Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri

Autores
Luján, Enrique Ernesto; Torres Carro, Romina; Fogliata, Gabriela; Alberto, Maria Rosa; Arena, Mario Eduardo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
For the present work, Metarhizium rileyi, a common entomopathogenic fungus, was grown in batch conditions in the absence and presence of its host, Spodoptera frugiperda, to obtain secondary metabolites with potential antimicrobial effect. The extraction of secondary metabolites was carried out by using two solvent systems for the biomass (ethyl acetate and methanol), while secondary metabolites present in the supernatant were extracted by using ethyl acetate as extraction solvent. These extracts were evaluated for their inhibitory effect on the growth, biofilm formation, and motility of three Xanthomonas citri subsp. citri strains (Xcc20, Xcc29, and Xcc42). The in vivo effect of these extracts to prevent the development of cankers on grapefruit leaves was also evaluated.M. rileyi biomass ethyl acetate extracts in the presence and absence of its host had a strong inhibitory effect on Xcc strains growth. On the other hand, the presence of S. frugiperda as an elicitor significantly increased M. rileyi?s inhibitory capacity to form a biofilm. A different behavior was observed for the copper resistant strain, Xcc42, for its biofilm formation and swimming capacity since the most active samples were the supernatant extracts in the presence andabsence of S. frugiperda. Our results suggest that the most important factor for in vivo canker development is the inhibition of Xcc?s motility since all the extracts that inhibited canker development in vivo were also able to inhibit Xcc20?s motility in vitro. The fact that the extracts can prevent cankers development in vivo indicates they are promising sources of metabolites to prevent the development of this citrus disease.
Fil: Luján, Enrique Ernesto. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Torres Carro, Romina. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina
Fil: Fogliata, Gabriela. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Alberto, Maria Rosa. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Arena, Mario Eduardo. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
CITRUS CANKERS
METARHIZIUM RILEYI
SPODOPTERA FRUGIPERDA
XANTHOMONAS CITRI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121641
Acceder
id CONICETDig_58d7532dce7af9ee95834496ed3cf440
oai_identifier_str oai:ri.conicet.gov.ar:11336/121641
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citriLuján, Enrique ErnestoTorres Carro, RominaFogliata, GabrielaAlberto, Maria RosaArena, Mario EduardoCITRUS CANKERSMETARHIZIUM RILEYISPODOPTERA FRUGIPERDAXANTHOMONAS CITRIhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4For the present work, Metarhizium rileyi, a common entomopathogenic fungus, was grown in batch conditions in the absence and presence of its host, Spodoptera frugiperda, to obtain secondary metabolites with potential antimicrobial effect. The extraction of secondary metabolites was carried out by using two solvent systems for the biomass (ethyl acetate and methanol), while secondary metabolites present in the supernatant were extracted by using ethyl acetate as extraction solvent. These extracts were evaluated for their inhibitory effect on the growth, biofilm formation, and motility of three Xanthomonas citri subsp. citri strains (Xcc20, Xcc29, and Xcc42). The in vivo effect of these extracts to prevent the development of cankers on grapefruit leaves was also evaluated.M. rileyi biomass ethyl acetate extracts in the presence and absence of its host had a strong inhibitory effect on Xcc strains growth. On the other hand, the presence of S. frugiperda as an elicitor significantly increased M. rileyi?s inhibitory capacity to form a biofilm. A different behavior was observed for the copper resistant strain, Xcc42, for its biofilm formation and swimming capacity since the most active samples were the supernatant extracts in the presence andabsence of S. frugiperda. Our results suggest that the most important factor for in vivo canker development is the inhibition of Xcc?s motility since all the extracts that inhibited canker development in vivo were also able to inhibit Xcc20?s motility in vitro. The fact that the extracts can prevent cankers development in vivo indicates they are promising sources of metabolites to prevent the development of this citrus disease.Fil: Luján, Enrique Ernesto. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Torres Carro, Romina. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; ArgentinaFil: Fogliata, Gabriela. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; ArgentinaFil: Alberto, Maria Rosa. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Arena, Mario Eduardo. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaAvanti Publishers2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121641Luján, Enrique Ernesto; Torres Carro, Romina; Fogliata, Gabriela; Alberto, Maria Rosa; Arena, Mario Eduardo; Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri; Avanti Publishers; Global Journal of Agricultural Innovation, Research and Development; 6; 12-2019; 25-372409-98132409-9813CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.15377/2409-9813.2019.06.3info:eu-repo/semantics/altIdentifier/url/http://www.avantipublishers.com/downloads/gjairdv6a3/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:12:48Zoai:ri.conicet.gov.ar:11336/121641instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:12:49.02CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri
title Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri
spellingShingle Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri
Luján, Enrique Ernesto
CITRUS CANKERS
METARHIZIUM RILEYI
SPODOPTERA FRUGIPERDA
XANTHOMONAS CITRI
