The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation

Autores
Zimaro, Tamara; Thomas, Ludivine; Marondedze, Claudius; Sgro, Germán Gustavo; Garofalo, Cecilia Graciela; Ficarra, Florencia Andrea; Gehring, Chris; Ottado, Jorgelina; Gottig Schor, Natalia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein secretion system (T3SS). The role of T3SS in bacterial pathogenesis is well established but whether this system is involved in multicellular processes, such as bacterial biofilm formation has not been elucidated. Here, the phytopathogen Xanthomonas citri subsp. citri (X. citri) was used as a model to gain further insights about the role of the T3SS in biofilm formation. Results: The capacity of biofilm formation of different X. citri T3SS mutants was compared to the wild type strain and it was observed that this secretion system was necessary for this process. Moreover, the T3SS mutants adhered proficiently to leaf surfaces but were impaired in leaf-associated growth. A proteomic study of biofilm cells showed that the lack of the T3SS causes changes in the expression of proteins involved in metabolic processes, energy generation, exopolysaccharide (EPS) production and bacterial motility as well as outer membrane proteins. Furthermore, EPS production and bacterial motility were also altered in the T3SS mutants. Conclusions: Our results indicate a novel role for T3SS in X. citri in the modulation of biofilm formation. Since this process increases X. citri virulence, this study reveals new functions of T3SS in pathogenesis.
Fil: Zimaro, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Thomas, Ludivine. King Abdullah University Of Science And Technology;
Fil: Marondedze, Claudius. King Abdullah University Of Science And Technology;
Fil: Sgro, Germán Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Garofalo, Cecilia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ficarra, Florencia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gehring, Chris. King Abdullah University Of Science And Technology;
Fil: Ottado, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gottig Schor, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
XANTHOMONAS CITRI SUBSP CITRI
BIOFILM
T3SS
PROTEOMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8659
Acceder
id CONICETDig_55cc58de1132a1cdce7b1c741c281053
oai_identifier_str oai:ri.conicet.gov.ar:11336/8659
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formationZimaro, TamaraThomas, LudivineMarondedze, ClaudiusSgro, Germán GustavoGarofalo, Cecilia GracielaFicarra, Florencia AndreaGehring, ChrisOttado, JorgelinaGottig Schor, NataliaXANTHOMONAS CITRI SUBSP CITRIBIOFILMT3SSPROTEOMICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein secretion system (T3SS). The role of T3SS in bacterial pathogenesis is well established but whether this system is involved in multicellular processes, such as bacterial biofilm formation has not been elucidated. Here, the phytopathogen Xanthomonas citri subsp. citri (X. citri) was used as a model to gain further insights about the role of the T3SS in biofilm formation. Results: The capacity of biofilm formation of different X. citri T3SS mutants was compared to the wild type strain and it was observed that this secretion system was necessary for this process. Moreover, the T3SS mutants adhered proficiently to leaf surfaces but were impaired in leaf-associated growth. A proteomic study of biofilm cells showed that the lack of the T3SS causes changes in the expression of proteins involved in metabolic processes, energy generation, exopolysaccharide (EPS) production and bacterial motility as well as outer membrane proteins. Furthermore, EPS production and bacterial motility were also altered in the T3SS mutants. Conclusions: Our results indicate a novel role for T3SS in X. citri in the modulation of biofilm formation. Since this process increases X. citri virulence, this study reveals new functions of T3SS in pathogenesis.Fil: Zimaro, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Thomas, Ludivine. King Abdullah University Of Science And Technology;Fil: Marondedze, Claudius. King Abdullah University Of Science And Technology;Fil: Sgro, Germán Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Garofalo, Cecilia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Ficarra, Florencia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Gehring, Chris. King Abdullah University Of Science And Technology;Fil: Ottado, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Gottig Schor, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaBiomed Central2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8659Zimaro, Tamara; Thomas, Ludivine; Marondedze, Claudius; Sgro, Germán Gustavo; Garofalo, Cecilia Graciela; et al.; The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation; Biomed Central; Bmc Microbiology; 14; 96; 4-2014; 96-1111471-2180enginfo:eu-repo/semantics/altIdentifier/url/http://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-96info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-14-96info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24742141/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:37Zoai:ri.conicet.gov.ar:11336/8659instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:37.19CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation
title The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation
