LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton

Autores
Sanchez, Angel Matias; Flamini, Marina Ines; Russo, Eleonora; Casarosa, Elena; Pacini, Simone; Petrini, Mario; Genazzani, Andrea Riccardo; Simoncini, Tommaso
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Reproductive hormones influence breast cancer development and progression. While the actions of sex steroids in this setting are established, tentative evidence suggests that follicle-stimulating hormone (FSH) and luteinizing hormone (LH) may also play a role, yet this remains elusive. We here identify that T-47D breast cancer cells express functional receptors for FSH and LH, and that these hormones regulate breast cancer cell motility and invasion through the control of the actin cytoskeleton and the formation of cortical actin aggregates and focal adhesion complexes. Such actions are mediated by the cytoskeletal controllers Moesin and focal adhesion kinase (FAK). Moesin is recruited rapidly by FSH and LH through a signaling cascade requiring the G protein Gα13 and the Rho-associated kinase, ROCK-2. FSH and LH activate FAK via a Gαi/β and c-Src-dependent signaling cascade. Both cascades involve signaling to phosphatidylinositol-3 kinase and Akt. FSH and LH receptors and the related signaling intermediates are necessary for the actions of gonadotrophins on breast cancer cell cytoskeletal rearrangement, migration and invasion. These findings provide original information on the actions of gonadotrophins on breast cancer cells and may have clinical implications for the use of drugs that modulate gonadotrophins in breast cancer patients.
Fil: Sanchez, Angel Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Flamini, Marina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Russo, Eleonora. Università degli Studi di Pisa; Italia
Fil: Casarosa, Elena. Università degli Studi di Pisa; Italia
Fil: Pacini, Simone. Università degli Studi di Pisa; Italia
Fil: Petrini, Mario. Università degli Studi di Pisa; Italia
Fil: Genazzani, Andrea Riccardo. Università degli Studi di Pisa; Italia
Fil: Simoncini, Tommaso. Università degli Studi di Pisa; Italia
Materia
BREAST CANCER
FSH
INVASION
LH
MIGRATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182640
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/182640
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeletonSanchez, Angel MatiasFlamini, Marina InesRusso, EleonoraCasarosa, ElenaPacini, SimonePetrini, MarioGenazzani, Andrea RiccardoSimoncini, TommasoBREAST CANCERFSHINVASIONLHMIGRATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Reproductive hormones influence breast cancer development and progression. While the actions of sex steroids in this setting are established, tentative evidence suggests that follicle-stimulating hormone (FSH) and luteinizing hormone (LH) may also play a role, yet this remains elusive. We here identify that T-47D breast cancer cells express functional receptors for FSH and LH, and that these hormones regulate breast cancer cell motility and invasion through the control of the actin cytoskeleton and the formation of cortical actin aggregates and focal adhesion complexes. Such actions are mediated by the cytoskeletal controllers Moesin and focal adhesion kinase (FAK). Moesin is recruited rapidly by FSH and LH through a signaling cascade requiring the G protein Gα13 and the Rho-associated kinase, ROCK-2. FSH and LH activate FAK via a Gαi/β and c-Src-dependent signaling cascade. Both cascades involve signaling to phosphatidylinositol-3 kinase and Akt. FSH and LH receptors and the related signaling intermediates are necessary for the actions of gonadotrophins on breast cancer cell cytoskeletal rearrangement, migration and invasion. These findings provide original information on the actions of gonadotrophins on breast cancer cells and may have clinical implications for the use of drugs that modulate gonadotrophins in breast cancer patients.Fil: Sanchez, Angel Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Flamini, Marina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Russo, Eleonora. Università degli Studi di Pisa; ItaliaFil: Casarosa, Elena. Università degli Studi di Pisa; ItaliaFil: Pacini, Simone. Università degli Studi di Pisa; ItaliaFil: Petrini, Mario. Università degli Studi di Pisa; ItaliaFil: Genazzani, Andrea Riccardo. Università degli Studi di Pisa; ItaliaFil: Simoncini, Tommaso. Università degli Studi di Pisa; ItaliaElsevier Ireland2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182640Sanchez, Angel Matias; Flamini, Marina Ines; Russo, Eleonora; Casarosa, Elena; Pacini, Simone; et al.; LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton; Elsevier Ireland; Molecular and Cellular Endocrinology; 437; 8-2016; 22-340303-7207CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2016.08.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:14Zoai:ri.conicet.gov.ar:11336/182640instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:14.356CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton
title LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton
spellingShingle LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton
Sanchez, Angel Matias
BREAST CANCER
FSH
INVASION
LH
MIGRATION
title_short LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton
title_full LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton
title_fullStr LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton
title_full_unstemmed LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton
title_sort LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton
dc.creator.none.fl_str_mv Sanchez, Angel Matias
Flamini, Marina Ines
Russo, Eleonora
Casarosa, Elena
Pacini, Simone
Petrini, Mario
Genazzani, Andrea Riccardo
Simoncini, Tommaso
author Sanchez, Angel Matias
author_facet Sanchez, Angel Matias
Flamini, Marina Ines
Russo, Eleonora
Casarosa, Elena
Pacini, Simone
Petrini, Mario
Genazzani, Andrea Riccardo
Simoncini, Tommaso
author_role author
author2 Flamini, Marina Ines
Russo, Eleonora
Casarosa, Elena
Pacini, Simone
Petrini, Mario
Genazzani, Andrea Riccardo
Simoncini, Tommaso
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BREAST CANCER
FSH
INVASION
LH
MIGRATION
topic BREAST CANCER
FSH
INVASION
LH
MIGRATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Reproductive hormones influence breast cancer development and progression. While the actions of sex steroids in this setting are established, tentative evidence suggests that follicle-stimulating hormone (FSH) and luteinizing hormone (LH) may also play a role, yet this remains elusive. We here identify that T-47D breast cancer cells express functional receptors for FSH and LH, and that these hormones regulate breast cancer cell motility and invasion through the control of the actin cytoskeleton and the formation of cortical actin aggregates and focal adhesion complexes. Such actions are mediated by the cytoskeletal controllers Moesin and focal adhesion kinase (FAK). Moesin is recruited rapidly by FSH and LH through a signaling cascade requiring the G protein Gα13 and the Rho-associated kinase, ROCK-2. FSH and LH activate FAK via a Gαi/β and c-Src-dependent signaling cascade. Both cascades involve signaling to phosphatidylinositol-3 kinase and Akt. FSH and LH receptors and the related signaling intermediates are necessary for the actions of gonadotrophins on breast cancer cell cytoskeletal rearrangement, migration and invasion. These findings provide original information on the actions of gonadotrophins on breast cancer cells and may have clinical implications for the use of drugs that modulate gonadotrophins in breast cancer patients.
Fil: Sanchez, Angel Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Flamini, Marina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Russo, Eleonora. Università degli Studi di Pisa; Italia
Fil: Casarosa, Elena. Università degli Studi di Pisa; Italia
Fil: Pacini, Simone. Università degli Studi di Pisa; Italia
Fil: Petrini, Mario. Università degli Studi di Pisa; Italia
Fil: Genazzani, Andrea Riccardo. Università degli Studi di Pisa; Italia
Fil: Simoncini, Tommaso. Università degli Studi di Pisa; Italia
description Reproductive hormones influence breast cancer development and progression. While the actions of sex steroids in this setting are established, tentative evidence suggests that follicle-stimulating hormone (FSH) and luteinizing hormone (LH) may also play a role, yet this remains elusive. We here identify that T-47D breast cancer cells express functional receptors for FSH and LH, and that these hormones regulate breast cancer cell motility and invasion through the control of the actin cytoskeleton and the formation of cortical actin aggregates and focal adhesion complexes. Such actions are mediated by the cytoskeletal controllers Moesin and focal adhesion kinase (FAK). Moesin is recruited rapidly by FSH and LH through a signaling cascade requiring the G protein Gα13 and the Rho-associated kinase, ROCK-2. FSH and LH activate FAK via a Gαi/β and c-Src-dependent signaling cascade. Both cascades involve signaling to phosphatidylinositol-3 kinase and Akt. FSH and LH receptors and the related signaling intermediates are necessary for the actions of gonadotrophins on breast cancer cell cytoskeletal rearrangement, migration and invasion. These findings provide original information on the actions of gonadotrophins on breast cancer cells and may have clinical implications for the use of drugs that modulate gonadotrophins in breast cancer patients.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182640
Sanchez, Angel Matias; Flamini, Marina Ines; Russo, Eleonora; Casarosa, Elena; Pacini, Simone; et al.; LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton; Elsevier Ireland; Molecular and Cellular Endocrinology; 437; 8-2016; 22-34
0303-7207
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182640
identifier_str_mv Sanchez, Angel Matias; Flamini, Marina Ines; Russo, Eleonora; Casarosa, Elena; Pacini, Simone; et al.; LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton; Elsevier Ireland; Molecular and Cellular Endocrinology; 437; 8-2016; 22-34
0303-7207
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2016.08.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269789896048640
score 13.13397

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