Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri

Autores
Luján, Enrique Ernesto; Marcinkevicius, Karenina; D'almeida, Romina Elisa; Fogliata, G.; Arena, Mario Eduardo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Nomuraea rileyi ARSEF 4094, an entomopathogenic fungus (EF) isolated from Spodoptera frugiperda, produces secondary metabolites (SM) that interfere with the development of the insects that attack and bacteria from the insect body. It is known that the addition of insects into the fungal growth medium stimulates the production of SM. SM are known to affect bacterial growth in their planktonic and biofilm (BF) states. Therefore, the aim of this study was to evaluate the inhibitory capacity of N. rileyi in the presence of S. frugiperda on Xanthomonas citri subsp. citri (Xcc) growth and BF formation. This bacterium causes citrus canker, the main crop in Tucumán.EF was cultured in SMY medium with S. frugiperda and incubated at 28 °C during 15 days, at 180 rpm. Fungal and insect controls with the same treatment were made. Ethyl acetate extracts from biomass and supernatant from each condition were obtained. Xcc growth inhibition was determined by the microdilution method at 48 h and the inhibition of BF formation was quantified by a colorimetric method.The extract from fungal-insect biomass was the most efficient in inhibiting BF formation (38%), affecting Xcc growth in only 15%. While the extract from fungal biomass inhibited the growth of the bacteria in 32% and only 9% the formation of BF. According to this, the presence of insects could stimulate the production of SM, which interfere with the synthesis of BF.The results showed the potential use of extracts from N. rileyi as a natural strategy and environmentally acceptable for crop protection against diseases caused by pathogenic microorganisms.
Fil: Luján, Enrique Ernesto. Universidad Nacional de Tucumán; Argentina
Fil: Marcinkevicius, Karenina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: D'almeida, Romina Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Fogliata, G.. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (P); Argentina
Fil: Arena, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
XXXIII Jornadas Científicas de la Asociación de Biología de Tucumán
Tafi del Valle
Argentina
Asociación de Biología de Tucumán
Materia
NOMURAEA RILEYI
ENTOMOPATHOGENIC FUNGI
BIOFILM
XANTHOMONAS CITRI SUBSP CITRI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237048
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/237048
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citriLuján, Enrique ErnestoMarcinkevicius, KareninaD'almeida, Romina ElisaFogliata, G.Arena, Mario EduardoNOMURAEA RILEYIENTOMOPATHOGENIC FUNGIBIOFILMXANTHOMONAS CITRI SUBSP CITRIhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Nomuraea rileyi ARSEF 4094, an entomopathogenic fungus (EF) isolated from Spodoptera frugiperda, produces secondary metabolites (SM) that interfere with the development of the insects that attack and bacteria from the insect body. It is known that the addition of insects into the fungal growth medium stimulates the production of SM. SM are known to affect bacterial growth in their planktonic and biofilm (BF) states. Therefore, the aim of this study was to evaluate the inhibitory capacity of N. rileyi in the presence of S. frugiperda on Xanthomonas citri subsp. citri (Xcc) growth and BF formation. This bacterium causes citrus canker, the main crop in Tucumán.EF was cultured in SMY medium with S. frugiperda and incubated at 28 °C during 15 days, at 180 rpm. Fungal and insect controls with the same treatment were made. Ethyl acetate extracts from biomass and supernatant from each condition were obtained. Xcc growth inhibition was determined by the microdilution method at 48 h and the inhibition of BF formation was quantified by a colorimetric method.The extract from fungal-insect biomass was the most efficient in inhibiting BF formation (38%), affecting Xcc growth in only 15%. While the extract from fungal biomass inhibited the growth of the bacteria in 32% and only 9% the formation of BF. According to this, the presence of insects could stimulate the production of SM, which interfere with the synthesis of BF.The results showed the potential use of extracts from N. rileyi as a natural strategy and environmentally acceptable for crop protection against diseases caused by pathogenic microorganisms.Fil: Luján, Enrique Ernesto. Universidad Nacional de Tucumán; ArgentinaFil: Marcinkevicius, Karenina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: D'almeida, Romina Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Fogliata, G.. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (P); ArgentinaFil: Arena, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaXXXIII Jornadas Científicas de la Asociación de Biología de TucumánTafi del ValleArgentinaAsociación de Biología de TucumánTech Science Press2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237048Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri; XXXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafi del Valle; Argentina; 2016; 17-170327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://asobioltuc.com/uploads/archivos/1503947552_QmlvY2VsbCBYWFhJSUkgSkMgMjAxNi5wZGY=.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:41Zoai:ri.conicet.gov.ar:11336/237048instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:41.547CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri
title Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri
spellingShingle Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri
Luján, Enrique Ernesto
NOMURAEA RILEYI
ENTOMOPATHOGENIC FUNGI
BIOFILM
XANTHOMONAS CITRI SUBSP CITRI
title_short Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri
title_full Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri
title_fullStr Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri
title_full_unstemmed Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri
title_sort Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri
dc.creator.none.fl_str_mv Luján, Enrique Ernesto
Marcinkevicius, Karenina
D'almeida, Romina Elisa
Fogliata, G.
