Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes

Autores
Cela, Eliana Maiten; Weill, Federico Simon; Paz, Mariela Laura; Leoni, Juliana; Gonzalez Maglio, Daniel Horacio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present work, we aimed to evaluate how UVR exposure could affect keratinocytes antimicrobial inflammatory response to gram-positive and gramnegative bacteria components, such as LTA and LPS. We observed that UVR increased keratinocytes responsiveness to microbial external components. Interestingly, this response was more intense to LTA than to LPS, in contrast to the response commonly observed in other cell types like macrophages, where the immune response is bias towards gram-negative bacteria. This increase in the response to microbial components, especially to LTA, was only partially observed in VitD3- treated cells showing that other molecules might also be involved in this response. The preferential recognition of gram-positive bacteria can be considered as a specialization of the epithelium to its microflora. If this epithelium suffers an injury (like exposure to harmful radiation), it has to modify its response to microorganisms in order to avoid their entry into sites where they might produce infections. In this context, the increment in the inflammatory response observed in UVR-irradiated LTA challenged keratinocytes is a novel evidence to understand the complex balance between the skin, its microflora and the environment.
Fil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Weill, Federico S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Paz, Mariela Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gonzalez Maglio, Daniel Horacio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Microbiologia,inmunologia y Biotecnolog.. Catedra de Inmunologia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
INFLAMMATION
EXTERNAL BARRIER
MICROFLORA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8038
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8038
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytesCela, Eliana MaitenWeill, Federico SimonPaz, Mariela LauraLeoni, JulianaGonzalez Maglio, Daniel HoracioINFLAMMATIONEXTERNAL BARRIERMICROFLORAhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In the present work, we aimed to evaluate how UVR exposure could affect keratinocytes antimicrobial inflammatory response to gram-positive and gramnegative bacteria components, such as LTA and LPS. We observed that UVR increased keratinocytes responsiveness to microbial external components. Interestingly, this response was more intense to LTA than to LPS, in contrast to the response commonly observed in other cell types like macrophages, where the immune response is bias towards gram-negative bacteria. This increase in the response to microbial components, especially to LTA, was only partially observed in VitD3- treated cells showing that other molecules might also be involved in this response. The preferential recognition of gram-positive bacteria can be considered as a specialization of the epithelium to its microflora. If this epithelium suffers an injury (like exposure to harmful radiation), it has to modify its response to microorganisms in order to avoid their entry into sites where they might produce infections. In this context, the increment in the inflammatory response observed in UVR-irradiated LTA challenged keratinocytes is a novel evidence to understand the complex balance between the skin, its microflora and the environment.Fil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Weill, Federico S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Paz, Mariela Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Gonzalez Maglio, Daniel Horacio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Microbiologia,inmunologia y Biotecnolog.. Catedra de Inmunologia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaWiley Blackwell Publishing, Inc2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8038Cela, Eliana Maiten; Weill, Federico Simon; Paz, Mariela Laura; Leoni, Juliana; Gonzalez Maglio, Daniel Horacio; Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes; Wiley Blackwell Publishing, Inc; Photodermatology, Photoimmunology & Photomedicine; 31; 2; 3-2015; 111-1140905-4383enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/phpp.12149/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/phpp.12149info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:53:36Zoai:ri.conicet.gov.ar:11336/8038instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:53:36.328CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes
title Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes
spellingShingle Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes
Cela, Eliana Maiten
INFLAMMATION
EXTERNAL BARRIER
MICROFLORA
title_short Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes
title_full Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes
title_fullStr Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes
title_full_unstemmed Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes
title_sort Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes
dc.creator.none.fl_str_mv Cela, Eliana Maiten
Weill, Federico Simon
Paz, Mariela Laura
Leoni, Juliana
Gonzalez Maglio, Daniel Horacio
author Cela, Eliana Maiten
author_facet Cela, Eliana Maiten
Weill, Federico Simon
Paz, Mariela Laura
Leoni, Juliana
Gonzalez Maglio, Daniel Horacio
author_role author
author2 Weill, Federico Simon
Paz, Mariela Laura
Leoni, Juliana
Gonzalez Maglio, Daniel Horacio
author2_role author
author
author
author
dc.subject.none.fl_str_mv INFLAMMATION
EXTERNAL BARRIER
MICROFLORA
topic INFLAMMATION
EXTERNAL BARRIER
MICROFLORA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In the present work, we aimed to evaluate how UVR exposure could affect keratinocytes antimicrobial inflammatory response to gram-positive and gramnegative bacteria components, such as LTA and LPS. We observed that UVR increased keratinocytes responsiveness to microbial external components. Interestingly, this response was more intense to LTA than to LPS, in contrast to the response commonly observed in other cell types like macrophages, where the immune response is bias towards gram-negative bacteria. This increase in the response to microbial components, especially to LTA, was only partially observed in VitD3- treated cells showing that other molecules might also be involved in this response. The preferential recognition of gram-positive bacteria can be considered as a specialization of the epithelium to its microflora. If this epithelium suffers an injury (like exposure to harmful radiation), it has to modify its response to microorganisms in order to avoid their entry into sites where they might produce infections. In this context, the increment in the inflammatory response observed in UVR-irradiated LTA challenged keratinocytes is a novel evidence to understand the complex balance between the skin, its microflora and the environment.
Fil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Weill, Federico S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Paz, Mariela Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gonzalez Maglio, Daniel Horacio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Microbiologia,inmunologia y Biotecnolog.. Catedra de Inmunologia; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description In the present work, we aimed to evaluate how UVR exposure could affect keratinocytes antimicrobial inflammatory response to gram-positive and gramnegative bacteria components, such as LTA and LPS. We observed that UVR increased keratinocytes responsiveness to microbial external components. Interestingly, this response was more intense to LTA than to LPS, in contrast to the response commonly observed in other cell types like macrophages, where the immune response is bias towards gram-negative bacteria. This increase in the response to microbial components, especially to LTA, was only partially observed in VitD3- treated cells showing that other molecules might also be involved in this response. The preferential recognition of gram-positive bacteria can be considered as a specialization of the epithelium to its microflora. If this epithelium suffers an injury (like exposure to harmful radiation), it has to modify its response to microorganisms in order to avoid their entry into sites where they might produce infections. In this context, the increment in the inflammatory response observed in UVR-irradiated LTA challenged keratinocytes is a novel evidence to understand the complex balance between the skin, its microflora and the environment.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8038
Cela, Eliana Maiten; Weill, Federico Simon; Paz, Mariela Laura; Leoni, Juliana; Gonzalez Maglio, Daniel Horacio; Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes; Wiley Blackwell Publishing, Inc; Photodermatology, Photoimmunology & Photomedicine; 31; 2; 3-2015; 111-114
0905-4383
url http://hdl.handle.net/11336/8038
identifier_str_mv Cela, Eliana Maiten; Weill, Federico Simon; Paz, Mariela Laura; Leoni, Juliana; Gonzalez Maglio, Daniel Horacio; Lipoteichoic acid challenge induces higher inflammatory responses than lipopolysaccharide in UV irradiated keratinocytes; Wiley Blackwell Publishing, Inc; Photodermatology, Photoimmunology & Photomedicine; 31; 2; 3-2015; 111-114
0905-4383
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/phpp.12149/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/phpp.12149
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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