Assessment of microbial community function and structure in soil microcosms exposed to glyphosate
- Autores
- Zabaloy, Maria Celina; Gomez, Elena del Valle; Garland, Jay L.; Gomez, Marisa Anahi
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The large scale use of glyphosate to control weeds in transgenic crops and in no-till management systems emphasizes the need to understand its effects on soil microbial communities. The herbicide may change the soil environment due to toxicity to soil microorganisms and through the influx of carbon (C), phosphorus (P) and nitrogen (N) from the cometabolic decay of glyphosate. This study evaluated both the potential effects of glyphosate treatments on microbial community structure and function in laboratory incubation of soils. Soil from two sites in the Pampa region of Argentina (Vertic Argiudoll from Zavalla, ZAV; Petrocalcic Paleustoll from Coronel Dorrego, DOR), with long exposure to glyphosate were used in soil microcosms amended with different doses of herbicide (0, 15 and 150mgkg -1) and incubated for 7 days. Soil from a natural grassland at the ZAV site was used as a reference soil. Community respiration in response to different C and nutrient (N and P) sources, including glyphosate, were assessed using an O 2 consumption-based assay in microtiter plates. Microbial community structure was analyzed using quantitative PCR (qPCR) to estimate the bacterial abundance and terminal restriction fragment length polymorphism (T-RFLP) to investigate the structure of the bacterial community. Glyphosate addition to the microcosms had minimal effects on both structural and functional measures of the microbial community. The addition of a high dose of glyphosate to soil microcosms from one agricultural site significantly reduced N limitation, suggesting that glyphosate breakdown provided N for microbial activity. However, the immediate respiratory response of the reference and chronically exposed soils to glyphosate was distinctive. Glyphosate increased respiration in the reference grassland soil, potentially due to a stress response of glyphosate sensitive species, while it depressed respiration in the chronically exposed soil, probably as a result of selection for organisms acclimated for rapid assimilation of substrates from the cometabolic decay of the molecule. These results suggest that longer term studies involving repeated addition of glyphosate to previously unexposed soils are needed to understand important shifts in community metabolism caused by the typical agricultural use of this herbicide.
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Gomez, Elena del Valle. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
Fil: Garland, Jay L.. United States Environmental Protection Agency; Estados Unidos. Kennedy Space Center. Dynamac Corporation; Estados Unidos
Fil: Gomez, Marisa Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina - Materia
-
Community-Level Physiological Profiling
Glyphosate Toxicity
Quantitative Pcr
Soil Respiration
T-Rflp - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/76596
Ver los metadatos del registro completo
id |
CONICETDig_21fdda877721a22c46955cbc524e18c4 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/76596 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Assessment of microbial community function and structure in soil microcosms exposed to glyphosateZabaloy, Maria CelinaGomez, Elena del ValleGarland, Jay L.Gomez, Marisa AnahiCommunity-Level Physiological ProfilingGlyphosate ToxicityQuantitative PcrSoil RespirationT-Rflphttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The large scale use of glyphosate to control weeds in transgenic crops and in no-till management systems emphasizes the need to understand its effects on soil microbial communities. The herbicide may change the soil environment due to toxicity to soil microorganisms and through the influx of carbon (C), phosphorus (P) and nitrogen (N) from the cometabolic decay of glyphosate. This study evaluated both the potential effects of glyphosate treatments on microbial community structure and function in laboratory incubation of soils. Soil from two sites in the Pampa region of Argentina (Vertic Argiudoll from Zavalla, ZAV; Petrocalcic Paleustoll from Coronel Dorrego, DOR), with long exposure to glyphosate were used in soil microcosms amended with different doses of herbicide (0, 15 and 150mgkg -1) and incubated for 7 days. Soil from a natural grassland at the ZAV site was used as a reference soil. Community respiration in response to different C and nutrient (N and P) sources, including glyphosate, were assessed using an O 2 consumption-based assay in microtiter plates. Microbial community structure was analyzed using quantitative PCR (qPCR) to estimate the bacterial abundance and terminal restriction fragment length polymorphism (T-RFLP) to investigate the structure of the bacterial community. Glyphosate addition to the microcosms had minimal effects on both structural and functional measures of the microbial community. The addition of a high dose of glyphosate to soil microcosms from one agricultural site significantly reduced N limitation, suggesting that glyphosate breakdown provided N for microbial activity. However, the immediate respiratory response of the reference and chronically exposed soils to glyphosate was distinctive. Glyphosate increased respiration in the reference grassland soil, potentially due to a stress response of glyphosate sensitive species, while it depressed respiration in the chronically exposed soil, probably as a result of selection for organisms acclimated for rapid assimilation of substrates from the cometabolic decay of the molecule. These results suggest that longer term studies involving repeated addition of glyphosate to previously unexposed soils are needed to understand important shifts in community metabolism caused by the typical agricultural use of this herbicide.Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Gomez, Elena del Valle. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; ArgentinaFil: Garland, Jay L.. United States Environmental Protection Agency; Estados Unidos. Kennedy Space Center. Dynamac Corporation; Estados UnidosFil: Gomez, Marisa Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaElsevier Science2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76596Zabaloy, Maria Celina; Gomez, Elena del Valle; Garland, Jay L.; Gomez, Marisa Anahi; Assessment of microbial community function and structure in soil microcosms exposed to glyphosate; Elsevier Science; Applied Soil Ecology; 61; 10-2012; 333-3390929-1393CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139311003258info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2011.12.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:11Zoai:ri.conicet.gov.ar:11336/76596instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:11.441CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Assessment of microbial community function and structure in soil microcosms exposed to glyphosate |
title |
Assessment of microbial community function and structure in soil microcosms exposed to glyphosate |
spellingShingle |
Assessment of microbial community function and structure in soil microcosms exposed to glyphosate Zabaloy, Maria Celina Community-Level Physiological Profiling Glyphosate Toxicity Quantitative Pcr Soil Respiration T-Rflp |
title_short |
Assessment of microbial community function and structure in soil microcosms exposed to glyphosate |
title_full |
Assessment of microbial community function and structure in soil microcosms exposed to glyphosate |
title_fullStr |
Assessment of microbial community function and structure in soil microcosms exposed to glyphosate |
title_full_unstemmed |
Assessment of microbial community function and structure in soil microcosms exposed to glyphosate |
title_sort |
Assessment of microbial community function and structure in soil microcosms exposed to glyphosate |
dc.creator.none.fl_str_mv |
Zabaloy, Maria Celina Gomez, Elena del Valle Garland, Jay L. Gomez, Marisa Anahi |
author |
Zabaloy, Maria Celina |
author_facet |
Zabaloy, Maria Celina Gomez, Elena del Valle Garland, Jay L. Gomez, Marisa Anahi |
author_role |
author |
author2 |
Gomez, Elena del Valle Garland, Jay L. Gomez, Marisa Anahi |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Community-Level Physiological Profiling Glyphosate Toxicity Quantitative Pcr Soil Respiration T-Rflp |
topic |
Community-Level Physiological Profiling Glyphosate Toxicity Quantitative Pcr Soil Respiration T-Rflp |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.1 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The large scale use of glyphosate to control weeds in transgenic crops and in no-till management systems emphasizes the need to understand its effects on soil microbial communities. The herbicide may change the soil environment due to toxicity to soil microorganisms and through the influx of carbon (C), phosphorus (P) and nitrogen (N) from the cometabolic decay of glyphosate. This study evaluated both the potential effects of glyphosate treatments on microbial community structure and function in laboratory incubation of soils. Soil from two sites in the Pampa region of Argentina (Vertic Argiudoll from Zavalla, ZAV; Petrocalcic Paleustoll from Coronel Dorrego, DOR), with long exposure to glyphosate were used in soil microcosms amended with different doses of herbicide (0, 15 and 150mgkg -1) and incubated for 7 days. Soil from a natural grassland at the ZAV site was used as a reference soil. Community respiration in response to different C and nutrient (N and P) sources, including glyphosate, were assessed using an O 2 