Arena, Mario Eduardo
author Luján, Enrique Ernesto
author_facet Luján, Enrique Ernesto
Marcinkevicius, Karenina
D'almeida, Romina Elisa
Fogliata, G.
Arena, Mario Eduardo
author_role author
author2 Marcinkevicius, Karenina
D'almeida, Romina Elisa
Fogliata, G.
Arena, Mario Eduardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv NOMURAEA RILEYI
ENTOMOPATHOGENIC FUNGI
BIOFILM
XANTHOMONAS CITRI SUBSP CITRI
topic NOMURAEA RILEYI
ENTOMOPATHOGENIC FUNGI
BIOFILM
XANTHOMONAS CITRI SUBSP CITRI
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Nomuraea rileyi ARSEF 4094, an entomopathogenic fungus (EF) isolated from Spodoptera frugiperda, produces secondary metabolites (SM) that interfere with the development of the insects that attack and bacteria from the insect body. It is known that the addition of insects into the fungal growth medium stimulates the production of SM. SM are known to affect bacterial growth in their planktonic and biofilm (BF) states. Therefore, the aim of this study was to evaluate the inhibitory capacity of N. rileyi in the presence of S. frugiperda on Xanthomonas citri subsp. citri (Xcc) growth and BF formation. This bacterium causes citrus canker, the main crop in Tucumán.EF was cultured in SMY medium with S. frugiperda and incubated at 28 °C during 15 days, at 180 rpm. Fungal and insect controls with the same treatment were made. Ethyl acetate extracts from biomass and supernatant from each condition were obtained. Xcc growth inhibition was determined by the microdilution method at 48 h and the inhibition of BF formation was quantified by a colorimetric method.The extract from fungal-insect biomass was the most efficient in inhibiting BF formation (38%), affecting Xcc growth in only 15%. While the extract from fungal biomass inhibited the growth of the bacteria in 32% and only 9% the formation of BF. According to this, the presence of insects could stimulate the production of SM, which interfere with the synthesis of BF.The results showed the potential use of extracts from N. rileyi as a natural strategy and environmentally acceptable for crop protection against diseases caused by pathogenic microorganisms.
Fil: Luján, Enrique Ernesto. Universidad Nacional de Tucumán; Argentina
Fil: Marcinkevicius, Karenina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: D'almeida, Romina Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Fogliata, G.. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (P); Argentina
Fil: Arena, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
XXXIII Jornadas Científicas de la Asociación de Biología de Tucumán
Tafi del Valle
Argentina
Asociación de Biología de Tucumán
description Nomuraea rileyi ARSEF 4094, an entomopathogenic fungus (EF) isolated from Spodoptera frugiperda, produces secondary metabolites (SM) that interfere with the development of the insects that attack and bacteria from the insect body. It is known that the addition of insects into the fungal growth medium stimulates the production of SM. SM are known to affect bacterial growth in their planktonic and biofilm (BF) states. Therefore, the aim of this study was to evaluate the inhibitory capacity of N. rileyi in the presence of S. frugiperda on Xanthomonas citri subsp. citri (Xcc) growth and BF formation. This bacterium causes citrus canker, the main crop in Tucumán.EF was cultured in SMY medium with S. frugiperda and incubated at 28 °C during 15 days, at 180 rpm. Fungal and insect controls with the same treatment were made. Ethyl acetate extracts from biomass and supernatant from each condition were obtained. Xcc growth inhibition was determined by the microdilution method at 48 h and the inhibition of BF formation was quantified by a colorimetric method.The extract from fungal-insect biomass was the most efficient in inhibiting BF formation (38%), affecting Xcc growth in only 15%. While the extract from fungal biomass inhibited the growth of the bacteria in 32% and only 9% the formation of BF. According to this, the presence of insects could stimulate the production of SM, which interfere with the synthesis of BF.The results showed the potential use of extracts from N. rileyi as a natural strategy and environmentally acceptable for crop protection against diseases caused by pathogenic microorganisms.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237048
Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri; XXXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafi del Valle; Argentina; 2016; 17-17
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237048
identifier_str_mv Nomuraea rileyi Inhibits growth and biofilm formation Xanthomonas citri subsp citri; XXXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tafi del Valle; Argentina; 2016; 17-17
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://asobioltuc.com/uploads/archivos/1503947552_QmlvY2VsbCBYWFhJSUkgSkMgMjAxNi5wZGY=.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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