Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates

Autores
Krapf, Dario; O'brien Echeverría, Emma Dinorah; Maidagan, Paula María; Morales, Enrique Salvador; Visconti, Pablo; Arranz, Cristina Teresa
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Animals with external fertilization, as amphibians, store their sperm in a quiescent state in the testis. When spermatozoa are released into natural fertilization media, the hypotonic shock triggers activation of sperm motility. Rhinella (Bufo) arenarum sperm are immotile in artificial seminal plasma (ASP, resembling testicular plasma tonicity) but acquire in situ flagellar beating upon dilution. However, if components from the egg shelly coat are added to this medium, motility shifts to a progressive pattern. Recently, we have shown that the signal transduction pathway required for in situ motility activation involves a rise in intracellular cAMP through a transmembrane adenylyl cyclase and activation of PKA, mostly in the midpiece and in the sperm head. In this report, we demonstrate that activation of calcineurin (aka PP2B and PPP3) is required for the shift from in situ to progressive sperm motility. The effect of calcineurin is manifested by dephosphorylation of PKC substrates, and can be promoted by intracellular calcium rise by Ca2+ ionophore. Both phosphorylated PKC substrates and calcineurin localized to the flagella, indicating a clear differentiation between compartmentalization of PKA and calcineurin pathways. Moreover, no crosstalk is observed between these signaling events, even though both pathways are required for progressive motility acquisition as discussed.
Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: O'brien Echeverría, Emma Dinorah. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Maidagan, Paula María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Morales, Enrique Salvador. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Visconti, Pablo. University Of Massachussets; Estados Unidos
Fil: Arranz, Cristina Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
Progressive Sperm Motilityogressive Sperm Motility
Amphibian Sperm
Sperm Capacitation
Pkc
Calcineurin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8619
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8619
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC SubstratesKrapf, DarioO'brien Echeverría, Emma DinorahMaidagan, Paula MaríaMorales, Enrique SalvadorVisconti, PabloArranz, Cristina TeresaProgressive Sperm Motilityogressive Sperm MotilityAmphibian SpermSperm CapacitationPkcCalcineurinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Animals with external fertilization, as amphibians, store their sperm in a quiescent state in the testis. When spermatozoa are released into natural fertilization media, the hypotonic shock triggers activation of sperm motility. Rhinella (Bufo) arenarum sperm are immotile in artificial seminal plasma (ASP, resembling testicular plasma tonicity) but acquire in situ flagellar beating upon dilution. However, if components from the egg shelly coat are added to this medium, motility shifts to a progressive pattern. Recently, we have shown that the signal transduction pathway required for in situ motility activation involves a rise in intracellular cAMP through a transmembrane adenylyl cyclase and activation of PKA, mostly in the midpiece and in the sperm head. In this report, we demonstrate that activation of calcineurin (aka PP2B and PPP3) is required for the shift from in situ to progressive sperm motility. The effect of calcineurin is manifested by dephosphorylation of PKC substrates, and can be promoted by intracellular calcium rise by Ca2+ ionophore. Both phosphorylated PKC substrates and calcineurin localized to the flagella, indicating a clear differentiation between compartmentalization of PKA and calcineurin pathways. Moreover, no crosstalk is observed between these signaling events, even though both pathways are required for progressive motility acquisition as discussed.Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: O'brien Echeverría, Emma Dinorah. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Maidagan, Paula María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Morales, Enrique Salvador. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Visconti, Pablo. University Of Massachussets; Estados UnidosFil: Arranz, Cristina Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaWiley2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8619Krapf, Dario; O'brien Echeverría, Emma Dinorah; Maidagan, Paula María; Morales, Enrique Salvador; Visconti, Pablo; et al.; Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates; Wiley; Journal Of Cellular Physiology; 229; 10; 10-2014; 1378-13860021-9541enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jcp.24571info:eu-repo/semantics/altIdentifier/doi/10.1002/jcp.24571info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:56Zoai:ri.conicet.gov.ar:11336/8619instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:57.549CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates
title Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates
spellingShingle Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates
Krapf, Dario
Progressive Sperm Motilityogressive Sperm Motility
Amphibian Sperm