consumption-based assay in microtiter plates. Microbial community structure was analyzed using quantitative PCR (qPCR) to estimate the bacterial abundance and terminal restriction fragment length polymorphism (T-RFLP) to investigate the structure of the bacterial community. Glyphosate addition to the microcosms had minimal effects on both structural and functional measures of the microbial community. The addition of a high dose of glyphosate to soil microcosms from one agricultural site significantly reduced N limitation, suggesting that glyphosate breakdown provided N for microbial activity. However, the immediate respiratory response of the reference and chronically exposed soils to glyphosate was distinctive. Glyphosate increased respiration in the reference grassland soil, potentially due to a stress response of glyphosate sensitive species, while it depressed respiration in the chronically exposed soil, probably as a result of selection for organisms acclimated for rapid assimilation of substrates from the cometabolic decay of the molecule. These results suggest that longer term studies involving repeated addition of glyphosate to previously unexposed soils are needed to understand important shifts in community metabolism caused by the typical agricultural use of this herbicide. Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Gomez, Elena del Valle. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina Fil: Garland, Jay L.. United States Environmental Protection Agency; Estados Unidos. Kennedy Space Center. Dynamac Corporation; Estados Unidos Fil: Gomez, Marisa Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina |
description |
The large scale use of glyphosate to control weeds in transgenic crops and in no-till management systems emphasizes the need to understand its effects on soil microbial communities. The herbicide may change the soil environment due to toxicity to soil microorganisms and through the influx of carbon (C), phosphorus (P) and nitrogen (N) from the cometabolic decay of glyphosate. This study evaluated both the potential effects of glyphosate treatments on microbial community structure and function in laboratory incubation of soils. Soil from two sites in the Pampa region of Argentina (Vertic Argiudoll from Zavalla, ZAV; Petrocalcic Paleustoll from Coronel Dorrego, DOR), with long exposure to glyphosate were used in soil microcosms amended with different doses of herbicide (0, 15 and 150mgkg -1) and incubated for 7 days. Soil from a natural grassland at the ZAV site was used as a reference soil. Community respiration in response to different C and nutrient (N and P) sources, including glyphosate, were assessed using an O 2 consumption-based assay in microtiter plates. Microbial community structure was analyzed using quantitative PCR (qPCR) to estimate the bacterial abundance and terminal restriction fragment length polymorphism (T-RFLP) to investigate the structure of the bacterial community. Glyphosate addition to the microcosms had minimal effects on both structural and functional measures of the microbial community. The addition of a high dose of glyphosate to soil microcosms from one agricultural site significantly reduced N limitation, suggesting that glyphosate breakdown provided N for microbial activity. However, the immediate respiratory response of the reference and chronically exposed soils to glyphosate was distinctive. Glyphosate increased respiration in the reference grassland soil, potentially due to a stress response of glyphosate sensitive species, while it depressed respiration in the chronically exposed soil, probably as a result of selection for organisms acclimated for rapid assimilation of substrates from the cometabolic decay of the molecule. These results suggest that longer term studies involving repeated addition of glyphosate to previously unexposed soils are needed to understand important shifts in community metabolism caused by the typical agricultural use of this herbicide. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/76596 Zabaloy, Maria Celina; Gomez, Elena del Valle; Garland, Jay L.; Gomez, Marisa Anahi; Assessment of microbial community function and structure in soil microcosms exposed to glyphosate; Elsevier Science; Applied Soil Ecology; 61; 10-2012; 333-339 0929-1393 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/76596 |
identifier_str_mv |
Zabaloy, Maria Celina; Gomez, Elena del Valle; Garland, Jay L.; Gomez, Marisa Anahi; Assessment of microbial community function and structure in soil microcosms exposed to glyphosate; Elsevier Science; Applied Soil Ecology; 61; 10-2012; 333-339 0929-1393 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139311003258 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2011.12.004 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613470843043840 |
score |
13.070432 |