Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposur...

Autores
Cela, Eliana Maiten; Friedrich, Adrián; Paz, Mariela Laura; Vanzulli, Silvia; Leoni, Juliana; Gonzalez Maglio, Daniel Horacio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The modulatory effects of solar ultraviolet radiation (UVR) on the immune system have been widely studied. As the skin is the main target of UVR, our purpose was to compare the impact of two contrasting ways to be exposed to sunlight on the skin innate immunity. Hairless mice were UV irradiated with a single high UV dose (shUVd) simulating a harmful exposure, or with repetitive low UV doses (rlUVd) simulating short occasional daily exposures. Skin samples were taken at different times post-UV irradiation to evaluate skin histology, inflammatory cell recruitment, epidermal T cell population and the mitochondrial function of epidermal cells. The transcriptional profiles of pro-inflammatory cytokines, chemokines, antimicrobial peptides and TLRs were evaluated by RT-PCR and ELISA in tissue homogenates. Finally, a lymphangiography was performed to assess modification in the lymphatic vessel system. A shUVd produces a deep inflammatory state characterized by the production of pro-inflammatory cytokines and chemokines that, in turn, induces the recruitment of neutrophils and macrophages into the irradiated area. On the other hand, rlUVd drive the skin to a photo-induced alert state in which there is no sign of inflammation, but the epithelium undergoes changes in thickness, the lymphatic circulation increases, and the transcription of antimicrobial peptides is induced.
Fil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Friedrich, Adrián. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Paz, Mariela Laura. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Gonzalez Maglio, Daniel Horacio. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Materia
Inflammation
Skin
Ultraviolet Radiation
Photoadaptation
Chemotaxis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8044
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8044
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposuresCela, Eliana MaitenFriedrich, AdriánPaz, Mariela LauraVanzulli, SilviaLeoni, JulianaGonzalez Maglio, Daniel HoracioInflammationSkinUltraviolet RadiationPhotoadaptationChemotaxishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The modulatory effects of solar ultraviolet radiation (UVR) on the immune system have been widely studied. As the skin is the main target of UVR, our purpose was to compare the impact of two contrasting ways to be exposed to sunlight on the skin innate immunity. Hairless mice were UV irradiated with a single high UV dose (shUVd) simulating a harmful exposure, or with repetitive low UV doses (rlUVd) simulating short occasional daily exposures. Skin samples were taken at different times post-UV irradiation to evaluate skin histology, inflammatory cell recruitment, epidermal T cell population and the mitochondrial function of epidermal cells. The transcriptional profiles of pro-inflammatory cytokines, chemokines, antimicrobial peptides and TLRs were evaluated by RT-PCR and ELISA in tissue homogenates. Finally, a lymphangiography was performed to assess modification in the lymphatic vessel system. A shUVd produces a deep inflammatory state characterized by the production of pro-inflammatory cytokines and chemokines that, in turn, induces the recruitment of neutrophils and macrophages into the irradiated area. On the other hand, rlUVd drive the skin to a photo-induced alert state in which there is no sign of inflammation, but the epithelium undergoes changes in thickness, the lymphatic circulation increases, and the transcription of antimicrobial peptides is induced.Fil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: Friedrich, Adrián. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: Paz, Mariela Laura. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: Gonzalez Maglio, Daniel Horacio. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaWiley2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8044Cela, Eliana Maiten; Friedrich, Adrián; Paz, Mariela Laura; Vanzulli, Silvia; Leoni, Juliana; et al.; Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposures; Wiley; Immunology; 1; 145; 11-2014; 82-930019-2805enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/imm.12427/abstract;jsessionid=802221F2556ED4E44B7753A243360490.f02t02info:eu-repo/semantics/altIdentifier/doi/10.1111/imm.12427info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4405326/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:23Zoai:ri.conicet.gov.ar:11336/8044instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:23.509CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposures
title Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposures
spellingShingle Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposures
Cela, Eliana Maiten
Inflammation
Skin
Ultraviolet Radiation
Photoadaptation
Chemotaxis
title_short Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposures
title_full Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposures
title_fullStr Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposures
title_full_unstemmed Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposures
title_sort Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposures
dc.creator.none.fl_str_mv Cela, Eliana Maiten
Friedrich, Adrián
Paz, Mariela Laura
Vanzulli, Silvia
Leoni, Juliana
Gonzalez Maglio, Daniel Horacio
author Cela, Eliana Maiten
author_facet Cela, Eliana Maiten
Friedrich, Adrián
Paz, Mariela Laura
Vanzulli, Silvia
Leoni, Juliana
Gonzalez Maglio, Daniel Horacio
author_role author
author2 Friedrich, Adrián
Paz, Mariela Laura
Vanzulli, Silvia
Leoni, Juliana
Gonzalez Maglio, Daniel Horacio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Inflammation
Skin
Ultraviolet Radiation
Photoadaptation
Chemotaxis
topic Inflammation
Skin
Ultraviolet Radiation
Photoadaptation
Chemotaxis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The modulatory effects of solar ultraviolet radiation (UVR) on the immune system have been widely studied. As the skin is the main target of UVR, our purpose was to compare the impact of two contrasting ways to be exposed to sunlight on the skin innate immunity. Hairless mice were UV irradiated with a single high UV dose (shUVd) simulating a harmful exposure, or with repetitive low UV doses (rlUVd) simulating short occasional daily exposures. Skin samples were taken at different times post-UV irradiation to evaluate skin histology, inflammatory cell recruitment, epidermal T cell population and the mitochondrial function of epidermal cells. The transcriptional profiles of pro-inflammatory cytokines, chemokines, antimicrobial peptides and TLRs were evaluated by RT-PCR and ELISA in tissue homogenates. Finally, a lymphangiography was performed to assess modification in the lymphatic vessel system. A shUVd produces a deep inflammatory state characterized by the production of pro-inflammatory cytokines and chemokines that, in turn, induces the recruitment of neutrophils and macrophages into the irradiated area. On the other hand, rlUVd drive the skin to a photo-induced alert state in which there is no sign of inflammation, but the epithelium undergoes changes in thickness, the lymphatic circulation increases, and the transcription of antimicrobial peptides is induced.
Fil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Friedrich, Adrián. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Paz, Mariela Laura. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Gonzalez Maglio, Daniel Horacio. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
description The modulatory effects of solar ultraviolet radiation (UVR) on the immune system have been widely studied. As the skin is the main target of UVR, our purpose was to compare the impact of two contrasting ways to be exposed to sunlight on the skin innate immunity. Hairless mice were UV irradiated with a single high UV dose (shUVd) simulating a harmful exposure, or with repetitive low UV doses (rlUVd) simulating short occasional daily exposures. Skin samples were taken at different times post-UV irradiation to evaluate skin histology, inflammatory cell recruitment, epidermal T cell population and the mitochondrial function of epidermal cells. The transcriptional profiles of pro-inflammatory cytokines, chemokines, antimicrobial peptides and TLRs were evaluated by RT-PCR and ELISA in tissue homogenates. Finally, a lymphangiography was performed to assess modification in the lymphatic vessel system. A shUVd produces a deep inflammatory state characterized by the production of pro-inflammatory cytokines and chemokines that, in turn, induces the recruitment of neutrophils and macrophages into the irradiated area. On the other hand, rlUVd drive the skin to a photo-induced alert state in which there is no sign of inflammation, but the epithelium undergoes changes in thickness, the lymphatic circulation increases, and the transcription of antimicrobial peptides is induced.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8044
Cela, Eliana Maiten; Friedrich, Adrián; Paz, Mariela Laura; Vanzulli, Silvia; Leoni, Juliana; et al.; Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposures; Wiley; Immunology; 1; 145; 11-2014; 82-93
0019-2805
url http://hdl.handle.net/11336/8044
identifier_str_mv Cela, Eliana Maiten; Friedrich, Adrián; Paz, Mariela Laura; Vanzulli, Silvia; Leoni, Juliana; et al.; Time-course study of different innate immune mediators produced by ultraviolet irradiated skin. Comparative effects between short and daily versus harmful and imprudent UV- exposures; Wiley; Immunology; 1; 145; 11-2014; 82-93
0019-2805
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/imm.12427/abstract;jsessionid=802221F2556ED4E44B7753A243360490.f02t02
info:eu-repo/semantics/altIdentifier/doi/10.1111/imm.12427
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4405326/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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