Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic

Autores
Gómez, Sonia Alejandra; Martino, Florencia; Sanz, María Belén; Escalante, Jenny; de Mendieta, Juan Manuel; Lucero, María Celeste; Ceriana, Paola; Pasteran, Fernando; Corso, Alejandra; Ramirez, Maria Soledad; Faccone, Diego Francisco
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The prevalence of carbapenemase-producing Klebsiella pneumoniae increased significantly during COVID-19 in Argentina, rising from 20 % in 2019 to 30 % in 2021. Additionally, there was a notable increase of K. pneumoniae ST307 co-producing KPC and NDM. We aimed to reveal the genetic structure of the plasmids harbored in six isolates of K. pneumoniae ST307 co-producing KPC and NDM and to expression levels of both carbapenemases in a cell. The isolates were collected between November 2020 and September 2021 and were selected according to: (i) diversity of allelic variant combinations: blaKPC-2 plus blaNDM-1 (n = 2), blaKPC-2 plus blaNDM-5 (n = 2), blaKPC-3 plus blaNDM-1 (n = 2); (ii) different hospitals and jurisdictions; and (iii) differences in pulsotype by Xba-I-PFGE. The isolates were studied by whole genome sequencing (Illumina NextSeq 550 and with GridION) and with a transcriptional analysis using quantitative real time-PCR. The isolates carried between two and four plasmids with sizes from 3.7 to 250 Kb. Twenty plasmids with nine replicons were identified including three distinct hybrid replicon combinations. blaKPC-2 was found in three plasmid backbones [IncM1, IncFIB(pQil)/IncFII(K), and IncFIB/H1B] while the rest were associated with a single plasmid type: blaNDM-1 (IncC), blaNDM-5 (IncFIB/H1B) and blaKPC-3 (IncR). The basal expression level of KPC-2 was statistically higher than that of NDM-1/5. Our results show that the dissemination of NDM and KPC in K. pneumoniae ST307 was driven through diverse plasmids, highlighting the capacity of this high-risk clone to acquire, maintain and spread multiple carbapenemases.
Fil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Sanz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Escalante, Jenny. California State University; Estados Unidos
Fil: de Mendieta, Juan Manuel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Lucero, María Celeste. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Ceriana, Paola. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Corso, Alejandra. California State University; Estados Unidos
Fil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Faccone, Diego Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Materia
KLEBSIELLA PNEUMONIAE
ST307
KPC
NDM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272014

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network_name_str CONICET Digital (CONICET)
spelling Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemicGómez, Sonia AlejandraMartino, FlorenciaSanz, María BelénEscalante, Jennyde Mendieta, Juan ManuelLucero, María CelesteCeriana, PaolaPasteran, FernandoCorso, AlejandraRamirez, Maria SoledadFaccone, Diego FranciscoKLEBSIELLA PNEUMONIAEST307KPCNDMhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The prevalence of carbapenemase-producing Klebsiella pneumoniae increased significantly during COVID-19 in Argentina, rising from 20 % in 2019 to 30 % in 2021. Additionally, there was a notable increase of K. pneumoniae ST307 co-producing KPC and NDM. We aimed to reveal the genetic structure of the plasmids harbored in six isolates of K. pneumoniae ST307 co-producing KPC and NDM and to expression levels of both carbapenemases in a cell. The isolates were collected between November 2020 and September 2021 and were selected according to: (i) diversity of allelic variant combinations: blaKPC-2 plus blaNDM-1 (n = 2), blaKPC-2 plus blaNDM-5 (n = 2), blaKPC-3 plus blaNDM-1 (n = 2); (ii) different hospitals and jurisdictions; and (iii) differences in pulsotype by Xba-I-PFGE. The isolates were studied by whole genome sequencing (Illumina NextSeq 550 and with GridION) and with a transcriptional analysis using quantitative real time-PCR. The isolates carried between two and four plasmids with sizes from 3.7 to 250 Kb. Twenty plasmids with nine replicons were identified including three distinct hybrid replicon combinations. blaKPC-2 was found in three plasmid backbones [IncM1, IncFIB(pQil)/IncFII(K), and IncFIB/H1B] while the rest were associated with a single plasmid type: blaNDM-1 (IncC), blaNDM-5 (IncFIB/H1B) and blaKPC-3 (IncR). The basal expression level of KPC-2 was statistically higher than that of NDM-1/5. Our results show that the dissemination of NDM and KPC in K. pneumoniae ST307 was driven through diverse plasmids, highlighting the capacity of this high-risk clone to acquire, maintain and spread multiple carbapenemases.Fil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Sanz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Escalante, Jenny. California State University; Estados UnidosFil: de Mendieta, Juan Manuel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Lucero, María Celeste. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Ceriana, Paola. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Corso, Alejandra. California State University; Estados UnidosFil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Faccone, Diego Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaElsevier Science2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272014Gómez, Sonia Alejandra; Martino, Florencia; Sanz, María Belén; Escalante, Jenny; de Mendieta, Juan Manuel; et al.; Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic; Elsevier Science; Infection, Genetics and Evolution; 133; 9-2025; 1-91567-1348CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S156713482500084Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2025.105795info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:39:09Zoai:ri.conicet.gov.ar:11336/272014instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:39:09.651CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic
title Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic
spellingShingle Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic
Gómez, Sonia Alejandra
KLEBSIELLA PNEUMONIAE
ST307
KPC
NDM
title_short Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic
title_full Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic
title_fullStr Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic
title_full_unstemmed Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic
title_sort Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic
dc.creator.none.fl_str_mv Gómez, Sonia Alejandra
Martino, Florencia
Sanz, María Belén
Escalante, Jenny
de Mendieta, Juan Manuel
Lucero, María Celeste
Ceriana, Paola
Pasteran, Fernando
Corso, Alejandra
Ramirez, Maria Soledad
Faccone, Diego Francisco
author Gómez, Sonia Alejandra
author_facet Gómez, Sonia Alejandra
Martino, Florencia
Sanz, María Belén
Escalante, Jenny
de Mendieta, Juan Manuel
Lucero, María Celeste
Ceriana, Paola
Pasteran, Fernando
Corso, Alejandra
Ramirez, Maria Soledad
Faccone, Diego Francisco
author_role author
author2 Martino, Florencia
Sanz, María Belén
Escalante, Jenny
de Mendieta, Juan Manuel
Lucero, María Celeste
Ceriana, Paola
Pasteran, Fernando
Corso, Alejandra
Ramirez, Maria Soledad
Faccone, Diego Francisco
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv KLEBSIELLA PNEUMONIAE
ST307
KPC
NDM
topic KLEBSIELLA PNEUMONIAE
ST307
KPC
NDM
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The prevalence of carbapenemase-producing Klebsiella pneumoniae increased significantly during COVID-19 in Argentina, rising from 20 % in 2019 to 30 % in 2021. Additionally, there was a notable increase of K. pneumoniae ST307 co-producing KPC and NDM. We aimed to reveal the genetic structure of the plasmids harbored in six isolates of K. pneumoniae ST307 co-producing KPC and NDM and to expression levels of both carbapenemases in a cell. The isolates were collected between November 2020 and September 2021 and were selected according to: (i) diversity of allelic variant combinations: blaKPC-2 plus blaNDM-1 (n = 2), blaKPC-2 plus blaNDM-5 (n = 2), blaKPC-3 plus blaNDM-1 (n = 2); (ii) different hospitals and jurisdictions; and (iii) differences in pulsotype by Xba-I-PFGE. The isolates were studied by whole genome sequencing (Illumina NextSeq 550 and with GridION) and with a transcriptional analysis using quantitative real time-PCR. The isolates carried between two and four plasmids with sizes from 3.7 to 250 Kb. Twenty plasmids with nine replicons were identified including three distinct hybrid replicon combinations. blaKPC-2 was found in three plasmid backbones [IncM1, IncFIB(pQil)/IncFII(K), and IncFIB/H1B] while the rest were associated with a single plasmid type: blaNDM-1 (IncC), blaNDM-5 (IncFIB/H1B) and blaKPC-3 (IncR). The basal expression level of KPC-2 was statistically higher than that of NDM-1/5. Our results show that the dissemination of NDM and KPC in K. pneumoniae ST307 was driven through diverse plasmids, highlighting the capacity of this high-risk clone to acquire, maintain and spread multiple carbapenemases.
Fil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Sanz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Escalante, Jenny. California State University; Estados Unidos
Fil: de Mendieta, Juan Manuel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Lucero, María Celeste. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Ceriana, Paola. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Corso, Alejandra. California State University; Estados Unidos
Fil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Faccone, Diego Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
description The prevalence of carbapenemase-producing Klebsiella pneumoniae increased significantly during COVID-19 in Argentina, rising from 20 % in 2019 to 30 % in 2021. Additionally, there was a notable increase of K. pneumoniae ST307 co-producing KPC and NDM. We aimed to reveal the genetic structure of the plasmids harbored in six isolates of K. pneumoniae ST307 co-producing KPC and NDM and to expression levels of both carbapenemases in a cell. The isolates were collected between November 2020 and September 2021 and were selected according to: (i) diversity of allelic variant combinations: blaKPC-2 plus blaNDM-1 (n = 2), blaKPC-2 plus blaNDM-5 (n = 2), blaKPC-3 plus blaNDM-1 (n = 2); (ii) different hospitals and jurisdictions; and (iii) differences in pulsotype by Xba-I-PFGE. The isolates were studied by whole genome sequencing (Illumina NextSeq 550 and with GridION) and with a transcriptional analysis using quantitative real time-PCR. The isolates carried between two and four plasmids with sizes from 3.7 to 250 Kb. Twenty plasmids with nine replicons were identified including three distinct hybrid replicon combinations. blaKPC-2 was found in three plasmid backbones [IncM1, IncFIB(pQil)/IncFII(K), and IncFIB/H1B] while the rest were associated with a single plasmid type: blaNDM-1 (IncC), blaNDM-5 (IncFIB/H1B) and blaKPC-3 (IncR). The basal expression level of KPC-2 was statistically higher than that of NDM-1/5. Our results show that the dissemination of NDM and KPC in K. pneumoniae ST307 was driven through diverse plasmids, highlighting the capacity of this high-risk clone to acquire, maintain and spread multiple carbapenemases.
publishDate 2025
dc.date.none.fl_str_mv 2025-09
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info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272014
Gómez, Sonia Alejandra; Martino, Florencia; Sanz, María Belén; Escalante, Jenny; de Mendieta, Juan Manuel; et al.; Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic; Elsevier Science; Infection, Genetics and Evolution; 133; 9-2025; 1-9
1567-1348
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272014
identifier_str_mv Gómez, Sonia Alejandra; Martino, Florencia; Sanz, María Belén; Escalante, Jenny; de Mendieta, Juan Manuel; et al.; Plasmid diversity in Klebsiella pneumoniae ST307 co-producing KPC plus NDM recovered during the COVID-19 pandemic; Elsevier Science; Infection, Genetics and Evolution; 133; 9-2025; 1-9
1567-1348
CONICET Digital
CONICET
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language eng
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