title_short Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri
title_full Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri
title_fullStr Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri
title_full_unstemmed Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri
title_sort Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri
dc.creator.none.fl_str_mv Luján, Enrique Ernesto
Torres Carro, Romina
Fogliata, Gabriela
Alberto, Maria Rosa
Arena, Mario Eduardo
author Luján, Enrique Ernesto
author_facet Luján, Enrique Ernesto
Torres Carro, Romina
Fogliata, Gabriela
Alberto, Maria Rosa
Arena, Mario Eduardo
author_role author
author2 Torres Carro, Romina
Fogliata, Gabriela
Alberto, Maria Rosa
Arena, Mario Eduardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv CITRUS CANKERS
METARHIZIUM RILEYI
SPODOPTERA FRUGIPERDA
XANTHOMONAS CITRI
topic CITRUS CANKERS
METARHIZIUM RILEYI
SPODOPTERA FRUGIPERDA
XANTHOMONAS CITRI
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv For the present work, Metarhizium rileyi, a common entomopathogenic fungus, was grown in batch conditions in the absence and presence of its host, Spodoptera frugiperda, to obtain secondary metabolites with potential antimicrobial effect. The extraction of secondary metabolites was carried out by using two solvent systems for the biomass (ethyl acetate and methanol), while secondary metabolites present in the supernatant were extracted by using ethyl acetate as extraction solvent. These extracts were evaluated for their inhibitory effect on the growth, biofilm formation, and motility of three Xanthomonas citri subsp. citri strains (Xcc20, Xcc29, and Xcc42). The in vivo effect of these extracts to prevent the development of cankers on grapefruit leaves was also evaluated.M. rileyi biomass ethyl acetate extracts in the presence and absence of its host had a strong inhibitory effect on Xcc strains growth. On the other hand, the presence of S. frugiperda as an elicitor significantly increased M. rileyi?s inhibitory capacity to form a biofilm. A different behavior was observed for the copper resistant strain, Xcc42, for its biofilm formation and swimming capacity since the most active samples were the supernatant extracts in the presence andabsence of S. frugiperda. Our results suggest that the most important factor for in vivo canker development is the inhibition of Xcc?s motility since all the extracts that inhibited canker development in vivo were also able to inhibit Xcc20?s motility in vitro. The fact that the extracts can prevent cankers development in vivo indicates they are promising sources of metabolites to prevent the development of this citrus disease.
Fil: Luján, Enrique Ernesto. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Torres Carro, Romina. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina
Fil: Fogliata, Gabriela. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Alberto, Maria Rosa. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Arena, Mario Eduardo. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description For the present work, Metarhizium rileyi, a common entomopathogenic fungus, was grown in batch conditions in the absence and presence of its host, Spodoptera frugiperda, to obtain secondary metabolites with potential antimicrobial effect. The extraction of secondary metabolites was carried out by using two solvent systems for the biomass (ethyl acetate and methanol), while secondary metabolites present in the supernatant were extracted by using ethyl acetate as extraction solvent. These extracts were evaluated for their inhibitory effect on the growth, biofilm formation, and motility of three Xanthomonas citri subsp. citri strains (Xcc20, Xcc29, and Xcc42). The in vivo effect of these extracts to prevent the development of cankers on grapefruit leaves was also evaluated.M. rileyi biomass ethyl acetate extracts in the presence and absence of its host had a strong inhibitory effect on Xcc strains growth. On the other hand, the presence of S. frugiperda as an elicitor significantly increased M. rileyi?s inhibitory capacity to form a biofilm. A different behavior was observed for the copper resistant strain, Xcc42, for its biofilm formation and swimming capacity since the most active samples were the supernatant extracts in the presence andabsence of S. frugiperda. Our results suggest that the most important factor for in vivo canker development is the inhibition of Xcc?s motility since all the extracts that inhibited canker development in vivo were also able to inhibit Xcc20?s motility in vitro. The fact that the extracts can prevent cankers development in vivo indicates they are promising sources of metabolites to prevent the development of this citrus disease.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121641
Luján, Enrique Ernesto; Torres Carro, Romina; Fogliata, Gabriela; Alberto, Maria Rosa; Arena, Mario Eduardo; Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri; Avanti Publishers; Global Journal of Agricultural Innovation, Research and Development; 6; 12-2019; 25-37
2409-9813
2409-9813
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121641
identifier_str_mv Luján, Enrique Ernesto; Torres Carro, Romina; Fogliata, Gabriela; Alberto, Maria Rosa; Arena, Mario Eduardo; Fungal extracts as biocontrol of growth, biofilm formation, and motility of xanthomonas citri subsp. citri; Avanti Publishers; Global Journal of Agricultural Innovation, Research and Development; 6; 12-2019; 25-37
2409-9813
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.15377/2409-9813.2019.06.3
info:eu-repo/semantics/altIdentifier/url/http://www.avantipublishers.com/downloads/gjairdv6a3/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Avanti Publishers
publisher.none.fl_str_mv Avanti Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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