spellingShingle The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation
Zimaro, Tamara
XANTHOMONAS CITRI SUBSP CITRI
BIOFILM
T3SS
PROTEOMICS
title_short The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation
title_full The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation
title_fullStr The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation
title_full_unstemmed The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation
title_sort The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation
dc.creator.none.fl_str_mv Zimaro, Tamara
Thomas, Ludivine
Marondedze, Claudius
Sgro, Germán Gustavo
Garofalo, Cecilia Graciela
Ficarra, Florencia Andrea
Gehring, Chris
Ottado, Jorgelina
Gottig Schor, Natalia
author Zimaro, Tamara
author_facet Zimaro, Tamara
Thomas, Ludivine
Marondedze, Claudius
Sgro, Germán Gustavo
Garofalo, Cecilia Graciela
Ficarra, Florencia Andrea
Gehring, Chris
Ottado, Jorgelina
Gottig Schor, Natalia
author_role author
author2 Thomas, Ludivine
Marondedze, Claudius
Sgro, Germán Gustavo
Garofalo, Cecilia Graciela
Ficarra, Florencia Andrea
Gehring, Chris
Ottado, Jorgelina
Gottig Schor, Natalia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv XANTHOMONAS CITRI SUBSP CITRI
BIOFILM
T3SS
PROTEOMICS
topic XANTHOMONAS CITRI SUBSP CITRI
BIOFILM
T3SS
PROTEOMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein secretion system (T3SS). The role of T3SS in bacterial pathogenesis is well established but whether this system is involved in multicellular processes, such as bacterial biofilm formation has not been elucidated. Here, the phytopathogen Xanthomonas citri subsp. citri (X. citri) was used as a model to gain further insights about the role of the T3SS in biofilm formation. Results: The capacity of biofilm formation of different X. citri T3SS mutants was compared to the wild type strain and it was observed that this secretion system was necessary for this process. Moreover, the T3SS mutants adhered proficiently to leaf surfaces but were impaired in leaf-associated growth. A proteomic study of biofilm cells showed that the lack of the T3SS causes changes in the expression of proteins involved in metabolic processes, energy generation, exopolysaccharide (EPS) production and bacterial motility as well as outer membrane proteins. Furthermore, EPS production and bacterial motility were also altered in the T3SS mutants. Conclusions: Our results indicate a novel role for T3SS in X. citri in the modulation of biofilm formation. Since this process increases X. citri virulence, this study reveals new functions of T3SS in pathogenesis.
Fil: Zimaro, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Thomas, Ludivine. King Abdullah University Of Science And Technology;
Fil: Marondedze, Claudius. King Abdullah University Of Science And Technology;
Fil: Sgro, Germán Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Garofalo, Cecilia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ficarra, Florencia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gehring, Chris. King Abdullah University Of Science And Technology;
Fil: Ottado, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gottig Schor, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Background: Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein secretion system (T3SS). The role of T3SS in bacterial pathogenesis is well established but whether this system is involved in multicellular processes, such as bacterial biofilm formation has not been elucidated. Here, the phytopathogen Xanthomonas citri subsp. citri (X. citri) was used as a model to gain further insights about the role of the T3SS in biofilm formation. Results: The capacity of biofilm formation of different X. citri T3SS mutants was compared to the wild type strain and it was observed that this secretion system was necessary for this process. Moreover, the T3SS mutants adhered proficiently to leaf surfaces but were impaired in leaf-associated growth. A proteomic study of biofilm cells showed that the lack of the T3SS causes changes in the expression of proteins involved in metabolic processes, energy generation, exopolysaccharide (EPS) production and bacterial motility as well as outer membrane proteins. Furthermore, EPS production and bacterial motility were also altered in the T3SS mutants. Conclusions: Our results indicate a novel role for T3SS in X. citri in the modulation of biofilm formation. Since this process increases X. citri virulence, this study reveals new functions of T3SS in pathogenesis.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8659
Zimaro, Tamara; Thomas, Ludivine; Marondedze, Claudius; Sgro, Germán Gustavo; Garofalo, Cecilia Graciela; et al.; The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation; Biomed Central; Bmc Microbiology; 14; 96; 4-2014; 96-111
1471-2180
url http://hdl.handle.net/11336/8659
identifier_str_mv Zimaro, Tamara; Thomas, Ludivine; Marondedze, Claudius; Sgro, Germán Gustavo; Garofalo, Cecilia Graciela; et al.; The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation; Biomed Central; Bmc Microbiology; 14; 96; 4-2014; 96-111
1471-2180
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-96
info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-14-96
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24742141/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.004268

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