AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain
- Autores
- Seguin, Leonardo Roque; Villarreal, Rodrigo Sebastian; Ciuffo, Gladys Maria
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The functional role of AT 2 receptors is unclear and it activates unconventional signaling pathways, which in general do not involve a classical activation of a G-protein. In the present study, we aimed to investigate the transduction mechanism of AT 2 Ang II receptors in PND15 rat hindbrain membrane preparations, which represents a physiological developmental condition. To determine whether Ang II AT 2 receptors induced association to SHP-1 in rat hindbrain, co-immunoprecipitation assays were performed. Stimulation of Ang II AT 2 receptors induced both a transient tyr-phosphorylation and activation of SHP-1. The possible participation of c-Src in Ang II-mediated SHP-1 activation, we demonstrated by recruitment of c-Src in immunocomplexes obtained with anti AT 2 or anti-SHP-1 antibodies. The association of SHP-1 to c-Src was inhibited by PD123319 and the c-Src inhibitor PP2. Similarly, SHP-1 activity determined in AT 2-immunocomplexes was inhibited by PD123319 and the c-Src inhibitor PP2. Following stimulation with Ang II, AT 2 receptors recruit c-Src, which was responsible for SHP-1 tyr-phosphorylation and activation. Since AT 2 receptors are involved in neuron migration, we tested the presence of FAK in immunocomplexes. Surprisingly, AT 2-immunocomplexes contained mainly the 85 kDa fragment of FAK. Besides, p125FAK associated to SHP-1. In summary, we demonstrated the presence of an active signal transduction mechanism in PND15 rat hindbrain, a developmental stage critical for cerebellar development. In this model, we showed a complex containing AT 2/SHP-1/c-Src/p85FAK, suggesting a potential role of Ang II AT 2 receptors in cerebellar development and neuronal differentiation.
Fil: Seguin, Leonardo Roque. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Villarreal, Rodrigo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Ciuffo, Gladys Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina - Materia
-
AT 2 RECEPTORS
FOCAL ADHESION KINASE
HINDBRAIN DEVELOPMENT
SHP-1/C-SRC INTERACTION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/125889
Ver los metadatos del registro completo
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AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrainSeguin, Leonardo RoqueVillarreal, Rodrigo SebastianCiuffo, Gladys MariaAT 2 RECEPTORSFOCAL ADHESION KINASEHINDBRAIN DEVELOPMENTSHP-1/C-SRC INTERACTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The functional role of AT 2 receptors is unclear and it activates unconventional signaling pathways, which in general do not involve a classical activation of a G-protein. In the present study, we aimed to investigate the transduction mechanism of AT 2 Ang II receptors in PND15 rat hindbrain membrane preparations, which represents a physiological developmental condition. To determine whether Ang II AT 2 receptors induced association to SHP-1 in rat hindbrain, co-immunoprecipitation assays were performed. Stimulation of Ang II AT 2 receptors induced both a transient tyr-phosphorylation and activation of SHP-1. The possible participation of c-Src in Ang II-mediated SHP-1 activation, we demonstrated by recruitment of c-Src in immunocomplexes obtained with anti AT 2 or anti-SHP-1 antibodies. The association of SHP-1 to c-Src was inhibited by PD123319 and the c-Src inhibitor PP2. Similarly, SHP-1 activity determined in AT 2-immunocomplexes was inhibited by PD123319 and the c-Src inhibitor PP2. Following stimulation with Ang II, AT 2 receptors recruit c-Src, which was responsible for SHP-1 tyr-phosphorylation and activation. Since AT 2 receptors are involved in neuron migration, we tested the presence of FAK in immunocomplexes. Surprisingly, AT 2-immunocomplexes contained mainly the 85 kDa fragment of FAK. Besides, p125FAK associated to SHP-1. In summary, we demonstrated the presence of an active signal transduction mechanism in PND15 rat hindbrain, a developmental stage critical for cerebellar development. In this model, we showed a complex containing AT 2/SHP-1/c-Src/p85FAK, suggesting a potential role of Ang II AT 2 receptors in cerebellar development and neuronal differentiation.Fil: Seguin, Leonardo Roque. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Villarreal, Rodrigo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Ciuffo, Gladys Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaPergamon-Elsevier Science Ltd2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125889Seguin, Leonardo Roque; Villarreal, Rodrigo Sebastian; Ciuffo, Gladys Maria; AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain; Pergamon-Elsevier Science Ltd; Neurochemistry International; 60; 2; 1-2012; 199-2070197-0186CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0197018611003731info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2011.11.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:03:24Zoai:ri.conicet.gov.ar:11336/125889instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:03:24.583CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain |
| title |
AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain |
| spellingShingle |
AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain Seguin, Leonardo Roque AT 2 RECEPTORS FOCAL ADHESION KINASE HINDBRAIN DEVELOPMENT SHP-1/C-SRC INTERACTION |
| title_short |
AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain |
| title_full |
AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain |
| title_fullStr |
AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain |
| title_full_unstemmed |
AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain |
| title_sort |
AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain |
| dc.creator.none.fl_str_mv |
Seguin, Leonardo Roque Villarreal, Rodrigo Sebastian Ciuffo, Gladys Maria |
| author |
Seguin, Leonardo Roque |
| author_facet |
Seguin, Leonardo Roque Villarreal, Rodrigo Sebastian Ciuffo, Gladys Maria |
| author_role |
author |
| author2 |
Villarreal, Rodrigo Sebastian Ciuffo, Gladys Maria |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
AT 2 RECEPTORS FOCAL ADHESION KINASE HINDBRAIN DEVELOPMENT SHP-1/C-SRC INTERACTION |
| topic |
AT 2 RECEPTORS FOCAL ADHESION KINASE HINDBRAIN DEVELOPMENT SHP-1/C-SRC INTERACTION |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
The functional role of AT 2 receptors is unclear and it activates unconventional signaling pathways, which in general do not involve a classical activation of a G-protein. In the present study, we aimed to investigate the transduction mechanism of AT 2 Ang II receptors in PND15 rat hindbrain membrane preparations, which represents a physiological developmental condition. To determine whether Ang II AT 2 receptors induced association to SHP-1 in rat hindbrain, co-immunoprecipitation assays were performed. Stimulation of Ang II AT 2 receptors induced both a transient tyr-phosphorylation and activation of SHP-1. The possible participation of c-Src in Ang II-mediated SHP-1 activation, we demonstrated by recruitment of c-Src in immunocomplexes obtained with anti AT 2 or anti-SHP-1 antibodies. The association of SHP-1 to c-Src was inhibited by PD123319 and the c-Src inhibitor PP2. Similarly, SHP-1 activity determined in AT 2-immunocomplexes was inhibited by PD123319 and the c-Src inhibitor PP2. Following stimulation with Ang II, AT 2 receptors recruit c-Src, which was responsible for SHP-1 tyr-phosphorylation and activation. Since AT 2 receptors are involved in neuron migration, we tested the presence of FAK in immunocomplexes. Surprisingly, AT 2-immunocomplexes contained mainly the 85 kDa fragment of FAK. Besides, p125FAK associated to SHP-1. In summary, we demonstrated the presence of an active signal transduction mechanism in PND15 rat hindbrain, a developmental stage critical for cerebellar development. In this model, we showed a complex containing AT 2/SHP-1/c-Src/p85FAK, suggesting a potential role of Ang II AT 2 receptors in cerebellar development and neuronal differentiation. Fil: Seguin, Leonardo Roque. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina Fil: Villarreal, Rodrigo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina Fil: Ciuffo, Gladys Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina |
| description |
The functional role of AT 2 receptors is unclear and it activates unconventional signaling pathways, which in general do not involve a classical activation of a G-protein. In the present study, we aimed to investigate the transduction mechanism of AT 2 Ang II receptors in PND15 rat hindbrain membrane preparations, which represents a physiological developmental condition. To determine whether Ang II AT 2 receptors induced association to SHP-1 in rat hindbrain, co-immunoprecipitation assays were performed. Stimulation of Ang II AT 2 receptors induced both a transient tyr-phosphorylation and activation of SHP-1. The possible participation of c-Src in Ang II-mediated SHP-1 activation, we demonstrated by recruitment of c-Src in immunocomplexes obtained with anti AT 2 or anti-SHP-1 antibodies. The association of SHP-1 to c-Src was inhibited by PD123319 and the c-Src inhibitor PP2. Similarly, SHP-1 activity determined in AT 2-immunocomplexes was inhibited by PD123319 and the c-Src inhibitor PP2. Following stimulation with Ang II, AT 2 receptors recruit c-Src, which was responsible for SHP-1 tyr-phosphorylation and activation. Since AT 2 receptors are involved in neuron migration, we tested the presence of FAK in immunocomplexes. Surprisingly, AT 2-immunocomplexes contained mainly the 85 kDa fragment of FAK. Besides, p125FAK associated to SHP-1. In summary, we demonstrated the presence of an active signal transduction mechanism in PND15 rat hindbrain, a developmental stage critical for cerebellar development. In this model, we showed a complex containing AT 2/SHP-1/c-Src/p85FAK, suggesting a potential role of Ang II AT 2 receptors in cerebellar development and neuronal differentiation. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-01 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/125889 Seguin, Leonardo Roque; Villarreal, Rodrigo Sebastian; Ciuffo, Gladys Maria; AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain; Pergamon-Elsevier Science Ltd; Neurochemistry International; 60; 2; 1-2012; 199-207 0197-0186 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/125889 |
| identifier_str_mv |
Seguin, Leonardo Roque; Villarreal, Rodrigo Sebastian; Ciuffo, Gladys Maria; AT 2 receptors recruit c-Src, SHP-1 and FAK upon activation by Ang II in PND15 rat hindbrain; Pergamon-Elsevier Science Ltd; Neurochemistry International; 60; 2; 1-2012; 199-207 0197-0186 CONICET Digital CONICET |
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eng |
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Pergamon-Elsevier Science Ltd |
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Pergamon-Elsevier Science Ltd |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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