Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa

Autores
Calamera, Juan C.; Buffone, Mariano Gabriel; Doncel, Gustavo F.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; Calamera, Maria M.; Storey, Bayard T.; Alvarez, Juan G.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective To investigate the effects of thawing temperature on sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Eighty consenting normozoospermic patients consulting for infertility. Intervention(s) Spermatozoa from donor semen samples were thawed at different temperatures. Main Outcome Measure(s) Sperm motility, viability, adenosine-5'-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. Result(s) Thawing at 40°C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20°C and 37°C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content, and DNA integrity after thawing at 40°C compared with thawing at temperatures between 20°C and 37°C. Conclusion(s) Sperm thawing at 40°C could be safely used to improve motility recovery after sperm cryopreservation.
Fil: Calamera, Juan C.. Laboratorio de Estudios de la Reproducción LER; Argentina
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina. University of Pennsylvania; Estados Unidos
Fil: Doncel, Gustavo F.. Eastern Virginia Medical School. Jones Institute For Reproductive Medicine; Estados Unidos
Fil: Brugo Olmedo, Santiago. Centro Médico Seremas; Argentina
Fil: de Vincentiis, Sabrina. Centro Médico Seremas; Argentina
Fil: Calamera, Maria M.. Laboratorio de Estudios de la Reproducción LER; Argentina
Fil: Storey, Bayard T.. University of Pennsylvania; Estados Unidos
Fil: Alvarez, Juan G.. Instituto Marques; España. Fundación Leonardo Marques; España
Materia
Sperm
Criopreservation
Temperature
Dna Damage
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14695
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14695
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoaCalamera, Juan C.Buffone, Mariano GabrielDoncel, Gustavo F.Brugo Olmedo, Santiagode Vincentiis, SabrinaCalamera, Maria M.Storey, Bayard T.Alvarez, Juan G.SpermCriopreservationTemperatureDna Damagehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective To investigate the effects of thawing temperature on sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Eighty consenting normozoospermic patients consulting for infertility. Intervention(s) Spermatozoa from donor semen samples were thawed at different temperatures. Main Outcome Measure(s) Sperm motility, viability, adenosine-5'-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. Result(s) Thawing at 40°C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20°C and 37°C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content, and DNA integrity after thawing at 40°C compared with thawing at temperatures between 20°C and 37°C. Conclusion(s) Sperm thawing at 40°C could be safely used to improve motility recovery after sperm cryopreservation.Fil: Calamera, Juan C.. Laboratorio de Estudios de la Reproducción LER; ArgentinaFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina. University of Pennsylvania; Estados UnidosFil: Doncel, Gustavo F.. Eastern Virginia Medical School. Jones Institute For Reproductive Medicine; Estados UnidosFil: Brugo Olmedo, Santiago. Centro Médico Seremas; ArgentinaFil: de Vincentiis, Sabrina. Centro Médico Seremas; ArgentinaFil: Calamera, Maria M.. Laboratorio de Estudios de la Reproducción LER; ArgentinaFil: Storey, Bayard T.. University of Pennsylvania; Estados UnidosFil: Alvarez, Juan G.. Instituto Marques; España. Fundación Leonardo Marques; EspañaElsevier2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14695Calamera, Juan C.; Buffone, Mariano Gabriel; Doncel, Gustavo F.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; et al.; Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa; Elsevier; Fertility And Sterility; 93; 3; 2-2010; 789-7940015-02821556-5653enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2008.10.021info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0015028208042830info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:54Zoai:ri.conicet.gov.ar:11336/14695instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:55.067CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa
title Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa
spellingShingle Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa
Calamera, Juan C.
Sperm
Criopreservation
Temperature
Dna Damage
title_short Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa
title_full Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa
title_fullStr Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa
title_full_unstemmed Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa
title_sort Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa
dc.creator.none.fl_str_mv Calamera, Juan C.
Buffone, Mariano Gabriel
Doncel, Gustavo F.
Brugo Olmedo, Santiago
de Vincentiis, Sabrina
Calamera, Maria M.
Storey, Bayard T.
Alvarez, Juan G.
author Calamera, Juan C.
author_facet Calamera, Juan C.
Buffone, Mariano Gabriel
Doncel, Gustavo F.
Brugo Olmedo, Santiago
de Vincentiis, Sabrina
Calamera, Maria M.
Storey, Bayard T.
Alvarez, Juan G.
author_role author
author2 Buffone, Mariano Gabriel
Doncel, Gustavo F.
Brugo Olmedo, Santiago
de Vincentiis, Sabrina
Calamera, Maria M.
Storey, Bayard T.
Alvarez, Juan G.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Sperm
Criopreservation
Temperature
Dna Damage
topic Sperm
Criopreservation
Temperature
Dna Damage
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective To investigate the effects of thawing temperature on sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Eighty consenting normozoospermic patients consulting for infertility. Intervention(s) Spermatozoa from donor semen samples were thawed at different temperatures. Main Outcome Measure(s) Sperm motility, viability, adenosine-5'-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. Result(s) Thawing at 40°C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20°C and 37°C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content, and DNA integrity after thawing at 40°C compared with thawing at temperatures between 20°C and 37°C. Conclusion(s) Sperm thawing at 40°C could be safely used to improve motility recovery after sperm cryopreservation.
Fil: Calamera, Juan C.. Laboratorio de Estudios de la Reproducción LER; Argentina
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina. University of Pennsylvania; Estados Unidos
Fil: Doncel, Gustavo F.. Eastern Virginia Medical School. Jones Institute For Reproductive Medicine; Estados Unidos
Fil: Brugo Olmedo, Santiago. Centro Médico Seremas; Argentina
Fil: de Vincentiis, Sabrina. Centro Médico Seremas; Argentina
Fil: Calamera, Maria M.. Laboratorio de Estudios de la Reproducción LER; Argentina
Fil: Storey, Bayard T.. University of Pennsylvania; Estados Unidos
Fil: Alvarez, Juan G.. Instituto Marques; España. Fundación Leonardo Marques; España
description Objective To investigate the effects of thawing temperature on sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Eighty consenting normozoospermic patients consulting for infertility. Intervention(s) Spermatozoa from donor semen samples were thawed at different temperatures. Main Outcome Measure(s) Sperm motility, viability, adenosine-5'-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. Result(s) Thawing at 40°C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20°C and 37°C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content, and DNA integrity after thawing at 40°C compared with thawing at temperatures between 20°C and 37°C. Conclusion(s) Sperm thawing at 40°C could be safely used to improve motility recovery after sperm cryopreservation.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14695
Calamera, Juan C.; Buffone, Mariano Gabriel; Doncel, Gustavo F.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; et al.; Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa; Elsevier; Fertility And Sterility; 93; 3; 2-2010; 789-794
0015-0282
1556-5653
url http://hdl.handle.net/11336/14695
identifier_str_mv Calamera, Juan C.; Buffone, Mariano Gabriel; Doncel, Gustavo F.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; et al.; Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa; Elsevier; Fertility And Sterility; 93; 3; 2-2010; 789-794
0015-0282
1556-5653
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2008.10.021
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0015028208042830
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614499783409664
score 13.070432

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