Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling
- Autores
- Garland, J. L.; Mackowiak, C. L.; Zabaloy, Maria Celina
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Organic fertilizers provide long-term benefits to agronomic soils, but sometimes cause short-term reductions in crop yield due to microbially mediated nitrogen (N) immobilization. A simple, rapid method to assess the integrated use of both, carbon (C) and N by soil microbial communities will be a useful monitoring tool in production agriculture. The present study evaluated a new platform for performing community-level physiological profiles (CLPP) using fluorescent-based detection of O2 consumption by soil slurries within microtiter plates. Response of a spodic Florida soil to 3 organic fertilizer amendment treatments; (1) control with no organic amendment, (2) pelletized class A?A municipal biosolids amendment, and (3) fresh dairy waste solids amendment was measured in soils taken from a corn?rye crop rotation. The CLPP assay was used to assess endogenous and substrate induced (∼75 μg C as acetate, casein, coumaric acid, mannose, or asparagine g−1 soil) respiration, with and without assay N additions (8 μg N-NH4 g−1 soil). Endogenous and substrate-induced respiration were generally greater in the dairy waste-amended soils, as quantified by a reduced lag period and greater response peak. Stimulatory effects from biosolid-amended soils were less extensive and consistent. The degree of N limitation on microbial activity was determined by comparing the response peak with and without N amendment. This difference in response (Ndiff) was greatest for all treatments during the rye exponential growth phase (prior to heading), when extractable soil NH4-N and NO3-N concentrations were lowest (i.e., < 10 mg kg−1). The dairy waste treated soils had greater Ndiff values during the rye crop as compared to the other treatments, particularly for endogenous respiration and mannose-induced respiration. Ndiff was low in all treatments during the corn crop, where extractable soil NH4-N + NO3-N remained at or above 20 mg N kg−1. Plant yield data coincided with our estimates of N-limited microbial activity, with less mid-season rye biomass under dairy waste and no yield response with corn. Overall, these data indicate that this new method allows for a rapid, ecologically relevant evaluation of organic amendment impacts on microbial soil respiration and thereby plant yield response. Further characterization and interpretation of the variation in microbial respiration among specific C substrates and the relative impact of N amendments (i.e., Ndiff), will provide insight to C and N cycling in soils receiving organic N inputs.
Fil: Garland, J. L.. Dynamic Corporation. Kennedy Space Center; Estados Unidos
Fil: Mackowiak, C. L.. University Of Florida. Florida Museum Of History; Estados Unidos
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina - Materia
-
Soil Respiration
Community Level Physiological Profiling
N Limitation - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/16984
Ver los metadatos del registro completo
id |
CONICETDig_84b9ffc6e0d30c34a288c5ad007884c0 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/16984 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profilingGarland, J. L.Mackowiak, C. L.Zabaloy, Maria CelinaSoil RespirationCommunity Level Physiological ProfilingN Limitationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Organic fertilizers provide long-term benefits to agronomic soils, but sometimes cause short-term reductions in crop yield due to microbially mediated nitrogen (N) immobilization. A simple, rapid method to assess the integrated use of both, carbon (C) and N by soil microbial communities will be a useful monitoring tool in production agriculture. The present study evaluated a new platform for performing community-level physiological profiles (CLPP) using fluorescent-based detection of O2 consumption by soil slurries within microtiter plates. Response of a spodic Florida soil to 3 organic fertilizer amendment treatments; (1) control with no organic amendment, (2) pelletized class A?A municipal biosolids amendment, and (3) fresh dairy waste solids amendment was measured in soils taken from a corn?rye crop rotation. The CLPP assay was used to assess endogenous and substrate induced (∼75 μg C as acetate, casein, coumaric acid, mannose, or asparagine g−1 soil) respiration, with and without assay N additions (8 μg N-NH4 g−1 soil). Endogenous and substrate-induced respiration were generally greater in the dairy waste-amended soils, as quantified by a reduced lag period and greater response peak. Stimulatory effects from biosolid-amended