Biocontrol of Penicillium digitatum by native Bacillus and Pseudomonas strains isolated from orange peel

Autores
Ferreira, Flavia Vanina; Bello, Fernando; Rivadeneira, Maria Fernanda; Vazquez, Daniel Eduardo; Musumeci, Matías Alejandro
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Penicillium digitatum is a filamentous fungus that infects citrus fruits, causing decays that result in significant production losses. The application of synthetic fungicides is the main approach to control P. digitatum. However, intensive usage of fungicides has led to the proliferation of resistant P. digitatum strains. Besides, this practice poses a risk for the human and environmental health and is incompatible with organic markets. Alternative approaches that may overcome these limitations are valuable innovations for citrus production. The objective of this work was to evaluate the potential of bacterial strains inhabiting naturally in oranges to control the growth of sensitive and resistant to pyrimethanil P. digitatum strains. Around 50 bacteria were isolated from oranges peel but only three showed optimal antagonistic activity against sensitive P. digitatum after qualitative screening assays. These bacteria were identified by molecular and biochemical analyses and corresponded to Bacillus mojavensis SC-45, Bacillus velezensis SC-31 and Pseudomonas psychrotolerans SC-29. The antifungal activity of bacterial extracellular cultures against P. digitatum strains was quantified in vitro by the poison agar method and by measuring the mycelial growth at 600 nm in a microplate assay. B. velezensis SC-31 showed the highest growth inhibition of both P. digitatum strains. Lipopeptides with antifungal activity were identified by HPLC-ESI-MS in bacterial extracellular cultures. Surfactin and iturin were the most abundant lipopeptides produced by Bacillus spp., but higher yields were observed in B. velezensis SC-31 supernatant; whereas viscosin was mainly produced by P. psychrotolerans SC-29. B. velezensis SC-31 also showed the highest proteolytic activity and the highest capability to form biofilms. B. velezensis SC-31 and B. mojavensis SC-45 produced the lowest incidence and severity of disease in vivo after infection with both P. digitatum strains in preventive treatments. Besides, bacteria were compatible with concentrations of fungicides corresponding to maximum residue levels (MRL) on citrus fruits and higher concentrations used in drenches. B. velezensis SC-31 and B. mojavensis SC-45 are proficient antagonists to control green mould in oranges, which can be used together with fungicides to improve the inhibition of P. digitatum and avoid the emergence of resistant strains.
EEA Concordia
Fil: Ferreira, Flavia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Rivadeneira, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Musumeci, Matías Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Musumeci, Matías Alejandro. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fuente
Biological Control 186 : 105340. (November 2023)
Materia
Penicillium digitatum
Bacillus
Pseudomonas
Control Biológico
Naranja Dulce
Piel (vegetal)
Biological Control
Sweet Oranges
Peel
Biocontrol
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15140
Acceder
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oai_identifier_str oai:localhost:20.500.12123/15140
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Biocontrol of Penicillium digitatum by native Bacillus and Pseudomonas strains isolated from orange peelFerreira, Flavia VaninaBello, FernandoRivadeneira, Maria FernandaVazquez, Daniel EduardoMusumeci, Matías AlejandroPenicillium digitatumBacillusPseudomonasControl BiológicoNaranja DulcePiel (vegetal)Biological ControlSweet OrangesPeelBiocontrolPenicillium digitatum is a filamentous fungus that infects citrus fruits, causing decays that result in significant production losses. The application of synthetic fungicides is the main approach to control P. digitatum. However, intensive usage of fungicides has led to the proliferation of resistant P. digitatum strains. Besides, this practice poses a risk for the human and environmental health and is incompatible with organic markets. Alternative approaches that may overcome these limitations are valuable innovations for citrus production. The objective of this work was to evaluate the potential of bacterial strains inhabiting naturally in oranges to control the growth of sensitive and resistant to pyrimethanil P. digitatum strains. Around 50 bacteria were isolated from oranges peel but only three showed optimal antagonistic activity against sensitive P. digitatum after qualitative screening assays. These bacteria were identified by molecular and biochemical analyses and corresponded to Bacillus mojavensis SC-45, Bacillus velezensis SC-31 and Pseudomonas psychrotolerans SC-29. The antifungal activity of bacterial extracellular cultures