Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase

Autores
Flamini, Marina Ines; Sanchez, Angel Matias; Genazzani, Andrea Riccardo; Simoncini, Tommaso
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To explore the effects of 17β-estradiol (E2) on cytoskeletal remodeling and motility of endometrial stromal cells (ESC) and Ishikawa cells and to characterize the role of focal adhesion kinase (FAK) in these processes. Design: In vitro study of cytoskeletal remodeling and cellular morphology and motility in ESC or Ishikawa cells. Setting: University research center. Patient(s): Endometrial samples obtained from women requiring endometrial biopsies. Intervention(s): Treatments with E2 and multiple inhibitors of signaling pathways. Main Outcome Measure(s): Activation of FAK, actin remodeling, membrane morphology, cell motility, and invasion. Result(s): Estrogen induces a rapid and concentration-related FAK phosphorylation in ESC and Ishikawa cells. In this time frame, FAK localizes to the plasma membrane at sites of focal adhesion complexes formation, as shown by immunofluorescence. Phosphorylation of FAK in the presence of estrogen depends on the recruitment of both estrogen receptor α and estrogen receptor β and of a rapid G protein-dependent signaling to c-Src and phosphatidylinositol 3-OH kinase. Activation of FAK in ESC and Ishikawa cells is required for estrogen-induced horizontal migration and invasion of three-dimensional matrices of endometrial cells. Conclusion(s): Estrogen enhances cytoskeletal and membrane remodeling in ESC and Ishikawa cells by controlling FAK, thus resulting in enhanced cell motility and invasion. These findings may have clinical relevance for the development of new therapeutic strategies for the prevention or control of endometrial diseases.
Fil: Flamini, Marina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Università degli Studi di Pisa; Italia
Fil: Sanchez, Angel Matias. Università degli Studi di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Genazzani, Andrea Riccardo. Università degli Studi di Pisa; Italia
Fil: Simoncini, Tommaso. Università degli Studi di Pisa; Italia
Materia
Actin Cytoskeleton
Endometrial Cells
Estrogen
Focal Adhesion Complexes
Focal Adhesion Kinase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/80371
Acceder
id CONICETDig_4171317c2ca9453b413a1169daf36aab
oai_identifier_str oai:ri.conicet.gov.ar:11336/80371
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinaseFlamini, Marina InesSanchez, Angel MatiasGenazzani, Andrea RiccardoSimoncini, TommasoActin CytoskeletonEndometrial CellsEstrogenFocal Adhesion ComplexesFocal Adhesion Kinasehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Objective: To explore the effects of 17β-estradiol (E2) on cytoskeletal remodeling and motility of endometrial stromal cells (ESC) and Ishikawa cells and to characterize the role of focal adhesion kinase (FAK) in these processes. Design: In vitro study of cytoskeletal remodeling and cellular morphology and motility in ESC or Ishikawa cells. Setting: University research center. Patient(s): Endometrial samples obtained from women requiring endometrial biopsies. Intervention(s): Treatments with E2 and multiple inhibitors of signaling pathways. Main Outcome Measure(s): Activation of FAK, actin remodeling, membrane morphology, cell motility, and invasion. Result(s): Estrogen induces a rapid and concentration-related FAK phosphorylation in ESC and Ishikawa cells. In this time frame, FAK localizes to the plasma membrane at sites of focal adhesion complexes formation, as shown by immunofluorescence. Phosphorylation of FAK in the presence of estrogen depends on the recruitment of both estrogen receptor α and estrogen receptor β and of a rapid G protein-dependent signaling to c-Src and phosphatidylinositol 3-OH kinase. Activation of FAK in ESC and Ishikawa cells is required for estrogen-induced horizontal migration and invasion of three-dimensional matrices of endometrial cells. Conclusion(s): Estrogen enhances cytoskeletal and membrane remodeling in ESC and Ishikawa cells by controlling FAK, thus resulting in enhanced cell motility and invasion. These findings may have clinical relevance for the development of new therapeutic strategies for the prevention or control of endometrial diseases.Fil: Flamini, Marina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Università degli Studi di Pisa; ItaliaFil: Sanchez, Angel Matias. Università degli Studi di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Genazzani, Andrea Riccardo. Università degli Studi di Pisa; ItaliaFil: Simoncini, Tommaso. Università degli Studi di Pisa; ItaliaElsevier Science Inc2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80371Flamini, Marina Ines; Sanchez, Angel Matias; Genazzani, Andrea Riccardo; Simoncini, Tommaso; Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase; Elsevier Science Inc; Fertility and Sterility; 95; 2; 2-2011; 722-7260015-0282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2010.08.039info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0015028210024532info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:04Zoai:ri.conicet.gov.ar:11336/80371instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:04.535CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase
title Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase
spellingShingle Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase
Flamini, Marina Ines
Actin Cytoskeleton
Endometrial Cells
Estrogen
