Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii

Autores
Sáenz, María Elena; Bišová, Kateřina; Touloupakis, Eleftherios; Faraloni, Cecilia; Di Marzio, Walter Dario; Torzillo, Giuseppe
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biological hydrogen production is being evaluated for use as fuel, since it is a promising substitute for carbonaceous fuels owing to its high conversion efficiency and high specific energy content. The microalga Chlamydomonas reinhardtii when grown under sulfur-deprived conditions, switches the metabolism toward the production of hydrogen. A better understandings of physiological and biochemical changes occurring during each phases of the process, represents a prerequisite to enhance the hydrogen output. The aim of this work was to study whether the activation of enzymes of the antioxidant defense system, such as catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (GPOX), takes place during the entire process of hydrogen production by C. reinhardtii CC 124. Kinase activities present in the crude protein extract and the mitotic specific ones associated with CKS1 protein were assayed to determine how the conditions leading to hydrogen production affected the activities of mitotic and growth associated kinases. We present evidences that oxidative stress enzymes are active during the entire hydrogen production process, besides their activities are higher in the anoxic phase. Stress condition during hydrogen photoproduction provoked at least partial cell cycle arrest leading to block of mitosis and cell division. These findings are in line with the known down-regulation or block of cell cycle related processes in stressed or starved cells.
Fil: Sáenz, María Elena. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bišová, Kateřina. Czech Academy of Sciences; República Checa
Fil: Touloupakis, Eleftherios. Istituto per lo Studio degli Ecosistemi; Italia
Fil: Faraloni, Cecilia. Istituto per lo Studio degli Ecosistemi; Italia
Fil: Di Marzio, Walter Dario. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torzillo, Giuseppe. Istituto per lo Studio degli Ecosistemi; Italia
Materia
Biohydrogen
Chlamydomonas Reinhardtii
H2 Production
Kinase
Oxidative Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38463
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38463
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtiiSáenz, María ElenaBišová, KateřinaTouloupakis, EleftheriosFaraloni, CeciliaDi Marzio, Walter DarioTorzillo, GiuseppeBiohydrogenChlamydomonas ReinhardtiiH2 ProductionKinaseOxidative Stresshttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Biological hydrogen production is being evaluated for use as fuel, since it is a promising substitute for carbonaceous fuels owing to its high conversion efficiency and high specific energy content. The microalga Chlamydomonas reinhardtii when grown under sulfur-deprived conditions, switches the metabolism toward the production of hydrogen. A better understandings of physiological and biochemical changes occurring during each phases of the process, represents a prerequisite to enhance the hydrogen output. The aim of this work was to study whether the activation of enzymes of the antioxidant defense system, such as catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (GPOX), takes place during the entire process of hydrogen production by C. reinhardtii CC 124. Kinase activities present in the crude protein extract and the mitotic specific ones associated with CKS1 protein were assayed to determine how the conditions leading to hydrogen production affected the activities of mitotic and growth associated kinases. We present evidences that oxidative stress enzymes are active during the entire hydrogen production process, besides their activities are higher in the anoxic phase. Stress condition during hydrogen photoproduction provoked at least partial cell cycle arrest leading to block of mitosis and cell division. These findings are in line with the known down-regulation or block of cell cycle related processes in stressed or starved cells.Fil: Sáenz, María Elena. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bišová, Kateřina. Czech Academy of Sciences; República ChecaFil: Touloupakis, Eleftherios. Istituto per lo Studio degli Ecosistemi; ItaliaFil: Faraloni, Cecilia. Istituto per lo Studio degli Ecosistemi; ItaliaFil: Di Marzio, Walter Dario. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Torzillo, Giuseppe. Istituto per lo Studio degli Ecosistemi; ItaliaPergamon-Elsevier Science Ltd2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38463Sáenz, María Elena; Bišová, Kateřina; Touloupakis, Eleftherios; Faraloni, Cecilia; Di Marzio, Walter Dario; et al.; Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii; Pergamon-Elsevier Science Ltd; International Journal of Hydrogen Energy; 40; 33; 9-2015; 10410-104170360-3199CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijhydene.2015.06.124info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0360319915016092info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:37Zoai:ri.conicet.gov.ar:11336/38463instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:37.906CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii
title Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii
spellingShingle Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii
Sáenz, María Elena
Biohydrogen
Chlamydomonas Reinhardtii
H2 Production
