Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa

Autores
Buffone, Mariano Gabriel; Calamera, Juan C.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; Calamera, María M.; Storey, Bayard T; Doncel, Gustavo F.; Alvarez, Juan G.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective To investigate the correlation between sperm superoxide dismutase (SOD) content and motility recovery after thawing of cryopreserved human sperm, based on the rationale that this antioxidant enzyme provides protection against reactive oxygen species-induced damage during cryopreservation. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Forty-two consenting normozoospermic patients consulting for infertility. Intervention(s) The SOD content was measured in sperm from unfractionated samples and in sperm recovered from the pellet fraction obtained after discontinuous density gradient centrifugation. Main Outcome Measure(s) Sperm motility was evaluated post-thaw in the two sets of samples and motility recovery was plotted against the sperm SOD content to determine their correlation. Result(s) There was a significant positive correlation between motility recovery after thawing and SOD content in sperm from the 90% gradient pellet containing highly purified mature sperm. There was also a significant negative correlation between motility after thawing and SOD content in the unfractionated sample. Conclusion(s) The positive correlation between post-thaw motility recovery and SOD content in mature spermatozoa provides a good predictor of post-thaw motility recovery after cryopreservation.
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Calamera, Juan C.. Laboratorio de Estudios en Reproducción; Argentina
Fil: Brugo Olmedo, Santiago. Centro Médico Seremas; Argentina
Fil: de Vincentiis, Sabrina. Centro Médico Seremas; Argentina
Fil: Calamera, María M.. Laboratorio de Estudios en Reproducción; Argentina
Fil: Storey, Bayard T. University of Pennsylvania; Estados Unidos
Fil: Doncel, Gustavo F.. Eastern Virginia Medical School. Jones Institute For Reproductive Medicine; Estados Unidos
Fil: Alvarez, Juan G.. Centro ANDROGEN; España. Harvard Medical School; Estados Unidos
Materia
Cryopreservation
Sperm
Sod
Ros
Sperm Motility
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8773
Acceder
id CONICETDig_9e9cf29ea2902bdab37db8f8242a5a94
oai_identifier_str oai:ri.conicet.gov.ar:11336/8773
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoaBuffone, Mariano GabrielCalamera, Juan C.Brugo Olmedo, Santiagode Vincentiis, SabrinaCalamera, María M.Storey, Bayard TDoncel, Gustavo F.Alvarez, Juan G.CryopreservationSpermSodRosSperm Motilityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective To investigate the correlation between sperm superoxide dismutase (SOD) content and motility recovery after thawing of cryopreserved human sperm, based on the rationale that this antioxidant enzyme provides protection against reactive oxygen species-induced damage during cryopreservation. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Forty-two consenting normozoospermic patients consulting for infertility. Intervention(s) The SOD content was measured in sperm from unfractionated samples and in sperm recovered from the pellet fraction obtained after discontinuous density gradient centrifugation. Main Outcome Measure(s) Sperm motility was evaluated post-thaw in the two sets of samples and motility recovery was plotted against the sperm SOD content to determine their correlation. Result(s) There was a significant positive correlation between motility recovery after thawing and SOD content in sperm from the 90% gradient pellet containing highly purified mature sperm. There was also a significant negative correlation between motility after thawing and SOD content in the unfractionated sample. Conclusion(s) The positive correlation between post-thaw motility recovery and SOD content in mature spermatozoa provides a good predictor of post-thaw motility recovery after cryopreservation.Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Calamera, Juan C.. Laboratorio de Estudios en Reproducción; ArgentinaFil: Brugo Olmedo, Santiago. Centro Médico Seremas; ArgentinaFil: de Vincentiis, Sabrina. Centro Médico Seremas; ArgentinaFil: Calamera, María M.. Laboratorio de Estudios en Reproducción; ArgentinaFil: Storey, Bayard T. University of Pennsylvania; Estados UnidosFil: Doncel, Gustavo F.. Eastern Virginia Medical School. Jones Institute For Reproductive Medicine; Estados UnidosFil: Alvarez, Juan G.. Centro ANDROGEN; España. Harvard Medical School; Estados UnidosElsevier2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8773Buffone, Mariano Gabriel; Calamera, Juan C.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; Calamera, María M.; et al.; Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa; Elsevier; Fertility And Sterility; 97; 2; 2-2012; 293-2980015-02821556-5653enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2011.11.012info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S001502821102766Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:01Zoai:ri.conicet.gov.ar:11336/8773instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:01.336CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa
title Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa
spellingShingle Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa
Buffone, Mariano Gabriel
Cryopreservation
Sperm
Sod
Ros
Sperm Motility
title_short Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa
title_full Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa
title_fullStr Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa
title_full_unstemmed Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa
title_sort Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa
dc.creator.none.fl_str_mv Buffone, Mariano Gabriel
Calamera, Juan C.