Sperm Capacitation
Pkc
Calcineurin
title_short Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates
title_full Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates
title_fullStr Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates
title_full_unstemmed Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates
title_sort Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates
dc.creator.none.fl_str_mv Krapf, Dario
O'brien Echeverría, Emma Dinorah
Maidagan, Paula María
Morales, Enrique Salvador
Visconti, Pablo
Arranz, Cristina Teresa
author Krapf, Dario
author_facet Krapf, Dario
O'brien Echeverría, Emma Dinorah
Maidagan, Paula María
Morales, Enrique Salvador
Visconti, Pablo
Arranz, Cristina Teresa
author_role author
author2 O'brien Echeverría, Emma Dinorah
Maidagan, Paula María
Morales, Enrique Salvador
Visconti, Pablo
Arranz, Cristina Teresa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Progressive Sperm Motilityogressive Sperm Motility
Amphibian Sperm
Sperm Capacitation
Pkc
Calcineurin
topic Progressive Sperm Motilityogressive Sperm Motility
Amphibian Sperm
Sperm Capacitation
Pkc
Calcineurin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Animals with external fertilization, as amphibians, store their sperm in a quiescent state in the testis. When spermatozoa are released into natural fertilization media, the hypotonic shock triggers activation of sperm motility. Rhinella (Bufo) arenarum sperm are immotile in artificial seminal plasma (ASP, resembling testicular plasma tonicity) but acquire in situ flagellar beating upon dilution. However, if components from the egg shelly coat are added to this medium, motility shifts to a progressive pattern. Recently, we have shown that the signal transduction pathway required for in situ motility activation involves a rise in intracellular cAMP through a transmembrane adenylyl cyclase and activation of PKA, mostly in the midpiece and in the sperm head. In this report, we demonstrate that activation of calcineurin (aka PP2B and PPP3) is required for the shift from in situ to progressive sperm motility. The effect of calcineurin is manifested by dephosphorylation of PKC substrates, and can be promoted by intracellular calcium rise by Ca2+ ionophore. Both phosphorylated PKC substrates and calcineurin localized to the flagella, indicating a clear differentiation between compartmentalization of PKA and calcineurin pathways. Moreover, no crosstalk is observed between these signaling events, even though both pathways are required for progressive motility acquisition as discussed.
Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: O'brien Echeverría, Emma Dinorah. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Maidagan, Paula María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Morales, Enrique Salvador. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Visconti, Pablo. University Of Massachussets; Estados Unidos
Fil: Arranz, Cristina Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Animals with external fertilization, as amphibians, store their sperm in a quiescent state in the testis. When spermatozoa are released into natural fertilization media, the hypotonic shock triggers activation of sperm motility. Rhinella (Bufo) arenarum sperm are immotile in artificial seminal plasma (ASP, resembling testicular plasma tonicity) but acquire in situ flagellar beating upon dilution. However, if components from the egg shelly coat are added to this medium, motility shifts to a progressive pattern. Recently, we have shown that the signal transduction pathway required for in situ motility activation involves a rise in intracellular cAMP through a transmembrane adenylyl cyclase and activation of PKA, mostly in the midpiece and in the sperm head. In this report, we demonstrate that activation of calcineurin (aka PP2B and PPP3) is required for the shift from in situ to progressive sperm motility. The effect of calcineurin is manifested by dephosphorylation of PKC substrates, and can be promoted by intracellular calcium rise by Ca2+ ionophore. Both phosphorylated PKC substrates and calcineurin localized to the flagella, indicating a clear differentiation between compartmentalization of PKA and calcineurin pathways. Moreover, no crosstalk is observed between these signaling events, even though both pathways are required for progressive motility acquisition as discussed.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8619
Krapf, Dario; O'brien Echeverría, Emma Dinorah; Maidagan, Paula María; Morales, Enrique Salvador; Visconti, Pablo; et al.; Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates; Wiley; Journal Of Cellular Physiology; 229; 10; 10-2014; 1378-1386
0021-9541
url http://hdl.handle.net/11336/8619
identifier_str_mv Krapf, Dario; O'brien Echeverría, Emma Dinorah; Maidagan, Paula María; Morales, Enrique Salvador; Visconti, Pablo; et al.; Calcineurin Regulates Progressive Motility Activation of Rhinella (Bufo) arenarum Sperm Through Dephosphorylation of PKC Substrates; Wiley; Journal Of Cellular Physiology; 229; 10; 10-2014; 1378-1386
0021-9541
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jcp.24571
info:eu-repo/semantics/altIdentifier/doi/10.1002/jcp.24571
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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