate; days 0,4,8,12 ip;1800mg/kg); 3)EPO (days17,18,19) ip; 20000UI/kg); 4)Iron-overload+EPO (IO+EPO). Immunohistochemistry:anti-DMT1(divalent metal transporter1) and ZIP14(Zrt-Irt-like Protein14).Perl´s staining .Iron levels were measured by FeRcolor.The Protocol was approved by the CICUAE,UNS. Heart DMT1 expression was evident in IA and EPO groups and it was scarce in IO and IO+EPO conditions. However heart ZIP14 expression was evident in all conditions demonstrating that it´s expression not depends of the ?iron signal?. The decrease in the DMT1 expression in IO state would suggest a protective mechanism against iron excess in heart tissue, being the ?iron signal? the predominant signal to decrease the biometal uptake. Iron levels in heart shows significant increase in IO respect to IA condition. Interestingly, the iron levels in IO+EPO were significantly decreased respect to IO. Consequently, abundant hemosiderin was observed in IO condition and it was scarce in IO+EPO group. Hemosiderin was absent in IA and EPO conditions. Our data showed that Iron uptake in IO would not depend on the expression of DMT1 either ZIP14. Thus, we can conclude that erythropoietin the ?EPO signal? in high iron levels may have a direct positive effect on the heart. In conclusion, the interplay between EPO and key proteins of the iron cycle, such as DMT1 and ZIP14 may help to better understand the mechanisms involved in iron and erythropoiesis regulation in heart tissue.
Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description The prevalence of iron overload cardiomyopathy is increasing. There is growing evidence that high iron levels are a risk factor for cardiovascular disease because can cause constriction of blood vessels.The aim was to study the erythropoietin (EPO) role to prevent iron induced cardiovascular disease studying key importer proteins in the heart in an animal model of iron overload and EPO. CF1 mice (25±5g; 3 months) were divided into groups (n=4/group):1)Iron-adequate (IA); 2)Iron-overload (IO) (iron saccharate; days 0,4,8,12 ip;1800mg/kg); 3)EPO (days17,18,19) ip; 20000UI/kg); 4)Iron-overload+EPO (IO+EPO). Immunohistochemistry:anti-DMT1(divalent metal transporter1) and ZIP14(Zrt-Irt-like Protein14).Perl´s staining .Iron levels were measured by FeRcolor.The Protocol was approved by the CICUAE,UNS. Heart DMT1 expression was evident in IA and EPO groups and it was scarce in IO and IO+EPO conditions. However heart ZIP14 expression was evident in all conditions demonstrating that it´s expression not depends of the ?iron signal?. The decrease in the DMT1 expression in IO state would suggest a protective mechanism against iron excess in heart tissue, being the ?iron signal? the predominant signal to decrease the biometal uptake. Iron levels in heart shows significant increase in IO respect to IA condition. Interestingly, the iron levels in IO+EPO were significantly decreased respect to IO. Consequently, abundant hemosiderin was observed in IO condition and it was scarce in IO+EPO group. Hemosiderin was absent in IA and EPO conditions. Our data showed that Iron uptake in IO would not depend on the expression of DMT1 either ZIP14. Thus, we can conclude that erythropoietin the ?EPO signal? in high iron levels may have a direct positive effect on the heart. In conclusion, the interplay between EPO and key proteins of the iron cycle, such as DMT1 and ZIP14 may help to better understand the mechanisms involved in iron and erythropoiesis regulation in heart tissue.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/209044
Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 191-192
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/209044
identifier_str_mv Potencial benefit of erythropoietin to prevent iron induced cardiovascular disease; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 191-192
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicina
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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