Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae

Autores
Vargas, Juan Martin; Moreno Mochi, María Paula; Nuñez, J. M.; Cáceres, M.; Mochi, S.; del Campo Moreno, R.; Jure, Maria Angela
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Carbapenemase-producing Klebsiella pneumoniae (CRKP) are increasingly reported worldwide being necessary the local epidemiological monitoring. Our aim was to characterize the hypermucoviscous CRKP isolates collected in our hospital during a 6 months period. Carriage of the carbapenemase genes (blaKPC, blaNDM, blaVIM and blaOXA-48), extended spectrum β-lactamases (blaSHV-2, blaCTX-M) and the virulence genes (magA, k2A, rmpA, wabG, uge, allS, entB, ycfM, kpn, wcaG, fimH, mrkD, iutA, iroN, hly and cnf-1) were determined by multiplex-PCR. Genetic relationship among the isolates was performed by PFGE and MLST. A total of 35 isolates were recovered, being the urinary and respiratory tract the most common infection sites (34.2%). The blaKPC-2 gene was present in all the isolates, coexisting with blaCTX-M-2 (45.7%), blaSHV-2 (28.6%), and blaCTX-M-2/blaSHV-2 (14.3%). The capsular serotype K2 corresponded with 68.6% of the isolates. Virulence factors frequency were variable [adhesins (97.1%), siderophores (94.3%) and phagocytosis resistance (wabG 48.5%, uge 80% and ycfM 57.1%)]. A total of 10 STs were identified although 40% of them clustered on ST25-CC65, and 17% to ST17. The incidence of KPC-2-producing K. pneumoniae reported by the hospital was 0.290 per 1000 admissions. In summary we described an epidemic scenario of multidrug resistant hypermucoviscous KPC-2 producing ST25 K. pneumoniae in our institution.
Fil: Vargas, Juan Martin. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Moreno Mochi, María Paula. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Nuñez, J. M.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; Argentina
Fil: Cáceres, M.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; Argentina
Fil: Mochi, S.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; Argentina
Fil: del Campo Moreno, R.. Instituto Ramón y Cajal de Investigación Sanitaria; España
Fil: Jure, Maria Angela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; Argentina
Materia
CARBAPENEMASE
EPIDEMIOLOGY
INFECTIOUS DISEASE
KLEBSIELLA PNEUMONIAE
KPC
MICROBIOLOGY
MULTI-CLONAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/219138
Acceder
id CONICETDig_a173b7c0b4131e41845d61c716214274
oai_identifier_str oai:ri.conicet.gov.ar:11336/219138
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniaeVargas, Juan MartinMoreno Mochi, María PaulaNuñez, J. M.Cáceres, M.Mochi, S.del Campo Moreno, R.Jure, Maria AngelaCARBAPENEMASEEPIDEMIOLOGYINFECTIOUS DISEASEKLEBSIELLA PNEUMONIAEKPCMICROBIOLOGYMULTI-CLONALhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Carbapenemase-producing Klebsiella pneumoniae (CRKP) are increasingly reported worldwide being necessary the local epidemiological monitoring. Our aim was to characterize the hypermucoviscous CRKP isolates collected in our hospital during a 6 months period. Carriage of the carbapenemase genes (blaKPC, blaNDM, blaVIM and blaOXA-48), extended spectrum β-lactamases (blaSHV-2, blaCTX-M) and the virulence genes (magA, k2A, rmpA, wabG, uge, allS, entB, ycfM, kpn, wcaG, fimH, mrkD, iutA, iroN, hly and cnf-1) were determined by multiplex-PCR. Genetic relationship among the isolates was performed by PFGE and MLST. A total of 35 isolates were recovered, being the urinary and respiratory tract the most common infection sites (34.2%). The blaKPC-2 gene was present in all the isolates, coexisting with blaCTX-M-2 (45.7%), blaSHV-2 (28.6%), and blaCTX-M-2/blaSHV-2 (14.3%). The capsular serotype K2 corresponded with 68.6% of the isolates. Virulence factors frequency were variable [adhesins (97.1%), siderophores (94.3%) and phagocytosis resistance (wabG 48.5%, uge 80% and ycfM 57.1%)]. A total of 10 STs were identified although 40% of them clustered on ST25-CC65, and 17% to ST17. The incidence of KPC-2-producing K. pneumoniae reported by the hospital was 0.290 per 1000 admissions. In summary we described an epidemic scenario of multidrug resistant hypermucoviscous KPC-2 producing ST25 K. pneumoniae in our institution.Fil: Vargas, Juan Martin. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Moreno Mochi, María Paula. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Nuñez, J. M.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; ArgentinaFil: Cáceres, M.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; ArgentinaFil: Mochi, S.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; ArgentinaFil: del Campo Moreno, R.. Instituto Ramón y Cajal de Investigación Sanitaria; EspañaFil: Jure, Maria Angela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; ArgentinaElsevier2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/219138Vargas, Juan Martin; Moreno Mochi, María Paula; Nuñez, J. M.; Cáceres, M.; Mochi, S.; et al.; Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae; Elsevier; Heliyon; 5; 6; 6-2019; 1-62405-8440CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.heliyon.2019.e01829info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:34Zoai:ri.conicet.gov.ar:11336/219138instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:34.564CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae
title Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae
spellingShingle Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae
Vargas, Juan Martin
CARBAPENEMASE
EPIDEMIOLOGY
INFECTIOUS DISEASE
KLEBSIELLA PNEUMONIAE
KPC
MICROBIOLOGY
MULTI-CLONAL
title_short Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae
title_full Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae
title_fullStr Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae
title_full_unstemmed Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae
title_sort Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae
dc.creator.none.fl_str_mv Vargas, Juan Martin
Moreno Mochi, María Paula
Nuñez, J. M.
