Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic
- Autores
- Gómez, Sonia Alejandra; Martino, Florencia; Sanz, María Belén; Escalante, Jenny; de Mendieta, Juan Manuel; Lucero, María Celeste; Ceriana, Paola; Pasteran, Fernando; Corso, Alejandra; Ramirez, Maria Soledad; Faccone, Diego Francisco
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The prevalence of carbapenemase-producing Klebsiella pneumoniae increased significantly during COVID-19 in Argentina, rising from 20 % in 2019 to 30 % in 2021. Additionally, there was a notable increase of K. pneumoniae ST307 co-producing KPC and NDM. We aimed to reveal the genetic structure of the plasmids harbored in six isolates of K. pneumoniae ST307 co-producing KPC and NDM and to expression levels of both carbapenemases in a cell. The isolates were collected between November 2020 and September 2021 and were selected according to: (i) diversity of allelic variant combinations: blaKPC-2 plus blaNDM-1 (n = 2), blaKPC-2 plus blaNDM-5 (n = 2), blaKPC-3 plus blaNDM-1 (n = 2); (ii) different hospitals and jurisdictions; and (iii) differences in pulsotype by Xba-I-PFGE. The isolates were studied by whole genome sequencing (Illumina NextSeq 550 and with GridION) and with a transcriptional analysis using quantitative real time-PCR. The isolates carried between two and four plasmids with sizes from 3.7 to 250 Kb. Twenty plasmids with nine replicons were identified including three distinct hybrid replicon combinations. blaKPC-2 was found in three plasmid backbones [IncM1, IncFIB(pQil)/IncFII(K), and IncFIB/H1B] while the rest were associated with a single plasmid type: blaNDM-1 (IncC), blaNDM-5 (IncFIB/H1B) and blaKPC-3 (IncR). The basal expression level of KPC-2 was statistically higher than that of NDM-1/5. Our results show that the dissemination of NDM and KPC in K. pneumoniae ST307 was driven through diverse plasmids, highlighting the capacity of this high-risk clone to acquire, maintain and spread multiple carbapenemases.
Fil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Sanz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Escalante, Jenny. California State University; Estados Unidos
Fil: de Mendieta, Juan Manuel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Lucero, María Celeste. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Ceriana, Paola. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Corso, Alejandra. California State University; Estados Unidos
Fil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Faccone, Diego Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina - Materia
-
KLEBSIELLA PNEUMONIAE
ST307
KPC
NDM - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/272014
Ver los metadatos del registro completo
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Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemicGómez, Sonia AlejandraMartino, FlorenciaSanz, María BelénEscalante, Jennyde Mendieta, Juan ManuelLucero, María CelesteCeriana, PaolaPasteran, FernandoCorso, AlejandraRamirez, Maria SoledadFaccone, Diego FranciscoKLEBSIELLA PNEUMONIAEST307KPCNDMhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The prevalence of carbapenemase-producing Klebsiella pneumoniae increased significantly during COVID-19 in Argentina, rising from 20 % in 2019 to 30 % in 2021. Additionally, there was a notable increase of K. pneumoniae ST307 co-producing KPC and NDM. We aimed to reveal the genetic structure of the plasmids harbored in six isolates of K. pneumoniae ST307 co-producing KPC and NDM and to expression levels of both carbapenemases in a cell. The isolates were collected between November 2020 and September 2021 and were selected according to: (i) diversity of allelic variant combinations: blaKPC-2 plus blaNDM-1 (n = 2), blaKPC-2 plus blaNDM-5 (n = 2), blaKPC-3 plus blaNDM-1 (n = 2); (ii) different hospitals and jurisdictions; and (iii) differences in pulsotype by Xba-I-PFGE. The isolates were studied by whole genome sequencing (Illumina NextSeq 550 and with GridION) and with a transcriptional analysis using quantitative real time-PCR. The isolates carried between two and four plasmids with sizes from 3.7 to 250 Kb. Twenty plasmids with nine replicons were identified including three distinct hybrid replicon combinations. blaKPC-2 was found in three plasmid backbones [IncM1, IncFIB(pQil)/IncFII(K), and IncFIB/H1B] while the rest were associated with a single plasmid type: blaNDM-1 (IncC), blaNDM-5 (IncFIB/H1B) and blaKPC-3 (IncR). The basal expression level of KPC-2 was statistically higher than that of NDM-1/5. Our results show that the dissemination of NDM and KPC in K. pneumoniae ST307 was driven through diverse plasmids, highlighting the capacity of this high-risk clone to acquire, maintain and spread multiple carbapenemases.Fil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Sanz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Escalante, Jenny. California State University; Estados UnidosFil: de Mendieta, Juan Manuel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Lucero, María Celeste. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Ceriana, Paola. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Corso, Alejandra. California State University; Estados UnidosFil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Faccone, Diego Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaElsevier Science2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272014Gómez, Sonia Alejandra; Martino, Florencia; Sanz, María Belén; Escalante, Jenny; de Mendieta, Juan Manuel; et al.; Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic; Elsevier Science; Infection, Genetics and Evolution; 133; 9-2025; 1-91567-1348CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S156713482500084Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2025.105795info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:39:09Zoai:ri.conicet.gov.ar:11336/272014instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:39:09.651CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic |
title |
Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic |
spellingShingle |
Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic Gómez, Sonia Alejandra KLEBSIELLA PNEUMONIAE ST307 KPC NDM |
title_short |
Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic |
title_full |
Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic |
title_fullStr |
Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic |
title_full_unstemmed |
Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic |
title_sort |
Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic |
dc.creator.none.fl_str_mv |
Gómez, Sonia Alejandra Martino, Florencia Sanz, María Belén Escalante, Jenny de Mendieta, Juan Manuel Lucero, María Celeste Ceriana, Paola Pasteran, Fernando Corso, Alejandra Ramirez, Maria Soledad Faccone, Diego Francisco |
author |
Gómez, Sonia Alejandra |
author_facet |
Gómez, Sonia Alejandra Martino, Florencia Sanz, María Belén Escalante, Jenny de Mendieta, Juan Manuel Lucero, María Celeste Ceriana, Paola Pasteran, Fernando Corso, Alejandra Ramirez, Maria Soledad Faccone, Diego Francisco |
author_role |
author |
author2 |
Martino, Florencia Sanz, María Belén Escalante, Jenny de Mendieta, Juan Manuel Lucero, María Celeste Ceriana, Paola Pasteran, Fernando Corso, Alejandra Ramirez, Maria Soledad Faccone, Diego Francisco |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
KLEBSIELLA PNEUMONIAE ST307 KPC NDM |
topic |
KLEBSIELLA PNEUMONIAE ST307 KPC NDM |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The prevalence of carbapenemase-producing Klebsiella pneumoniae increased significantly during COVID-19 in Argentina, rising from 20 % in 2019 to 30 % in 2021. Additionally, there was a notable increase of K. pneumoniae ST307 co-producing KPC and NDM. We aimed to reveal the genetic structure of the plasmids harbored in six isolates of K. pneumoniae ST307 co-producing KPC and NDM and to expression levels of both carbapenemases in a cell. The isolates were collected between November 2020 and September 2021 and were selected according to: (i) diversity of allelic variant combinations: blaKPC-2 plus blaNDM-1 (n = 2), blaKPC-2 plus blaNDM-5 (n = 2), blaKPC-3 plus blaNDM-1 (n = 2); (ii) different hospitals and jurisdictions; and (iii) differences in pulsotype by Xba-I-PFGE. The isolates were studied by whole genome sequencing (Illumina NextSeq 550 and with GridION) and with a transcriptional analysis using quantitative real time-PCR. The isolates carried between two and four plasmids with sizes from 3.7 to 250 Kb. Twenty plasmids with nine replicons were identified including three distinct hybrid replicon combinations. blaKPC-2 was found in three plasmid backbones [IncM1, IncFIB(pQil)/IncFII(K), and IncFIB/H1B] while the rest were associated with a single plasmid type: blaNDM-1 (IncC), blaNDM-5 (IncFIB/H1B) and blaKPC-3 (IncR). The basal expression level of KPC-2 was statistically higher than that of NDM-1/5. Our results show that the dissemination of NDM and KPC in K. pneumoniae ST307 was driven through diverse plasmids, highlighting the capacity of this high-risk clone to acquire, maintain and spread multiple carbapenemases. Fil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina Fil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina Fil: Sanz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina Fil: Escalante, Jenny. California State University; Estados Unidos Fil: de Mendieta, Juan Manuel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina Fil: Lucero, María Celeste. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina Fil: Ceriana, Paola. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina Fil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina Fil: Corso, Alejandra. California State University; Estados Unidos Fil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina Fil: Faccone, Diego Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina |
description |
The prevalence of carbapenemase-producing Klebsiella pneumoniae increased significantly during COVID-19 in Argentina, rising from 20 % in 2019 to 30 % in 2021. Additionally, there was a notable increase of K. pneumoniae ST307 co-producing KPC and NDM. We aimed to reveal the genetic structure of the plasmids harbored in six isolates of K. pneumoniae ST307 co-producing KPC and NDM and to expression levels of both carbapenemases in a cell. The isolates were collected between November 2020 and September 2021 and were selected according to: (i) diversity of allelic variant combinations: blaKPC-2 plus blaNDM-1 (n = 2), blaKPC-2 plus blaNDM-5 (n = 2), blaKPC-3 plus blaNDM-1 (n = 2); (ii) different hospitals and jurisdictions; and (iii) differences in pulsotype by Xba-I-PFGE. The isolates were studied by whole genome sequencing (Illumina NextSeq 550 and with GridION) and with a transcriptional analysis using quantitative real time-PCR. The isolates carried between two and four plasmids with sizes from 3.7 to 250 Kb. Twenty plasmids with nine replicons were identified including three distinct hybrid replicon combinations. blaKPC-2 was found in three plasmid backbones [IncM1, IncFIB(pQil)/IncFII(K), and IncFIB/H1B] while the rest were associated with a single plasmid type: blaNDM-1 (IncC), blaNDM-5 (IncFIB/H1B) and blaKPC-3 (IncR). The basal expression level of KPC-2 was statistically higher than that of NDM-1/5. Our results show that the dissemination of NDM and KPC in K. pneumoniae ST307 was driven through diverse plasmids, highlighting the capacity of this high-risk clone to acquire, maintain and spread multiple carbapenemases. |
publishDate |
2025 |
dc.date.none.fl_str_mv |
2025-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/272014 Gómez, Sonia Alejandra; Martino, Florencia; Sanz, María Belén; Escalante, Jenny; de Mendieta, Juan Manuel; et al.; Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic; Elsevier Science; Infection, Genetics and Evolution; 133; 9-2025; 1-9 1567-1348 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/272014 |
identifier_str_mv |
Gómez, Sonia Alejandra; Martino, Florencia; Sanz, María Belén; Escalante, Jenny; de Mendieta, Juan Manuel; et al.; Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic; Elsevier Science; Infection, Genetics and Evolution; 133; 9-2025; 1-9 1567-1348 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S156713482500084X info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2025.105795 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
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application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |