Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption

Autores
Garland, J. L.; Zabaloy, Maria Celina; Birmele, M.; Mackowiak, C. L.; Lehman, R. M.; Frey, S. D.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nitrogen-limited soil microbial activity has important implications for soil carbon storage and nutrient availability, but previous methods for assessing resource limitation have been restricted, due to enrichment criteria (i.e., long incubation periods, high substrate amendments) and/or logistical constraints (e.g. use of radioisotopes). A microtiter-based assay of basal and substrate induced soil respiration based on O2 consumption may be a rapid, ecologically relevant means of assessing N limitation. The present study evaluated this approach by examining 1) the extent and duration of N limitation on soil respiratory activity following different levels of N fertilization in the field, and 2) the relationship between N-limited activities and growth under the assay conditions. Fertilization rate and the time since fertilization had significant impacts on the degree of N limitation of soil microbial activity. The highest fertilization rate showed the earliest and most persistent reduction in N limitation, as would be predicted from the higher concentration of extractable inorganic soil N observed with this treatment. Bacterial growth under the assay conditions, as estimated by quantitative-PCR of 16S rRNA genes, was less than twofold in soils demonstrating a rapid respiratory response (i.e. peak within 6-8 h of initiating incubation) to up to fourfold in soils demonstrating a slower respiratory response (i.e., peak response after 14 h of incubation). Increased respiratory response with N amendment was usually associated with increased cell growth, although for rapidly responding soils some C sources showed N-limited use without growth. This was likely due to exhaustion of the relatively low levels of available C amendment before growth was detected. The method appears useful for assessing N-limited microbial growth, and it may be effective as a rapid indicator of bioavailable soil N. It may also be a tool to evaluate the complexity of N limitation among various metabolic pathways found in soil microbial communities, particularly if linked to dynamics in community structure and gene activation.
Fil: Garland, J. L.. Dynamac Corporation; Estados Unidos
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Birmele, M.. Dynamac Corporation; Estados Unidos
Fil: Mackowiak, C. L.. University of Florida; Estados Unidos
Fil: Lehman, R. M.. North Central Agricultural Research Lab; Estados Unidos
Fil: Frey, S. D.. University Of New Hampshire; Estados Unidos
Materia
Community Level Physiological Profiling
Bioavailable N
N Limitation
Enrichment
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19868

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network_name_str CONICET Digital (CONICET)
spelling Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumptionGarland, J. L.Zabaloy, Maria CelinaBirmele, M.Mackowiak, C. L.Lehman, R. M.Frey, S. D.Community Level Physiological ProfilingBioavailable NN LimitationEnrichmenthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Nitrogen-limited soil microbial activity has important implications for soil carbon storage and nutrient availability, but previous methods for assessing resource limitation have been restricted, due to enrichment criteria (i.e., long incubation periods, high substrate amendments) and/or logistical constraints (e.g. use of radioisotopes). A microtiter-based assay of basal and substrate induced soil respiration based on O2 consumption may be a rapid, ecologically relevant means of assessing N limitation. The present study evaluated this approach by examining 1) the extent and duration of N limitation on soil respiratory activity following different levels of N fertilization in the field, and 2) the relationship between N-limited activities and growth under the assay conditions. Fertilization rate and the time since fertilization had significant impacts on the degree of N limitation of soil microbial activity. The highest fertilization rate showed the earliest and most persistent reduction in N limitation, as would be predicted from the higher concentration of extractable inorganic soil N observed with this treatment. Bacterial growth under the assay conditions, as estimated by quantitative-PCR of 16S rRNA genes, was less than twofold in soils demonstrating a rapid respiratory response (i.e. peak within 6-8 h of initiating incubation) to up to fourfold in soils demonstrating a slower respiratory response (i.e., peak response after 14 h of incubation). Increased respiratory response with N amendment was usually associated with increased cell growth, although for rapidly responding soils some C sources showed N-limited use without growth. This was likely due to exhaustion of the relatively low levels of available C amendment before growth was detected. The method appears useful for assessing N-limited microbial growth, and it may be effective as a rapid indicator of bioavailable soil N. It may also be a tool to evaluate the complexity of N limitation among various metabolic pathways found in soil microbial communities, particularly if linked to dynamics in community structure and gene activation.Fil: Garland, J. L.. Dynamac Corporation; Estados UnidosFil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaFil: Birmele, M.. Dynamac Corporation; Estados UnidosFil: Mackowiak, C. L.. University of Florida; Estados UnidosFil: Lehman, R. M.. North Central Agricultural Research Lab; Estados UnidosFil: Frey, S. D.. University Of New Hampshire; Estados UnidosElsevier2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19868Garland, J. L.; Zabaloy, Maria Celina; Birmele, M.; Mackowiak, C. L.; Lehman, R. M.; et al.; Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption; Elsevier; Soil Biology And Biochemistry; 47; 4-2012; 46-520038-0717CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0038071711004408info:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2011.12.016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:31Zoai:ri.conicet.gov.ar:11336/19868instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:31.942CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption
title Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption
spellingShingle Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption
Garland, J. L.
Community Level Physiological Profiling
Bioavailable N
N Limitation
Enrichment
title_short Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption
title_full Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption
title_fullStr Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption
title_full_unstemmed Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption
title_sort Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption
dc.creator.none.fl_str_mv Garland, J. L.
Zabaloy, Maria Celina
Birmele, M.
Mackowiak, C. L.
Lehman, R. M.
Frey, S. D.
author Garland, J. L.
author_facet Garland, J. L.
Zabaloy, Maria Celina
Birmele, M.
Mackowiak, C. L.
Lehman, R. M.
Frey, S. D.
author_role author
author2 Zabaloy, Maria Celina
Birmele, M.
Mackowiak, C. L.
Lehman, R. M.
Frey, S. D.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Community Level Physiological Profiling
Bioavailable N
N Limitation
Enrichment
topic Community Level Physiological Profiling
Bioavailable N
N Limitation
Enrichment
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Nitrogen-limited soil microbial activity has important implications for soil carbon storage and nutrient availability, but previous methods for assessing resource limitation have been restricted, due to enrichment criteria (i.e., long incubation periods, high substrate amendments) and/or logistical constraints (e.g. use of radioisotopes). A microtiter-based assay of basal and substrate induced soil respiration based on O2 consumption may be a rapid, ecologically relevant means of assessing N limitation. The present study evaluated this approach by examining 1) the extent and duration of N limitation on soil respiratory activity following different levels of N fertilization in the field, and 2) the relationship between N-limited activities and growth under the assay conditions. Fertilization rate and the time since fertilization had significant impacts on the degree of N limitation of soil microbial activity. The highest fertilization rate showed the earliest and most persistent reduction in N limitation, as would be predicted from the higher concentration of extractable inorganic soil N observed with this treatment. Bacterial growth under the assay conditions, as estimated by quantitative-PCR of 16S rRNA genes, was less than twofold in soils demonstrating a rapid respiratory response (i.e. peak within 6-8 h of initiating incubation) to up to fourfold in soils demonstrating a slower respiratory response (i.e., peak response after 14 h of incubation). Increased respiratory response with N amendment was usually associated with increased cell growth, although for rapidly responding soils some C sources showed N-limited use without growth. This was likely due to exhaustion of the relatively low levels of available C amendment before growth was detected. The method appears useful for assessing N-limited microbial growth, and it may be effective as a rapid indicator of bioavailable soil N. It may also be a tool to evaluate the complexity of N limitation among various metabolic pathways found in soil microbial communities, particularly if linked to dynamics in community structure and gene activation.
Fil: Garland, J. L.. Dynamac Corporation; Estados Unidos
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Birmele, M.. Dynamac Corporation; Estados Unidos
Fil: Mackowiak, C. L.. University of Florida; Estados Unidos
Fil: Lehman, R. M.. North Central Agricultural Research Lab; Estados Unidos
Fil: Frey, S. D.. University Of New Hampshire; Estados Unidos
description Nitrogen-limited soil microbial activity has important implications for soil carbon storage and nutrient availability, but previous methods for assessing resource limitation have been restricted, due to enrichment criteria (i.e., long incubation periods, high substrate amendments) and/or logistical constraints (e.g. use of radioisotopes). A microtiter-based assay of basal and substrate induced soil respiration based on O2 consumption may be a rapid, ecologically relevant means of assessing N limitation. The present study evaluated this approach by examining 1) the extent and duration of N limitation on soil respiratory activity following different levels of N fertilization in the field, and 2) the relationship between N-limited activities and growth under the assay conditions. Fertilization rate and the time since fertilization had significant impacts on the degree of N limitation of soil microbial activity. The highest fertilization rate showed the earliest and most persistent reduction in N limitation, as would be predicted from the higher concentration of extractable inorganic soil N observed with this treatment. Bacterial growth under the assay conditions, as estimated by quantitative-PCR of 16S rRNA genes, was less than twofold in soils demonstrating a rapid respiratory response (i.e. peak within 6-8 h of initiating incubation) to up to fourfold in soils demonstrating a slower respiratory response (i.e., peak response after 14 h of incubation). Increased respiratory response with N amendment was usually associated with increased cell growth, although for rapidly responding soils some C sources showed N-limited use without growth. This was likely due to exhaustion of the relatively low levels of available C amendment before growth was detected. The method appears useful for assessing N-limited microbial growth, and it may be effective as a rapid indicator of bioavailable soil N. It may also be a tool to evaluate the complexity of N limitation among various metabolic pathways found in soil microbial communities, particularly if linked to dynamics in community structure and gene activation.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19868
Garland, J. L.; Zabaloy, Maria Celina; Birmele, M.; Mackowiak, C. L.; Lehman, R. M.; et al.; Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption; Elsevier; Soil Biology And Biochemistry; 47; 4-2012; 46-52
0038-0717
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19868
identifier_str_mv Garland, J. L.; Zabaloy, Maria Celina; Birmele, M.; Mackowiak, C. L.; Lehman, R. M.; et al.; Examining N-limited soil microbial activity using community-level physiological profiling based on O2 consumption; Elsevier; Soil Biology And Biochemistry; 47; 4-2012; 46-52
0038-0717
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0038071711004408
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2011.12.016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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