Cáceres, M.
Mochi, S.
del Campo Moreno, R.
Jure, Maria Angela
author Vargas, Juan Martin
author_facet Vargas, Juan Martin
Moreno Mochi, María Paula
Nuñez, J. M.
Cáceres, M.
Mochi, S.
del Campo Moreno, R.
Jure, Maria Angela
author_role author
author2 Moreno Mochi, María Paula
Nuñez, J. M.
Cáceres, M.
Mochi, S.
del Campo Moreno, R.
Jure, Maria Angela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CARBAPENEMASE
EPIDEMIOLOGY
INFECTIOUS DISEASE
KLEBSIELLA PNEUMONIAE
KPC
MICROBIOLOGY
MULTI-CLONAL
topic CARBAPENEMASE
EPIDEMIOLOGY
INFECTIOUS DISEASE
KLEBSIELLA PNEUMONIAE
KPC
MICROBIOLOGY
MULTI-CLONAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Carbapenemase-producing Klebsiella pneumoniae (CRKP) are increasingly reported worldwide being necessary the local epidemiological monitoring. Our aim was to characterize the hypermucoviscous CRKP isolates collected in our hospital during a 6 months period. Carriage of the carbapenemase genes (blaKPC, blaNDM, blaVIM and blaOXA-48), extended spectrum β-lactamases (blaSHV-2, blaCTX-M) and the virulence genes (magA, k2A, rmpA, wabG, uge, allS, entB, ycfM, kpn, wcaG, fimH, mrkD, iutA, iroN, hly and cnf-1) were determined by multiplex-PCR. Genetic relationship among the isolates was performed by PFGE and MLST. A total of 35 isolates were recovered, being the urinary and respiratory tract the most common infection sites (34.2%). The blaKPC-2 gene was present in all the isolates, coexisting with blaCTX-M-2 (45.7%), blaSHV-2 (28.6%), and blaCTX-M-2/blaSHV-2 (14.3%). The capsular serotype K2 corresponded with 68.6% of the isolates. Virulence factors frequency were variable [adhesins (97.1%), siderophores (94.3%) and phagocytosis resistance (wabG 48.5%, uge 80% and ycfM 57.1%)]. A total of 10 STs were identified although 40% of them clustered on ST25-CC65, and 17% to ST17. The incidence of KPC-2-producing K. pneumoniae reported by the hospital was 0.290 per 1000 admissions. In summary we described an epidemic scenario of multidrug resistant hypermucoviscous KPC-2 producing ST25 K. pneumoniae in our institution.
Fil: Vargas, Juan Martin. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Moreno Mochi, María Paula. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Nuñez, J. M.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; Argentina
Fil: Cáceres, M.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; Argentina
Fil: Mochi, S.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; Argentina
Fil: del Campo Moreno, R.. Instituto Ramón y Cajal de Investigación Sanitaria; España
Fil: Jure, Maria Angela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología "Luis Verna". Cátedra de Bacteriología; Argentina
description Carbapenemase-producing Klebsiella pneumoniae (CRKP) are increasingly reported worldwide being necessary the local epidemiological monitoring. Our aim was to characterize the hypermucoviscous CRKP isolates collected in our hospital during a 6 months period. Carriage of the carbapenemase genes (blaKPC, blaNDM, blaVIM and blaOXA-48), extended spectrum β-lactamases (blaSHV-2, blaCTX-M) and the virulence genes (magA, k2A, rmpA, wabG, uge, allS, entB, ycfM, kpn, wcaG, fimH, mrkD, iutA, iroN, hly and cnf-1) were determined by multiplex-PCR. Genetic relationship among the isolates was performed by PFGE and MLST. A total of 35 isolates were recovered, being the urinary and respiratory tract the most common infection sites (34.2%). The blaKPC-2 gene was present in all the isolates, coexisting with blaCTX-M-2 (45.7%), blaSHV-2 (28.6%), and blaCTX-M-2/blaSHV-2 (14.3%). The capsular serotype K2 corresponded with 68.6% of the isolates. Virulence factors frequency were variable [adhesins (97.1%), siderophores (94.3%) and phagocytosis resistance (wabG 48.5%, uge 80% and ycfM 57.1%)]. A total of 10 STs were identified although 40% of them clustered on ST25-CC65, and 17% to ST17. The incidence of KPC-2-producing K. pneumoniae reported by the hospital was 0.290 per 1000 admissions. In summary we described an epidemic scenario of multidrug resistant hypermucoviscous KPC-2 producing ST25 K. pneumoniae in our institution.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/219138
Vargas, Juan Martin; Moreno Mochi, María Paula; Nuñez, J. M.; Cáceres, M.; Mochi, S.; et al.; Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae; Elsevier; Heliyon; 5; 6; 6-2019; 1-6
2405-8440
CONICET Digital
CONICET
url http://hdl.handle.net/11336/219138
identifier_str_mv Vargas, Juan Martin; Moreno Mochi, María Paula; Nuñez, J. M.; Cáceres, M.; Mochi, S.; et al.; Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae; Elsevier; Heliyon; 5; 6; 6-2019; 1-6
2405-8440
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.heliyon.2019.e01829
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614168291835904
score 13.070432

Publicaciones similares