against P. digitatum strains was quantified in vitro by the poison agar method and by measuring the mycelial growth at 600 nm in a microplate assay. B. velezensis SC-31 showed the highest growth inhibition of both P. digitatum strains. Lipopeptides with antifungal activity were identified by HPLC-ESI-MS in bacterial extracellular cultures. Surfactin and iturin were the most abundant lipopeptides produced by Bacillus spp., but higher yields were observed in B. velezensis SC-31 supernatant; whereas viscosin was mainly produced by P. psychrotolerans SC-29. B. velezensis SC-31 also showed the highest proteolytic activity and the highest capability to form biofilms. B. velezensis SC-31 and B. mojavensis SC-45 produced the lowest incidence and severity of disease in vivo after infection with both P. digitatum strains in preventive treatments. Besides, bacteria were compatible with concentrations of fungicides corresponding to maximum residue levels (MRL) on citrus fruits and higher concentrations used in drenches. B. velezensis SC-31 and B. mojavensis SC-45 are proficient antagonists to control green mould in oranges, which can be used together with fungicides to improve the inhibition of P. digitatum and avoid the emergence of resistant strains.EEA ConcordiaFil: Ferreira, Flavia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; ArgentinaFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Rivadeneira, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Musumeci, Matías Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; ArgentinaFil: Musumeci, Matías Alejandro. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaElsevier2023-09-07T15:42:48Z2023-09-07T15:42:48Z2023-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15140https://www.sciencedirect.com/science/article/pii/S10499644230019371049-96441090-2112https://doi.org/10.1016/j.biocontrol.2023.105340Biological Control 186 : 105340. (November 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:24:42Zoai:localhost:20.500.12123/15140instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:24:42.549INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Biocontrol of Penicillium digitatum by native Bacillus and Pseudomonas strains isolated from orange peel
title Biocontrol of Penicillium digitatum by native Bacillus and Pseudomonas strains isolated from orange peel
spellingShingle Biocontrol of Penicillium digitatum by native Bacillus and Pseudomonas strains isolated from orange peel
Ferreira, Flavia Vanina
Penicillium digitatum
Bacillus
Pseudomonas
Control Biológico
Naranja Dulce
Piel (vegetal)
Biological Control
Sweet Oranges
Peel
Biocontrol
title_short Biocontrol of Penicillium digitatum by native Bacillus and Pseudomonas strains isolated from orange peel
title_full Biocontrol of Penicillium digitatum by native Bacillus and Pseudomonas strains isolated from orange peel
title_fullStr Biocontrol of Penicillium digitatum by native Bacillus and Pseudomonas strains isolated from orange peel
title_full_unstemmed Biocontrol of Penicillium digitatum by native Bacillus and Pseudomonas strains isolated from orange peel
title_sort Biocontrol of Penicillium digitatum by native Bacillus and Pseudomonas strains isolated from orange peel
dc.creator.none.fl_str_mv Ferreira, Flavia Vanina
Bello, Fernando
Rivadeneira, Maria Fernanda
Vazquez, Daniel Eduardo
Musumeci, Matías Alejandro
author Ferreira, Flavia Vanina
author_facet Ferreira, Flavia Vanina
Bello, Fernando
Rivadeneira, Maria Fernanda
Vazquez, Daniel Eduardo
Musumeci, Matías Alejandro
author_role author
author2 Bello, Fernando
Rivadeneira, Maria Fernanda
Vazquez, Daniel Eduardo
Musumeci, Matías Alejandro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Penicillium digitatum
Bacillus
Pseudomonas
Control Biológico
Naranja Dulce
Piel (vegetal)
Biological Control
Sweet Oranges
Peel
Biocontrol
topic Penicillium digitatum
Bacillus
Pseudomonas
Control Biológico
Naranja Dulce
Piel (vegetal)
Biological Control
Sweet Oranges
Peel
Biocontrol
dc.description.none.fl_txt_mv Penicillium digitatum is a filamentous fungus that infects citrus fruits, causing decays that result in significant production losses. The application of synthetic fungicides is the main approach to control P. digitatum. However, intensive usage of fungicides has led to the proliferation of resistant P. digitatum strains. Besides, this practice poses a risk for the human and environmental health and is incompatible with organic markets. Alternative approaches that may overcome these limitations are valuable innovations for citrus production. The objective of this work was to evaluate the potential of bacterial strains inhabiting naturally in oranges to control the growth of sensitive and resistant to pyrimethanil P. digitatum strains. Around 50 bacteria were isolated from oranges peel but only three showed optimal antagonistic activity against sensitive P. digitatum after qualitative screening