Effect of erythropoietin in bronchial cells in an iron excess mouse model

Autores
Fernandez Delias, María Florencia; Roque, Marta Elena
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Imbalances of iron homeostasis are implicated in acute and chronic lung diseases. However, the mechanisms involved in pulmonary iron deposition and its role in the pathogenesis of lung diseases remains unknown. The aim was evaluate the effect of erythropoietin on bronquial cells in an iron excess mouse model studying the regulatory proteins of the iron cycle CF1 mice(25±5g; 3 months-old) were divided into 4 groups(n=4/group):1)Control; 2)Iron-overload(iron saccharate;days0,4,8,12 ip;1800mg/kg); 3)EPO(days17,18,19) ip;20000UI/kg);4)Iron-overload+EPO. Immunohistochemistry: anti-prohepcidin, L-ferritin, DMT1(divalent metal transporter1) and ZIP14(Zrt-Irt-like Protein14) followed by Perl´s staining. The Protocol was approved by the CICUAE; UNS.We observe that the DMT1 localization in bronchial cells was cytoplasmatic in iron overload+EPO, control and EPO whille in overload the importer was in the apical zone and in membrane cells.ZIP14 expression in bronchial cells was evident in iron overload while it was slight iron overload+EPO, control and EPO.In control and EPO hemosiderin was absent while in Iron overload and iron overload+EPO it was abundant in alveoli.The L-ferritin expression in iron overload was intense in alveoli and apical in bronchial cells. However it expression in iron overload+EPO was cytoplasmic in bronchial cells. It expression was slight in alveoli and cytoplasmatic in bronchial cells of control and EPO.The prohepcidin expression was similar in all conditions.The decrease of ZIP14 expression, and the change in the DMT1 and L-ferritin localization in Iron overload+EPO compared to iron overload, could be reflecting a lower iron uptake and storage in bronchial cells in EPO presence, suggesting a protective mechanism EPO-DEPENDENT.
Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad y Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad argentina de investigación clínica
Sociedad argentina de inmunología
Sociedad argentina de fisiología
Materia
IRON
BRONCHIAL CELLS
IRON OVERLOAD
DMT1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244499
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/244499
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of erythropoietin in bronchial cells in an iron excess mouse modelFernandez Delias, María FlorenciaRoque, Marta ElenaIRONBRONCHIAL CELLSIRON OVERLOADDMT1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Imbalances of iron homeostasis are implicated in acute and chronic lung diseases. However, the mechanisms involved in pulmonary iron deposition and its role in the pathogenesis of lung diseases remains unknown. The aim was evaluate the effect of erythropoietin on bronquial cells in an iron excess mouse model studying the regulatory proteins of the iron cycle CF1 mice(25±5g; 3 months-old) were divided into 4 groups(n=4/group):1)Control; 2)Iron-overload(iron saccharate;days0,4,8,12 ip;1800mg/kg); 3)EPO(days17,18,19) ip;20000UI/kg);4)Iron-overload+EPO. Immunohistochemistry: anti-prohepcidin, L-ferritin, DMT1(divalent metal transporter1) and ZIP14(Zrt-Irt-like Protein14) followed by Perl´s staining. The Protocol was approved by the CICUAE; UNS.We observe that the DMT1 localization in bronchial cells was cytoplasmatic in iron overload+EPO, control and EPO whille in overload the importer was in the apical zone and in membrane cells.ZIP14 expression in bronchial cells was evident in iron overload while it was slight iron overload+EPO, control and EPO.In control and EPO hemosiderin was absent while in Iron overload and iron overload+EPO it was abundant in alveoli.The L-ferritin expression in iron overload was intense in alveoli and apical in bronchial cells. However it expression in iron overload+EPO was cytoplasmic in bronchial cells. It expression was slight in alveoli and cytoplasmatic in bronchial cells of control and EPO.The prohepcidin expression was similar in all conditions.The decrease of ZIP14 expression, and the change in the DMT1 and L-ferritin localization in Iron overload+EPO compared to iron overload, could be reflecting a lower iron uptake and storage in bronchial cells in EPO presence, suggesting a protective mechanism EPO-DEPENDENT.Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaLXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad y Reunión Anual de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad argentina de investigación clínicaSociedad argentina de inmunologíaSociedad argentina de fisiologíaFundación Revista Medicina2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244499Effect of erythropoietin in bronchial cells in an iron excess mouse model; LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 252-2520025-76801669-9106CONICET DigitalCONICETenghttps://www.saic.org.ar/reuniones-anuales-previasinfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:15Zoai:ri.conicet.gov.ar:11336/244499instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:15.681CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of erythropoietin in bronchial cells in an iron excess mouse model
title Effect of erythropoietin in bronchial cells in an iron excess mouse model