soils were less extensive and consistent. The degree of N limitation on microbial activity was determined by comparing the response peak with and without N amendment. This difference in response (Ndiff) was greatest for all treatments during the rye exponential growth phase (prior to heading), when extractable soil NH4-N and NO3-N concentrations were lowest (i.e., < 10 mg kg−1). The dairy waste treated soils had greater Ndiff values during the rye crop as compared to the other treatments, particularly for endogenous respiration and mannose-induced respiration. Ndiff was low in all treatments during the corn crop, where extractable soil NH4-N + NO3-N remained at or above 20 mg N kg−1. Plant yield data coincided with our estimates of N-limited microbial activity, with less mid-season rye biomass under dairy waste and no yield response with corn. Overall, these data indicate that this new method allows for a rapid, ecologically relevant evaluation of organic amendment impacts on microbial soil respiration and thereby plant yield response. Further characterization and interpretation of the variation in microbial respiration among specific C substrates and the relative impact of N amendments (i.e., Ndiff), will provide insight to C and N cycling in soils receiving organic N inputs.Fil: Garland, J. L.. Dynamic Corporation. Kennedy Space Center; Estados UnidosFil: Mackowiak, C. L.. University Of Florida. Florida Museum Of History; Estados UnidosFil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaElsevier Science2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16984Garland, J. L.; Mackowiak, C. L.; Zabaloy, Maria Celina; Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling; Elsevier Science; Applied Soil Ecology; 44; 3; 3-2010; 262-2690929-1393enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139310000089info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2010.01.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:21Zoai:ri.conicet.gov.ar:11336/16984instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:22.27CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling |
title |
Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling |
spellingShingle |
Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling Garland, J. L. Soil Respiration Community Level Physiological Profiling N Limitation |
title_short |
Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling |
title_full |
Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling |
title_fullStr |
Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling |
title_full_unstemmed |
Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling |
title_sort |
Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling |
dc.creator.none.fl_str_mv |
Garland, J. L. Mackowiak, C. L. Zabaloy, Maria Celina |
author |
Garland, J. L. |
author_facet |
Garland, J. L. Mackowiak, C. L. Zabaloy, Maria Celina |
author_role |
author |
author2 |
Mackowiak, C. L. Zabaloy, Maria Celina |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Soil Respiration Community Level Physiological Profiling N Limitation |
topic |
Soil Respiration Community Level Physiological Profiling N Limitation |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/4.1 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Organic fertilizers provide long-term benefits to agronomic soils, but sometimes cause short-term reductions in crop yield due to microbially mediated nitrogen (N) immobilization. A simple, rapid method to assess the integrated use of both, carbon (C) and N by soil microbial communities will be a useful monitoring tool in production agriculture. The present study evaluated a new platform for performing community-level physiological profiles (CLPP) using fluorescent-based detection of O2 consumption by soil slurries within microtiter plates. Response of a spodic Florida soil to 3 organic fertilizer amendment treatments; (1) control with no organic amendment, (2) pelletized class A?A municipal biosolids amendment, and (3) fresh dairy waste solids amendment was measured in soils taken from a corn?rye crop rotation. The CLPP assay was used to assess endogenous and substrate induced (∼75 μg C as acetate, casein, coumaric acid, mannose, or asparagine g−1 soil) respiration, with and without assay N additions (8 μg N-NH4 g−1 soil). Endogenous and substrate-induced respiration were generally greater in the dairy waste-amended soils, as quantified by a reduced lag period and greater response peak. Stimulatory effects from biosolid-amended soils were less extensive and consistent. The degree of N limitation on microbial activity was determined by comparing the response peak with and without N amendment. This