Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa
- Autores
- Calamera, Juan C.; Buffone, Mariano Gabriel; Doncel, Gustavo F.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; Calamera, Maria M.; Storey, Bayard T.; Alvarez, Juan G.
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Objective To investigate the effects of thawing temperature on sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Eighty consenting normozoospermic patients consulting for infertility. Intervention(s) Spermatozoa from donor semen samples were thawed at different temperatures. Main Outcome Measure(s) Sperm motility, viability, adenosine-5'-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. Result(s) Thawing at 40°C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20°C and 37°C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content, and DNA integrity after thawing at 40°C compared with thawing at temperatures between 20°C and 37°C. Conclusion(s) Sperm thawing at 40°C could be safely used to improve motility recovery after sperm cryopreservation.
Fil: Calamera, Juan C.. Laboratorio de Estudios de la Reproducción LER; Argentina
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina. University of Pennsylvania; Estados Unidos
Fil: Doncel, Gustavo F.. Eastern Virginia Medical School. Jones Institute For Reproductive Medicine; Estados Unidos
Fil: Brugo Olmedo, Santiago. Centro Médico Seremas; Argentina
Fil: de Vincentiis, Sabrina. Centro Médico Seremas; Argentina
Fil: Calamera, Maria M.. Laboratorio de Estudios de la Reproducción LER; Argentina
Fil: Storey, Bayard T.. University of Pennsylvania; Estados Unidos
Fil: Alvarez, Juan G.. Instituto Marques; España. Fundación Leonardo Marques; España - Materia
-
Sperm
Criopreservation
Temperature
Dna Damage - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/14695
Ver los metadatos del registro completo
id |
CONICETDig_66ff0212a3e05b59dedb01310bff22c2 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/14695 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoaCalamera, Juan C.Buffone, Mariano GabrielDoncel, Gustavo F.Brugo Olmedo, Santiagode Vincentiis, SabrinaCalamera, Maria M.Storey, Bayard T.Alvarez, Juan G.SpermCriopreservationTemperatureDna Damagehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective To investigate the effects of thawing temperature on sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Eighty consenting normozoospermic patients consulting for infertility. Intervention(s) Spermatozoa from donor semen samples were thawed at different temperatures. Main Outcome Measure(s) Sperm motility, viability, adenosine-5'-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. Result(s) Thawing at 40°C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20°C and 37°C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content, and DNA integrity after thawing at 40°C compared with thawing at temperatures between 20°C and 37°C. Conclusion(s) Sperm thawing at 40°C could be safely used to improve motility recovery after sperm cryopreservation.Fil: Calamera, Juan C.. Laboratorio de Estudios de la Reproducción LER; ArgentinaFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina. University of Pennsylvania; Estados UnidosFil: Doncel, Gustavo F.. Eastern Virginia Medical School. Jones Institute For Reproductive Medicine; Estados UnidosFil: Brugo Olmedo, Santiago. Centro Médico Seremas; ArgentinaFil: de Vincentiis, Sabrina. Centro Médico Seremas; ArgentinaFil: Calamera, Maria M.. Laboratorio de Estudios de la Reproducción LER; ArgentinaFil: Storey, Bayard T.. University of Pennsylvania; Estados UnidosFil: Alvarez, Juan G.. Instituto Marques; España. Fundación Leonardo Marques; EspañaElsevier2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14695Calamera, Juan C.; Buffone, Mariano Gabriel; Doncel, Gustavo F.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; et al.; Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa; Elsevier; Fertility And Sterility; 93; 3; 2-2010; 789-7940015-02821556-5653enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2008.10.021info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0015028208042830info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:28Zoai:ri.conicet.gov.ar:11336/14695instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:28.938CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa |
title |
Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa |
spellingShingle |
Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa Calamera, Juan C. Sperm Criopreservation Temperature Dna Damage |
title_short |
Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa |
title_full |
Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa |
title_fullStr |
Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa |
title_full_unstemmed |
Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa |
title_sort |
Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa |
dc.creator.none.fl_str_mv |
Calamera, Juan C. Buffone, Mariano Gabriel Doncel, Gustavo F. Brugo Olmedo, Santiago de Vincentiis, Sabrina Calamera, Maria M. Storey, Bayard T. Alvarez, Juan G. |
author |
Calamera, Juan C. |
author_facet |
Calamera, Juan C. Buffone, Mariano Gabriel Doncel, Gustavo F. Brugo Olmedo, Santiago de Vincentiis, Sabrina Calamera, Maria M. Storey, Bayard T. Alvarez, Juan G. |
author_role |
author |
author2 |
Buffone, Mariano Gabriel Doncel, Gustavo F. Brugo Olmedo, Santiago de Vincentiis, Sabrina Calamera, Maria M. Storey, Bayard T. Alvarez, Juan G. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Sperm Criopreservation Temperature Dna Damage |
topic |
Sperm Criopreservation Temperature Dna Damage |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Objective To investigate the effects of thawing temperature on sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Eighty consenting normozoospermic patients consulting for infertility. Intervention(s) Spermatozoa from donor semen samples were thawed at different temperatures. Main Outcome Measure(s) Sperm motility, viability, adenosine-5'-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. Result(s) Thawing at 40°C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20°C and 37°C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content, and DNA integrity after thawing at 40°C compared with thawing at temperatures between 20°C and 37°C. Conclusion(s) Sperm thawing at 40°C could be safely used to improve motility recovery after sperm cryopreservation. Fil: Calamera, Juan C.. Laboratorio de Estudios de la Reproducción LER; Argentina Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina. University of Pennsylvania; Estados Unidos Fil: Doncel, Gustavo F.. Eastern Virginia Medical School. Jones Institute For Reproductive Medicine; Estados Unidos Fil: Brugo Olmedo, Santiago. Centro Médico Seremas; Argentina Fil: de Vincentiis, Sabrina. Centro Médico Seremas; Argentina Fil: Calamera, Maria M.. Laboratorio de Estudios de la Reproducción LER; Argentina Fil: Storey, Bayard T.. University of Pennsylvania; Estados Unidos Fil: Alvarez, Juan G.. Instituto Marques; España. Fundación Leonardo Marques; España |
description |
Objective To investigate the effects of thawing temperature on sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Eighty consenting normozoospermic patients consulting for infertility. Intervention(s) Spermatozoa from donor semen samples were thawed at different temperatures. Main Outcome Measure(s) Sperm motility, viability, adenosine-5'-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. Result(s) Thawing at 40°C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20°C and 37°C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content, and DNA integrity after thawing at 40°C compared with thawing at temperatures between 20°C and 37°C. Conclusion(s) Sperm thawing at 40°C could be safely used to improve motility recovery after sperm cryopreservation. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/14695 Calamera, Juan C.; Buffone, Mariano Gabriel; Doncel, Gustavo F.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; et al.; Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa; Elsevier; Fertility And Sterility; 93; 3; 2-2010; 789-794 0015-0282 1556-5653 |
url |
http://hdl.handle.net/11336/14695 |
identifier_str_mv |
Calamera, Juan C.; Buffone, Mariano Gabriel; Doncel, Gustavo F.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; et al.; Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa; Elsevier; Fertility And Sterility; 93; 3; 2-2010; 789-794 0015-0282 1556-5653 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2008.10.021 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0015028208042830 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842270159587246080 |
score |
13.13397 |