Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate

Autores
Kuc, Vanesa; Vázquez, Susana Claudia; Hernández, Edgardo; Martinez Alvarez, Lucas Manuel; Villalba Primitz, Julia Elena Rosina; Mac Cormack, Walter Patricio; Ruberto, Lucas Adolfo Mauro
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hydrocarboncontamination in soils from extremely cold areas, such as those from Antarctica,requires the development of specifc remediation strategies for cleaning up anthropogenicpollution. Previous reports evidenced that after on-site biostimulation processof gasoil-contaminated Antarctic soils, 20% of the initial hydrocarbons remainedundegraded (mainly C11?C14 n-alkanes). In the present work, these n-alkanes wereadded as sole carbon and energy source to enrichment cultures inoculated with thepreviously treated soil (biostimulation) as microorganism?s source to investigatechanges occurring in the bacterial community structure. Three subcultures (8, 16,and 24fdays) were performed from each enrichment culture. Changes in bacterial communitiesamong dilerent cultures and its subcultures were evidenced by Denaturing GradientGel Electrophoresis (DGGE). Results showed that even dilerences of one C in thealkane chain-length led to dilerent community structures that evolved divergentlyfrom the original one. Clusters analysis showed that while samples grouped mainlyby culture time, substrate-dependent dilerences were also evident. Isolation ofbiological tools for bioremediation from the cultures showed that Pseudomonadaceaemembers were omnipresent, whereas Rhodococcus spp. were obtained in cultures withthe longest chain-length substrates. Results provided evidence about the presenceof certain substrate preference of soil bacteria (even when substrates dilered onlyin one C-atom of their chain-length), leading to dilerent community structures.A collection of psychrotolerant hydrocarbon degrading/tolerant strains was obtained,representing a valuable tool for the design of a bioaugmentation strategy as a second,more specifc stage, targeting the remnant hydrocarbons after a frst bioremediationprocess involving biostimulation.
Fil: Kuc, Vanesa. Universidad Nacional de Quilmes; Argentina
Fil: Vázquez, Susana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Hernández, Edgardo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Martinez Alvarez, Lucas Manuel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Villalba Primitz, Julia Elena Rosina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Mac Cormack, Walter Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Materia
BIOREMEDIATION
ENRICHMENT CULTURES
n-ALKANES
ANTARCTICA
BACTERIAL COMMUNITY STRUCTURE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121458
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrateKuc, VanesaVázquez, Susana ClaudiaHernández, EdgardoMartinez Alvarez, Lucas ManuelVillalba Primitz, Julia Elena RosinaMac Cormack, Walter PatricioRuberto, Lucas Adolfo MauroBIOREMEDIATIONENRICHMENT CULTURESn-ALKANESANTARCTICABACTERIAL COMMUNITY STRUCTUREhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hydrocarboncontamination in soils from extremely cold areas, such as those from Antarctica,requires the development of specifc remediation strategies for cleaning up anthropogenicpollution. Previous reports evidenced that after on-site biostimulation processof gasoil-contaminated Antarctic soils, 20% of the initial hydrocarbons remainedundegraded (mainly C11?C14 n-alkanes). In the present work, these n-alkanes wereadded as sole carbon and energy source to enrichment cultures inoculated with thepreviously treated soil (biostimulation) as microorganism?s source to investigatechanges occurring in the bacterial community structure. Three subcultures (8, 16,and 24fdays) were performed from each enrichment culture. Changes in bacterial communitiesamong dilerent cultures and its subcultures were evidenced by Denaturing GradientGel Electrophoresis (DGGE). Results showed that even dilerences of one C in thealkane chain-length led to dilerent community structures that evolved divergentlyfrom the original one. Clusters analysis showed that while samples grouped mainlyby culture time, substrate-dependent dilerences were also evident. Isolation ofbiological tools for bioremediation from the cultures showed that Pseudomonadaceaemembers were omnipresent, whereas Rhodococcus spp. were obtained in cultures withthe longest chain-length substrates. Results provided evidence about the presenceof certain substrate preference of soil bacteria (even when substrates dilered onlyin one C-atom of their chain-length), leading to dilerent community structures.A collection of psychrotolerant hydrocarbon degrading/tolerant strains was obtained,representing a valuable tool for the design of a bioaugmentation strategy as a second,more specifc stage, targeting the remnant hydrocarbons after a frst bioremediationprocess involving biostimulation.Fil: Kuc, Vanesa. Universidad Nacional de Quilmes; ArgentinaFil: Vázquez, Susana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Hernández, Edgardo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: Martinez Alvarez, Lucas Manuel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Villalba Primitz, Julia Elena Rosina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; ArgentinaFil: Mac Cormack, Walter Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaSpringer2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121458Kuc, Vanesa; Vázquez, Susana Claudia; Hernández, Edgardo; Martinez Alvarez, Lucas Manuel; Villalba Primitz, Julia Elena Rosina; et al.; Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate; Springer; Polar Biology; 42; 6; 5-2019; 1157-11660722-40601432-2056CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00300-019-02508-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s00300-019-02508-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:52Zoai:ri.conicet.gov.ar:11336/121458instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:52.858CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate
title Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate
spellingShingle Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate
Kuc, Vanesa
BIOREMEDIATION
ENRICHMENT CULTURES