spellingShingle Effect of erythropoietin in bronchial cells in an iron excess mouse model
Fernandez Delias, María Florencia
IRON
BRONCHIAL CELLS
IRON OVERLOAD
DMT1
title_short Effect of erythropoietin in bronchial cells in an iron excess mouse model
title_full Effect of erythropoietin in bronchial cells in an iron excess mouse model
title_fullStr Effect of erythropoietin in bronchial cells in an iron excess mouse model
title_full_unstemmed Effect of erythropoietin in bronchial cells in an iron excess mouse model
title_sort Effect of erythropoietin in bronchial cells in an iron excess mouse model
dc.creator.none.fl_str_mv Fernandez Delias, María Florencia
Roque, Marta Elena
author Fernandez Delias, María Florencia
author_facet Fernandez Delias, María Florencia
Roque, Marta Elena
author_role author
author2 Roque, Marta Elena
author2_role author
dc.subject.none.fl_str_mv IRON
BRONCHIAL CELLS
IRON OVERLOAD
DMT1
topic IRON
BRONCHIAL CELLS
IRON OVERLOAD
DMT1
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Imbalances of iron homeostasis are implicated in acute and chronic lung diseases. However, the mechanisms involved in pulmonary iron deposition and its role in the pathogenesis of lung diseases remains unknown. The aim was evaluate the effect of erythropoietin on bronquial cells in an iron excess mouse model studying the regulatory proteins of the iron cycle CF1 mice(25±5g; 3 months-old) were divided into 4 groups(n=4/group):1)Control; 2)Iron-overload(iron saccharate;days0,4,8,12 ip;1800mg/kg); 3)EPO(days17,18,19) ip;20000UI/kg);4)Iron-overload+EPO. Immunohistochemistry: anti-prohepcidin, L-ferritin, DMT1(divalent metal transporter1) and ZIP14(Zrt-Irt-like Protein14) followed by Perl´s staining. The Protocol was approved by the CICUAE; UNS.We observe that the DMT1 localization in bronchial cells was cytoplasmatic in iron overload+EPO, control and EPO whille in overload the importer was in the apical zone and in membrane cells.ZIP14 expression in bronchial cells was evident in iron overload while it was slight iron overload+EPO, control and EPO.In control and EPO hemosiderin was absent while in Iron overload and iron overload+EPO it was abundant in alveoli.The L-ferritin expression in iron overload was intense in alveoli and apical in bronchial cells. However it expression in iron overload+EPO was cytoplasmic in bronchial cells. It expression was slight in alveoli and cytoplasmatic in bronchial cells of control and EPO.The prohepcidin expression was similar in all conditions.The decrease of ZIP14 expression, and the change in the DMT1 and L-ferritin localization in Iron overload+EPO compared to iron overload, could be reflecting a lower iron uptake and storage in bronchial cells in EPO presence, suggesting a protective mechanism EPO-DEPENDENT.
Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad y Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad argentina de investigación clínica
Sociedad argentina de inmunología
Sociedad argentina de fisiología
description Imbalances of iron homeostasis are implicated in acute and chronic lung diseases. However, the mechanisms involved in pulmonary iron deposition and its role in the pathogenesis of lung diseases remains unknown. The aim was evaluate the effect of erythropoietin on bronquial cells in an iron excess mouse model studying the regulatory proteins of the iron cycle CF1 mice(25±5g; 3 months-old) were divided into 4 groups(n=4/group):1)Control; 2)Iron-overload(iron saccharate;days0,4,8,12 ip;1800mg/kg); 3)EPO(days17,18,19) ip;20000UI/kg);4)Iron-overload+EPO. Immunohistochemistry: anti-prohepcidin, L-ferritin, DMT1(divalent metal transporter1) and ZIP14(Zrt-Irt-like Protein14) followed by Perl´s staining. The Protocol was approved by the CICUAE; UNS.We observe that the DMT1 localization in bronchial cells was cytoplasmatic in iron overload+EPO, control and EPO whille in overload the importer was in the apical zone and in membrane cells.ZIP14 expression in bronchial cells was evident in iron overload while it was slight iron overload+EPO, control and EPO.In control and EPO hemosiderin was absent while in Iron overload and iron overload+EPO it was abundant in alveoli.The L-ferritin expression in iron overload was intense in alveoli and apical in bronchial cells. However it expression in iron overload+EPO was cytoplasmic in bronchial cells. It expression was slight in alveoli and cytoplasmatic in bronchial cells of control and EPO.The prohepcidin expression was similar in all conditions.The decrease of ZIP14 expression, and the change in the DMT1 and L-ferritin localization in Iron overload+EPO compared to iron overload, could be reflecting a lower iron uptake and storage in bronchial cells in EPO presence, suggesting a protective mechanism EPO-DEPENDENT.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244499
Effect of erythropoietin in bronchial cells in an iron excess mouse model; LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 252-252
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244499
identifier_str_mv Effect of erythropoietin in bronchial cells in an iron excess mouse model; LXIII Reunión Anual de la de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 252-252
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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