Brugo Olmedo, Santiago
de Vincentiis, Sabrina
Calamera, María M.
Storey, Bayard T
Doncel, Gustavo F.
Alvarez, Juan G.
author Buffone, Mariano Gabriel
author_facet Buffone, Mariano Gabriel
Calamera, Juan C.
Brugo Olmedo, Santiago
de Vincentiis, Sabrina
Calamera, María M.
Storey, Bayard T
Doncel, Gustavo F.
Alvarez, Juan G.
author_role author
author2 Calamera, Juan C.
Brugo Olmedo, Santiago
de Vincentiis, Sabrina
Calamera, María M.
Storey, Bayard T
Doncel, Gustavo F.
Alvarez, Juan G.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cryopreservation
Sperm
Sod
Ros
Sperm Motility
topic Cryopreservation
Sperm
Sod
Ros
Sperm Motility
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective To investigate the correlation between sperm superoxide dismutase (SOD) content and motility recovery after thawing of cryopreserved human sperm, based on the rationale that this antioxidant enzyme provides protection against reactive oxygen species-induced damage during cryopreservation. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Forty-two consenting normozoospermic patients consulting for infertility. Intervention(s) The SOD content was measured in sperm from unfractionated samples and in sperm recovered from the pellet fraction obtained after discontinuous density gradient centrifugation. Main Outcome Measure(s) Sperm motility was evaluated post-thaw in the two sets of samples and motility recovery was plotted against the sperm SOD content to determine their correlation. Result(s) There was a significant positive correlation between motility recovery after thawing and SOD content in sperm from the 90% gradient pellet containing highly purified mature sperm. There was also a significant negative correlation between motility after thawing and SOD content in the unfractionated sample. Conclusion(s) The positive correlation between post-thaw motility recovery and SOD content in mature spermatozoa provides a good predictor of post-thaw motility recovery after cryopreservation.
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Calamera, Juan C.. Laboratorio de Estudios en Reproducción; Argentina
Fil: Brugo Olmedo, Santiago. Centro Médico Seremas; Argentina
Fil: de Vincentiis, Sabrina. Centro Médico Seremas; Argentina
Fil: Calamera, María M.. Laboratorio de Estudios en Reproducción; Argentina
Fil: Storey, Bayard T. University of Pennsylvania; Estados Unidos
Fil: Doncel, Gustavo F.. Eastern Virginia Medical School. Jones Institute For Reproductive Medicine; Estados Unidos
Fil: Alvarez, Juan G.. Centro ANDROGEN; España. Harvard Medical School; Estados Unidos
description Objective To investigate the correlation between sperm superoxide dismutase (SOD) content and motility recovery after thawing of cryopreserved human sperm, based on the rationale that this antioxidant enzyme provides protection against reactive oxygen species-induced damage during cryopreservation. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Forty-two consenting normozoospermic patients consulting for infertility. Intervention(s) The SOD content was measured in sperm from unfractionated samples and in sperm recovered from the pellet fraction obtained after discontinuous density gradient centrifugation. Main Outcome Measure(s) Sperm motility was evaluated post-thaw in the two sets of samples and motility recovery was plotted against the sperm SOD content to determine their correlation. Result(s) There was a significant positive correlation between motility recovery after thawing and SOD content in sperm from the 90% gradient pellet containing highly purified mature sperm. There was also a significant negative correlation between motility after thawing and SOD content in the unfractionated sample. Conclusion(s) The positive correlation between post-thaw motility recovery and SOD content in mature spermatozoa provides a good predictor of post-thaw motility recovery after cryopreservation.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8773
Buffone, Mariano Gabriel; Calamera, Juan C.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; Calamera, María M.; et al.; Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa; Elsevier; Fertility And Sterility; 97; 2; 2-2012; 293-298
0015-0282
1556-5653
url http://hdl.handle.net/11336/8773
identifier_str_mv Buffone, Mariano Gabriel; Calamera, Juan C.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; Calamera, María M.; et al.; Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa; Elsevier; Fertility And Sterility; 97; 2; 2-2012; 293-298
0015-0282
1556-5653
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2011.11.012
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S001502821102766X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268831451447296
score 13.13397

Publicaciones similares