n-ALKANES
ANTARCTICA
BACTERIAL COMMUNITY STRUCTURE
title_short Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate
title_full Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate
title_fullStr Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate
title_full_unstemmed Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate
title_sort Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate
dc.creator.none.fl_str_mv Kuc, Vanesa
Vázquez, Susana Claudia
Hernández, Edgardo
Martinez Alvarez, Lucas Manuel
Villalba Primitz, Julia Elena Rosina
Mac Cormack, Walter Patricio
Ruberto, Lucas Adolfo Mauro
author Kuc, Vanesa
author_facet Kuc, Vanesa
Vázquez, Susana Claudia
Hernández, Edgardo
Martinez Alvarez, Lucas Manuel
Villalba Primitz, Julia Elena Rosina
Mac Cormack, Walter Patricio
Ruberto, Lucas Adolfo Mauro
author_role author
author2 Vázquez, Susana Claudia
Hernández, Edgardo
Martinez Alvarez, Lucas Manuel
Villalba Primitz, Julia Elena Rosina
Mac Cormack, Walter Patricio
Ruberto, Lucas Adolfo Mauro
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOREMEDIATION
ENRICHMENT CULTURES
n-ALKANES
ANTARCTICA
BACTERIAL COMMUNITY STRUCTURE
topic BIOREMEDIATION
ENRICHMENT CULTURES
n-ALKANES
ANTARCTICA
BACTERIAL COMMUNITY STRUCTURE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hydrocarboncontamination in soils from extremely cold areas, such as those from Antarctica,requires the development of specifc remediation strategies for cleaning up anthropogenicpollution. Previous reports evidenced that after on-site biostimulation processof gasoil-contaminated Antarctic soils, 20% of the initial hydrocarbons remainedundegraded (mainly C11?C14 n-alkanes). In the present work, these n-alkanes wereadded as sole carbon and energy source to enrichment cultures inoculated with thepreviously treated soil (biostimulation) as microorganism?s source to investigatechanges occurring in the bacterial community structure. Three subcultures (8, 16,and 24fdays) were performed from each enrichment culture. Changes in bacterial communitiesamong dilerent cultures and its subcultures were evidenced by Denaturing GradientGel Electrophoresis (DGGE). Results showed that even dilerences of one C in thealkane chain-length led to dilerent community structures that evolved divergentlyfrom the original one. Clusters analysis showed that while samples grouped mainlyby culture time, substrate-dependent dilerences were also evident. Isolation ofbiological tools for bioremediation from the cultures showed that Pseudomonadaceaemembers were omnipresent, whereas Rhodococcus spp. were obtained in cultures withthe longest chain-length substrates. Results provided evidence about the presenceof certain substrate preference of soil bacteria (even when substrates dilered onlyin one C-atom of their chain-length), leading to dilerent community structures.A collection of psychrotolerant hydrocarbon degrading/tolerant strains was obtained,representing a valuable tool for the design of a bioaugmentation strategy as a second,more specifc stage, targeting the remnant hydrocarbons after a frst bioremediationprocess involving biostimulation.
Fil: Kuc, Vanesa. Universidad Nacional de Quilmes; Argentina
Fil: Vázquez, Susana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Hernández, Edgardo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Martinez Alvarez, Lucas Manuel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Villalba Primitz, Julia Elena Rosina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Mac Cormack, Walter Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
description Hydrocarboncontamination in soils from extremely cold areas, such as those from Antarctica,requires the development of specifc remediation strategies for cleaning up anthropogenicpollution. Previous reports evidenced that after on-site biostimulation processof gasoil-contaminated Antarctic soils, 20% of the initial hydrocarbons remainedundegraded (mainly C11?C14 n-alkanes). In the present work, these n-alkanes wereadded as sole carbon and energy source to enrichment cultures inoculated with thepreviously treated soil (biostimulation) as microorganism?s source to investigatechanges occurring in the bacterial community structure. Three subcultures (8, 16,and 24fdays) were performed from each enrichment culture. Changes in bacterial communitiesamong dilerent cultures and its subcultures were evidenced by Denaturing GradientGel Electrophoresis (DGGE). Results showed that even dilerences of one C in thealkane chain-length led to dilerent community structures that evolved divergentlyfrom the original one. Clusters analysis showed that while samples grouped mainlyby culture time, substrate-dependent dilerences were also evident. Isolation ofbiological tools for bioremediation from the cultures showed that Pseudomonadaceaemembers were omnipresent, whereas Rhodococcus spp. were obtained in cultures withthe longest chain-length substrates. Results provided evidence about the presenceof certain substrate preference of soil bacteria (even when substrates dilered onlyin one C-atom of their chain-length), leading to dilerent community structures.A collection of psychrotolerant hydrocarbon degrading/tolerant strains was obtained,representing a valuable tool for the design of a bioaugmentation strategy as a second,more specifc stage, targeting the remnant hydrocarbons after a frst bioremediationprocess involving biostimulation.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121458
Kuc, Vanesa; Vázquez, Susana Claudia; Hernández, Edgardo; Martinez Alvarez, Lucas Manuel; Villalba Primitz, Julia Elena Rosina; et al.; Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate; Springer; Polar Biology; 42; 6; 5-2019; 1157-1166
0722-4060
1432-2056
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121458
identifier_str_mv Kuc, Vanesa; Vázquez, Susana Claudia; Hernández, Edgardo; Martinez Alvarez, Lucas Manuel; Villalba Primitz, Julia Elena Rosina; et al.; Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate; Springer; Polar Biology; 42; 6; 5-2019; 1157-1166
0722-4060
1432-2056
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1007/s00300-019-02508-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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