Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions

Autores
Sabatini, Sebastian Eduardo; Leonardi, Patricia Ines; Rodríguez, María C.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to determine the responses to sublethal copper exposure (20 and 40 mM CuCl) in two Chlamydomonas reinhardtii strains: the wild type 4A+and the pleiotropic mutant cur9, originally selected as a copper-tolerant phenotype in mixotrophic conditions. Bioassays were performed in both autotrophic and mixotrophic media, under low irradiation (50 mol -2-1). At the onset, Cu2+ concentration in culture media was estimated by MINEQL software. Oxidative stress indicators, such as changes in photosynthetic pigment ratios, catalase activity, concentration of thiobartituric acid reactive substances (TBARS) and growth rate demonstrated a better performance of the wild type and the mutant strain in autotrophic and mixotrophic conditions, respectively. Moderate oxidative stress was accompanied by ultrastructural changes, such as accumulation of starch and vacuoles with electronic-dense deposits and membranous structures together with mild thylakoidal and mitochondrial crest disorganization. In mixotrophic conditions isocitrate lyase (ICL) activity peaked after 72-hour incubation. An inverse relation between catalase and ICL activities was verified and proved to be a good indicator of oxidative stress correlating consistently with other characteristic features such as, depression in growth rate or increase in TBARS dosage.
Fil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Rodríguez, María C.. Universidad de Buenos Aires; Argentina
Materia
Chlamydomonas Reinhardtii
Oxidative Stress
Autotrophic And Mixotrophic Growth
Lipid Peroxidation
Catalase
Isocitrate Ivase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20148
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/20148
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditionsSabatini, Sebastian EduardoLeonardi, Patricia InesRodríguez, María C.Chlamydomonas ReinhardtiiOxidative StressAutotrophic And Mixotrophic GrowthLipid PeroxidationCatalaseIsocitrate Ivasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work was to determine the responses to sublethal copper exposure (20 and 40 mM CuCl) in two Chlamydomonas reinhardtii strains: the wild type 4A+and the pleiotropic mutant cur9, originally selected as a copper-tolerant phenotype in mixotrophic conditions. Bioassays were performed in both autotrophic and mixotrophic media, under low irradiation (50 mol -2-1). At the onset, Cu2+ concentration in culture media was estimated by MINEQL software. Oxidative stress indicators, such as changes in photosynthetic pigment ratios, catalase activity, concentration of thiobartituric acid reactive substances (TBARS) and growth rate demonstrated a better performance of the wild type and the mutant strain in autotrophic and mixotrophic conditions, respectively. Moderate oxidative stress was accompanied by ultrastructural changes, such as accumulation of starch and vacuoles with electronic-dense deposits and membranous structures together with mild thylakoidal and mitochondrial crest disorganization. In mixotrophic conditions isocitrate lyase (ICL) activity peaked after 72-hour incubation. An inverse relation between catalase and ICL activities was verified and proved to be a good indicator of oxidative stress correlating consistently with other characteristic features such as, depression in growth rate or increase in TBARS dosage.Fil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaFil: Rodríguez, María C.. Universidad de Buenos Aires; ArgentinaInt Phycological Soc2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20148Sabatini, Sebastian Eduardo; Leonardi, Patricia Ines; Rodríguez, María C.; Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions; Int Phycological Soc; Phycologia; 50; 1; 1-2011; 78-880031-8884CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2216/09-101.1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:43Zoai:ri.conicet.gov.ar:11336/20148instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:43.979CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
title Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
spellingShingle Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
Sabatini, Sebastian Eduardo
Chlamydomonas Reinhardtii
Oxidative Stress
Autotrophic And Mixotrophic Growth
Lipid Peroxidation
Catalase
Isocitrate Ivase
title_short Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
title_full Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
title_fullStr Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
title_full_unstemmed Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
title_sort Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
dc.creator.none.fl_str_mv Sabatini, Sebastian Eduardo
Leonardi, Patricia Ines
Rodríguez, María C.
author Sabatini, Sebastian Eduardo
author_facet Sabatini, Sebastian Eduardo
Leonardi, Patricia Ines
Rodríguez, María C.
author_role author
author2 Leonardi, Patricia Ines
Rodríguez, María C.
author2_role author
author
dc.subject.none.fl_str_mv Chlamydomonas Reinhardtii
Oxidative Stress
Autotrophic And Mixotrophic Growth
Lipid Peroxidation
Catalase
Isocitrate Ivase
topic Chlamydomonas Reinhardtii
Oxidative Stress
Autotrophic And Mixotrophic Growth
Lipid Peroxidation
Catalase
Isocitrate Ivase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this work was to determine the responses to sublethal copper exposure (20 and 40 mM CuCl) in two Chlamydomonas reinhardtii strains: the wild type 4A+and the pleiotropic mutant cur9, originally selected as a copper-tolerant phenotype in mixotrophic conditions. Bioassays were performed in both autotrophic and mixotrophic media, under low irradiation (50 mol -2-1). At the onset, Cu2+ concentration in culture media was estimated by MINEQL software. Oxidative stress indicators, such as changes in photosynthetic pigment ratios, catalase activity, concentration of thiobartituric acid reactive substances (TBARS) and growth rate demonstrated a better performance of the wild type and the mutant strain in autotrophic and mixotrophic conditions, respectively. Moderate oxidative stress was accompanied by ultrastructural changes, such as accumulation of starch and vacuoles with electronic-dense deposits and membranous structures together with mild thylakoidal and mitochondrial crest disorganization. In mixotrophic conditions isocitrate lyase (ICL) activity peaked after 72-hour incubation. An inverse relation between catalase and ICL activities was verified and proved to be a good indicator of oxidative stress correlating consistently with other characteristic features such as, depression in growth rate or increase in TBARS dosage.
Fil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Rodríguez, María C.. Universidad de Buenos Aires; Argentina
description The aim of this work was to determine the responses to sublethal copper exposure (20 and 40 mM CuCl) in two Chlamydomonas reinhardtii strains: the wild type 4A+and the pleiotropic mutant cur9, originally selected as a copper-tolerant phenotype in mixotrophic conditions. Bioassays were performed in both autotrophic and mixotrophic media, under low irradiation (50 mol -2-1). At the onset, Cu2+ concentration in culture media was estimated by MINEQL software. Oxidative stress indicators, such as changes in photosynthetic pigment ratios, catalase activity, concentration of thiobartituric acid reactive substances (TBARS) and growth rate demonstrated a better performance of the wild type and the mutant strain in autotrophic and mixotrophic conditions, respectively. Moderate oxidative stress was accompanied by ultrastructural changes, such as accumulation of starch and vacuoles with electronic-dense deposits and membranous structures together with mild thylakoidal and mitochondrial crest disorganization. In mixotrophic conditions isocitrate lyase (ICL) activity peaked after 72-hour incubation. An inverse relation between catalase and ICL activities was verified and proved to be a good indicator of oxidative stress correlating consistently with other characteristic features such as, depression in growth rate or increase in TBARS dosage.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20148
Sabatini, Sebastian Eduardo; Leonardi, Patricia Ines; Rodríguez, María C.; Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions; Int Phycological Soc; Phycologia; 50; 1; 1-2011; 78-88
0031-8884
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20148
identifier_str_mv Sabatini, Sebastian Eduardo; Leonardi, Patricia Ines; Rodríguez, María C.; Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions; Int Phycological Soc; Phycologia; 50; 1; 1-2011; 78-88
0031-8884
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2216/09-101.1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Int Phycological Soc
publisher.none.fl_str_mv Int Phycological Soc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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