Focal Adhesion Complexes
Focal Adhesion Kinase
title_short Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase
title_full Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase
title_fullStr Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase
title_full_unstemmed Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase
title_sort Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase
dc.creator.none.fl_str_mv Flamini, Marina Ines
Sanchez, Angel Matias
Genazzani, Andrea Riccardo
Simoncini, Tommaso
author Flamini, Marina Ines
author_facet Flamini, Marina Ines
Sanchez, Angel Matias
Genazzani, Andrea Riccardo
Simoncini, Tommaso
author_role author
author2 Sanchez, Angel Matias
Genazzani, Andrea Riccardo
Simoncini, Tommaso
author2_role author
author
author
dc.subject.none.fl_str_mv Actin Cytoskeleton
Endometrial Cells
Estrogen
Focal Adhesion Complexes
Focal Adhesion Kinase
topic Actin Cytoskeleton
Endometrial Cells
Estrogen
Focal Adhesion Complexes
Focal Adhesion Kinase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: To explore the effects of 17β-estradiol (E2) on cytoskeletal remodeling and motility of endometrial stromal cells (ESC) and Ishikawa cells and to characterize the role of focal adhesion kinase (FAK) in these processes. Design: In vitro study of cytoskeletal remodeling and cellular morphology and motility in ESC or Ishikawa cells. Setting: University research center. Patient(s): Endometrial samples obtained from women requiring endometrial biopsies. Intervention(s): Treatments with E2 and multiple inhibitors of signaling pathways. Main Outcome Measure(s): Activation of FAK, actin remodeling, membrane morphology, cell motility, and invasion. Result(s): Estrogen induces a rapid and concentration-related FAK phosphorylation in ESC and Ishikawa cells. In this time frame, FAK localizes to the plasma membrane at sites of focal adhesion complexes formation, as shown by immunofluorescence. Phosphorylation of FAK in the presence of estrogen depends on the recruitment of both estrogen receptor α and estrogen receptor β and of a rapid G protein-dependent signaling to c-Src and phosphatidylinositol 3-OH kinase. Activation of FAK in ESC and Ishikawa cells is required for estrogen-induced horizontal migration and invasion of three-dimensional matrices of endometrial cells. Conclusion(s): Estrogen enhances cytoskeletal and membrane remodeling in ESC and Ishikawa cells by controlling FAK, thus resulting in enhanced cell motility and invasion. These findings may have clinical relevance for the development of new therapeutic strategies for the prevention or control of endometrial diseases.
Fil: Flamini, Marina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Università degli Studi di Pisa; Italia
Fil: Sanchez, Angel Matias. Università degli Studi di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Genazzani, Andrea Riccardo. Università degli Studi di Pisa; Italia
Fil: Simoncini, Tommaso. Università degli Studi di Pisa; Italia
description Objective: To explore the effects of 17β-estradiol (E2) on cytoskeletal remodeling and motility of endometrial stromal cells (ESC) and Ishikawa cells and to characterize the role of focal adhesion kinase (FAK) in these processes. Design: In vitro study of cytoskeletal remodeling and cellular morphology and motility in ESC or Ishikawa cells. Setting: University research center. Patient(s): Endometrial samples obtained from women requiring endometrial biopsies. Intervention(s): Treatments with E2 and multiple inhibitors of signaling pathways. Main Outcome Measure(s): Activation of FAK, actin remodeling, membrane morphology, cell motility, and invasion. Result(s): Estrogen induces a rapid and concentration-related FAK phosphorylation in ESC and Ishikawa cells. In this time frame, FAK localizes to the plasma membrane at sites of focal adhesion complexes formation, as shown by immunofluorescence. Phosphorylation of FAK in the presence of estrogen depends on the recruitment of both estrogen receptor α and estrogen receptor β and of a rapid G protein-dependent signaling to c-Src and phosphatidylinositol 3-OH kinase. Activation of FAK in ESC and Ishikawa cells is required for estrogen-induced horizontal migration and invasion of three-dimensional matrices of endometrial cells. Conclusion(s): Estrogen enhances cytoskeletal and membrane remodeling in ESC and Ishikawa cells by controlling FAK, thus resulting in enhanced cell motility and invasion. These findings may have clinical relevance for the development of new therapeutic strategies for the prevention or control of endometrial diseases.
publishDate 2011
dc.date.none.fl_str_mv 2011-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/80371
Flamini, Marina Ines; Sanchez, Angel Matias; Genazzani, Andrea Riccardo; Simoncini, Tommaso; Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase; Elsevier Science Inc; Fertility and Sterility; 95; 2; 2-2011; 722-726
0015-0282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/80371
identifier_str_mv Flamini, Marina Ines; Sanchez, Angel Matias; Genazzani, Andrea Riccardo; Simoncini, Tommaso; Estrogen regulates endometrial cell cytoskeletal remodeling and motility via focal adhesion kinase; Elsevier Science Inc; Fertility and Sterility; 95; 2; 2-2011; 722-726
0015-0282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2010.08.039
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0015028210024532
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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