Kinase
Oxidative Stress
title_short Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii
title_full Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii
title_fullStr Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii
title_full_unstemmed Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii
title_sort Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii
dc.creator.none.fl_str_mv Sáenz, María Elena
Bišová, Kateřina
Touloupakis, Eleftherios
Faraloni, Cecilia
Di Marzio, Walter Dario
Torzillo, Giuseppe
author Sáenz, María Elena
author_facet Sáenz, María Elena
Bišová, Kateřina
Touloupakis, Eleftherios
Faraloni, Cecilia
Di Marzio, Walter Dario
Torzillo, Giuseppe
author_role author
author2 Bišová, Kateřina
Touloupakis, Eleftherios
Faraloni, Cecilia
Di Marzio, Walter Dario
Torzillo, Giuseppe
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biohydrogen
Chlamydomonas Reinhardtii
H2 Production
Kinase
Oxidative Stress
topic Biohydrogen
Chlamydomonas Reinhardtii
H2 Production
Kinase
Oxidative Stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Biological hydrogen production is being evaluated for use as fuel, since it is a promising substitute for carbonaceous fuels owing to its high conversion efficiency and high specific energy content. The microalga Chlamydomonas reinhardtii when grown under sulfur-deprived conditions, switches the metabolism toward the production of hydrogen. A better understandings of physiological and biochemical changes occurring during each phases of the process, represents a prerequisite to enhance the hydrogen output. The aim of this work was to study whether the activation of enzymes of the antioxidant defense system, such as catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (GPOX), takes place during the entire process of hydrogen production by C. reinhardtii CC 124. Kinase activities present in the crude protein extract and the mitotic specific ones associated with CKS1 protein were assayed to determine how the conditions leading to hydrogen production affected the activities of mitotic and growth associated kinases. We present evidences that oxidative stress enzymes are active during the entire hydrogen production process, besides their activities are higher in the anoxic phase. Stress condition during hydrogen photoproduction provoked at least partial cell cycle arrest leading to block of mitosis and cell division. These findings are in line with the known down-regulation or block of cell cycle related processes in stressed or starved cells.
Fil: Sáenz, María Elena. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bišová, Kateřina. Czech Academy of Sciences; República Checa
Fil: Touloupakis, Eleftherios. Istituto per lo Studio degli Ecosistemi; Italia
Fil: Faraloni, Cecilia. Istituto per lo Studio degli Ecosistemi; Italia
Fil: Di Marzio, Walter Dario. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torzillo, Giuseppe. Istituto per lo Studio degli Ecosistemi; Italia
description Biological hydrogen production is being evaluated for use as fuel, since it is a promising substitute for carbonaceous fuels owing to its high conversion efficiency and high specific energy content. The microalga Chlamydomonas reinhardtii when grown under sulfur-deprived conditions, switches the metabolism toward the production of hydrogen. A better understandings of physiological and biochemical changes occurring during each phases of the process, represents a prerequisite to enhance the hydrogen output. The aim of this work was to study whether the activation of enzymes of the antioxidant defense system, such as catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (GPOX), takes place during the entire process of hydrogen production by C. reinhardtii CC 124. Kinase activities present in the crude protein extract and the mitotic specific ones associated with CKS1 protein were assayed to determine how the conditions leading to hydrogen production affected the activities of mitotic and growth associated kinases. We present evidences that oxidative stress enzymes are active during the entire hydrogen production process, besides their activities are higher in the anoxic phase. Stress condition during hydrogen photoproduction provoked at least partial cell cycle arrest leading to block of mitosis and cell division. These findings are in line with the known down-regulation or block of cell cycle related processes in stressed or starved cells.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38463
Sáenz, María Elena; Bišová, Kateřina; Touloupakis, Eleftherios; Faraloni, Cecilia; Di Marzio, Walter Dario; et al.; Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii; Pergamon-Elsevier Science Ltd; International Journal of Hydrogen Energy; 40; 33; 9-2015; 10410-10417
0360-3199
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38463
identifier_str_mv Sáenz, María Elena; Bišová, Kateřina; Touloupakis, Eleftherios; Faraloni, Cecilia; Di Marzio, Walter Dario; et al.; Evidences of oxidative stress during hydrogen photoproduction in sulfur-deprived cultures of Chlamydomonas reinhardtii; Pergamon-Elsevier Science Ltd; International Journal of Hydrogen Energy; 40; 33; 9-2015; 10410-10417
0360-3199
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijhydene.2015.06.124
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0360319915016092
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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