assays. These bacteria were identified by molecular and biochemical analyses and corresponded to Bacillus mojavensis SC-45, Bacillus velezensis SC-31 and Pseudomonas psychrotolerans SC-29. The antifungal activity of bacterial extracellular cultures against P. digitatum strains was quantified in vitro by the poison agar method and by measuring the mycelial growth at 600 nm in a microplate assay. B. velezensis SC-31 showed the highest growth inhibition of both P. digitatum strains. Lipopeptides with antifungal activity were identified by HPLC-ESI-MS in bacterial extracellular cultures. Surfactin and iturin were the most abundant lipopeptides produced by Bacillus spp., but higher yields were observed in B. velezensis SC-31 supernatant; whereas viscosin was mainly produced by P. psychrotolerans SC-29. B. velezensis SC-31 also showed the highest proteolytic activity and the highest capability to form biofilms. B. velezensis SC-31 and B. mojavensis SC-45 produced the lowest incidence and severity of disease in vivo after infection with both P. digitatum strains in preventive treatments. Besides, bacteria were compatible with concentrations of fungicides corresponding to maximum residue levels (MRL) on citrus fruits and higher concentrations used in drenches. B. velezensis SC-31 and B. mojavensis SC-45 are proficient antagonists to control green mould in oranges, which can be used together with fungicides to improve the inhibition of P. digitatum and avoid the emergence of resistant strains.
EEA Concordia
Fil: Ferreira, Flavia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Rivadeneira, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Musumeci, Matías Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Musumeci, Matías Alejandro. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
description Penicillium digitatum is a filamentous fungus that infects citrus fruits, causing decays that result in significant production losses. The application of synthetic fungicides is the main approach to control P. digitatum. However, intensive usage of fungicides has led to the proliferation of resistant P. digitatum strains. Besides, this practice poses a risk for the human and environmental health and is incompatible with organic markets. Alternative approaches that may overcome these limitations are valuable innovations for citrus production. The objective of this work was to evaluate the potential of bacterial strains inhabiting naturally in oranges to control the growth of sensitive and resistant to pyrimethanil P. digitatum strains. Around 50 bacteria were isolated from oranges peel but only three showed optimal antagonistic activity against sensitive P. digitatum after qualitative screening assays. These bacteria were identified by molecular and biochemical analyses and corresponded to Bacillus mojavensis SC-45, Bacillus velezensis SC-31 and Pseudomonas psychrotolerans SC-29. The antifungal activity of bacterial extracellular cultures against P. digitatum strains was quantified in vitro by the poison agar method and by measuring the mycelial growth at 600 nm in a microplate assay. B. velezensis SC-31 showed the highest growth inhibition of both P. digitatum strains. Lipopeptides with antifungal activity were identified by HPLC-ESI-MS in bacterial extracellular cultures. Surfactin and iturin were the most abundant lipopeptides produced by Bacillus spp., but higher yields were observed in B. velezensis SC-31 supernatant; whereas viscosin was mainly produced by P. psychrotolerans SC-29. B. velezensis SC-31 also showed the highest proteolytic activity and the highest capability to form biofilms. B. velezensis SC-31 and B. mojavensis SC-45 produced the lowest incidence and severity of disease in vivo after infection with both P. digitatum strains in preventive treatments. Besides, bacteria were compatible with concentrations of fungicides corresponding to maximum residue levels (MRL) on citrus fruits and higher concentrations used in drenches. B. velezensis SC-31 and B. mojavensis SC-45 are proficient antagonists to control green mould in oranges, which can be used together with fungicides to improve the inhibition of P. digitatum and avoid the emergence of resistant strains.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-07T15:42:48Z
2023-09-07T15:42:48Z
2023-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15140
https://www.sciencedirect.com/science/article/pii/S1049964423001937
1049-9644
1090-2112
https://doi.org/10.1016/j.biocontrol.2023.105340
url http://hdl.handle.net/20.500.12123/15140
https://www.sciencedirect.com/science/article/pii/S1049964423001937
https://doi.org/10.1016/j.biocontrol.2023.105340
identifier_str_mv 1049-9644
1090-2112
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Biological Control 186 : 105340. (November 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.993085

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