difference in response (Ndiff) was greatest for all treatments during the rye exponential growth phase (prior to heading), when extractable soil NH4-N and NO3-N concentrations were lowest (i.e., < 10 mg kg−1). The dairy waste treated soils had greater Ndiff values during the rye crop as compared to the other treatments, particularly for endogenous respiration and mannose-induced respiration. Ndiff was low in all treatments during the corn crop, where extractable soil NH4-N + NO3-N remained at or above 20 mg N kg−1. Plant yield data coincided with our estimates of N-limited microbial activity, with less mid-season rye biomass under dairy waste and no yield response with corn. Overall, these data indicate that this new method allows for a rapid, ecologically relevant evaluation of organic amendment impacts on microbial soil respiration and thereby plant yield response. Further characterization and interpretation of the variation in microbial respiration among specific C substrates and the relative impact of N amendments (i.e., Ndiff), will provide insight to C and N cycling in soils receiving organic N inputs. Fil: Garland, J. L.. Dynamic Corporation. Kennedy Space Center; Estados Unidos Fil: Mackowiak, C. L.. University Of Florida. Florida Museum Of History; Estados Unidos Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina |
description |
Organic fertilizers provide long-term benefits to agronomic soils, but sometimes cause short-term reductions in crop yield due to microbially mediated nitrogen (N) immobilization. A simple, rapid method to assess the integrated use of both, carbon (C) and N by soil microbial communities will be a useful monitoring tool in production agriculture. The present study evaluated a new platform for performing community-level physiological profiles (CLPP) using fluorescent-based detection of O2 consumption by soil slurries within microtiter plates. Response of a spodic Florida soil to 3 organic fertilizer amendment treatments; (1) control with no organic amendment, (2) pelletized class A?A municipal biosolids amendment, and (3) fresh dairy waste solids amendment was measured in soils taken from a corn?rye crop rotation. The CLPP assay was used to assess endogenous and substrate induced (∼75 μg C as acetate, casein, coumaric acid, mannose, or asparagine g−1 soil) respiration, with and without assay N additions (8 μg N-NH4 g−1 soil). Endogenous and substrate-induced respiration were generally greater in the dairy waste-amended soils, as quantified by a reduced lag period and greater response peak. Stimulatory effects from biosolid-amended soils were less extensive and consistent. The degree of N limitation on microbial activity was determined by comparing the response peak with and without N amendment. This difference in response (Ndiff) was greatest for all treatments during the rye exponential growth phase (prior to heading), when extractable soil NH4-N and NO3-N concentrations were lowest (i.e., < 10 mg kg−1). The dairy waste treated soils had greater Ndiff values during the rye crop as compared to the other treatments, particularly for endogenous respiration and mannose-induced respiration. Ndiff was low in all treatments during the corn crop, where extractable soil NH4-N + NO3-N remained at or above 20 mg N kg−1. Plant yield data coincided with our estimates of N-limited microbial activity, with less mid-season rye biomass under dairy waste and no yield response with corn. Overall, these data indicate that this new method allows for a rapid, ecologically relevant evaluation of organic amendment impacts on microbial soil respiration and thereby plant yield response. Further characterization and interpretation of the variation in microbial respiration among specific C substrates and the relative impact of N amendments (i.e., Ndiff), will provide insight to C and N cycling in soils receiving organic N inputs. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/16984 Garland, J. L.; Mackowiak, C. L.; Zabaloy, Maria Celina; Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling; Elsevier Science; Applied Soil Ecology; 44; 3; 3-2010; 262-269 0929-1393 |
url |
http://hdl.handle.net/11336/16984 |
identifier_str_mv |
Garland, J. L.; Mackowiak, C. L.; Zabaloy, Maria Celina; Organic waste amendment effects on soil microbial activity in a corn-rye rotation: Application of a new approach to community-level physiological profiling; Elsevier Science; Applied Soil Ecology; 44; 3; 3-2010; 262-269 0929-1393 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139310000089 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2010.01.003 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614393863602176 |
score |
13.070432 |