Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats

Autores
Sanchez, Angel Matias; Flamini, Marina Ines; Zullino, Sara; Russo, Eleonora; Giannini, Andrea; Mannella, Paolo; Naccarato, Antonio Giuseppe; Genazzani, Andrea Ricardo; Simoncinii, Tommaso
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gonadotrophins are mainly known to influence the body through the formation of gonadal steroids. However, receptors for luteinizing hormone (LH) and follicular-stimulating hormone (FSH) are present in a set of extra-gonadal tissues in humans and animals, but their functional relevance is uncertain. In this article, we present experimental evidence that, in T-47D breast cancer (BC) cells, FSH, and LH alter the expression of genes involved in adhesion, motility, and invasion through the activation of their receptors. Using miniarray technology we also found that LH influences the expression of a broad set of genes involved in cancer biology in T-47D cells. Interestingly, the regulatory actions of FSH and LH depend on the modality of exposure, with significant differences between pre-pubertal-like vs. post-menopausal-like amounts of gonadotrophins, but not after intermittent administration, representative of fertile life. We also studied the modulation of the circulating levels of gonadotrophins in an in vivo rat model of BC progression and observed a direct correlation with the extent of cancer growth. These results support the hypothesis that gonadotrophins may have direct effects on extra-gonadal tissues. They also highlight that gonadotrophins could potentially contribute to BC progression, particularly in post-menopausal women who typically have higher gonadotrophin levels. This research may ultimately lead to testing the use of gonadotrophin-modulating drugs in BC patients.
Fil: Sanchez, Angel Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Flamini, Marina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Zullino, Sara. Università degli Studi di Pisa; Italia
Fil: Russo, Eleonora. Università degli Studi di Pisa; Italia
Fil: Giannini, Andrea. Università degli Studi di Pisa; Italia
Fil: Mannella, Paolo. Università degli Studi di Pisa; Italia
Fil: Naccarato, Antonio Giuseppe. Università degli Studi di Pisa; Italia
Fil: Genazzani, Andrea Ricardo. Università degli Studi di Pisa; Italia
Fil: Simoncinii, Tommaso. Università degli Studi di Pisa; Italia
Materia
BREAST CANCER
CELL MOTILITY
FOLLICLE STIMULATING HORMONE
LH
MOESIN AND FOCAL ADHESION KINASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90556
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/90556
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in ratsSanchez, Angel MatiasFlamini, Marina InesZullino, SaraRusso, EleonoraGiannini, AndreaMannella, PaoloNaccarato, Antonio GiuseppeGenazzani, Andrea RicardoSimoncinii, TommasoBREAST CANCERCELL MOTILITYFOLLICLE STIMULATING HORMONELHMOESIN AND FOCAL ADHESION KINASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Gonadotrophins are mainly known to influence the body through the formation of gonadal steroids. However, receptors for luteinizing hormone (LH) and follicular-stimulating hormone (FSH) are present in a set of extra-gonadal tissues in humans and animals, but their functional relevance is uncertain. In this article, we present experimental evidence that, in T-47D breast cancer (BC) cells, FSH, and LH alter the expression of genes involved in adhesion, motility, and invasion through the activation of their receptors. Using miniarray technology we also found that LH influences the expression of a broad set of genes involved in cancer biology in T-47D cells. Interestingly, the regulatory actions of FSH and LH depend on the modality of exposure, with significant differences between pre-pubertal-like vs. post-menopausal-like amounts of gonadotrophins, but not after intermittent administration, representative of fertile life. We also studied the modulation of the circulating levels of gonadotrophins in an in vivo rat model of BC progression and observed a direct correlation with the extent of cancer growth. These results support the hypothesis that gonadotrophins may have direct effects on extra-gonadal tissues. They also highlight that gonadotrophins could potentially contribute to BC progression, particularly in post-menopausal women who typically have higher gonadotrophin levels. This research may ultimately lead to testing the use of gonadotrophin-modulating drugs in BC patients.Fil: Sanchez, Angel Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Flamini, Marina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Zullino, Sara. Università degli Studi di Pisa; ItaliaFil: Russo, Eleonora. Università degli Studi di Pisa; ItaliaFil: Giannini, Andrea. Università degli Studi di Pisa; ItaliaFil: Mannella, Paolo. Università degli Studi di Pisa; ItaliaFil: Naccarato, Antonio Giuseppe. Università degli Studi di Pisa; ItaliaFil: Genazzani, Andrea Ricardo. Università degli Studi di Pisa; ItaliaFil: Simoncinii, Tommaso. Università degli Studi di Pisa; ItaliaFrontiers Research Foundation2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90556Sanchez, Angel Matias; Flamini, Marina Ines; Zullino, Sara; Russo, Eleonora; Giannini, Andrea; et al.; Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats; Frontiers Research Foundation; Frontiers in Endocrinology; 9; MAY; 5-2018; 1-161664-2392CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fendo.2018.00239info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fendo.2018.00239/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:01:02Zoai:ri.conicet.gov.ar:11336/90556instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:01:02.493CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats
title Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats
spellingShingle Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats
Sanchez, Angel Matias
BREAST CANCER
CELL MOTILITY
FOLLICLE STIMULATING HORMONE
LH
MOESIN AND FOCAL ADHESION KINASE
title_short Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats
title_full Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats
title_fullStr Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats
title_full_unstemmed Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats
title_sort Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats
dc.creator.none.fl_str_mv Sanchez, Angel Matias
Flamini, Marina Ines
Zullino, Sara
Russo, Eleonora
Giannini, Andrea
Mannella, Paolo
Naccarato, Antonio Giuseppe
Genazzani, Andrea Ricardo
Simoncinii, Tommaso
author Sanchez, Angel Matias
author_facet Sanchez, Angel Matias
Flamini, Marina Ines
Zullino, Sara
Russo, Eleonora
Giannini, Andrea
Mannella, Paolo
Naccarato, Antonio Giuseppe
Genazzani, Andrea Ricardo
Simoncinii, Tommaso
author_role author
author2 Flamini, Marina Ines
Zullino, Sara
Russo, Eleonora
Giannini, Andrea
Mannella, Paolo
Naccarato, Antonio Giuseppe
Genazzani, Andrea Ricardo
Simoncinii, Tommaso
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BREAST CANCER
CELL MOTILITY
FOLLICLE STIMULATING HORMONE
LH
MOESIN AND FOCAL ADHESION KINASE
topic BREAST CANCER
CELL MOTILITY
FOLLICLE STIMULATING HORMONE
LH
MOESIN AND FOCAL ADHESION KINASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Gonadotrophins are mainly known to influence the body through the formation of gonadal steroids. However, receptors for luteinizing hormone (LH) and follicular-stimulating hormone (FSH) are present in a set of extra-gonadal tissues in humans and animals, but their functional relevance is uncertain. In this article, we present experimental evidence that, in T-47D breast cancer (BC) cells, FSH, and LH alter the expression of genes involved in adhesion, motility, and invasion through the activation of their receptors. Using miniarray technology we also found that LH influences the expression of a broad set of genes involved in cancer biology in T-47D cells. Interestingly, the regulatory actions of FSH and LH depend on the modality of exposure, with significant differences between pre-pubertal-like vs. post-menopausal-like amounts of gonadotrophins, but not after intermittent administration, representative of fertile life. We also studied the modulation of the circulating levels of gonadotrophins in an in vivo rat model of BC progression and observed a direct correlation with the extent of cancer growth. These results support the hypothesis that gonadotrophins may have direct effects on extra-gonadal tissues. They also highlight that gonadotrophins could potentially contribute to BC progression, particularly in post-menopausal women who typically have higher gonadotrophin levels. This research may ultimately lead to testing the use of gonadotrophin-modulating drugs in BC patients.
Fil: Sanchez, Angel Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Flamini, Marina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Zullino, Sara. Università degli Studi di Pisa; Italia
Fil: Russo, Eleonora. Università degli Studi di Pisa; Italia
Fil: Giannini, Andrea. Università degli Studi di Pisa; Italia
Fil: Mannella, Paolo. Università degli Studi di Pisa; Italia
Fil: Naccarato, Antonio Giuseppe. Università degli Studi di Pisa; Italia
Fil: Genazzani, Andrea Ricardo. Università degli Studi di Pisa; Italia
Fil: Simoncinii, Tommaso. Università degli Studi di Pisa; Italia
description Gonadotrophins are mainly known to influence the body through the formation of gonadal steroids. However, receptors for luteinizing hormone (LH) and follicular-stimulating hormone (FSH) are present in a set of extra-gonadal tissues in humans and animals, but their functional relevance is uncertain. In this article, we present experimental evidence that, in T-47D breast cancer (BC) cells, FSH, and LH alter the expression of genes involved in adhesion, motility, and invasion through the activation of their receptors. Using miniarray technology we also found that LH influences the expression of a broad set of genes involved in cancer biology in T-47D cells. Interestingly, the regulatory actions of FSH and LH depend on the modality of exposure, with significant differences between pre-pubertal-like vs. post-menopausal-like amounts of gonadotrophins, but not after intermittent administration, representative of fertile life. We also studied the modulation of the circulating levels of gonadotrophins in an in vivo rat model of BC progression and observed a direct correlation with the extent of cancer growth. These results support the hypothesis that gonadotrophins may have direct effects on extra-gonadal tissues. They also highlight that gonadotrophins could potentially contribute to BC progression, particularly in post-menopausal women who typically have higher gonadotrophin levels. This research may ultimately lead to testing the use of gonadotrophin-modulating drugs in BC patients.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90556
Sanchez, Angel Matias; Flamini, Marina Ines; Zullino, Sara; Russo, Eleonora; Giannini, Andrea; et al.; Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats; Frontiers Research Foundation; Frontiers in Endocrinology; 9; MAY; 5-2018; 1-16
1664-2392
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90556
identifier_str_mv Sanchez, Angel Matias; Flamini, Marina Ines; Zullino, Sara; Russo, Eleonora; Giannini, Andrea; et al.; Regulatory actions of LH and follicle-stimulating hormone on breast cancer cells and mammary tumors in rats; Frontiers Research Foundation; Frontiers in Endocrinology; 9; MAY; 5-2018; 1-16
1664-2392
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fendo.2018.00239
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fendo.2018.00239/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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