cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit

Autores
Krapf, Dario; Chun Ruan, Ye; Wertheimer Hermitte, Eva Victoria; Battistone, Maria Agustina; Pawlak, John B.; Sanjay, Archana; Pilder, Stephen H.; Cuasnicu, Patricia Sara; Breton, Sylvie; Visconti, Pablo E.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Changes that occur to mammalian sperm upon epididymal transit and maturation render these cells capable of moving progressively and capacitating. Signaling events leading to mammalian sperm capacitation depend on the modulation of proteins by phosphorylation and dephosphorylation cascades. Recent experiments have demonstrated that the Src family of kinases plays an important role in the regulation of these events. However, sperm from cSrc null mice display normal tyrosine phosphorylation associated with capacitation. We report here that, despite normal phosphorylation, sperm from cSrc null mice display a severe reduction in forward motility, and are unable to fertilize in vitro. Histological analysis of seminiferous tubules in the testes, caput and corpus epididymis do not reveal obvious defects. However, the cauda epididymis is significantly smaller, and expression of key transport proteins in the epithelial cells lining this region is reduced in cSrc null mice compared to wild type littermates. Although previously, we and others have shown the presence of cSrc in mature sperm from cauda epididymis, a closer evaluation indicates that this tyrosine kinase is not present in sperm from the caput epididymis, suggesting that this protein is acquired by sperm later during epididymal maturation. Consistent with this observation, cSrc is enriched in vesicles released by the epididymal epithelium known as epididymosomes. Altogether, these observations indicate that cSrc is essential for cauda epididymal development and suggest an essential role of this kinase in epididymal sperm maturation involving cSrc extracellular trafficking.
Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. University of Massachussets; Estados Unidos
Fil: Chun Ruan, Ye. Harvard Medical School; Estados Unidos
Fil: Wertheimer Hermitte, Eva Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. University of Massachussets; Estados Unidos
Fil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pawlak, John B.. University of Massachussets; Estados Unidos
Fil: Sanjay, Archana. University of Connecticut; Estados Unidos
Fil: Pilder, Stephen H.. Temple University; Estados Unidos
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Breton, Sylvie. Harvard Medical School; Estados Unidos
Fil: Visconti, Pablo E.. University of Massachussets; Estados Unidos
Materia
SRC KINASE
EPIDYDIMAL MATURATION
SPERM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271628
Acceder
id CONICETDig_13ede14e31b753eeb206d58eda7ea3fe
oai_identifier_str oai:ri.conicet.gov.ar:11336/271628
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transitKrapf, DarioChun Ruan, YeWertheimer Hermitte, Eva VictoriaBattistone, Maria AgustinaPawlak, John B.Sanjay, ArchanaPilder, Stephen H.Cuasnicu, Patricia SaraBreton, SylvieVisconti, Pablo E.SRC KINASEEPIDYDIMAL MATURATIONSPERMhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Changes that occur to mammalian sperm upon epididymal transit and maturation render these cells capable of moving progressively and capacitating. Signaling events leading to mammalian sperm capacitation depend on the modulation of proteins by phosphorylation and dephosphorylation cascades. Recent experiments have demonstrated that the Src family of kinases plays an important role in the regulation of these events. However, sperm from cSrc null mice display normal tyrosine phosphorylation associated with capacitation. We report here that, despite normal phosphorylation, sperm from cSrc null mice display a severe reduction in forward motility, and are unable to fertilize in vitro. Histological analysis of seminiferous tubules in the testes, caput and corpus epididymis do not reveal obvious defects. However, the cauda epididymis is significantly smaller, and expression of key transport proteins in the epithelial cells lining this region is reduced in cSrc null mice compared to wild type littermates. Although previously, we and others have shown the presence of cSrc in mature sperm from cauda epididymis, a closer evaluation indicates that this tyrosine kinase is not present in sperm from the caput epididymis, suggesting that this protein is acquired by sperm later during epididymal maturation. Consistent with this observation, cSrc is enriched in vesicles released by the epididymal epithelium known as epididymosomes. Altogether, these observations indicate that cSrc is essential for cauda epididymal development and suggest an essential role of this kinase in epididymal sperm maturation involving cSrc extracellular trafficking.Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. University of Massachussets; Estados UnidosFil: Chun Ruan, Ye. Harvard Medical School; Estados UnidosFil: Wertheimer Hermitte, Eva Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. University of Massachussets; Estados UnidosFil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pawlak, John B.. University of Massachussets; Estados UnidosFil: Sanjay, Archana. University of Connecticut; Estados UnidosFil: Pilder, Stephen H.. Temple University; Estados UnidosFil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Breton, Sylvie. Harvard Medical School; Estados UnidosFil: Visconti, Pablo E.. University of Massachussets; Estados UnidosAcademic Press Inc Elsevier Science2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271628Krapf, Dario; Chun Ruan, Ye; Wertheimer Hermitte, Eva Victoria; Battistone, Maria Agustina; Pawlak, John B.; et al.; cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit; Academic Press Inc Elsevier Science; Developmental Biology; 369; 1; 9-2012; 43-530012-1606CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0012160612003466info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2012.06.017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:53Zoai:ri.conicet.gov.ar:11336/271628instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:54.064CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit
title cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit
spellingShingle cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit
Krapf, Dario
SRC KINASE
EPIDYDIMAL MATURATION
SPERM
title_short cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit
title_full cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit
title_fullStr cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit
title_full_unstemmed cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit
title_sort cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit
dc.creator.none.fl_str_mv Krapf, Dario
Chun Ruan, Ye
Wertheimer Hermitte, Eva Victoria
Battistone, Maria Agustina
Pawlak, John B.
Sanjay, Archana
Pilder, Stephen H.
Cuasnicu, Patricia Sara
Breton, Sylvie
Visconti, Pablo E.
author Krapf, Dario
author_facet Krapf, Dario
Chun Ruan, Ye
Wertheimer Hermitte, Eva Victoria
Battistone, Maria Agustina
Pawlak, John B.
Sanjay, Archana
Pilder, Stephen H.
Cuasnicu, Patricia Sara
Breton, Sylvie
Visconti, Pablo E.
author_role author
author2 Chun Ruan, Ye
Wertheimer Hermitte, Eva Victoria
Battistone, Maria Agustina
Pawlak, John B.
Sanjay, Archana
Pilder, Stephen H.
Cuasnicu, Patricia Sara
Breton, Sylvie
Visconti, Pablo E.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SRC KINASE
EPIDYDIMAL MATURATION
SPERM
topic SRC KINASE
EPIDYDIMAL MATURATION
SPERM
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Changes that occur to mammalian sperm upon epididymal transit and maturation render these cells capable of moving progressively and capacitating. Signaling events leading to mammalian sperm capacitation depend on the modulation of proteins by phosphorylation and dephosphorylation cascades. Recent experiments have demonstrated that the Src family of kinases plays an important role in the regulation of these events. However, sperm from cSrc null mice display normal tyrosine phosphorylation associated with capacitation. We report here that, despite normal phosphorylation, sperm from cSrc null mice display a severe reduction in forward motility, and are unable to fertilize in vitro. Histological analysis of seminiferous tubules in the testes, caput and corpus epididymis do not reveal obvious defects. However, the cauda epididymis is significantly smaller, and expression of key transport proteins in the epithelial cells lining this region is reduced in cSrc null mice compared to wild type littermates. Although previously, we and others have shown the presence of cSrc in mature sperm from cauda epididymis, a closer evaluation indicates that this tyrosine kinase is not present in sperm from the caput epididymis, suggesting that this protein is acquired by sperm later during epididymal maturation. Consistent with this observation, cSrc is enriched in vesicles released by the epididymal epithelium known as epididymosomes. Altogether, these observations indicate that cSrc is essential for cauda epididymal development and suggest an essential role of this kinase in epididymal sperm maturation involving cSrc extracellular trafficking.
Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. University of Massachussets; Estados Unidos
Fil: Chun Ruan, Ye. Harvard Medical School; Estados Unidos
Fil: Wertheimer Hermitte, Eva Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. University of Massachussets; Estados Unidos
Fil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pawlak, John B.. University of Massachussets; Estados Unidos
Fil: Sanjay, Archana. University of Connecticut; Estados Unidos
Fil: Pilder, Stephen H.. Temple University; Estados Unidos
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Breton, Sylvie. Harvard Medical School; Estados Unidos
Fil: Visconti, Pablo E.. University of Massachussets; Estados Unidos
description Changes that occur to mammalian sperm upon epididymal transit and maturation render these cells capable of moving progressively and capacitating. Signaling events leading to mammalian sperm capacitation depend on the modulation of proteins by phosphorylation and dephosphorylation cascades. Recent experiments have demonstrated that the Src family of kinases plays an important role in the regulation of these events. However, sperm from cSrc null mice display normal tyrosine phosphorylation associated with capacitation. We report here that, despite normal phosphorylation, sperm from cSrc null mice display a severe reduction in forward motility, and are unable to fertilize in vitro. Histological analysis of seminiferous tubules in the testes, caput and corpus epididymis do not reveal obvious defects. However, the cauda epididymis is significantly smaller, and expression of key transport proteins in the epithelial cells lining this region is reduced in cSrc null mice compared to wild type littermates. Although previously, we and others have shown the presence of cSrc in mature sperm from cauda epididymis, a closer evaluation indicates that this tyrosine kinase is not present in sperm from the caput epididymis, suggesting that this protein is acquired by sperm later during epididymal maturation. Consistent with this observation, cSrc is enriched in vesicles released by the epididymal epithelium known as epididymosomes. Altogether, these observations indicate that cSrc is essential for cauda epididymal development and suggest an essential role of this kinase in epididymal sperm maturation involving cSrc extracellular trafficking.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271628
Krapf, Dario; Chun Ruan, Ye; Wertheimer Hermitte, Eva Victoria; Battistone, Maria Agustina; Pawlak, John B.; et al.; cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit; Academic Press Inc Elsevier Science; Developmental Biology; 369; 1; 9-2012; 43-53
0012-1606
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271628
identifier_str_mv Krapf, Dario; Chun Ruan, Ye; Wertheimer Hermitte, Eva Victoria; Battistone, Maria Agustina; Pawlak, John B.; et al.; cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit; Academic Press Inc Elsevier Science; Developmental Biology; 369; 1; 9-2012; 43-53
0012-1606
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0012160612003466
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2012.06.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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